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http://dx.doi.org/10.5851/kosfa.2011.31.4.609

Characterization of Anti-Listerial Substance Produced by Lactobacillus salivarius LCH1227  

Shin, Yu-Ri (Department of Animal Resources Science, Dankook University)
Lim, Kong-Boon (Department of Animal Resources Science, Dankook University)
Chae, Jong-Pyo (Department of Animal Resources Science, Dankook University)
Kang, Dae-Kyung (Department of Animal Resources Science, Dankook University)
Publication Information
Food Science of Animal Resources / v.31, no.4, 2011 , pp. 609-616 More about this Journal
Abstract
In this study, a LCH1227 bacterial strain that possesses anti-listerial activity was isolated from fermented food and identified as Lactobacillus salivarius LCH1227 based on its morphological and biochemical properties, as well as its 16S rRNA gene sequences. Anti-listerial substance also inhibited the growth of various Gram-positive bacteria, such as vancomycinresistant Enterococcus faecalis, Streptococcus agalactiae, Bacillus cereus, Lactobacillus fermentum. The highest level of production of antimicrobial substances from L. salivarius LCH1227 occurred during the early stationary phase. The antilisterial activity was found to be stable over a broad range of pH values (2.0-12.0) and after heat treatment. However, it was inactivated by proteolytic enzymes, indicating its proteinaceous nature. The apparent molecular mass of the partially purified anti-listerial substance, as measured by Tricine-SDS-PAGE, was approximately 5 kDa.
Keywords
anti-listerial substance; Lactobacillus salivarius; Listeria monocytogenes;
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