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http://dx.doi.org/10.14480/JM.2019.17.1.19

Antagonistic Effect of Bacillus safensis HC42 on Brown Blotch Mushroom Disease Caused by Pseudomonas agarici  

Lee, Chan-Jung (Mushroom Research Division, NIHHS, RDA)
Lee, Eun-Ji (Mushroom Research Division, NIHHS, RDA)
Park, Hae-Sung (Mushroom Research Division, NIHHS, RDA)
Kong, Won-Sik (Mushroom Research Division, NIHHS, RDA)
Publication Information
Journal of Mushroom / v.17, no.1, 2019 , pp. 19-23 More about this Journal
Abstract
A gram-positive bacterium was isolated from the spent substrate of Agaricus bisporus that showed a marked antagonistic activity against Pseudomonas agarici. It was identified as Bacillus safensis HC42 based on its cultural, biochemical, and physiological characteristics, and 16S rRNA sequence. B. safensis HC42 was saprophytic, but not parasitic or pathogenic, in cultivated mushrooms and showed strong inhibition of P. agarici in vitro. Moreover, it showed a control efficacy of 66 % against browning disease caused by P. agarici in Agaricus bisporus. Therefore, B. safensis HC42 may be useful in the future for the development of a biocontrol system.
Keywords
Agaricus bisporus; Bacillus safensis; Control efficacy; Mushrooms; Pseudomonas agarici;
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1 Lee CJ, Yoo YM, Han JY, Jhune CS, Cheong JC, Moon JW, Suh JS, Han HS, Cha JS. 2013. Isolation of the bacterium Pseudomonas sp. HC1 effective in inactivation of tolaasin produced by Pseudomonas tolaasii. Kor J Mycol 41: 248-254.   DOI
2 Lee CJ, Yoo YM, Han JY, Jhune CS, Cheong JC, Moon JW, Suh JS, Han HS, Cha JS. 2014. Isolation of the bacterium Pseudomonas azotoformans HC5 effective in antagonistic of brown blotch disease caused by Pseudomonas tolaasii. Kor J Mycol 42:219-224.   DOI
3 Nair NG, Fahy PC. 1972. Bacteria antagonistic to Pseudomonas tolaasii and their control of brown blotch of the cultivated mushroom Agaricus bisporus. J Appl Bacteriol 35: 439-442.   DOI
4 Nutkins JC, Mortishire-Smith RJ, Packman LC, Brodey CL, Rainey PB, Johnstone K, Williams DH. 1991. Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen Pseudomonas tolaasii Paine. J Am Chem Soc 113:2621-2627.   DOI
5 Palleroni NJ. Genus. Pseudomonas. 1984. In: Bergey's manual of systematic bacteriology. Vol. I, Ed. by N. R. Krieg and J. G. Hotr, P. 141-219. Williams and Wilkins, Baltmore.
6 Park BS, Cho NC, Chun UH. 1992. Identification of Pseudomonas fluorescens antagonistic to Pseudomonas tolaasii and its cultivation. Kor J Biotechnol Bioeng 7: 296-301.
7 Paine SG. 1919. Studies in bacteriosis II. A brown blotch disease of cultivated mushrooms. Ann Appl biol 5:206-219.   DOI
8 Scherwinski K, Grosch R, Berg G. 2008. Effect of bacterial antagonists on lettuce: active biocontrol of Rhizoctonia solani and negligible, short-term effects on nontarget microorganisms. FEMS Microbiol Ecol 64: 106-116.   DOI
9 Stainer RY, Palleroni NJ, Doudoroff M. 1966. The aerobic pseudomonads: a taxonomic study. J Gener Microbiol 43:159-271.   DOI
10 Saitou N, Nei M, 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biol Evol 4:406-425.
11 Thompson JD, Higgins DG, Gibson TJ. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighing position-specific gap penalties and weight matrix choice. Nucleic Acids Res 34-637.
12 Tolaas AG. 1915. A Bacterial disease of cultivated mushrooms. Phytopathology 5:51-54
13 Wells JM, Sapers GM, Fett WF, Butterfield JE, Jones JB, Bouzar H, Miller FC. 1996. Postharvest discolorization of the cultivated mushroom Agaricus bisporus caused by Pseudomonas tolaasii, P. 'reactans', and P. 'gingeri'. Phytopathology 86:1098-1104.   DOI
14 Wong WC, Fletche, JT, Unsworth BA, Preece TF. 1982. A note on ginger blotch, a new bacterial disease of the cultivated mushroom, Agaricus bisporus. J Appl Bacteriol 52:43-48.   DOI
15 Young JM. 1970. Drippy gill: a bacterial disease of cultivated mushrooms caused by Pseudomonas agarici n. sp. N Z J Agr Res 13:977-990.   DOI
16 Geels FP, Hesen LPW, Van Griensven LJLD. 1994. Brown discoloration of mushrooms caused by Pseudomonas agarici. J Phytopathol 140:249-59.   DOI
17 Bessette AE, Kerrigan RW, Jordan DC. 1985. Yellow blotch of Pleurotus ostreatus. Appl Environ Microbiol 50: 1535-1537.   DOI
18 Cutri SS, Macauley BJ, Roberts WP. 1984. Characteristics of pathogenic non-fluorescent (smooth) and nonpathogenic fluorescent (rough) forms of Pseudomonas tolaasii and Pseudomonas 'gingeri'. J Appl Bacteriol 57:291-298.   DOI
19 Fermor TR. 1986. Bacterial diseases of edible mushrooms and their control. In: Proceedings of International Symposium on Scientific and Technical Aspects of Cultivating Edible Fungi. Pennsylvania State University, University Park, PA, USA, 361-370.
20 Gill WM. 1995. Bzcterial disease of Agaricus mushrooms. Report. Tottori Mycological Institute. 33:34-35.
21 Jukes TH, Cantor CR. 1969. Evolution of protein molecules, pp.21-132. In: H. N. Munro(de.), Mammalian Protein Metabolism. Academic Press, N. Y.
22 Kim IG, Whang KS. 2002. The observation and a quantitative evaluation of viable but non-culturable bacteria in potable groundwater using epifluorescence microscopy. The Kor J Microbiol 38:180-185.
23 Lee HI, Cha JS. 1998. Cloning of a DNA Fragment Specific to Pseudomonas tolaasii Causing Bacterial Brown Blotch Disease of Oyster Mushroom (Pleurotus ostreatus). Kor J Plant Pathol 14:177-183.
24 Lee CJ, Moon JW, Cheong JC, Kong WS. 2016. Antagonistic effects of the bacterium Alcaligenes sp. HC12 on browning disease caused by Pseudomonas agarici. Kor J Mycol 44: 171-175.   DOI