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http://dx.doi.org/10.5806/AST.2009.22.4.319

Analysis of residual neomycin in honey by LC-MS/MS  

Shim, Young-Eun (Department of Chemistry, Kyonggi University)
Jeong, Ji-Yoon (Pesticide & Veterinary Drug Residues Division, Korea Food & Drug Administration)
Myung, Seung-Woon (Department of Chemistry, Kyonggi University)
Publication Information
Analytical Science and Technology / v.22, no.4, 2009 , pp. 319-325 More about this Journal
Abstract
An effective and specific procedure for confirmation of neomycin, aminoglycoside antibiotic in honey was developed and validated. Honey was adjusted to pH 2 with 0.1M HCl and applied to weak cation-exchange SPE cartridge. Neomycin was eluted with basified methanol. Following separation by ion-pairing liquid chromatography, neomycin was analysed with positive electrospray ionization and MRM mode. Quantification was linear over the range of $5.0{\sim}250.0{\mu}g/kg$ ($r^2$ >0.9951). The precision (R.S.D.) and accuracy (as a bias) of quality control samples in honey ranged 11.5~18.7% and 10.9~20.9%, respectively. Established method can be applied to analysis of neomycin in honey.
Keywords
Honey; neomycin; LC-MS/MS;
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