• Journal of Life Science
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• v.29 no.4
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• pp.421-427
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• 2019

Mobilization of storage lipids is critical for the germination of oil seeds, as they supply carbon and energy until photosynthesis commences in cotyledons. In this study, we determined the levels of plant carnitine and associated changes in these levels from seed germination to cotyledon senescence. We also examined changes in the content of unsaturated fatty acids throughout seedling development. Carnitine levels peaked on day 3 at 14.5 nM in cotyledons and decreased sharply to 7.2 nM on day 4. On development day 3 carnitine levels were maintained at around 3 nM until day 7. The unsaturated fatty acid content dropped by half at the same time as carnitine peaked (day-3), and storage lipids were almost depleted by day 5. Thereafter, carnitine was hardly detected until the second stage of cotyledon senescence, at which stage the carnitine content was 6.8 nM, similar to that on day 4 at the time of fatty acid depletion in the cotyledons. Unsaturated fatty acids levels remained constant in green cotyledons but slightly increased in the senescing cotyledons. The latter can be explained by intracellular breakdown of membrane lipids. This is the first such discovery in developing cotyledons and may offer clues regarding other roles of the acetyl unit transport system in plants. The expression of BOU was closely associated with carnitine metabolism during seed germination and cotyledon development. The results provide support for the possibility of carbon re-routing during the glyoxylate cycle in the supply of energy for early germination and development.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.6C
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• pp.267-275
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• 2009

The steel pipe of steel-concrete composite piles increases the pile strength and induces the ductile failure by constraining the deformation of the inner concrete. In this research, the load-movement relations and the reinforcement effect by the outer steel pipe in the steel-concrete composite pile were analyzed by performing three-dimensional numerical analyses, which can simulate the yielding behavior of the pile material and the elasto-plastic behavior of soils. The parameters analyzed in the study include three pile materials of steel, concrete and composite, pile diameter and loading direction. As the results, the axial capacity of the composite pile was 1.9 times larger than that of the steel pipe pile and similar with that of the concrete pile. At the allowable movement criteria, the horizontal capacity of the composite pile was 1.46 times larger than that of the steel pile and 1.25 times larger than that of the concrete pile. In addition, the horizontal movement at the pile head of the composite pile was about 78% of that of the steel pile and about 53% of that of the concrete pile, which showed that the movement reduction effect of the composite pile was significant and enables the economical design of drilled shafts.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.7
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• pp.418-425
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• 2015

Anaerobic Filters (AF) packed with porous ceramic floating media were operated at different operational conditions to identify the feasibility of the renewable bioenergy, methane production from swine wastewater and to verify the suitability of effluent from anaerobic filters for the subsequent biological nitrogen and phosphorus removal. Stepwise increase in organic loading rates (OLRs) or decrease in hydraulic retention times (HRTs) with influent TCOD concentration of 14,000 mg/L were utilized at mesophilic temperature. The maximum methane productivity of 1.74 volume of $CH_4$ per volume of reactor per day (v/v-d) was achieved at an hydraulic retention time (HRT) of 0.5 day (OLR 28 g TVS/L-d). Based on the biogas production, the highest total volatile solids (TVS) removal efficiency of 63% was obtained at an HRT of 3 days (OLR 4.67 g TVS/L-d), however based on the result from the effluent total chemical oxygen demand (TCOD) analysis, the highest TCOD removal efficiency of 75% was achieved. The effluent alkalinity concentration over the range of 2,050~2,980 mg/L as $CaCO_3$ at all operational conditions, could compensate the alkalinity destruction caused by nitrification. The effluent from the anaerobic filter operated under the HRT of 2 days showed the COD/TKN ratio of 15~35 and COD/TP ratio of 38~56. Therefore effluent C/N/P ratio is able to satisfy the optimum COD/TKN ratio of greater than 8.0 and COD/TP ratio of 33 for the subsequent biological nutrient removal.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.2
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• pp.210-216
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• 2008

Leuconostoc mesenteroides SM isolated from a raw carrot was used for the fermentation of carrot juice (CJ). Lactic acid fermentation of CJ was performed at $25^{\circ}C$ for 24 hr with $0{\sim}20%$ sucrose concentration, resulting in the production of mucilage. The fermented CJ showed the complete conversion of sugar into dextran after fermentation for 24 hr in the presence of sucrose below 15% concentration. With 15% sucrose, the fermented CJ had pH 3.8, 0.9% acidity, consistency index of $3.5\;Pa{\cdot}s^n$. After cold storage for 3 weeks, fermented CJ showed slight increase in acidity and relatively constant value in pH; however, the consistency index, red color value and viable cell counts were slightly decreased for 3 weeks. The ${\beta}-carotene$ content in CJ was drastically decreased by lactic acid fermentation for 24 hr, indicating $72\;{\mu}g/mL$ as compared to $142\;{\mu}g/mL$. The fermented CJ had improved body texture, red color and stability without sedimentation.

• Journal of the Korean Society for Nondestructive Testing
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• v.28 no.5
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• pp.436-442
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• 2008

Graphite material has been recognized as a very competitive candidate for reflector, moderator, and structural material for very high temperature reactor (VHTR). Since VHTR is operated up to $900-950^{\circ}C$, small amount of impurity may accelerate the oxidation and degradation of carbon graphite, which results in increased porosity and lowered fracture toughness. In this study, ultrasonic wave propagation properties were investigated for both as-received and degradated material, and the feasibility of ultrasonic testing (UT) was estimated based on the result of ultrasonic property measurements. The ultrasonic properties of carbon graphite were half, more than 5 times, and 1/3 for velocity, attenuation, and signal-to-noise (S/N) ratio respectively. Degradation reduces the ultrasonic velocity slightly by 100 m/s, however the attenuation is about 2 times of as-receive state. The results of probability of detection (POD) estimation based on S/N ratio for side-drilled-hole (SDHs) of which depths were less than 100 mm were merely affected by oxidation and degradation. This result suggests that UT would be reliable method for nondestructive testing of carbon graphite material of which thickness is not over 100 mm. In accordance with the result produced by commercial automated ultrasonic testing (AUT) system, human error of ultrasonic testing is barely expected for the material of which thickness is not over 80 mm.

• Korean Journal of Agricultural Science
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• v.23 no.1
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• pp.13-24
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• 1996

The following results were obtained by analyzing the displacement, strain and stability of ground at the soft ground excavation using sheet pile. 1. Before setting the strut, the horizontal displacement was large on the upper part of excavated side, but after setting the strut, it showed concentrated phenomenon while being moved to go down to the excavated side. 2. After setting the strut, the displacement of sheet pile was rapidly decreased about a half compared with before setting the strut. The limitation of excavation depth was shown approximately GL-8m after setting double stair strut. 3. Maximum shear strain was gradually increased with depth of excavation, and local failure possibility due to shear deformation at the bottom of excavation was decreased by reinforcement of strut. 4. Maximum horizontal displacement of sheet pile at GL-7.5m was shown 0.2% of excavation depth in elasto-plastic method, and 0.6% in finite-element methods, and the maximum displacement was occurred around the bottom of excavation. 5. To secure the safety factor about penetration depth in the ground of modeling, D/H should be more than 0.89 in the case of one stair strut, and more than 0.77 in the case of double stair strut. 6. The relation of safety factor and D/H about the penetration depth was appeared, Fs=0.736(D/H) + 0.54 in the case of one stair strut, and Fs=0.750(D/H) + 0.62 in the case of double stair strut.

• Korean Journal of Environmental Agriculture
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• v.29 no.3
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• pp.221-226
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• 2010

Swine wastewater contains high amounts of organic matter and nutrients (nitrogen and phosphorus). The biological nitrogen removal can be achieved by nitrification and denitrification processes. Nitrification-denitrification can be performed via nitrite which is called as the short-cut process. This Short-cut process saves up to 25% of oxygen and 40% of external carbon during nitrification and denitrification. In this study, the batch tests were conducted to assess the different parameters for the nitrite sulfur utilizing denitrification, such as alkalinity, temperature, initial nitrite concentration, and dissolved oxygen. The experimental results showed that the nitrite removal efficiency of the reactor was found to be over 95% under the optimum condition ($30^{\circ}C$ and sufficient alkalinity). Autotrophic nitrate denitrification was inhibited at low alkalinity condition showing only 10% removal efficiency, while nitrite denitrification was achieved over 95%. The nitrite removal rates were found similar at both $20^{\circ}C$ and $30^{\circ}C$. In addition, nitrite removal efficiencies were inhibited by increasing oxygen concentration, but sulfate concentration increased due to sulfur oxidation under an aerobic condition. Sulfate production and alkalinity consumption were decreased with nitrite compared those with nitrate.

• Journal of Applied Biological Chemistry
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• v.60 no.4
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• pp.351-355
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• 2017

Tolaasin, peptide toxin produced by Pseudomonas tolaasii, causes a brown blotch disease on the cultivated mushrooms. Tolaasin peptides form membrane pores and disrupt cellular membrane structure. Molecular actions of tolaasin consist of the aggregation of peptide molecules, binding to the cell membrane, and formation of membrane pores. Therefore, the inhibitions of any of these actions are able to suppress the blotch disease. We have isolated and identified several tolaasin inhibitors (named tolaasin inhibitory factors, TIF) from food additives. TIFs were able to suppress the blotch-formation by the pathogen inoculated to the mushrooms. In this study, TIFs were incubated under various conditions and their activities for the inhibition of tolaasin-induced hemolytic activity were investigated. Since TIFs are unsaturated carbon compounds, they were sensitive to the air exposure and light irradiation. In the anaerobic conditions, TIFs were stable and their activities were decreased by 10% for three months. However, near 90% of TIF activity was suppressed by two weeks in the presence of air and sun light. Temperature did not show any significant effects on the activity of TIF, since storages at 5, 25, $45^{\circ}C$ did not show any difference. Therefore, for the stable storage of TIF compounds, container should be designed to be dark and air-tight.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.1
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• pp.1-7
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• 2008

When historical or cultural buildings need to be repaired or reinforced, the changes of original features should be minimized, and the strengths of structures should be improved. Among the existing methods to reinforce historical wood structures, the carbon fiber reinforcement polymer (CFRP) installation method is one of the best ones to achieve the afore-mentioned requirements. Therefore, this study aims at investigating the reinforcing effects and failure modes of timber beams reinforced with the inserted CFRP, a part of roof trusses in modern wood structures, and at providing the fundamental test data to estimate the CFRP rein-forced timber beam in the application of this reinforcing method. The primary parameters in this study were the layout and amount of CFRP. It was observed that, when $0.3{\sim}0.7%$ of CFRP were installed, the strengths of reinforced timber beams increased up to 173% compared to its original strength, but their strengthening effects were heavily influenced by the characteristics of timber such as burls. In order to improve the applicability of this strengthening method, fundamental understandings on the characteristics of wood would be necessary, and there would be in need of researches on the non-destructive test for wood structures as well.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.21 no.3
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• pp.161-168
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• 1988

The Stability of PSP extracted from the intoxicated sea mussel, Mytilus edulis was evaluated by the thange of heating conditions and pH of the PSP solution. Also the composition of the PSP extracted from the cultured sea mussel collected at Chungmu, Korea on March 12, 1986 was analyzed. The extracted PSP was stable over the range of pH 2.0 to 4.0, but it was unstable above pH 4.5. For example. the toxicity of extracted PSP of pH 3.0 was only decreased less than $20\%$ by the treatment at $121^{\circ}C$ for 15min or at 100 for 2 hours, but it was decreased more than $80\%$ by the same treatment when the pH of the PSP solution was adjusted to 6.0. The toxin was purified from the ethanolic extract of the digestive glands of the sampled sea mussel by Bio-gel P-2 and Bio-Rex 70 column chromatography. The toxic fractions obtained were analyzed by cellulose acetate membrane electrophoresis, TLC and HPLC. The compositional analytical results of the PSP, most of the toxins were certified as $GTX_{1-4}$, while the toxicity of STX was only about 1/40 of that of $GTX_s$.