• The Korean Journal of Mycology
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• v.44 no.3
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• pp.171-175
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• 2016

A gram-negative bacterium was isolated from spent substrates of Agaricus bisporus and showed significant antagonistic activity against Pseudomonas agarici. The bacterium was identified as Alcaligenes sp. based on cultural, biochemical, physiological characteristics and a 16S rRNA sequence analysis. The isolate is saprophytic, but not parasitic or pathogenic on cultivated mushroom, whereas it showed strong inhibitory effects against P. agarici cells in vitro. The control efficacy of Alcaligenes sp. HC12 against brown blotch of P. agarici was up to 63% on Agaricus bisporus. The suppressive bacterium may be useful for the development of biocontrol systems.

• Journal of IKEEE
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• v.23 no.2
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• pp.586-593
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• 2019

In this paper, we introduce the wood pellet electric power generation system, which is an eco - friendly solid fuel processed pure wood, which is one of the largest capacity renewable power fuels in Korea, The Ministry of Commerce, Industry and Energy notified the Ministry of Land, Infrastructure, Transport and Tourism of the Ministry of Land, Transport and Maritime Affairs of the Ministry of Land, Transport and Maritime Affairs. Derived and validated. It is confirmed that the performance of the generator and the voltage control characteristics of excitation system are good even for the change of generator fuel. It can contribute to future reference at the plant that wants to replace fossil fuels with renewable fuels.

• Journal of Mushroom
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• v.17 no.1
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• pp.19-23
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• 2019

A gram-positive bacterium was isolated from the spent substrate of Agaricus bisporus that showed a marked antagonistic activity against Pseudomonas agarici. It was identified as Bacillus safensis HC42 based on its cultural, biochemical, and physiological characteristics, and 16S rRNA sequence. B. safensis HC42 was saprophytic, but not parasitic or pathogenic, in cultivated mushrooms and showed strong inhibition of P. agarici in vitro. Moreover, it showed a control efficacy of 66 % against browning disease caused by P. agarici in Agaricus bisporus. Therefore, B. safensis HC42 may be useful in the future for the development of a biocontrol system.

• Korean journal of applied entomology
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• v.47 no.3
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• pp.273-286
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• 2008

Road-map for the environmental friendly integrated pest management(IPM) of insect pests was drawn up on the strawberry vinyl-houses of farmer's field. Major insect pests were occurred Tetranychus urticae and Aphis gossypii during the strawberry plant seeding in the vinyl house and open field. Also, same insect pests were occurred in the vinyl house during harvesting season of strawberry. For the control of T. urticae and A. gossypii, Phytoseiulus persimilis and Aphidius colemani as natural enemies were input to the vinyl house, respectively. However, because these natural enemies could not control insect pest populations, acaricide and insecticide were sprayed. Then natural enemies were input again in the vinyl house. Natural enemies could not endure the intense cold and differences of temperature and relative humidity between day and night during strawberry harvesting season. So, their behavior and control activity of pests were more decrease than pests. Firstly, natural enemies are input in the vinyl house during the early breeding season of strawberry, secondly, acaricides and insecticide are sprayed for the control of mites and aphids, respectively, during the middle breeding season in the hard winter. Finally, natural enemies are re-input in the vinyl house during the middle and late breeding season.

• 한국지형공간정보학회:학술대회논문집
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• 2004.10a
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• pp.141-146
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• 2004

최근 들어 국내에서도 홍수에 의한 재산과 인명피해를 최소화하기 위해 지역특성에 알맞은 홍수지도를 제작 중에 있으며, 주로 LiDAR를 이용하여 홍수지도 지형자료를 구축하고 있다. 그러나 이에 많은 시간과 비용이 소요되기 때문에 이미 전국적으로 구축된 수치지형도를 이용한 홍수지도 지형자료 구축의 가능성과 타당성을 검증의 필요성이 제기되고 있다. 이에 따라 본 연구에서는 수치지형도를 이용한 홍수지도제작시 가장 핵심이 되는 정확한 지형자료구축을 위해서 Breakline처리기법과 하천종횡단자료 연계방안을 중심으로 구리시 일부지역을 대상으로 DEM을 시범제작 하였다. 수치지형도와 LiDAR를 이용하여 구축한 DEM을 검수점을 통한 RMSE오차를 이용하여 수직위치정확도를 비교분석하였다. 분석을 위하여 선행연구(홍수지도제작, 2002)에서 검수점으로 사용한 9점과 제방부분의 실측한 9점을 본 연구의 검수점으로 사용하였다. 비교 결과 RMSE가 수치지형도 DEM의 경우 ${\pm}2.658m$이고, LiDAR DEM의 경우 ${\pm}3.430m$로 나타났다. 두 자료 모두 알려진 오차범위보다 훨씬 높은 오차가 발생함을 알 수 있었다. 향후 수치지형도를 이용한 효율적인 홍수지도 지형자료 구축을 위해서는 수치지형도의 신속하고 지속적인 갱신이 필요할 것이며, 현재 도시지역에만 구축되어 있는 1:1,000 수치지형도를 주변 하천지역까지 확대해서 구축해야 할 필요성이 있을 것으로 사료된다. 아울러 도입초기단계의 LiDAR 측량기술의 보급과 관련모델의 기술의 발달로 인하여 정확도 높은 DEM이 요구된다면 고밀도의 방대한 양의 LiDAR 자료로 DEM을 구축하는 것이 바람직하다고 사료된다.및 이용대책을 수립하는 데 활용될 수 있다., $1,000-2,000{\mu}g\;g^{-1}$), 나머지 177종 식물은 살초활성을 나타내지 않았다. 높은 살초활성을 나타낸 식물로부터 분리되는 천연활성물질들은 향후 새로운 제초제 개발을 위한 모화합물 제공, 상호대립억제 작용성을 갖는 작물 품종 개발 혹은 개량에의 활용, 그리고 친환경 유기농업용 잡초방제에의 활용이 기대된다.내(皮膚上皮組織內)의 Langerhans cell내(內)의 Birbeck granule에 비(比)해 수적(數的)으로 현저히 감소(減少)되어 있었다. 그러나 Thy-1 양성(陽性) dendritic cell에서 볼 수 있는 dense-core 과립(顆粒)은 별변화(別變化)없이 쉽게 관찰(觀察)될 수 있었다. 조직배양(組織培養)을 한 견(犬)의 keratinocyte에 대(對)해 사람 pemphigus vulgaris의 항체(抗體)로 반응(反應)시킨 후 protein-A gold(15 nm)로 표식(標識)시킨 바 제일 바깥 상층(上層)의 keratinocyte에 있어서 세포막표면(細胞膜表面)을 따라 표식(標識)되어 세포막항원(細胞膜抗元)을 나타내었으며, 이와 같은 소견(所見)으로 미루어 정상피부(正常皮膚) 중층편평상피세포(重層扁平上皮細胞)에서도 동일(同一)한 소견(所見)을 관찰(觀察)할 수 있다고 본다.al remnants, Resorption of fetus로 관찰된 것이다. Fetal death는 수정후 $14{\sim}18$일까지의 사망으로써 Maceration of fetus로 관찰되는 것이다. 통계학적 분석은 각 Group의 착상 을과 자궁 내 사망 율을 산출할 때에는 각 임신마우스에 따라 발생빈도가 크

• Journal of the Korean Recycled Construction Resources Institute
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• v.11 no.2
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• pp.120-129
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• 2023

Marine structures are subject to damage not only from sea salt but also from the adhesion of marine microorganisms and suspended particles, which cause additional damages. In order to prevent this, periodic coating is employed in the case of vessels to maintain the necessary performance. However, it is true that periodic coating is difficult for concrete or steel support structures, and there is a risk of marine environmental pollution. In this study, authors developed an anti-fouling coating agent using eco-friendly materials that possess hydrophilic cellulose nanofibers and AKD(alkyl ketene dimer). To achieve a homogeneous mixture, the content of cellulose nanofibers was fixed at 1 %, and AKD, distilled water, and waste glass were mixed using a digital mixer and homogenizer. The contact angle of the prepared coated surface was observed to be over 130°, indicating sufficient performance even in a water droplet flow test with a 15° slope, suggesting self-cleaning capability. Furthermore, through the analysis of viscosity characteristics at different temperatures, it was confirmed that the application is feasible at room temperature. Microstructure analysis also verified that the coating agent is uniformly applied to the concrete surface.

• Korean journal of applied entomology
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• v.62 no.3
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• pp.193-205
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• 2023

Botanical insecticides derived from plant extracts exhibit repellent, antifeedant and enzyme-inhibiting activities against insect pests. Among such pests, phytophagous mites are major threats to horticultural crops. Botanical extracts derived from neem, chrysanthemum, and shrubby sophora are employed as field acaricides. These botanical extracts have low toxicities against natural enemies of the insect pests and, thus, are valuable in pest management. This review focuses on the potential for botanical extracts in the controls of mites, with comparisons of the spectrum of activity, the lethal dose and times and their mode of action. This information will enable better formulation of botanical extracts in integrated mite control.

• Korean Journal of Construction Engineering and Management
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• v.16 no.6
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• pp.136-143
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• 2015

Snow-melting system has been applied not only to roads for car traffic but also to pavement for the pedestrians safety reason in some of the developed countries such as USA and Canada based on countermeasures against Natural Disasters Act revised in 2000. Even though this system was introduced in korea in 2006 and has been partly applied to car traffic roads, there is few places that the system has been applied. Therefore, in this research a snow-melting system with a block-type to cover a pavement that efficiently transfers heat form heat rays to the top of a pavement and protects the heat rays. A quality check showed that compression and bending strength was improved approximately 5 times stronger and 7 to 10 times more absorption rate than the KS(Korea Industrial Standard) requirement. Moreover, only 10 minute was required to increase temperature above zero with a block-type snow-melting system whereas approximately 180 minute was spent with the existing system. This research is expected to contribute to environmental issues and reduce accidents on a slippery road.

• Research in Plant Disease
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• v.23 no.3
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• pp.268-277
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• 2017

Fusarium head bight (FHB) is a devastating disease on major cereal crops worldwide which causes primarily by Fusarium graminearum. Synthetic fungicides are generally used in conventional agriculture to control FHB. Their prolonged usage has led to environmental issues and human health problems. This has prompted interest in developing environmentally friendly biofungicides, including botanical fungicides. In this study, a total 100 plant extracts were tested for antifungal activity against F. graminearum. The crude extract of Pterocarpus santalinus heartwood showed the strongest antifungal activity and contained two antifungal metabolites which were identified as ${\alpha}$-cedrol and widdrol by GC-MS analysis. ${\alpha}$-Cedrol and widdrol isolated from P. santalinus heartwood extract had 31.25 mg/l and 125 mg/l of minimal inhibitory concentration against the spore germination of F. graminearum, and also showed broad spectrum antifungal activities against various plant pathogens. In addition, the wettable powder type formulation of heartwood extract of P. santalinus decreased FHB incidence in dose-dependent manner and suppressed the development of FHB with control values of 87.2% at 250-fold dilution, similar to that of chemical fungicide (92.6% at 2,000-fold dilution). This study suggests that the heartwood extract of P. santalinus could be used as an effective biofungicide for the control of FHB.

• Korean Journal of Microbiology
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• v.45 no.2
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• pp.228-231
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• 2009

We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.