• Title/Summary/Keyword: 품종 식별

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Use of Simple Sequence Repeat (SSR) Markers for Variety Identification of Tomato (Lycopersicon esculentum) (Simple Sequence Repeat (SSR) Marker를 이용한 토마토 품종 식별)

  • Kwon, Yong-Sham;Park, Eun-Kyung;Bae, Kyung-Mi;Yi, Seung-In;Park, Soon-Gi;Cho, Il-Ho
    • Journal of Plant Biotechnology
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    • v.33 no.4
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    • pp.289-295
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    • 2006
  • This study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for varietal identification and genetic diversity in 28 commercial tomato varieties. The relationship between marker genotypes and 28 varieties was analyzed. Of the 219 pairs of SSR primers screened against ten tomato varieties, 18 pairs were highly polymorphic with polymorphism information content (PIC) ranging from 0.467 to 0.800. Among the polymorphic loci, two to nine SSR alleles were detected for each locus with an average of 3.3 alleles per locus. Genetic distances were estimated according to Jaccard's methods based on the probability that the amplified fragment from one genotype would be present in another genotype. These varieties were categorized into cherry and classic fruit groups corresponding to varietal types and genetic distance of cluster ranging from 0.35 to 0.97. The phonogram discriminated all varieties by marker genotypes. The SSR markers proved to be useful variety identification and genetic resource analysis of tomato.

Classification of Korean Lentinula edodos Strains by Random Amplified Polymorphic DNA (RAPD) Markers (RAPD(Random Amplified Polymorphic DNA) 검정을 이용한 한국 표고균주의 계통분류)

  • Lee, Tai-Soo;Bak, Won-Chull;Kang, Ho-Duck;Kim, Se-Kwon;Byun, Byung-Ho;Yi, Chang-Keun;Lee, Won-Kyu;Min, Du-Sik
    • The Korean Journal of Mycology
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    • v.25 no.3 s.82
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    • pp.219-225
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    • 1997
  • Random Amplified Polymorphic DNA (RAPD) assay was used to identify seven typical Lentinula edodes (Berk.) Pegler strains isolated in Korea. Twenty primers from OPA-01 to OPA-20 were applied to generate the recognition of L. edodes strains. Out of 20 primers, nine primers showed efficient RAPD patterns to classify the 7 strains tested, but the rest eleven primers were not useful to be used. Even though there was no single primer that could classify all of the strains, any combination of two primers among the nine primers could identify the strains tested. Thus, RAPD assay turned out to be very precise method for classifying L. edodes strains.

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텔레바이오인식기반 반려동물 개체식별기술 표준화 동향

  • Kim, Jason
    • Review of KIISC
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    • v.30 no.4
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    • pp.35-38
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    • 2020
  • 반려견·반려묘 등 반려동물이 사람인구 대비 1/5인 수준인 1000만 개체로 급증하고, 이에 따른 팻산업이 6조원 규모로 급성장하는 추세와 함께 RFIP칩 신체내부이식하는 형태의 동물등록제 실시에 대한 부정적인 시각과 거부감으로 인하여 최근 들어 반려동물의 비문(일명 코지문)·홍채 등 바이오인식기술을 이용한 개체식별기술에 대한 니즈가 증대되고 있는 실정이다. 이에 따라 2019년 부터 반려동물에 대한 바이오인식기술을 접목한 개체식별기술을 활용하는 동물등록제 적용을 위하여 동물보호법 개정작업이 활발히 진행될 것으로 전망되며, 반려동물의 유사한 품종에 대한 개체식별 필요성에 따라 팻보험 사기예방·유기견 예방·디지털 동물병원 헬스케어서비스·팻레저·팻복지 등의 다양한 팻산업 응용분야에서 바이오인식기술을 이용한 반려동물 개체식별의 필요성은 날로 증대될 것으로 전망된다. 이에 따라 본 논문에서는 텔레바이오인식기반의 반려동물 개체식별기술에 대한 연구필요성과 함께 TTA PG505 등 국내표준화 추진현황과 ITU-T SG17 등 국제표준화 추진현황을 살펴보고자 한다.

Identification of Rice Variety Using Simple Sequence Repeat (SSR) Marker (Simple sequence repeat (SSR) marker를 이용한 벼 품종 식별)

  • Kwon, Yong-Sham;Park, Eun-Kyung;Park, Chan-Ung;Bae, Kyung-Mi;Yi, Seung-In;Cho, Il-Ho
    • Journal of Life Science
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    • v.16 no.6
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    • pp.1001-1005
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    • 2006
  • The objective of this study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for genetic diversity assessment and identification of rice varieties. The 23 primers selected from 50 SSR primers showed polymorphisms in the 21 rice varieties. The 2 to 9 SSR alleles were detected for each locus with an average of 3.00 alleles per locus. The polymorphism information content (PIC) ranged form 0.091 to 0.839. Based on band patterns, UPGMA cluster analysis was conducted. These varieties were separate into 4 groups corresponding to rice ecotype and pedigree information and genetic distance of cluster ranging from 0.59 to 0.92. The 4 SSR primer sets (RM206, RM225, RM418, RM478) selected form 23 polymorphic primers were differentiated all the rice variety from each other by markers genotypes. These markers may be used wide range of practical application in variety identification of rice.

Identification of White Hanwoo Breed Using Single Nucleotide Polymorphism Markers (단일염기다형성 마커를 이용한 백우 품종 식별 방법)

  • Kim, Seungchang;Kim, Kwanwoo;Roh, Heejong;Kim, Dongkyo;Kim, Sungwoo;Kim, Chalan;Lee, Sanghoon;Ko, Yeounggyu;Cho, Changyeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.1
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    • pp.240-246
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    • 2020
  • This study was conducted to develop specific Single Nucleotide Polymorphism (SNP) markers to identify the genetic characteristics and breed of White Hanwoo (WH) using a molecular biological method. SNP genotyping was performed with an Illumina Bovine HD 777K SNP chip using DNA extracted from 48 Hanwoo and 22 WH. The minor allele frequency (MAF) difference of each SNP was calculated and the statistical significance (P-value) of the MAF difference was calculated through Fisher's Exact test (Genotype). SNPs with 100% difference in the MAF difference were selected based on marker selection criteria. The nine SNP markers with genetic differences were selected. The selected markers have different alleles as being Hanwoo- and WH- specific. Therefore, based on these results, it can be concluded that the Hanwoo and WH varieties can be clearly distinguished by using these SNPs. So, the patent of the WH breed identification markers was registered. WH is a breed that shows the characteristics of a Korean native species that is separate from the native Hanwoo. It is expected that genetic characteristics research on the WH can be used to identify the breed and as a knowledge base for enhancing the value of breeding stock.

Construction of a DNA Profile Database for Commercial Cucumber (Cucumis sativus L.) Cultivars Using Microsatellite Marker (Microsatellite 마커를 이용한 오이 유통품종 DNA Profile Data Base 구축)

  • Kwon, Yong-Sham;Choi, Keun-Jin
    • Horticultural Science & Technology
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    • v.31 no.3
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    • pp.344-351
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    • 2013
  • Microsatellite is one of the most suitable marker for cultivar identification as it has great discrimination power for cultivars with narrow genetic variation. The polymorphism level between 358 microsatellite primer pairs and 11 commercial cucumber cultivars was investigated. Thirty-one primer pairs showed high polymorphism within cucumber cultivars with different fruit types. These markers were applied for the constructing DNA profile data base of 110 commercial cucumber cultivars through multiplex PCR and fluorescence based automatic detection system. A total of 139 polymorphic amplified fragments were obtained by using 31 microsatellite markers. The average of PIC value was 0.610 ranging from 0.253 to 0.873. One hundred and thirty nine microsatellite loci were used to calculate Jaccard's distance coefficients for UPGMA cluster analysis. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into plant shape and fruit type. Almost the cultivars were discriminated by marker genotypes. This information may be useful to compare through genetic relationship analysis between existing variety and candidate varieties in distinctive tests and protection of plant breeders' intellectual property rights through variety identification.

Construction of a Microsatellite Marker Database of Commercial Pepper Cultivars (유통 중인 고추 품종에 대한 Microsatellite 마커 Data Base 구축)

  • Kwon, Yong-Sham;Hong, Jee-Hwa;Choi, Keun-Jin
    • Horticultural Science & Technology
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    • v.31 no.5
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    • pp.580-589
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    • 2013
  • This study was carried out to evaluate the suitability of microsatellite markers for varietal identification and genetic relationship of 170 commercial pepper cultivars. The relationship between marker genotypes and 11 pepper cultivars with different morphological traits was also analyzed. Of the 302 pairs of microsatellite primers screened against 11 pepper cultivars, 24 pairs were highly polymorphic in terms of number of alleles. These markers were applied for the construction of DNA profile data base for 170 commercial pepper cultivars. A total of 164 polymorphic amplified fragments were obtained from 24 microsatellite primers. The average polymorphism information content was 0.673 ranging from 0.324 to 0.824. One hundred and sixty four microsatellite alleles were used to calculate Jaccard's distance coefficients using unweighted pair group method. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into 3 major groups corresponding to morphological traits. The phenogram discriminated all varieties by markers genotypes. These microsatellite markers will be useful as a tool for protection of plant breeders' intellectual property rights through variety identification in distinctness, uniformity and stability test.

Construction of DNA Profile Data Base of Strawberry Cultivars Using Microsatellite Markers (Microsatellite 마커를 이용한 딸기 품종의 DNA Profile Database 구축)

  • Hong, Jee-Hwa;Choi, Keun-Jin;Kwon, Yong-Sham
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.853-863
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    • 2014
  • This study was carried out to construct a DNA profile database of 100 strawberry cultivars using microsatellite markers. Two hundred seventy four microsatellite primer pairs were screened with a set of 21 strawberry cultivars with different morphological traits. Twenty five primer pairs were selected because they produced reliable and reproducible fingerprints. These primer pairs were used to develop DNA profiles of 100 strawberry cultivars. Three to thirteen alleles were detected by each marker with an average of 7.50. The average polymorphism information content varied from 0.331 to 841 (average 0.706). Cluster analysis showed that the 100 cultivars were divided into 7 major groups reflecting geographic origin and pedigree information. Moreover, most of the cultivars could be discriminated by marker genotypes. These markers will be useful as a tool for the protection of plant breeders' intellectual property rights in addition to providing the means to intervene seed disputes relating to variety authentication.

Construction of a Microsatellite DNA Profile Database for Pear Cultivars and Germplasm (배 품종 및 유전자원에 대한 Microsatellite DNA 프로파일 데이터베이스 구축)

  • Hong, Jee-Hwa;Shim, Eun-Jo;Kwon, Yong-Sham
    • Horticultural Science & Technology
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    • v.35 no.1
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    • pp.98-107
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    • 2017
  • A DNA profile database was constructed to investigate the genetic relatedness of 72 germplasm samples of Pyrus and related cultivars using microsatellite markers. Three P. pyrifolia, four P. commus, and one P. betulifolia cultivars with different morphological traits were screened using 387 pairs of microsatellite primers. A core set of 11 primer pairs was selected to obtain 133 polymorphic amplified fragments meeting three criteria: high polymorphism information contents (PIC), high repeatability, and distinct allele patterns. The number of alleles per locus ranged between 4 and 22. Average PIC was 0.743 (range: 0.557 - 0.879). Cluster analysis using the unweighted pair - group method with arithmetical average (UPGMA) separated the 72 pear cultivars and germplasm samples into four major groups: Chinese, European pears, and a cluster of 55 Asian pears that could be reclassify into two subcluster, I - $1^{st}$ and II - $2^{nd}$, according to pedigree information. Almost all of the cultivars were discriminated by 11 microsatellite marker genotypes. The microsatellite DNA profile database may be utilized as tool to verify distinctness, uniformity, and stability between candidate cultivar, and to verify in the distinctness of existing cultivars.

Establishment of Genetic Characteristics and Individual Identification System Using Microsatellite loci in Domestic Beef Cattle (초위성체 DNA표지인자를 이용한 국내 육우집단의 품종특성 및 개체식별 체계설정)

  • Kim, Sang-Wook;Jang, Hee-Kyung;Kim, Kwan-Suk;Kim, Jong-Joo;Jeon, Jin-Tae;Yoon, Du-Hak;Kang, Seong-Ho;Jung, Hyo-Il;Cheong, Il-Cheong
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.273-282
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    • 2009
  • DNA marker information is used to identify or distinguish cattle breeds or individual animal. The purpose of this study was to apply Bovine Genotypes Kit Version 1.1/2.1 to bovine DNA samples (National Institute of Animal Science) taken from Australian / American beef (n=148), Holstein beef (n=170) and Hanwoo cattle (n=177) bred in Jeongeub, Jeonbuk, Korea, so that it could distinguish Hanwoo breed. The Bovine Genotype Kits consist of 16 ISAG MS markers, which were used to build a database of genotypes in each group. Genotyping results were analyzed using MS Tool kit and Phylip program to create phylogenetic tree. The GeneClass 2.0 was used to estimate breed identification. These analyses found that this kit had 100% capacity to distinguish Hanwoo beef, 95.3% capacity to differentiate Australian / American beef and 90% capacity to identify Korean Holstein steer beef. Hence, it is expected that 16 commercial microsatellite markers is useful to categorizegenetic characteristics of Hanwoo breed and also identify Hanwoo individuals and the origin of beef. In particular, it is expected that these markers will be advantageous in discriminating domestic Holstein beef from Australian / Americanbeef.