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http://dx.doi.org/10.5338/KJEA.2016.35.3.29

Monitoring of Veterinary Antibiotics in Agricultural Soils using Liquid Chromatography Coupled with Tandem Mass Spectrometry  

Lee, Young-Jun (Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Choi, Jeong-Heui (Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Chung, Hyung Suk (Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Lee, Han Sol (Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Park, Byung-Jun (Chemical Safety Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Science)
Kim, Jang-Eok (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
Shim, Jae-Han (Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
Publication Information
Korean Journal of Environmental Agriculture / v.35, no.3, 2016 , pp. 166-174 More about this Journal
Abstract
BACKGROUND: The current study developed a monitoring method of 6 veterinary antibiotics (amoxicillin, ampicillin, enrofloxacin, tetracycline, chlortetracycline, oxytetracycline) in agricultural soils using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in positive electrospray ionization mode.METHODS AND RESULTS: Sample preparation was carried out using acidic acetonitrile and citrate salts followed by purification with dispersive solid phase extraction (d-SPE). Separation on Eclipse Plus C18 column was conducted in gradient of the mobile phase, 0.1% formic acid and 5 mM ammonium formate in methanol (A) and 0.1% formic acid and 5 mM ammonium formate in distilled water (B). The linearity of the matrix-matched calibrations expressed as the coefficient of determination was good with R2≥0.9900. The limit of quantifications (LOQs) ranged from 0.5 to 10 μg/kg for all analytes. Analysis of 51 agricultural soil samples taken in the Republic of Korea revealed concentrations less than 1.9 μg/kg for enrofloxacin, 75.5 μg/kg for chlortetracycline.CONCLUSION: The method was successfully applied to monitor 6 veterinary antibiotics from 51 field incurred agricultural soil samples in 17 provincial areas throughout the Republic of Korea. The developed method was simple, easy, and versatile and can be used for monitoring various veterinary antibiotics in soil.
Keywords
Agricultural soil; LC-MS/MS; Monitoring; Veterinary antibiotics;
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Times Cited By KSCI : 1  (Citation Analysis)
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