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Production and Chracteristics oil Antifungal agents from Bacteria  

김현수 (계명대학교 미생물학과)
육영민 ((주)계명 바이오테크)
여수환 (계명대학교 전통미생물자원개발 및 산업화연구센터)
Publication Information
KSBB Journal / v.18, no.6, 2003 , pp. 490-494 More about this Journal
Abstract
For the production of antifungal compound, strain B-1 was used as a strong producing strain among bacteria isolated from various soil samples. The optimum medium for the production of antifungal compound was PDB (potato starch 0.4%, dextrose 2％, pH5.1). The optimum conditions for the production of antifungal compound didn't affect on the carbon and nitrogen sources. The produced compound showed broad antimicrobial activity to the tested strains such as five fungi and four bacteria. The optimum pH and temperature of the production antifungal compound were pH 5.0 and 28$^{\circ}C$, respectively. Ether extrct (1$\mu\textrm{g}$/${\mu}\ell$) of culture broth was confirmed inhibitory zone by the thin layer chromatography and plate assay. The antimicrobial compound was unstabled after heat (121$^{\circ}C$) trsatment. Strain B-1 was mass cultured in a 5-liter tormentor, containing 3 liters of PDB medium at 28$^{\circ}C$, pH 5.0, 120 (pm with aeration (1L/min).
Keywords
Antifungal compound; bacteria strain B-1; optimum culture condition; mass production;
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Times Cited By KSCI : 3  (Citation Analysis)
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