• Korean Journal of Food Science and Technology
• /
• v.46 no.3
• /
• pp.288-294
• /
• 2014

Penicillin and its salts, including the benzatine, procaine, and sodium salts, have been widely used in human and veterinary medicine. Owing to their low toxicity, they currently form the most important group of antibiotics. However, overdose and abuse of these antibiotics may lead to potential risk in human health. Therefore, this study was conducted to validate the analysis method established by the Korea Food Code in 2012 and to monitor the levels of benzylpenicillin residues in products with reference to the maximum residue level (MRL). Of the 232 product samples tested, benzylpenicillin was detected in 11 livestock products and 2 marine products. Benzylpenicillin concentrations were found to be lower than the MRL in 12 products; however, the concentration of benzylpenicillin was found to be greater than the MRL in 1 pork product. The limit of quantification (LOQ) for benzylpenicillin was found to be 0.001-0.002 mg/kg, with an average recovery of 90.4-115.3%. Calibrations showed good linearity of 0.995 over a range of 0.002-0.05 mg/kg.

• Analytical Science and Technology
• /
• v.22 no.4
• /
• pp.319-325
• /
• 2009

An effective and specific procedure for confirmation of neomycin, aminoglycoside antibiotic in honey was developed and validated. Honey was adjusted to pH 2 with 0.1M HCl and applied to weak cation-exchange SPE cartridge. Neomycin was eluted with basified methanol. Following separation by ion-pairing liquid chromatography, neomycin was analysed with positive electrospray ionization and MRM mode. Quantification was linear over the range of $5.0{\sim}250.0{\mu}g/kg$ ($r^2$ >0.9951). The precision (R.S.D.) and accuracy (as a bias) of quality control samples in honey ranged 11.5~18.7% and 10.9~20.9%, respectively. Established method can be applied to analysis of neomycin in honey.

• Journal of fish pathology
• /
• v.34 no.1
• /
• pp.71-80
• /
• 2021

Cephalexin, a semi-synthetic cephalosporin antibiotic, has long been used in fish aquaculture in various countries under legal authorization. The drug is thus widely available for use in other aquatic species except fishes like the crustacean whiteleg shrimp. This study aims to develop a sensitive method for laboratory residue studies to adopt in withdrawal period determinations. Through repeated trials from the existing methods developed for other food animal tissues, it was possible to achieve a sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. The results showed that at a concentration of 0.1 mg/kg, the recovery rate was 81.79%, and C.V. value was 8.2%, which meet the recovery rate and C.V. recommended by Codex guideline. After satisfactory validation of analytical procedures, applicability to the shrimp tissue was confirmed in experimentally cephalexin-treated whiteleg shrimp. As a result, most muscle samples were detected below the limit of quantification (0.05 mg/kg) after day 3, and most hepatopancreas samples were detected below the limit of quantification after day 14. In particular, the limit of quantification 0.05 ppm with the presently developed method suggests sufficient sensitive over the current legal maximum residue limit of 0.2 mg/kg set for fishes.

• Korean Journal of Environmental Agriculture
• /
• v.35 no.3
• /
• pp.166-174
• /
• 2016

BACKGROUND: The current study developed a monitoring method of 6 veterinary antibiotics (amoxicillin, ampicillin, enrofloxacin, tetracycline, chlortetracycline, oxytetracycline) in agricultural soils using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in positive electrospray ionization mode.METHODS AND RESULTS: Sample preparation was carried out using acidic acetonitrile and citrate salts followed by purification with dispersive solid phase extraction (d-SPE). Separation on Eclipse Plus C₁₈ column was conducted in gradient of the mobile phase, 0.1% formic acid and 5 mM ammonium formate in methanol (A) and 0.1% formic acid and 5 mM ammonium formate in distilled water (B). The linearity of the matrix-matched calibrations expressed as the coefficient of determination was good with R²≥0.9900. The limit of quantifications (LOQs) ranged from 0.5 to 10 μg/kg for all analytes. Analysis of 51 agricultural soil samples taken in the Republic of Korea revealed concentrations less than 1.9 μg/kg for enrofloxacin, 75.5 μg/kg for chlortetracycline.CONCLUSION: The method was successfully applied to monitor 6 veterinary antibiotics from 51 field incurred agricultural soil samples in 17 provincial areas throughout the Republic of Korea. The developed method was simple, easy, and versatile and can be used for monitoring various veterinary antibiotics in soil.

• Journal of fish pathology
• /
• v.22 no.1
• /
• pp.53-65
• /
• 2009

Tenacibaculum maritimum (formerly Flexibacter maritimus) is the aetiological agent of an ulcerative and necrotic disease commonly called tenacibaculosis in marine fish. Tenacibaculosis is an economically important disease in a great variety species in Jeju Island cultured fish and leading to this pathogen initially affected by skin, mouth, fins, tail causing severe necrotic and ulcerative lesions on the body surface. In the present study, A-7 strain was isolated from Paralichthys olivaceus showing symptoms of tenacibaculosis and identified as T. maritimum by morphological, biochemical and molecular biological analysis. T. maritimum A-7 is experimentally infected through immersion route in Paralichthys olivaceus which the disease outbreaks in land-based fish tanks of Jeju Island. Up to data a number of treatments proposed for the tenacibaculosis outbreaks are based on the immersion administration of drugs in tank. Oxytetracycline is the most widely used disinfectants in fish farms. However, most of fish farms manager and consumers have expressed concern as bioaccumulation in tissue and its environmental. In addition, this antimicrobial compounds is expensive in fish farmers. The overcome of this problem is desired the application of natural plant derived products. To obtain as 70% EtOH extract antimicrobial compounds against tenacibaculosis from 35 species of Jeju Island native plants were screened for antimicrobial activity against T. maritimum. In the present study were identified most of the plant extracts were better antimicrobial activity against T. maritimum.

• Microbiology and Biotechnology Letters
• /
• v.38 no.1
• /
• pp.77-83
• /
• 2010

The fructus of Crataegus pinnatifida Bunge (CBF) has been used as medicinal and food source in worldwide. In this study, antimicrobial activity of the methanol extract and its sequential organic solvent fractions of CBF against different pathogenic bacteria and fungi, including multidrug resistant Pseudomonas aeruginosa and Candida sp., were investigated. The methanol extract of CBF was active against various gram-positive and gram-negative bacteria, and the ethylacetate and butanol fractions of CBF showed strong antibacterial activity against Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium, Proteus vulgaris, Escherichia coli and various multidrug resistant Pseudomonas aeruginosa with minimal inhibitory concentration of 1.0~7.5 mg/mL. Also the fractions showed anti-Candida activity against C. albicans, C. kruseis and C. geochares. The methanol extract of CBF and its solvent fractions, except n-hexane fraction, did not show any hemolytic activity against human red blood cell up to $500\;{\mu}g/mL$, respectively. The hemolysis in n-hexane fraction at $500\;{\mu}g/mL$ was less than 9.9%. Our results suggest that the CBF could be developed as a potent antibacterial agent, especially for multidrug resistant Pseudomonas aeruginosa.

• 동물약계
• /
• no.97
• /
• pp.3-6
• /
• 2004

[ $\cdot$ ]동물약사감시 행정처분 결과 홍보 $\cdot$수입백신 특별소위원회 구성 $\cdot$축산용 항생제 관리시스템 구축 협의회 참석 $\cdot$동물약품업계 발전을 위한 정책토론회 참석 $\cdot$임시 이사회 개최 $\cdot$중국산 동물약품 GMP 관련 수입시 유의사항 $\cdot$가축전염병 예방약류 사양서 제출 $\cdot$3$\cdot$4/4분기 가축전염병 중앙예찰협의회 참석 $\cdot$부작용 수입 동물약품 회수 및 잠정 수입중단 $\cdot$현장문제 해결을 위한 연구과제 제안 $\cdot$검역원, 잔류물질 관리 국제 심포지엄 개최 $\cdot$대체의학 특별소위원회 개최 $\cdot$동물약품 사용 절감대책 추진회의 참석 $\cdot$동물약품제조용 양허관세적용 유당 변경 배정 $\cdot$산업자원부 해외전시회 단체참가 지원 신청 $\cdot$IFAH, 사료첨가제 감축 관련 의견 제시 $\cdot$제5회 KAHPA컵 골프대회 개최 $\cdot$조합 2004년도 하반기 알선품목 선정 $\cdot$2004 국제 애완동물 및 용품 전시회 개최

• Monthly Korean Chicken
• /
• v.2 no.7 s.13
• /
• pp.2-8
• /
• 1996

육류중 유해물질 검사 강화 - 육계 초기 사료제한 연구 - 잔류 항생제 정성검사 키트 개발 - 일본인 육류섭취량 증가추세 - 축산폐수 단속기중 강화 - 축산물 소비촉진 홍보대책 필요 - 사료용 부연료 할당관세 $2.5{\%}$적용 - 가금티푸스 예방약 개발 - 가금인플루엔자 방역대책 시급 - 종계 ND 발생지역 백신 프로그램 - 수입종란 외래질병 매개 가능성 - 안전축산물 생산운동연합회 발족 - 중국, 닭고기 산업 잠재력 무한 - 삼계탕 수출 급속 성장중 - 소비자 단체 임원 닭 생산현장 방문 - 산란노계 처리 계속 어려워 - 인도, 육계수입 규제완화 재검토 - 축산기자재 구입 외화자금 사용가능 - 미국, 도계전 급이중단 실험 - 러시아, 수입쿼터제 부과 - 호주, 브로일러 산업 지속적 성장 - 배합사료업체 사료원료 선물구매 기피

• The Korean Chronic Disease News
• /
• no.319
• /
• pp.1-18
• /
• 2007

복지시대의 첨병-구례군보건의료원 김영락 원장/노인복지시설 통합.개편/비만치료도 건강보험급여 대상/보건소 중심, 만성질환 관리시스템 구축/임의왕진 의료급여비 환수/적절한 운동이 '대장암' 예방한다/모유수유 오래하면 유방암 감소/50대 두통, 어지러움은 중풍 의심/축수산물 항생제 잔류 기준 엄격 적용/담배연기 없는 깨끗한 병원/레이저 이용 암 세포만 선택적 파괴/'주민 삶의 질 적인 개선' 지향-구례구보건의료원/농촌지역 공공보건의료 질적 발전에 정열 쏟아-김영락 원장/동아제약, 유럽 시장 본격진출/LG, 세계 최초 항구토 패치 도입/한미약품, 의약사 금융지원 한다/국내 첫 생약제제 임상시험 지침 마련/암환자 완치율 44.4% 수준/고혈압.당뇨병치료율 2배 증가/혈압 없어도 '코골면' 고혈압 위험 높아/조기위암 전체 위암 절반 넘었다/항암면역세포치료제 NKM 허가/OECD Health Data 2007 보건의료실태분석

• The Korea Swine Journal
• /
• v.18 no.6 s.202
• /
• pp.139-141
• /
• 1996

한국 축산업 발전을 위하여 축산물 자유화를 눈앞에 두고 농수산부에서는 국민건강 안전과 소비자들의 기호에 맞는 고급육 생산을 위하여 육류에 항생제 잔류물 없애기와 각종 세균수 줄이기에 앞다투어 노력하고 실행할 방침을 제시해 놓았고 그렇게 되도록 전국 양축가와 도축장 및 육가공육류 해체작업장이며 유통업무에 종사한 도소매상인들에 최고의 기술과 고뇌에 짜낸 결실을 기대하고 있던차 모든 과제의 원인제공인 가축질병박멸대책을 위해 여러 학계교수, 수의학전문의, 생산자단체, 축산업에 일생을 맡겨 분투해온 분들로 위원회를 구성하여 가축질병박멸대책안을 만들어 수차례의 협의끝에 그런대로의 최선과 최대의 안이 나와 곧 발표단계며 곧바로 시행에 들어갈 것이다. 한편은 걱정도 있다. 시행하다보면 다시 손질해야할 점도 돌출될 것이며 전국 양축가들이 값비싼 사료로 가축에게 100%의 결실이 있게 된다면 얼마나 좋겠는가? 좋은 결실을 위한 노력이니 좋은 열매가 하루속히 이루어질 것으로 믿는다.