Browse > Article

Antimicrobial Activity of Fruit of Crataegus pinnatifida Bunge against Multidrug Resistant Pathogenic Pseudomonas aeruginosa and Candida sp.  

Ryu, Hee-Young (Dept. of Food and Nutrition, Andong National University)
Ahn, Seon-Mi (Dept. of Food and Nutrition, Andong National University)
Kim, Jong-Sik (Dept. of Biological Science, School of Bioresource Science, Andong National University)
Jung, In-Chang (Dept. of Food and Nutrition, Andong National University)
Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.1, 2010 , pp. 77-83 More about this Journal
Abstract
The fructus of Crataegus pinnatifida Bunge (CBF) has been used as medicinal and food source in worldwide. In this study, antimicrobial activity of the methanol extract and its sequential organic solvent fractions of CBF against different pathogenic bacteria and fungi, including multidrug resistant Pseudomonas aeruginosa and Candida sp., were investigated. The methanol extract of CBF was active against various gram-positive and gram-negative bacteria, and the ethylacetate and butanol fractions of CBF showed strong antibacterial activity against Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Salmonella typhimurium, Proteus vulgaris, Escherichia coli and various multidrug resistant Pseudomonas aeruginosa with minimal inhibitory concentration of 1.0~7.5 mg/mL. Also the fractions showed anti-Candida activity against C. albicans, C. kruseis and C. geochares. The methanol extract of CBF and its solvent fractions, except n-hexane fraction, did not show any hemolytic activity against human red blood cell up to $500\;{\mu}g/mL$, respectively. The hemolysis in n-hexane fraction at $500\;{\mu}g/mL$ was less than 9.9%. Our results suggest that the CBF could be developed as a potent antibacterial agent, especially for multidrug resistant Pseudomonas aeruginosa.
Keywords
Antibacterial; multidrug resistant Pseudomonas aeruginosa; anti-Candida; fructus of Crataegus pinnatifida Bunge;
Citations & Related Records
Times Cited By KSCI : 13  (Citation Analysis)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 An, B. J., B. Y. Kang, and J. T. Lee. 2002. Development of cosmetic material from Korean Crataegi fructus extract. Kor. J. Herbology 17: 39-50.
2 Choi, O.-K., Y. Kim, G.-S. Cho, and C.-K. Sung. 2002. Screening for antimicrobial activity from korean plants. Kor. J. Food. Nutr. 15: 300-306.
3 Kang, H. C., K. K. Shim, Y. M. Ha, and W. H. Lee. 2002. New varieties with thronless branches of Crataegus pinnatifida Bunge. Kor. J. Hor. Sci. Technol. 20: 252-256.
4 Kang, I.-H., J.-H. Cha, S.-W. Lee, H.-J. Kim, S.-H. Kwon, I.-H. Ham, B.-S. Hwang, and W.-K. Whang. 2005. Isolation of anti-oxidant from domestic Crataegus pinnatifida Bunge leaves. Kor. J. Pharmacogn. 36: 121-128.
5 Lee, S. Y., J. H. An, H. Chun, and H. Y. Cho. 2003. Isolation and characterization of MMP-1 inhibitor peptide from Crataegus pinnatifida Bunge in fibroblast cell line HS68 cells. J. Kor. Sco. Agric. Chem. Biotechnol. 46: 60-65.
6 Park, S. W., C. S. Yook, and H. K. Lee. 1994. Chemical components from the fruits of Crataegus pinnatifida var psilosa. Kor. J. Pharmacogn. 25: 328-335.
7 Ryu, H. Y., S. J. Park, B. H. Lee, and H. Y. Sohn. 2007. Control of Yam-putrefactive psychrotrophic bacterium using clove oil and preparation of functional fresh-cut. Kor. J. Microbiol. Biotechnol. 35: 66-72.
8 Ryu, H. Y., Y. K. Kim, I. S. Kwun, C. S. Kwon, I. N. Ji,n and H. Y. Sohn. 2007. Thrombin inhibition activity of fructus extract of Crataegus pinnatifida Bunge. Kor. J. Life Sci. 17: 535-539.   DOI
9 Bae, M. H. and H. H. Kim. 2003. Mechanism of Crataegi fructus extract induced endothelium-dependent vasorelaxation in rabbit carotid artery. Kor. J. Herbology 18: 169-180.
10 Seo, B. I. 2005. Preventive effects of water extracts from on Crataegi fructus on hyperlipiderma and liver damage induced by alcohol. Kor. J. Herbology. 20: 35-43.
11 Sohn, H. Y., K. H. Son, C. S. Kwon, G. S. Kwon, and S. S. Kang. 2004. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morusalba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine 11: 666-672.
12 Singleton, V. L, R. Orthofer, and R. M. Lamuela-Raventos. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaleau reagent. Methods Enzymol 299: 152-178.   DOI
13 Jeong T. S., E. I. Hwang, H. B. Lee, E. S. Lee, Y. K. Kim, B. S. Min, K. H. Bae, S. H. Bok, and S. U. Kim. 1999. Chitin synthase II inhibitory activity of ursolic acid, isolated from Crataegus pinnatifida. Planta Med. 65: 261-263.   DOI
14 Park, C. G., K. H. Bang, S. E. Lee, M. S. Cha, J. S. Sung, H. W. Park, and N. S. Seong. 2001. Antibacterial activity from medicinal plant extracts on the Staphylococcus aureus. Kor. J. Med. Crop Sci. 9: 251-258.
15 Kim, J. S., G. D. Lee, J. H. Kwon, and H. S. Yeon. 1993. Antioxidative effectiveness of ether extract in Crataegus pinnatifida Bunge and Terminalia chebula Rets. J. Kor. Agric. Chem. Soc. 36: 203-207.
16 Woo, S. S., Y. K. Park, C. H. Choi, K. S. Hahm, and D. G. Lee. 2007. Mode of antibacterial action of a signal peptide, $Pep^{27}from$ Streptococcus pneumoniae. Biochem. Biophys. Res. Comm. 363: 806-810.   DOI   ScienceOn
17 Yun, J. S., B. H. Chung, N. Y. Kim, N. S. Seong, H. Y. Lee, J. H. Lee, and J. D. Kim. 2003. Screening of 94 plant species showing ACE inhibitory activity. Kor. J. Med. Crop. Sci. 11: 246-251.
18 An, B. J. and J. T. Lee. 2002. Studies on biological activity from extract of Crataegi fructus. Kor. J. Herbology 17: 29-38.
19 Hooi, D. S. W., B. W. Bycroft, S. R. Chhabra, P. Williams, and D. I. Pritchard. 2004. Differential immune modulatory activity of Pseudomonas aeruginosa quorum sensing signal molecules. Infect. Immun. 72: 6463-6470.   DOI   ScienceOn
20 El-Mousallamy, A. M. D., 1998. Chemical investigation of the constitutive flavonoid glycosides of the leaves of Crataegus sinaica. Natural Prod. Sci. 4: 53-57.
21 Kim J. I., H. S. Jang, J. S. Kim, and H. Y. Sohn. 2009. Evaluation of antimicrobial, antithrombin, and antioxidant activity of Dioscorea batatas Decne. Kor. J. Microbiol. Biotechnol. 37:133-139.
22 Min, B. S., Y. H. Kim, S. M. Lee, H. J. Jung, J. S. Lee, M. K. Ma, C. O. Lee, J. P. Lee, and K. Bae. 2000. Cytotoxic triterpenes from Crataegus pinnatifida. Arch. Pharm. Res. 23: 155-158.   DOI
23 Kao, E. S., C. J. Wang, W. L. Lin, Y. F. Yin, C. P. Wang, and T. H. Tseng. 2005. Anti-inflammatory potential of flavonoid contents from dried fruit of Crataegus pinnatifida in vitro and in vivo. J. Agric. Food. Chem. 53: 430-436.   DOI   ScienceOn
24 Zhang, P. C. and S. X. Xu. 2002. Two new C-glucoside flavonoids from leaves of Crataegus pinnatifida Bge var. major N. E. Br. Chinese Chem. Lett. 13: 337-340.
25 Ahn, K. S., M. S. Hahm, E. J. Park, H. K. Lee, and I. H. Kim. 1998. Corosolic acid isolated from the fruit of Crataegus pinnatifida var. psilosa is a protein kinase C inhibitor as well as a cytotoxic agent. Planta Med. 64: 468-470.   DOI   ScienceOn
26 Kim, J. S., G. D. Lee, J. H. Kwon, and H. S. Yeon. 1993. Identification of phenolic antioxidative components in Crataegus pinnatifida Bunge. J. Kor. Agric. Chem. Soc. 36: 154-157.
27 Lee, H. J., and M. S. Choi. 1998. Changes of plasma and hepatic lipids, hydroxy-methyl-glutaryl CoA reductase activity and acyl-CoA: cholesterol acyltransferase activity by supplementation of hot water extracts from Rosa rugosa, Crataegus pinnatifida and Polygon. J. Food. Sci. Nutr. 3: 344-350.
28 Zhang, P. C. and S. X. Xu. 2001. Flavonoid ketohexosefuranosides from the leaves of Crataegus pinnatifida Bge var. major N. E. Br. Phytochemistry 57: 1249-1253.   DOI   ScienceOn
29 Min, B. S., H. J. Jung, J. S. Lee, Y. H. Kim, S. H. Bok, C. M. Ma, N, Nakamura, M. Hattori, and K. Bae. 1999. Inhibitory effect of triterpenes from Crataegus pinatifida on HIV-I protease. Planta Med. 65: 374-375.   DOI
30 Hong, S. S., J. S. Hwang, S. A. Lee, X. H. Han, J. S. Ro, and K. S. Lee. 2002. Inhibitors of monoamine oxidase activity from the fruits of Crataegus pinnatifida Bunge. Kor. J. Pharmacogn. 33: 285-290.