Browse > Article

Structural Identification of $Siderophore_{AH18}$ from Bacillus subtilis AH18, a Biocontrol agent of Phytophthora Blight Disease in Red-pepper  

Woo, Sang-Min (Department of Applied Microbiology, Yeungnam University)
Kim, Sang-Dal (Department of Applied Microbiology, Yeungnam University)
Publication Information
Microbiology and Biotechnology Letters / v.36, no.4, 2008 , pp. 326-335 More about this Journal
Abstract
The siderophore ($siderophore_{AH18}$) of Bacillus subtilis AR18 was determined to be one of catechol type and purified by using Amberlite XAD-2, Sephadex LR-20 chromatography, and reversed-phase RPLC. The $Siderophore_{AH18}$ was identified bacillibactin with its structure by GC-MS, $^1H$-NMR, and $^{13}C$-NMR. $Siderophore_{AH18}$ (bacillibactin) had been confirmed its molecular weight of 883 and chemical structure of $(2,3-dihydroxybenzoate-glycine-threonine)_3$. Purified $siderophore_{AH18}$ showed strong biocontrol ability towards the spore of Phytophthora capsici on PDA and able to effectively suppress (55%) P. capsici causing red-pepper blight in the pot in vivo test.
Keywords
Bacillibactin; PGPR (plant growth promoting rhizobacterium); siderophore; Bacillus subtilis AH18;
Citations & Related Records
Times Cited By KSCI : 15  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Glick, B. R., C. L. Patten, G. Patten, and D. M. Penrose. 1999. Biochemical and genetic mechanisms used by plant growth promoting bacteria. Imperial College Press. Canada
2 Han, K. H., C. U. Lee, and S. D. Kim. 1999. Antagonistic role of chitinase and antibiotic produced by Promicromonospora sp. KH-28 toward F. oxysporum. Kor. J. Appl. Microbial. Biotechnol. 27: 349-353
3 Jung, H. K., J. C. Ryoo, and S. D. Kim. 2005. A multimicrobial biofungicide for the biological control against several important plant pathogenic fungi. J. Kor. Soc. Appl. Biol. Chem. 48: 40-47   과학기술학회마을
4 Lee, E. T., and S. D. Kim. 2000. Selection and antifungal activity of antagonistic bacterium Pseudomonas sp. 2112 against red-pepper rotting Phytophthora capsici. Kor. J. Appl. Microbiol. Biotechn. 28: 334-340   과학기술학회마을
5 Sturtevant, D. B. and B. J. Taller. 1989. Cytokinin production by Rhizobium japonicum. Plant Physiol. 39: 1247-1452
6 van Loon, L. C., P. A. H. M. Bakker, and C. M. Pieterse. 1998. Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathology. 36: 453-483   DOI   ScienceOn
7 Weinberg, E. D. 1974. Iron and susceptibility to infectious disease. Science. 184: 952-956   DOI
8 Hider, R. C. 1984. Siderophore mediated absorption of iron. Struct. Bonding. 58: 25-87   DOI
9 Lim, H. S. and S. D. Kim. 1997. Role of siderophore in biocontrol of Fusarium solani and enhanced growth response of Bean by Pseudomonas fluorescens GL20. J. Microbiol. Biotechnol. 7: 13-20
10 Yun, G. H., E. T. Lee, and S. D. Kim. 2001. Identification and antifungal antagonism of Chryseomonas luteola 5042 against Phytophthora capsici. Kor. J. Appl. Microbial. Biotechnol. 29: 186-193   과학기술학회마을
11 Woo, S. M., H. K. Jung, and S. D. Kim. 2006. Cloning and Characterization of a cellulase gene from a plant growth promoting rhizobacterium, Bacillus subtilis AH18 against phytophthora blight disease in red-pepper. Kor. J. Microbial. Biotechn. 34: 311-317   과학기술학회마을
12 Schyn, B. and J. B. Neilands. 1987. Universal chemical assay for the detection and determination of siderophore. Anal. Biochem. 160: 47-56   DOI   ScienceOn
13 Temirov, Y. V., T. Z. Esikova, I. A. Kashparov, T. A. Balashova, L. M. Vinokurov, and Y. B. Vinokurov. 2006. A catecholic siderophore produced by the thermoresistant Bacillus licheniformis VK21 strain. Rus. J. Bioorg. Chem. 26: 542-549
14 Bergeron, R. J., and J. S. McManis. 1991. Synthesis of catecholamide and hydroxamate siderophore. In CRC Hanbook of Microbial Iron Chelates, (ed) G. Winkelmann, CRC Press, Boca Raton, F1. pp. 271-307
15 Crosa, J. H. 1989. Genetics and molecular biology of siderophore-mediated iron transport in bacteria. Microbiol. Rev. 53: 517-530   PUBMED
16 Payne, S. M. 1994. Detection, isolation, and characterization of siderophore. Method. Enzymol. 235: 329-344   DOI
17 Woo, S. M., J. U. Woo, and S. D. Kim. 2007. Purification and characterization of the sidrophore from Bacillus licheniformis K11, a multi-functional plant growth promoting rhizobacterium. Kor. J. Microbial. Biotechn. 35: 128-134   과학기술학회마을
18 Lee, J. M., H. S. Lim, T. H. Chang, and S. D. Kim. 1999. Isolation of siderophore-producing Pseudomonas fluorescens GL7 and its biocontrol activity against root-rot disease. Kor. J. Appl. Microbiol. Biotechn. 27: 427-432   과학기술학회마을
19 Lee S. Y., S. B. Lee, Y. K. Kim, and H. G. Kim. 2004. Effect of agrochemicals on mycelial growth and spore germination of a hyperparasite, Ampelomyces quisqualis 94013 for controlling cucumber powdery mildew. Kor. J. Pesti. Sci. 8: 71-78
20 Lim, H. S., J. M. Lee, and S. D. Kim. 2002. A plant growthpromoting Pseudomonas fluorescens GL20: Mechanism for disease suppression, outer membrane receptors for ferric siderophore, and genetic improvement for increased biocontrol efficacy. J. Microbiol. Biotechnol. 12: 249-257
21 Jung, H. K., J. R. Kim, S. M. Woo, and S. D. Kim. 2006. Selection of the auxin, siderophore, and cellulase-producing PGPR, Bacillus licheniformis K11 and its plant growth promoting mechanisms. J. Kor. Soc. Appl. Biol. Chem. 50:23-28   과학기술학회마을
22 Csaky, T. 1948. On the estimation of bound hydroxylamine. Acta Chem. Scand. 2: 450-454   DOI
23 Kwon, D. H., J. H. Choe, H. K. Jeong, J. H. Lim, G. J. Ju, and S. D. Kim. 2004. Selection and identification of auxinproducing plant growth promoting rhizobacteria having phytopathogen antagonistic activity. J. Kor. Soc. Appl. Biol. Chem. 47: 17-21
24 Kim. K. Y., and S. D. Kim. 1997. Biological control of Pyricularia aryzae blast spot with the antibiotic substances produced by Bacillus sp. KL-3. Kor. J. Appl. Microbiol. Biotechnol. 25: 396-402
25 Lim, H. S. and S. D. Kim 1995. The role and characterization of ${\beta}-1,3-glucanase$ in biocontrol of Fusarium solani by Pseudomonas stutzeri. J. Microbiol. 33: 295-304   과학기술학회마을
26 Liu, L., J. W. Kloepper, and S. Tuzun. 1995. Induction of systemic resistance in cucumber by plant growth-promoting rhizobacteria: duration of protection and effect of host resistance on protection and root colonization. Phytopathology. 85: 1064-1068   DOI
27 Woo, S. M. and S. D. Kim. 2007. Confirmation of nonsiderophore antifungal substance and cellulase from Bacillus licheniformis K11 containing antagonistic ability and plant growth promoting activity. J. Life. Science. 17: 983-989   DOI
28 Lee, M. W. 1997. Root colonization by beneficial Pseudomonas spp. and bioassay of suppression of Fusarium wilt of radish. The Kor. J. Mycol. 25: 10-20   과학기술학회마을
29 Schottel, J. L., K. Shimizu, and L. L. Kinkel. 2001. Relationships of in vitro pathogen inhibition and soil colonization to potato scab biocontrol by antagonistic Streptomyces spp. Biol. Control. 20: 102-112   DOI   ScienceOn
30 Arnow, L. E. 1937. Colorimetric determination of the components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. J. Biol. Chem. 118: 531-537
31 Ping, L., and W. Boland. 2004. Signals from the underground: bacrerial volatiles promote growth in Arabidopsis. Trends Pla. Sic. 9: 263-266   DOI   ScienceOn
32 Imamura, N., T. Ishikawa, T. Ohtsuka, K. Yamamoto, M. Dekura, H. Fukami, and R. Nishida. 2000. An antibiotic from Penicillium sp. covering the cocoon of the leaf-rolling moth, dactylioglypha tonica. Biosci. Biotech. Bioch. 64: 2216-2217   DOI   ScienceOn
33 Jung, H. K., J. R. Kim, S. M. Woo, and S. D. Kim. 2006. An auxin producing plant growth promoting rhizobacterium Bacillus subtilis AH18 which has siderophore-Producing biocontrol activity. Kor. J. Microbiol. Biotechnol. 34: 94-100   과학기술학회마을
34 Neilands, J. B. 1984. Siderophores of bacteria and fungi. Microbiol. Sci. 1: 9-14   PUBMED
35 Paulitz, T. C. and J. E. Loper. 1991. Lack of a role for fluorescent siderphore production in the biological control of Phythium damping-off of cucumber by a strain of Pseudomonas putida. Phytopathology. 81: 930-935   DOI
36 Lee, J. M., E. S. Do, S. B. Baik, and S. C. Chun. 2003. Effect of organic amendments on efficacy of biological control of seedling damping-off of cucumber with several microbial products. The Kor. J. Mycol. 31: 44-49   과학기술학회마을   DOI
37 Jeong, D. H., K. D. Park, S. H. Kim, K. R. Kim, S. W. Choi, J. T. Kim, K. H. Choi, and J. H. Kim. 2004. Identification of Streptomyces sp. producing antibiotics against phytopathogenic fungi, and its structure. J. Microbiol. Biotechnol. 14: 212-215   과학기술학회마을
38 Katiyar, V., and G. Reeta. 2004. Improved plant growth from seed bacterization using siderophore overproducing cold resistant mutant of Pseudomonas fluorescens. J. Microbiol. Biotechnol. 14: 653-657   과학기술학회마을
39 Lee, I. K., C. J. Kim, S. D. Kim, and I. D. Yoo. 1990. Antifungal antibiotic against fruit rot disease of red pepper form Streptomyces parvullus. Kor. J. Appl. Microbiol. Biotechn. 18: 142-147
40 Miethke, M., O. Klots, U. Linne, J. J. May, C. L. Beckering, and M. A. Marahiel. 2006. Ferri-bacillibactin uptake and hydrolysis in Bacillus subtilis. Mol. Microbiol. 61: 1413-1427   DOI   ScienceOn
41 Scher, F. M., and R. Baker. 1982. Effect of Pseudomonas putida and a synthetic iron chelator on induction of soil suppressiveness to Fusarium wilt pathogens. Phytopathology. 72: 1567-1573   DOI
42 Jung, H. K., and S. D. Kim. 2004. Selection and antaginistic mechanism of Pseudomonas fluorescens 4059 against phytophthora blight disease. Kor. J. Microbiol. Biotechnol. 32: 312-316
43 Kang, S. J., J. H. Kim, and G. J. Joo. 2005. Isolation of antagonistic bacteria against Fusarium oxysporum and physicochemical properties of compost mixed with microbial formulation. Kor. J. Hort. Sci. Technol. 23: 342-350
44 Leoffler, W. J., S. M. Tschen, N. Vanittanakom, M. Kugler, E. Knorpp, T. F. Hsieh, and T. G. Wu. 1986. Antifungal effects of bacilysin and fengymycin from Bacillus subtilis F29-3: a comparison with activaties of other Bacillus antibiotics. J. Phytopathol. 115: 204-213   DOI
45 Garner, B. L., J. E. L. Arceneaux, and B. Rowe Byers. 2004. Temperature control of 3,4-dihydroxybenzoate (potocatechuate)- based siderophore in Bacillus anthracis. Curr. Microbiol. 49: 89-94   PUBMED