• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.215-218
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• 2002

To confirm the antifungal activity to plant pathogenic fungi, Rhizoctonia solani, Rhizoctonia solani AG2-2, Fusarium oxysporium, Phytophthora dreschler, Alternaria sp. were selected. Helicosporium has the antifungal activity to Rhizoctonia solani, Rhizoctonia solani AG2-2, while Fusarium oxysporium, Phytophthora dreschler did not affected even though Alternaria made a feeble response to that antifungal compounds. From $^1H-NMR$ spectrum of antifungal compound, this compound was guessed to be a structure corresponding to cholesterol.

• Food Science and Preservation
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• v.15 no.6
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• pp.878-883
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• 2008

Prunus mume was extracted using various solvents including ethyl ether, ethyl acetate and n-butanol. The extracts were fractionated by column chromatography. Antimicrobial compounds (A, B and C) in fractions showing antimicrobial activity were purified by Sephadex LH 20 column chromatography, and identified by $^{1}H$- and $^{13}C$-NMR analysis as isoeugenol, nomilin and $\beta$-sitosterol, respectively.

• KSBB Journal
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• v.18 no.6
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• pp.490-494
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• 2003

For the production of antifungal compound, strain B-1 was used as a strong producing strain among bacteria isolated from various soil samples. The optimum medium for the production of antifungal compound was PDB (potato starch 0.4%, dextrose 2％, pH5.1). The optimum conditions for the production of antifungal compound didn't affect on the carbon and nitrogen sources. The produced compound showed broad antimicrobial activity to the tested strains such as five fungi and four bacteria. The optimum pH and temperature of the production antifungal compound were pH 5.0 and 28$^{\circ}C$, respectively. Ether extrct (1$\mu\textrm{g}$/${\mu}\ell$) of culture broth was confirmed inhibitory zone by the thin layer chromatography and plate assay. The antimicrobial compound was unstabled after heat (121$^{\circ}C$) trsatment. Strain B-1 was mass cultured in a 5-liter tormentor, containing 3 liters of PDB medium at 28$^{\circ}C$, pH 5.0, 120 (pm with aeration (1L/min).

• KSBB Journal
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• v.25 no.3
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• pp.244-250
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• 2010

This study characterized the anti-Helicobactor pylori activity of Xanthium strumarium L. extract obtained by lactic acid fermentation. The growth of the Lactobacillus strains was typically robust upon lactic acid production in monocultures containing Xanthium strumarium L. extract. Lactic acid fermentation in mixed cultures of Lactobacillus brevis KCTC 3498 and Lactobacillus casei KCTC 3109 produced higher of anti- H. pylori activity than monocultures. Concerning antioxidant activity of fermented extracts, total polyphenol contents were the highest in co-cultures of Lactobacillus brevis KCTC 3498 and Lactobacillus helveticus KCTC 3545. Electron donating ability using diphenyl picryl hydrazyl (DPPH) showed 70% scavenging in Lactobacillus casei KCTC 3109.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.3
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• pp.87-99
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• 1999

Manuka oil named New Zealand’s tea tree oil is oil-soluble and comes firom nature. Manuka oil and its extract $\alpha$-pinene, Oxy’less clear, R-limonene which is one of the component of Citron extracted from Grapefruit seed and Citrex were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion method was used to measure the antimicrobial activity. Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus was similar when the concentration of Manuka oil and $\alpha$-pinene are 10u/paper disk. However, antimi-crobial activity of Manuka oil fDr Escherichia coli, Staphylococcus aurem was better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene was low. Antimicrobial activity of Oxy’less clear is better than that of propyl para hydroxybenzoate(PPHB), R-limonene at all the concentration and is similar to that of $\alpha$-pinene. Antimicrobial activity.

• Journal of Korean Ophthalmic Optics Society
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• v.10 no.4
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• pp.261-266
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• 2005

Quaternary ammonium compounds(QACs) are some of the most commonly used antimicrobials. Antimicrobial polymers have been used as biomedical devices. In this Study, We synthesized new methacrylate monomers including quaternary ammonium moieties for preparation of contact lens, And also, we tested for antimicrobial activities against S. aureus, E. coli and P. aeruginosa. They showed much higher antimicrobial activities against all than the polymers of no including quaternary ammonium.

• Korean Journal of Plant Resources
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• v.12 no.4
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• pp.260-268
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• 1999

Crude extract of Coptis japonica root-stem was evaluated for antifungal activity against Phytophthora capsici, Fusarium oxysporum, Colletotrichum dematium, Colletotrichum truncatum, Botrytis cinerea, Botryosphaeria dothidea and Alternaria porri, and antifungal active compound from the extract was identified. In addition, the usefulness of the extract for some plants disease control was investigated. Crude extract of C. japonica root-stem exhibited antifungal activity against P. capsici, F. oxysporum, C. dematium, B. cinerea, B. dothidea and A. porri. Antifungal activity of the substance isolated from C. japonica root-stem was similar to a standard chemical berberine-Cl. Red-pepper fruit rot, sesame stem rot and welsh-onion alternaria leaf spot were effectively controlled by the crude extract of C. japonica root-stem. Phytotoxicity was not observed in the red-pepper and welsh-onion leaves and red-pepper and strawberry fruits with exogenous foliage application of the crude extract. Seeds germination and radicle growth of red-pepper and sesame were inhibited by the crude extract of C. japonica root-stem. 4.24g of yellowish compound per 100g of C. japonica root-stem was obtained. The compound was identified as berberine-Cl by HPLC.

• Food Science of Animal Resources
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• v.31 no.4
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• pp.609-616
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• 2011

In this study, a LCH1227 bacterial strain that possesses anti-listerial activity was isolated from fermented food and identified as Lactobacillus salivarius LCH1227 based on its morphological and biochemical properties, as well as its 16S rRNA gene sequences. Anti-listerial substance also inhibited the growth of various Gram-positive bacteria, such as vancomycinresistant Enterococcus faecalis, Streptococcus agalactiae, Bacillus cereus, Lactobacillus fermentum. The highest level of production of antimicrobial substances from L. salivarius LCH1227 occurred during the early stationary phase. The antilisterial activity was found to be stable over a broad range of pH values (2.0-12.0) and after heat treatment. However, it was inactivated by proteolytic enzymes, indicating its proteinaceous nature. The apparent molecular mass of the partially purified anti-listerial substance, as measured by Tricine-SDS-PAGE, was approximately 5 kDa.

• Journal of Food Hygiene and Safety
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• v.14 no.4
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• pp.422-427
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• 1999

Antimicrobial activity of Korean leek was effective on Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, E. coli O157:H7, Pseudomonas fluorescens, Pichia membranaefaciens. Extract from raw Korean leek and that from frozen one showed antimicrobial activity; in contrast, extract stored frozen condition or extract from blanched one lost antimicrobial activity. Filtration, centrifugation, and vacuum concentration did not affect antimicrobial activity of the leek, nor did the changes in storage pHs. Addition of ground leek to soy milk, soy curd, and broth for cold noodle resulted in the prolonged shelf life by significantly reducing the number of viable cells.

• Proceedings of the KAIS Fall Conference
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• 2010.11b
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• pp.1017-1020
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• 2010

최근 국산 농산물의 수요급증과 더불어 소비자들은 안전한 제품의 요구가 높아지고 있다. 이러한 요구에 맞는 제품 개발의 일환으로 미생물 제어가 된 고춧가루 포장제품의 품질향상을 위한 천연항균 물질 소재를 탐색하여 제품개발에 응용하였다. 천연물과 한방재료 중 고춧가루 제품에 적합한 소재를 스크리닝하여 항균물질을 탐색하였으며 고춧가루 제품 패킹에 응용하였다. 천연물 항균제의 개발 원칙은 산취 등 이취가 없어야 하며, 제품에 응용할 수 있는 용해성이나 믹싱의 효과를 높이도록 공정을 최적화 하였다. 또한 최종 천연물 항균제의 형태는 분말 형태로 개발하였다. 천연물에 대한 항균활성은 paper disk test를 통하여 비교하였으며, 항균력이 가장 우수한 소재는 황금추출물이었다. 또한 이들 추출물과 비타민 $B_1$ 인산염의 영향을 함께 비교하였다. 이 천연물 소재 추출물로 개발한 항균물질에 대한 효과는 일반세균, 대장균 검사를 실시하였다. 제품의 안정성 확보 및 유통기한 연장 가능성을 실험한 결과, 일반세균은 $1.0{\times}10^5$cfu/g, 대장균군은 $1.0{\times}10^2$cfu/g, 대장균은 불검출 되었다. 이들 추출물의 항균제 개발과 더불어 다양한 식품에 천연물 항균제로서의 적용 가능성을 모색하고자 하며, 안전한 천연 항균제 개발에 대한 기초 자료를 제공하는 기초 연구를 하고자 하였다.