• Applied Microscopy
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• v.35 no.3
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• pp.129-134
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• 2005

The cytotoxicity that chemical disinfectants of K products and natural disinfectants of naringin & chitosan well-known preservative, have on endothelium and epithelium of rabbit's cornea were obseved by scanning electron microscope. The main component of naringin is grapefruit seed extract, which is one of the flavonoid widely recognized as natural antioxidants and used as preserved food and cosmetics. The chitosan also wildly distributed epithelium of crustacea, epidermis of insects, mould, fungi and so on. It is no harm of cytotoxicity of human body and recognized of antibacterial for various of bacteria. This study was performed to examine the cytotoxicity of natural and chemical preservative.

• Food Industry And Nutrition
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• v.8 no.3
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• pp.9-17
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• 2003

숙성 기간(1, 3 및 5년)이 다른 멸치 액젓으로부터 추출한 biocompound는 정제과정에서 숙성 1년의 멸치 액젓은 3개, 숙성 3년의 멸치 액젓은 4개, 숙성 5년의 멸치 액젓은 5개의 Peak를 각각 나타내었다. 숙성 기간이 길수록 멸치 액젓의 biocompound는 저분자화 었으며, gel chromatography상의 후반부에 나타났다. 숙성 기간이 다른 멸치 액젓으로부터 추출한 biocompound들의 생리활성(항산화, 항암, ACE 저해활성 )은 숙성 기간이 길수록 증가하였다 특히 항산화 및 항암활성은 $IC_{50}$/이 각각 34 및 44 $\mu\textrm{g}$/mL인 3년 숙성 peak 3이 제일 높았으나, ACE 저해 활성은 $IC_{50}$/이 32$\mu\textrm{g}$/mL인 1년 숙성 peak 3이 제일 높았다. 천연 숙성 멸치 액젓의 저분자 peptide는 뛰어난 생리활성을 갖고 있음이 밝혀졌으나 구조 분석 등의 추가 연구가 필요하다고 본다. 5년 숙성 멸치 액젓 분획 중 항산화활성이 높은 1-A-II-d와 1-A-III을 sequence analyzer로 분석한 결과 각각 glutamic acid및 lysine의 단일 아미노산으로 나타났다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1594-1600
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• 2004

Effects of mistletoe (Viscum album) extract and pueraria (Pueraria radix) extract on cellular NF-$textsc{k}$B activity were evaluated in human malignant keratinocytes (SCC-13) to elucidate the possible correlation of NF-$textsc{k}$B with antioxidant activity. The antioxidant activities of these natural extracts were examined in four different evaluation methods, i.e., lipid peroxidation value (POV) evaluation test, I,l-diphenyl-2-picrylhydrazyl radical (DPPH), nitric oxide (NO) scavenging test, and reducing power assay. Pueraria extract (0.5 mg) and mistletoe extract (5 mg) downregulated the cellular NF-$textsc{k}$B activation up to 35% and 10% compared to the control, respectively, although their effects were lower than the known NF-$textsc{k}$B downregulator, vitamin C (8.8 mg, 53%) in a cell-based NF-$textsc{k}$B activity assay system. In the POV test, relative antioxidant activities of mistletoe extract (86%) and pueraria extract (75%) were significantly higher than the known antioxidant, vitamin C (48%) at the same concentration (10 mg) and the degree of activity increased in a dose-dependent manner. Pueraria extract showed more potential radical scavenging activities than those of mistletoe extract evaluated in both DPPH and NO test. Especially, the NO radical scavenging activity of pueraria extract ($SC_{50}$/, 88 $\mu$g) was comparable to that of vitamin C ($SC_{50}$/, 77 $\mu$g). Even pueraria extract possessed a much less reducing power compared to vitamin C, it also revealed higher reducing power than that of mistletoe extract. These results indicate that mistletoe extract and pueraria extract may serve as an useful natural antioxidant agents, and led to suggest the hypothesis that compounds having an antioxidant activity, i.e., radical scavenging activity or reducing power may be correlated with the downregulation of NF-$textsc{k}$B activation in human keratinocytes.

• Journal of Food Hygiene and Safety
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• v.28 no.4
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• pp.337-341
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• 2013

After it was known that an antioxidant function was associated with a reducing risk of cancer and cardiovascular disease, a large number of plant have been investigated for their antioxidant activity. However, lacking of the extensive antioxidant data in vegetables, a few natural antioxidant was used in the food industry. Therefore, this study was carried out to provide some scientific antioxidant activity data in the various vegetables. The samples (n = 275) including 24 kinds of vegetables were examined for antioxidant activity. Total Phenol Contents (TPCs) were measured colorimetric determination method using the Folin-Ciocalteu reagent by gallic acid as the control group. DPPH radical scavenging activity were also investigated using calculation of $IC_{50}$(concentration of extracted needed to inhibit 50% of the DPPH radical). The contents of TPCs were $0.32{\sim}49.26{\mu}g/kg$ and DPPH radical scavenging activity ($CI_{50}$) was 0.0~2.43 mg/mL in vegetables. The best antioxidant effect was shown in Garlic for both antioxidant assay methods.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.2
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• pp.207-210
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• 2003

This study was designed to investigate the antioxidative effect of glasswort (Salicornia herbacea L.) grown in closed sea beach. To compare antioxidant effects of glasswort plant parts, dried ground leaves, stems, and roots of glasswort were exploited for investigation of antioxidant activity Antioxidant activity for the samples was investigated by Rancimat and TBA method. The oxidative stability determined by the Rancimat technique showed a variation in antioxidative index (AL) between the different plant parts, ranging from 1.0 to 3.6. Oxidative stability from ground stem sample of glasswort harvested in the closed sea beach was the highest, and followed by leaf and root. Stem had higher oxidative stability than ascorbic acid. Antioxidatiue effects of glasswort were increased in dose-dependent manners, and antioxidant activity by addition of glasswort with same amount to 4% oil was similar to the activity of ascorbic acid. Specially, 8~10% glasswort addition to oil showed similar antioxidative effect to 1% $\alpha$-tocopherol.

• Korean Chemical Engineering Research
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• v.54 no.3
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• pp.310-314
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• 2016

The search for natural antioxidants as alternatives to synthetic products is growing. Microalgae have emerged as a source of natural antioxidants with significant and diverse health-promoting properties. In this study, the effects of hot-water extraction conditions on total polyphenol compounds (TPC) production were investigated for lipid extracted microalgae (LEA). In order to enhance the polyphenol productivity, the extraction variables including solvents, temperature, time and ethanol concentration were optimized. The results showed hot-water extraction provided a higher extraction efficiency than the organic solvents and extraction at high temperatures showed a better extraction efficiency. While hot-water extract showed a higher extraction efficiency compared to 98% ethanol extraction, the mixture of water and ethanol (40:60 v/v) showed the highest production of polyphenols. The maximum polyphenols of 3.35 mg GAE (gallic acid equivalent)/g DM were obtained at the optimized extraction time of 10 min, $100^{\circ}C$ and 40% ethanol, respectively. Although Tetraselmis KCTC 12236BP was preprocessed by hexane to remove lipid for bio-diesel production, the results showed LEA contains relatively high level of polyphenols compared to untreated microalgae which can be used in the production of value-added materials. The predictions obtained from the developed Peleg's model were compared with the experimental data under the same operating conditions. The predicted and experimental data were consistent, indicating the reliability of the model.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.73-78
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• 2005

This work was carried out to investigate the antioxidative effects of Acanthopnax sessiliflorus from Jeongseon County for the purpose of development of a novel antioxidant from natural products. The antioxidant activity was determined by using electron paramagnetic resonance (EPR), not measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) which have bun used for antioxidant activity of natural sources. Although DPPH radical scavenging activity assay have been generally used for antioxidant activity, this assay is nut appropriated for determinating which radical is scavenged by extracts from natural products. Using EPR, we determinated whether A. sessiliflorus extracts from Jeongseon County scavenge specific radicals or not. On experiment of scavenging superoxide anion radical, hydroxyl radical, nitrogen dioxide and peroxinitrite. Extracts from A. sessiliflorus showed high antioxidant activities to reactive oxygen and nitrogen species. These result suggest that extracts from A. sessiliflorus act as an antioxidant by scavenging reactive oxygen and nitrogen species and used as new cosmetic ingredients for anti-oxidative stress in skin.

• Journal of agriculture & life science
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• v.53 no.3
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• pp.41-49
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• 2019

Consumers are interested in natural ingredient that replace synthetic antioxidants in meat products. This study was carried out to investigate the effects of astaxanthin (AX) on the oxidative stability and quality characteristics of emulsified sausages during cold storage. Emulsified sausages were prepared as follows: manufactured without AX and BHT (Control), added with 500 mg/kg of BHT (BHT), and added with 80 mg/kg of AX (AX). Addition of AX showed no significant difference in pH, emulsion stability and cooking yield of emulsified sausages (p>0.05). However, the initial color retentivity was the same as that of synthetic antioxidant such as BHT treatment, and the redness was higher when AX treatment was added (p<0.05). The lipid oxidation showed the lowest value in the BHT treatment at the end of storage and the AX treatment also was significant lower than that of control (p<0.05). Hardness was lower in the all treatments at the end of storage than in the control (p<0.05). Therefore, astaxanthin can be used as a color enhancing agent for meat products as well as an natural antioxidant in replacing of BHT which is as synthetic antioxidant.

• Korean Journal of Food Science and Technology
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• v.35 no.6
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• pp.1216-1220
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• 2003

The antioxidative activities of methanol extracts of chestnut flower (Castanea Crenata Flos.) were determinated in vitro using an experimental model system. Solid yield of chestnut flower extracts was 6.26% and total phenolic acid accounted for about 20% of the crude extract. The DPPH radical scavenging activity of methanol extracts prepared from chestnut flower was 17.22%. Although the DPPH radical scavenging activity of chestnut flower extracts was lower than that of other antioxidants, chestnut flower extracts showed continuous activity by time course. The SOD-like activities of methanol extracts prepared from chestnut flower were 65.10%, 95.70% in BHT, 93.29% in quercetin, and 30.30% in ascorbic acid. Chestnut flower extracts showed 51.45% inhibitory effect on peroxidation of egg yolk lecithin.

• Food Science and Preservation
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• v.7 no.4
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• pp.373-379
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• 2000

The Ulmi corex extract was prepared using various solvents to investigate the availability as a natural antioxidant. The extracts were added to lard emulsion and the antioxidant activities were compared. The extract that had a greater antioxidant activity was fractionized. Then the antioxidant activity and substrate specificity of the fraction were examined and optimum concentration of addition was determined. To observe the antioxidative effect of the fraction in vivo, an inhibition rate of lipid peroxidation from which might be derived was measured using a microsome in rat's liver. Among the extracts of Ulmi cortex, the extract from water had the best antioxidant activity, and the addition of 0.05% (w/w) of ethyl acetate fraction showed similar antioxidant activity to a synthetic antioxidant, butylated hydroxyanisole(BHA). Ethyl acetate fraction (0.05%, w/w) also presented the antioxidative effect in lard, soybean oil, palm oil, and com oil. The inhibition of lipid peroxidation in liver microsome showed feater in the ethyl acetate fraction than caffeic acid in both nonenzymatic peroxidation (Fe$\^$++/ascorbate system) and enzymatic peroxidation (Fe$\^$++/-ADP/NaDPH system).