• KSBB Journal
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• v.22 no.2
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• pp.78-83
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• 2007

In this study, we investigated the biological activity of antioxidant and antibacterial activity of Indigenous Plants, Jeju-Island., which, using methanol were extracted. The reducing activity on the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical and $O^{2-}$ and OH radical scavenging potential, in search for antioxidation activities of Indigenous Plants, were sequentially screened. Among the ten plant parts, Prunella vulgaris var. aleutica Fernald. flower had the highest antioxidative activity. 80% Methanol extracts of ten indigenous plants were screened for antibacterial activity 13 fish pathogenic bacteria by agar diffusion method. Among the various 80% Methanol extracts, the Prunella vulgaris var. aleutica Fernald, Gleichenia japonica Spreng, Microlepia marginata (panzer) Christ., Perilla frutescens var. japonica Hara. showed relatively strong antibacterial activities in the order.

• Journal of Life Science
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• v.14 no.3
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• pp.411-416
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• 2004

In this study, we investigated the biological activity of antioxidant and antimicrobiological effect of Chondria crassicaulis (CC), which, using methanol, dichlolometane and ethanol, were extracted and fractionated into four different types: hexane(CCMH), methanol (CCMM), butanol (CCMB), and aqueous (CCMA) partition layers. The reducing activity on the 1,I-diphenyl-2-picrylhydrazyl (DPPH) radical and $O^{2-}$ and $O_{2}$$O_{2}$radical scavenging potential, in search for antioxidation effects of CC partition layer, were sequentially screened. Among the four fractions, CCMM had the highest antioxidative activity. The antimicrobial activity was increased in proportion to its concentration by the paper disc method. Among the various solvent layers, the CCMB, CCMH and CCMM showed relatively strong antimicrobial activities in the order.

• Food Science and Preservation
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• v.5 no.3
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• pp.256-261
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• 1998

To investigate the function and effectiveness of natural antimicrobial agent(NAA), as freshness preserving material, extracted from medicinal herbs, their antimicrobial activities were tested against Bacillus cereus, Corynebacterium xerosis, Peudomonas syringae and Enterobacter aerogenes causing the postharvest decay of greenhouse produce. The ethanol extracts of Polygonum cuspidatum Sieb. et Zucc and Syzyrium aromaticum (L.) Merr. et Perry showed remarkable inhibitory effect on the growth of above-mentioned reference microorganisms when treated with mon than 500ppm. The antimicrobial activities of NAA were very stable in the wide spectrum of temperature (40$^{\circ}C$-180$^{\circ}C$) and pH(4-10).

• KSBB Journal
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• v.21 no.2
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• pp.164-169
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• 2006

In this study, we investigated the biological activity of antioxidant and antibacterial activity of Indigenous Plants, Jeju-Island., which, using water were extracted. The reducing activity on the 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical and $O^{2-}$ and OH radical scavenging potential, in search for antioxidation activities of Indigenous Plants, were sequentially screened. Among the ten plant parts, Prunella vulgaris var. aleutica Fernald. flower had the highest antioxidative activity. Hot water extracts of ten indigenous plants were screened for antibacterial activity 13 fish pathogenic bacteria by agar diffusion method. Among the various Hot water extracts, the Prunella vulgaris var. aleutica Fernald, Gleichenia japonica Spreng, Microlepia marginata(panzer) Christ., Perilla frutescens var. japonica Hara. showed relatively strong antibacterial activities in the order.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.2 s.57
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• pp.99-103
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• 2006

Natural essential oils showed anti-microbial activity on relatively broad spectrum of bacterial and fungal species. These materials had much more intensive anti-microbial activities than synthetic preservatives on C. albicans, A niger, and P. acnes, especially. In the experimental group, anti-microbial activity was order of tea tree oil (from Melaleuca alternifolia) > methylparaben > phytoncide (from Chamaecyparis obtusa). Also, natural essential oils had anti-oxidative and anti-inflammatory effects. These results suggest that natural essential oils can be useful as good cosmetic ingredients such as natural preservatives and anti-oxidants.

• Korean Journal of Microbiology
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• v.44 no.4
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• pp.339-345
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• 2008

This study was to detect MBL (metallo-${\beta}$-lactamase) among glucose non-fermenting Gram-negative bacilli isolated from clinical specimen and to search antimicrobial combination therapy against MBL producing Pseudomonas aeruginosa. Among fifty one isolates of Gram-negative bacilli with reduced imipenem susceptibility ($MIC{\ge}8{\mu}g/ml$), nine isolates have shown positive results in MBL detection test. They were seven Achromobacter xylosoxidans subsp. xylosoxidans and two P. aeruginosa. The results from EDTA-DDST coin-cided with those of PCR and nucleotide sequence analysis which showed the presence of $bla_{VIM-2}$. The combination of aztreonam (AZT) and piperacillin-tazobactarn (TZP) or AZT and amikacin (AN) screened by one disk synergy test showed no synergistic effect. Triple antibiotic combination therapy with AZT, TZP and AN, however, was shown to be effective and the most synergistic after 8 hrs of exposure. This result strongly suggest that the triple combination therapy of AZT, TZP, and AN could be useful for the treatment of infection caused by MBL producing Gram-negative bacilli.

• Restorative Dentistry and Endodontics
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• v.28 no.2
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• pp.162-168
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• 2003

이 연구는 기존의 레진 근관봉함재를 보완하여 개발한 근관봉함재(Adseal; 새로운 레진 계통의 근관봉함재)를 이미 상품화된 레진 계통의 근관봉함재(AH 26, AH Plus), 산화 아연 유지놀 계통의 근관봉함재(TubliSeal EWT, Pulpcanal sealer EWT), 수산화 칼슘 계통의 근관봉함재(Sealapex)와 비교하여 세포독성과 항균작용을 평가하고자 한다. 세포독성 실험은 L929 쥐의 섬유아세포를 사용하여 세포의 viable ratio를 계산한 후, Giemsa stain으로 염색하여 세포의 양상을 관찰하였고, 항균작용 실험은 Enterococcus faecalis Porphyromonas endodontalis, Porphyomonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum 와 Fusobacterium necrophorum를 사용하여 agar diffusion test로 평가한다. Adseal은 다른 근관봉함재에 비해 훨씬 낮은 세포독성을 보였고, AH Plus, AH 26, TubliSeal EWT, Sealapex, Pulpcanal sealer EWT의 순으로 세포독성의 정도가 높아짐을 알 수 있었다. 또한 Adseal은 Enterococcus faecalis 에서는 낮은 항균작용을 보이지만, Black-pigmented bacteria 에서는 높은 항균작용을 보이는데, 모든 근관봉함재는 서로 다른 종에 따라 어느 정도의 항균효과를 가지고 있음을 알 수 있었다.

• Research in Plant Disease
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• v.25 no.4
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• pp.179-187
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• 2019

This study was carried out to test the antimicrobial activities of nano metal hybrid materials produced by plasma technologies (radio frequency-thermal plasma system and direct current sputtering system) against microbes isolated from cucurbit (watermelon, pumpkin, and gourd) seeds. Eight different nano metal hybrid materials and four carriers were tested against five different fungal and ten different bacterial isolates in vitro. Among the tested nano metal hybrid material, Brass/CaCO₃ (1,000 ppm) exhibited 100% antimicrobial effect against all the five tested fungi. However, nano metal hybrid material Brass/CaCO₃ (1,000 ppm) inhibited only four bacterial isolates, Weissella sp., Rhodotorula mucilaginosa, Burkholderia sp., and Enterococcus sp. at 100% level, and did not inhibited other six bacterial isolates. Nano metal hybrid material graphite-nickel (G-Ni) showed 100% inhibition rate against Rhizopus stolonifer and 52.94-71.76% inhibition rate against four different fungal isolates. Nano metal hybrid material G-Ni did not show any inhibition effects against tested ten bacterial isolates. In summary, among the tested eight different nano metal hybrid materials and four carriers, Brass/CaCO₃ showed inhibition effects against five fungal isolates and four bacterial isolates, and G-Ni showed variable inhibition effects (52.94-100%) against five fungal isolates and did not show any inhibition effects against all the bacterial isolates.

• KSBB Journal
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• v.16 no.2
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• pp.123-127
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• 2001

For testing antimicrobial activity of functional plastics containing antimicrobial agent. We have used Escherichia. coli (ATCC 25922) and Staphylococcus. aureus (ATCC 6538P) with plastics which contained a Ag-hydroxyl apatite agent. In this paper, effect of antimicrobial test on plastics containing silver complex agent which accomplished by using test condition that Trypticase Soy Broth (TSB solution have diluted 500 times with 0.1 M phosphate buffer), pH7.2, Temp. $37\pm1^{circ}C$ and 120 rpm for 24 hr. the condition give the opportunity to better perform the antimicrobial active effect of individual functional plastics. As result. the test conditions were best effect of antimicrobial by using plastic contained minimum 0.5% with Ag-hydroxyl apatite agent.

• KSBB Journal
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• v.21 no.2
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• pp.129-132
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• 2006

The importance of natural preservative has increased in recent years. The natural preservatives have been used in the field of foods, cosmetics and pharmacology. In the present work Artemisia sp., well recognized for their effect of antimicrobial activity, were extracted by methanol and water sequentially for selecting only water-soluble compounds that can be used as additives in food and cosmetics. Antimicrobial activities of water extracts from stem and leaf of Artemisia princeps var. orientalis were investigated by the disc diffusion method. Two gram positive bacteria(Staphylococcus aureus and Bacillus subtilis) and three gram negative bacteria(Escherichia coil, Agrobacterium tumefaciens and Pseudomonas putida) were used for antimicrobial activity studies. The water-soluble compounds from methanol extract showed higher antimicrobial activity than only water extract to these bacteria. Comparative evaluation of water-soluble metabolite profiles with caffeic acid that is known as an antimicrobial compound from Artemisia sp. was performed by high performance liquid chromatography with photo-diode array detection.