Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Biological characterization of Tenacibaculum maritimum isolated from cultured olive flounder in Korea and sensitivity against native plant extracts

한국의 양식넙치에서 분리한 Tenacibaculum maritimum의 특성과 자생식물 추출물에 대한 감수성

  • 장영환 (제주대학교 해양과학대학 해양과학부) ;
  • 정준범 (제주대학교 해양과학대학 해양과학부) ;
  • 여인규 (제주대학교 해양과학대학 해양과학부) ;
  • 김기영 (제주대학교 해양과학대학 해양과학부) ;
  • ;
  • 허문수 (제주대학교 해양과학대학 해양과학부)
  • Published : 2009.04.01
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Abstract

Tenacibaculum maritimum (formerly Flexibacter maritimus) is the aetiological agent of an ulcerative and necrotic disease commonly called tenacibaculosis in marine fish. Tenacibaculosis is an economically important disease in a great variety species in Jeju Island cultured fish and leading to this pathogen initially affected by skin, mouth, fins, tail causing severe necrotic and ulcerative lesions on the body surface. In the present study, A-7 strain was isolated from Paralichthys olivaceus showing symptoms of tenacibaculosis and identified as T. maritimum by morphological, biochemical and molecular biological analysis. T. maritimum A-7 is experimentally infected through immersion route in Paralichthys olivaceus which the disease outbreaks in land-based fish tanks of Jeju Island. Up to data a number of treatments proposed for the tenacibaculosis outbreaks are based on the immersion administration of drugs in tank. Oxytetracycline is the most widely used disinfectants in fish farms. However, most of fish farms manager and consumers have expressed concern as bioaccumulation in tissue and its environmental. In addition, this antimicrobial compounds is expensive in fish farmers. The overcome of this problem is desired the application of natural plant derived products. To obtain as 70% EtOH extract antimicrobial compounds against tenacibaculosis from 35 species of Jeju Island native plants were screened for antimicrobial activity against T. maritimum. In the present study were identified most of the plant extracts were better antimicrobial activity against T. maritimum.

Tenacibaculum maritimum (formerly Flexibacter maritimus)은 tenacibaculosis라 불리며, 해산어에서 궤양 및 괴사성 증상을 나타내는 질병원인체 이다. Tenacibaculosis는 다양한 양식 어종에서 있어서 경제적 측면에서도 중요한 질병 중 하나이며, 피부, 입, 지느러미, 꼬리 등과 같은 어체 표면에 심각한 궤양과 괴사로 인한 손상을 유발시 킨다. 본 연구에서는 tenacibaculosis의 증상을 나타내는 넙치 병어로부터 A-7 균주를 분리하였고, 형태학적 특성, 생화학적 성상 및 유전자 분석 결과를 바탕으로 T. maritimum으로 동정하였으며, 침지법을 통하여 넙치에 인위적으로 감염되는 것을 확인하였다. Tenacibaculosis를 제어하기 위해 약욕에 의한 항생제 투여가 많이 이루어지며, 그 중 oxytetracycline이 주로 사용되고있다. 그러나, 대부분의 양식어민과 소비자들은항생제 잔류와 환경오염 등의 문제를 우려하고있으며, 비싼 항생제의 사용으로 인하여 양식어의 생산 비용도 높아지고 있다. 이러한 문제점들을 극복하기 위하여 본 연구에서는 천연 자생식물 추출물을 항생제 대체제로 사용하기 위한 연구를 수행하였다. 총 35 종류의 천연식물의 항균 활성을 검색하였고, 많은 천연식물 추출물이 T. maritimum에 대하여 높은 항균 효과를 나타낸다는 것을 밝혔다.

Keywords

References

  1. Alsina, M. and Blanch, A.R.: First isolation of Flexibacter maritimus from cultivated turbot (Scophthalmus maximus). Bull. Eur. Assoc. Fish Pathol., 13:157-160, 1993
  2. Bauer, A.W., Kirby, W.M., Sherris, J.C. and Turck, M.: Antibiotic susceptibility testing by a standardizied single disk method. Am. J. Clin. Pathol., 45:439-496, 1966
  3. Baxa, D.V., Kawai, K. and Kusuda, R.: Characteristics of gliding bacteria isolated from diseased cultured flounder, Paralichthys olivaceous. Fish Pathol., 21:251-258, 1986 https://doi.org/10.3147/jsfp.21.251
  4. Bernardet, J.F., Campbell, A.C. and Buswell, J.A.: Flexibacter maritimus is the agent of 'black patch necrosis' in Dover sole in Scotland. Dis. Aquat. Org., 8:233-237, 1990 https://doi.org/10.3354/dao008233
  5. Campbell, A.C. and Buswell, J.A.: An investigation into the bacterial aetiology of 'black patch necrosis' in Dover sole, Solea solea L. J. Fish Dis., 5:495-508, 1982 https://doi.org/10.1111/j.1365-2761.1982.tb00509.x
  6. Cepeda, C. and Santos, Y.: First isolation of Flexibacter maritimus from Farmed Senegalese sole (Solea senegalensis, Kaup) in spain. Bull. Eur. Assoc. Fish Pathol., 22:388-392, 2002
  7. Chen, M.F., Henry-Ford, D. and Groff, J.M.: Isolation and characterization of Flexibacter maritimus from marine fishes of California. J. Aquat. Anim. Health, 7:318-326, 1995 https://doi.org/10.1577/1548-8667(1995)007<0318:IACOMF>2.3.CO;2
  8. Devesa, S., Barja, J.L. and Toranzo, A.E.: Ulcerative skin and fin lesions in reared turbot, Scophthalmus maximus(L). J. Fish Dis., 12:323-333, 1989 https://doi.org/10.1111/j.1365-2761.1989.tb00321.x
  9. Frette, L., Jorgensen, N.O.G., Irming, H. and Kroer, N.: 'Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark.'Int. J. Syst. Evol. Microbiol., 54:519-524, 2004 https://doi.org/10.1099/ijs.0.02398-0
  10. Hansen, G.H., Bergh, O., Michaelsen, J. and Knappskog, D.: Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, ippoglossus hippoglossus L. Int. J. Syst. Bacteriol., 42:451-458, 1992 https://doi.org/10.1099/00207713-42-3-451
  11. Handlinger, J., Soltani, M. and Percival, S.: The pathology of Flexibacter maritimus in aquaculture species in Tasmania, Australia. J. Fish Dis., 20:159-168, 1997 https://doi.org/10.1046/j.1365-2761.1997.00288.x
  12. Heo, G.J., Shin, K.S. and Lee, M.H.: Diseases of aquaculture animals and prevention of drug residues. Kor. J. Food Hygiene, 7:7-19, 1992
  13. Kim, J.W., Jung, S.H., Park, M.A., Do, J.W., Choi, D.L., Jee, B.Y., Cho, M.Y., Kim, M.S., Lee, J.S., Lee, C.H., Bang, J.D., Park, M.S. and Seo, J.S.: Monitoring of pathogens in cultured Fish of Korea for the summer period from 2000 to 2006. J. Fish Pathol., 19: 207-214, 2006a
  14. Kim, S., Chung, H.S., Kang, S.J., Ha, J.Y., Jung, W.C., Heo, S.H., Shin, Y.W., Kim, K.W., Kim, D.G. and Lee, H.J.: Tissue distribution after dippin administration of oxytetracycline and tetracycline in olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red Sea bream (Pagrus major). J. Fish Pathol., 19:155-164, 2006b
  15. Powell, M., Carson, J. van. and Gelderen, R.: Experimental introduction of gill disease in Atlantic salmon smolts, Salmo salar, with Tenacibaculum maritimum. Dis. Aquat. Org., 61:179-185, 2004 https://doi.org/10.3354/dao061179
  16. McPhearson, R.M., DePaola, A., Zywno, S.R., Motes, J.M.L. and Guarino, A.M.: Antibiotic resistance in gram-negative bacteria from cultured catfish and aquaculture ponds. Aquaculture, 99:203-211, 1991 https://doi.org/10.1016/0044-8486(91)90241-X
  17. McVicar, A.H. and White, P.G.: Fin and skin necrosis of cultivated Dover sole Soles solea (L). J. Fish Dis., 2:557-562, 1979 https://doi.org/10.1111/j.1365-2761.1979.tb00418.x
  18. Min, S.K, Park. Y.K., Park, J.H., Jin, S.H. and Kim, K.W.: Screening of antibacterial activity from hot water extracts of indigenous plants. J. life science, 14: 951-962, 2004 https://doi.org/10.5352/JLS.2004.14.6.951
  19. Moon, Y.G, Choi, K.S., Lee, K.J., Kim, K.Y. and Heo, M.S.: Latic acid bacterials growth, antioxidant activities and antimocrobial activity on fish pathogenic bacteria by nagative plant extracts, Jeju island. Kor. J. Microbiol. Biotechnology, 35:210-219, 2007
  20. Ostland, V.E., Latrace, C., Morrison, D. and Ferguson, H.W.: Flexibacter maritimus associated with bacterial stomatitis in Atlantic salmon smolts reared in net-pens in British Columbia. J. Aquat. Anim. Health, 11:35-44, 1999 https://doi.org/10.1577/1548-8667(1999)011<0035:FMAWAB>2.0.CO;2
  21. Suzuki, M., Nakagawa, Y., Harayama, S. and Yamamoto, S.: Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int. J. Syst. vol. Microbiol., 51:1639-1652, 2001
  22. Toranzo, A.E., Magarinos, B. and Romalde, J.L.: A review of the main bacterial fish diseases in marineculture system. Aquaculture, 246:37-61, 2005 https://doi.org/10.1016/j.aquaculture.2005.01.002
  23. Wakabayashi, H., Hikida, M. and Masumura, K.: Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int. J. Syst. Bacteriol., 36:396-398, 1986 https://doi.org/10.1099/00207713-36-3-396