Browse > Article
http://dx.doi.org/10.5762/KAIS.2020.21.1.240

Identification of White Hanwoo Breed Using Single Nucleotide Polymorphism Markers  

Kim, Seungchang (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Kwanwoo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Roh, Heejong (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Dongkyo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Sungwoo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Chalan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Lee, Sanghoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Ko, Yeounggyu (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Cho, Changyeon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.21, no.1, 2020 , pp. 240-246 More about this Journal
Abstract
This study was conducted to develop specific Single Nucleotide Polymorphism (SNP) markers to identify the genetic characteristics and breed of White Hanwoo (WH) using a molecular biological method. SNP genotyping was performed with an Illumina Bovine HD 777K SNP chip using DNA extracted from 48 Hanwoo and 22 WH. The minor allele frequency (MAF) difference of each SNP was calculated and the statistical significance (P-value) of the MAF difference was calculated through Fisher's Exact test (Genotype). SNPs with 100% difference in the MAF difference were selected based on marker selection criteria. The nine SNP markers with genetic differences were selected. The selected markers have different alleles as being Hanwoo- and WH- specific. Therefore, based on these results, it can be concluded that the Hanwoo and WH varieties can be clearly distinguished by using these SNPs. So, the patent of the WH breed identification markers was registered. WH is a breed that shows the characteristics of a Korean native species that is separate from the native Hanwoo. It is expected that genetic characteristics research on the WH can be used to identify the breed and as a knowledge base for enhancing the value of breeding stock.
Keywords
Genotype; Minor Allele Frequency (MAF); Single Nucleotide Polymorphism (SNP); Illumina BovineHD 777K SNP Chip; White Hanwoo;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 K. I. Kim, J. H. Lee, K. Li, Y. P. Zhang, S. S. Lee, J. Gongora, C. Moran, "Phylogenetic relationships of Asian and European pig breeds determined by mitochondrial DNA D-loop sequence polymorphism." Animal Genetics, vol. 33, pp. 19-25, 2002. DOI: https://doi.org/10.1046/j.1365-2052.2002.00784.x   DOI
2 S. J. Lai, Y. P. Liu, Y. X. Liu, X. W. Li, Y. G. Yao, "Genetic diversity and origin of Chinese cattle revealed by mtDNA D-loop sequence variation." Molecular Phylogenetics and Evolution. vol. 38(1), pp 146-154, 2006. DOI: https://doi.org/10.1016/j.ympev.2005.06.013   DOI
3 B. S. Jemaa, M. Boussaha, B. M. Mehdi, J. H. Lee, S. H. Lee, "Genome-wide insights into population structure and genetic history of tunisian local cattle using the illumina bovinesnp50 beadchip." BMC Genomics vol 16, pp 677, 2015. DOI: https://doi.org/10.1186/s12864-015-1638-6   DOI
4 J. Canon, P. Alexandrino, I. Bessa, C. Carleos, Y. Carretero, S. Dunner, N. Ferran, D. Garcia, J. Jordana, D. Laloe, A. Pereira, A. Sanchez, K. Moazami-Goudarzi, "Genetic diversity measures of local European beef cattle breeds for conservation purposes. Genetics, Selection, Evolution. vol. 33, pp 311-32, 2001. DOI: https://doi.org/10.1186/1297-9686-33-3-311   DOI
5 S. H. Sohn, C. Y. Lee, D. H. Kim, G. B. Park, J. G. Lee, C. K. Shin, H. S. Chung, S. C. Kwack, M. K, Park, M. S. Chun, C. S. Baek, Y. D. Ko, "Chromosomal Pattern and Karyotype of the Korean Native Stripped Cattle Chickso." Journal of Animal Science and Technology. vol. 42, pp 1-8. 2000.
6 S. W. Suh, C. Y. Cho, Y. S. Kim, M. J. Byun, S. B. Choi, Y. M. Cho, K. H. Bae, J. H. Kim, "Molecular Genetic considerations of Jeju Black Cattle using Microsatellite Markers." Journal of Agriculture & Life Science. vol. 49, pp 57-65. 2015. DOI: https://doi.org/10.14397/jals.2015.49.2.57
7 S. W. Kim, J. S. Choi, C. Y. Choe, D. G. Kim, Y. G. Ko, C. S. So, H. H. Seong, "Studies on the estimation of pregnancy rate of White Hanwoo (albino Korean native cattle) with sexed semen." Journal of Animal Reproduction and Biotechnology. vol. 30, pp 45-50. 2015. DOI: https://doi.org/10.12750/JET.2015.30.1.45   DOI
8 S. S. Lee, S. H. Han, .I. C. Cho, M. S. Ko, H. Y. Jung, N. Y. Kim, J. H. Kim, D. H. Yoon, "A molecular marker and genetic method for detection of albino in Bos taurus coreanae.", Korea patent 10-1190614, 2012.
9 H. Kim, C. Y. Choe, H. H. Seong, "Analysis of Growth and Hematologic Characteristics of Endangered Korean Native Cattle", Journal of Animal Reproduction and Biotechnology. vol. 30(3), pp 175-181. 2015. DOI: http://dx.doi.org/10.12750/JET.2015.30.3.175   DOI
10 S. B. Choi, C. Y. Cho, J. H. Kim, M. J. Byun, S. W. Suh, Y. G. Ko, Y. M. Cho, Y. S. Kim, "The Performance of Growth Traits in Korean Domestic Animal Genetic Resources; Chikso, Heugu, and White Hanwoo.", The Journal of The Korean Society of International Agriculture, vol. 27, pp. 398-404, 2015. DOI: https://doi.org/10.12719/ksia.2015.27.3.398   DOI
11 S. M. Schmutz, T. G. Berryere, D. C. ciobanu, A. J. Mileham, B. H. Schmidtz, M. Fredholm, "A form of albinism in cattle is caused by a tyrosinase frameshift mutation", Mammalian Genome, vol. 15, pp. 62-67, 2004. DOI: https://doi.org/10.1007/s00335-002-2249-5   DOI
12 L. F. Groeneveld, J. A. Lenstra, H. Eding, M. A. Toro, B. Scherf, D. Pilling, R. Negrini, E. K. Finlay, H. Jianlin, E. Groeneveld, S. Weigend, GLOBALDIV Consortium, "Genetic diversity in farm animals - a review", Animal Genetics, vol. 41, pp. 6-31, 2010. DOI: https://doi.org/10.1111/j.1365-2052.2010.02038.x   DOI
13 S. C. Kim, H. S. Cheong, H. D. Shin, S. S. Lee, H. J. Roh, D. Y. Jeon, C. Y. Cho, "Genetic diversity and divergence among Korean cattle breeds assessed using a BovineHD single-nucleotide polymorphism chip", Asian-Australasian Journal of Animal Science, vol. 31(11), pp. 1691-1699, 2018. DOI: https://doi.org/10.5713/ajas.17.0419   DOI
14 B. H. Schmidtz, F. C. Buchanan, Y. Plante, S. M. Schmutz, "Linkage mapping of the tyrosinase gene to bovine chromosome 29." Animal genetics vol. 32, pp 119-120. 2001. DOI: https://doi.org/10.1046/j.1365-2052.2001.0700i.x
15 S. E. Georgescu, M. A. Manea, A. Dudu, M. Costache, "Phylogenetic relationships of the Hucul horse from Romania inferred from mitochondrial D-loop variation.", Genetics and Molecular Research. vol. 10, pp 4104-4113. 2011. DOI: https://doi.org/10.4238/2011.october.31.7   DOI
16 M. E. Foreman, M. L. Lamoreux, B. Kwon, J. E. Womack, "Mapping of the bovine albino locus.", Journal of Heredity, vol. 85, pp. 318-320, 1994. DOI: https://doi.org/10.1093/oxfordjournals.jhered.a111468   DOI
17 J. H. Kim, C. Y. Cho, S. C. Kim, S. W. Kim, S. B. Choi, S. S. Lee, "Phylogenetic characterization of white hanwoo using the mitochondrial cytochrome b gene", Journal of Life Science, vol. 25(9), pp. 970-975, 2015. DOI: https://doi.org/10.5352/jls.2015.25.9.970   DOI
18 N. D. Cameron, M. J. van Eijk, B. Brugmans, J. Peleman, "Discrimination between selected lines of pigs using AFLP markers.", Heredity (Edinb), vol. 91, pp 494-501, 2003. DOI: https://doi.org/10.1038/sj.hdy.6800314   DOI
19 V. Fajardo, I. Gonzalez, I. Lopez-Calleja, I. Martin, P. E. Hernandez, T. Garcia, R. Martin, "PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus)." Journal of Agriculture and Food Chemistry, vol. 54(4), pp. 1144-1150. 2006. DOI: https://doi.org/10.1021/jf051766r   DOI
20 S. C. Kim, I. S. Jeon, S. S. Lee, C. Y. Cho, H. J. Roh, D. Y. Jeon, "Method for identification of Baekwoo breed using single nucletide polymorphism markers.", Korea patent 10-1985659, 2019.