The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Antagonistic Effects of the Bacterium Alcaligenes sp. HC12 on Browning Disease Caused by Pseudomonas agarici

버섯 세균성회색무늬병균(Pseudomonas agarici)에 대한 Alcaligenes sp. HC12의 항균활성

  • Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Moon, Ji-Won (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cheong, Jong-Chun (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA)
  • 이찬중 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 문지원 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2016.08.02
  • Accepted : 2016.09.22
  • Published : 2016.09.30
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Abstract

A gram-negative bacterium was isolated from spent substrates of Agaricus bisporus and showed significant antagonistic activity against Pseudomonas agarici. The bacterium was identified as Alcaligenes sp. based on cultural, biochemical, physiological characteristics and a 16S rRNA sequence analysis. The isolate is saprophytic, but not parasitic or pathogenic on cultivated mushroom, whereas it showed strong inhibitory effects against P. agarici cells in vitro. The control efficacy of Alcaligenes sp. HC12 against brown blotch of P. agarici was up to 63% on Agaricus bisporus. The suppressive bacterium may be useful for the development of biocontrol systems.

Pseudomonas agarici에 의해 발생하는 세균성회색무늬병은 양송이 재배에서 문제가 되는 대표적인 병해이다. 본 연구에서는 세균성회색무늬병의 생물학적 방제법에 이용할 수 있는 길항미생물의 항균활성과 선발된 길항미생물에 대해 폿트 수준의 생물검정 실험을 실시하였다. 재배중인 양송이 배지에서 세균성회색무늬병 병원균을 강하게 억제하는 길항세균 HC12를 선발하였으며, 생리 생화학적 실험과 유전적 실험결과 HC12균주는 Alcaligenes sp.로 동정되었다. Alcaligenes sp. HC12를 양송이에 처리한 결과 63%의 방제효과를 보였다. 따라서 Alcaligenes sp. HC12가 양송이버섯 세균성회색무늬병 방제를 위해 합성농약을 대체할 수 있는 친환경 방제제가 될 수 있을 것으로 생각된다.

Keywords

References

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