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http://dx.doi.org/10.7235/hort.2013.13008

Construction of a DNA Profile Database for Commercial Cucumber (Cucumis sativus L.) Cultivars Using Microsatellite Marker  

Kwon, Yong-Sham (Variety Testing Division, Korea Seed & Variety Service, Ministry of Agriculture, Food and Rural Affairs)
Choi, Keun-Jin (Variety Testing Division, Korea Seed & Variety Service, Ministry of Agriculture, Food and Rural Affairs)
Publication Information
Horticultural Science & Technology / v.31, no.3, 2013 , pp. 344-351 More about this Journal
Abstract
Microsatellite is one of the most suitable marker for cultivar identification as it has great discrimination power for cultivars with narrow genetic variation. The polymorphism level between 358 microsatellite primer pairs and 11 commercial cucumber cultivars was investigated. Thirty-one primer pairs showed high polymorphism within cucumber cultivars with different fruit types. These markers were applied for the constructing DNA profile data base of 110 commercial cucumber cultivars through multiplex PCR and fluorescence based automatic detection system. A total of 139 polymorphic amplified fragments were obtained by using 31 microsatellite markers. The average of PIC value was 0.610 ranging from 0.253 to 0.873. One hundred and thirty nine microsatellite loci were used to calculate Jaccard's distance coefficients for UPGMA cluster analysis. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into plant shape and fruit type. Almost the cultivars were discriminated by marker genotypes. This information may be useful to compare through genetic relationship analysis between existing variety and candidate varieties in distinctive tests and protection of plant breeders' intellectual property rights through variety identification.
Keywords
distinctiveness; genetic marker; plant variety protection; variety identification;
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