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http://dx.doi.org/10.3839/jabc.2017.055

Stability increase in the activity of tolaasin inhibitors under reducing conditions  

Yun, Yeong-Bae (Department of Environmental and Biological Chemistry, Chungbuk National University)
Kim, Min-Hee (Department of Environmental and Biological Chemistry, Chungbuk National University)
Kim, Young-Kee (Department of Environmental and Biological Chemistry, Chungbuk National University)
Publication Information
Journal of Applied Biological Chemistry / v.60, no.4, 2017 , pp. 351-355 More about this Journal
Abstract
Tolaasin, peptide toxin produced by Pseudomonas tolaasii, causes a brown blotch disease on the cultivated mushrooms. Tolaasin peptides form membrane pores and disrupt cellular membrane structure. Molecular actions of tolaasin consist of the aggregation of peptide molecules, binding to the cell membrane, and formation of membrane pores. Therefore, the inhibitions of any of these actions are able to suppress the blotch disease. We have isolated and identified several tolaasin inhibitors (named tolaasin inhibitory factors, TIF) from food additives. TIFs were able to suppress the blotch-formation by the pathogen inoculated to the mushrooms. In this study, TIFs were incubated under various conditions and their activities for the inhibition of tolaasin-induced hemolytic activity were investigated. Since TIFs are unsaturated carbon compounds, they were sensitive to the air exposure and light irradiation. In the anaerobic conditions, TIFs were stable and their activities were decreased by 10% for three months. However, near 90% of TIF activity was suppressed by two weeks in the presence of air and sun light. Temperature did not show any significant effects on the activity of TIF, since storages at 5, 25, $45^{\circ}C$ did not show any difference. Therefore, for the stable storage of TIF compounds, container should be designed to be dark and air-tight.
Keywords
Cytotoxicity of tolaasin; Peptide toxin; Pore formation; Pseudomonas tolaasii; Storage conditions;
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1 Tajalipour S, Hassanzadeh N, Jolfaee HK, Heydari A, Ghasemi A (2014) Biological control of mushroom brown blotch disease using antagonistic bacteria. Biocontrol Sci Technol 24: 473-484   DOI
2 Uddin MN, Yesmin S, Khan MA, Tania M, Moonmoon M, Ahmed S (2011) Production of oyster mushrooms in different seasonal conditions of Bangladesh. J Sci Res 3: 161-167
3 van den Bogaart G, Guzman JV, Mika JT, Poolman B (2008) On the mechanism of pore formation by melittin. J Biol Chem 238: 33854-33857
4 Yun YB, Kim MH, Han JH, Kim YK (2017) Suppression of brown blotch disease by tolaasin inhibitory factors. J Appl Biol Chem 60: 179-184   DOI
5 Peng JT (1986) Resistance to disease in Agaricus bisporus (lange) Imbach. Dissertation, Department of Plant Science, University of Leeds, UK
6 Ahmad MU (2017) Fatty acids: chemistry, synthesis, and applications. Naturally occurring fatty acids: source, chemistry, and uses, 1st edn. Academic press and AOCS press, London, pp 24-71
7 Brodey CL, Rainey PB, Tester M, Johnstone K (1991) Bacterial blotch disease of the cultivated mushroom is caused by an ion channel forming lipodepsipeptide toxin. Mol Plant Microbe Interact 4: 407-411   DOI
8 Clement MV, Sivarajah S, Pervaiz S (2005) Production of intracellular superoxide mediates dithiothreitol-dependent inhibition of apoptotic cell death. Antioxid Redox Signal 7: 456-464   DOI
9 Chatterjee S, Zareena N, Gautam S, Soumyakanti A, Prasad SV, Arun S (2007) Antioxidant activity of some phenolic constituents from green pepper (Piper nigrum L.) and fresh nutmeg mace (Myristica fragrans). Food Chem 101: 515-523   DOI
10 Cho KH, Park KS, Kim YK (2000) Hemolytic properties of tolaasin causing the brown blotch disease on oyster mushroom. J Korean Soc Agric Chem Biotech 43: 190-195
11 Dolatabadian A, Jouneghani RS (2009) Impact of exogenous ascorbic acid on antioxidant activity and some physiological traits of common bean subjected to salinity stress. Not Bot Hort Agrobot Cluj 37: 165-172
12 Lee MT, Sun TL, Hung WC, Huang HW (2013) Process of inducing pores in membranes by melittin. Proc Natl Acad Sci USA 110: 14243-14248   DOI
13 Emanuele MC, Scaloni A, Lavermicocca P, Jacobellis NS, Camoni L, Di Giorgio D, Pucci P, Paci M, Segre A, Ballio A (1998) Corpeptins, new bioactive lipodepsipeptides from cultures of Pseudomonas corrugata. FEBS Lett. 433: 317-320   DOI
14 Fletcher JT, Gaze TH (2008) Mushroom: Pest and Disease Control, 2nd edn. Manson Publishing, London, pp 192
15 Gunstone FD (1984) Reaction of oxygen and unsaturated fatty acids. J Am Oil Chem Soc 61: 441-447   DOI
16 Hutchison MI, Johnstone K (1993) Evidence for the involvement of the surface active properties of the extracellular toxin tolaasin in the manifestation of brown blotch disease symptoms by Pseudomonas tolaasii on Agaricus bisporus. Physiol Mol Plant Pathol 42: 273-384
17 Kim MH, Park SW, Kim YK (2011) Bacteriophages of Pseudomonas tolaasii for the biological control of brown blotch disease. J Korean Soc Appl Biol Chem 54: 99-104
18 Li H (2010) Kinetics and mechanisms of the oxidation processes for unsaturated hydrocarbon modified scavengers. Dissertation, University of Toledo
19 Munsch P, Alatossava T (2002) Several pseudomonads, associated with cultivated mushrooms Agaricus bisporus and Pleurotus sp., are hemolytic. Microbiol Res 157: 311-315   DOI
20 Oh SJ, Kim HK, Kim HK, Fermor TR (2000) Effect of sodium hypochlorite for controlling bacterial blotch on Pleurotus ostreatus. Mycobiol 28: 123-126   DOI
21 Park SJ, Han JH, Kim YK (2016) Isolation of bacteriophage-resistant Pseudomonas tolaasii strains and their pathogenic characters. J Appl Biol Chem 59: 351-356   DOI
22 Raghuraman H, Chattopadhyay A (2007) Melittin: a membrane-active peptide with diverse functions. Biosci Rep 27: 189-223   DOI