• Korean Journal of Microbiology
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• v.49 no.1
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• pp.17-23
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• 2013

Candida biofilms are organized microbial communities growing on the surfaces of host tissues or indwelling medical devices, and the biofilms show enhanced resistance against the conventional antifungal agents. The roots of Coptidis chinensis have been widely used for medicinal purposes in East Asia. The present study was aimed to assess the effect of C. chinensis aqueous extract upon preformed biofilms of 10 clinical Candida albicans isolates and the antifungal activities which contribute to inhibit the C. albicans biofilm formation. Its effect on preformed biofilms was judged using XTT [2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)] reduction assay, and metabolic activity of all tested strains was reduced significantly ($57.3{\pm}14.7%$) at $98{\mu}g/ml$ of the C. chinensis extract. The extract damaged the cell membrane of C. albicans which was analyzed by fluorescein diacetate and propidium iodide staining. The anticandidal activity was fungicidal, and the extract obstructed the adhesion of C. albicans biofilms to polystyrene surfaces, arrested C. albicans cells at $G_o/G_1$ as well, and reduced the growth of biofilms or budding yeasts finally. The data suggest that C. chinensis has multiple antifungal effects on target fungi resulting in preventing the formation of biofilms. Therefore, C. chinensis holds great promise for exploring antifungal agents from natural products in treating and eliminating biofilm-associated Candida infection.

• Polymer(Korea)
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• v.31 no.1
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• pp.14-19
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• 2007

Demineralized bone particle (DBP) has been used as one of the powerful inducers of bone and cartilage tissue specialization. In this study, we fabricated DBP/PLGA scaffold for tissue engineered disc regeneration. We manufactured dual-structured scaffold to compose inner cylinder and outer doughnut similar to nature disc tissue. The DBP/PLGA scaffold was characterized by porosity, wettability, and water uptake ability. We isolated and cultured nucleus pulposus (NP) and annulus fibrosus (AF) cells from rabbit intervertebral disc. We seeded NP cells into the inner core of the hybrid scaffold and AF cells into the outer portion of it. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl) -2,5- diphenyltetrazolium -bromide (MTT) test. PLGA and PLGA/DBP scaffolds were implanted in subcutaneous of athymic nude mouse to observe the formation of disc-like tissue in vivo. And then we observed change of morphology and hematoxylin and eosin (H&E). Formation of disc-like tissue was better DBP/PLGA hybrid scaffold than control. Specially, we confirmed that scaffold impregnated 20 and 40% DBP affected to proliferation of disc cell and formation of disc-like tissue.

• Journal of the Korean Society of Clothing and Textiles
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• v.21 no.5
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• pp.911-921
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• 1997

Recently the interest in natural dyeing has been increased because of the color clarity, natural grace and reactionism in fashion. Indigo dyeing, safflower dyeing and Gal-ott in Cheju-Do become generally known, so the study about the natural dyeing is continued with national intrust and support. In this study, 1 used artemisia for various dyeing tests because we can get it easily. 1 tested the dyeability in wool as well as cotton and silk with wormwood in natural dyeing material. I also dyed nylon with the same material for the comparison of the molecular structure. The mercerization and the chitosan treatment were done in cotton to improve the low dyeability in the natural dyeing. The result of this study are as follows; We have to dye repeatedly to get deep color in natural dyeing, and mordant treatment brought good result in color difference and dyeing fastness. 1. Compared with silk, wool and nylon, the dyeability of cotton was the worse. The color difference of cotton was 18.81 without mordants , and 24.05 with mordant. The dyeability of cotton was much increased by mordants such as potassiumdichromate, copper sulfate, iron sulfate and salt water. The color of cotton was turned into yellow-green in potassium dichromate and yellow-green with deep green in copper sulfate. The mercerization and the chitosan treatment of cotton made the improvement in color, dyeability, laundering fastness, abrasion fastness. 2. The color difference of silk was 3 times as high as cotton. It showed the similar degree with the chitosan treated-cotton. That is to say, silk had good dyeability because it contains amino group and carboxyl group. The dyebility of silk was increased by a mordant such as iron sulfate, potassium dichromate and salt water, Drycleaning fastness showed 5 grade, abrasion-fastness was high over 4~5 grade and sunlight fastness showed 1 grade in all case. 3. The color difference of wool was the best among four fabrics because of 18 kinds of amino acids. It is considered that the sulfate of wool has the polarity and help metal- mordants get the better dyeability such as potassium dichromate, iron sulfate, copper sulfate, and aluminum chloride. The color was reddish-green in potassium dichromate, yellow.greenin iron sulfate and copper sulfate, and yellow in the rest mordants. Drycleaning fastness of wool showed over 4~5 grade, abrasion fastness 5 grade. Sunlight-fastness 1 grade. 4. The dyeability of nylon was almost same because of the similar molecular structure with silk. The clarity of color was poor. The color was yellow-green in copper sulfate and yellow in the rest mordants. Laundering fastness and abrasion-fastness of nylon was good. Sunlight- fastness represented 1 grade.

• Journal of Conservation Science
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• v.32 no.3
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• pp.321-331
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• 2016

Textiles damage caused by acetaldehyde($CH_3CHO$) is not clear as compared to other materials. Total 20 specimens were prepared using 4 different materials (silk, cotton, ramie, hemp) after dyed with 4 colors (undyed, red, yellow, blue, black). The specimens were exposed to $CH_3CHO$ gas in the test chamber. First, textile specimens' damage by differert concentration of acetaldehyde(0.1, 0.5, 1, 10, 100, 500, 1000 ppm) was tested. Second, accelerared damage to the textile specimens were tested according to the temperature and humidity conditions at the damage levels. Third, damage of deliberately degraded textile specimens were examined at the damage levels. After the exposure, optical, chemical, and physical evaluation was carried out. As a result, at 1000 ppm/day, the color difference of cotton_yellow has increased. At the condition of $25^{\circ}C-80%$, $30^{\circ}C-50%$, $30^{\circ}C-80%$, the color difference of yellow specimens has increased and grey scale rating has decreased. At $30^{\circ}C-80%$, acetate of cotton_undyed increased and the pH of silk_undyed decreased. In the case of deliberately degraded textile specimens, actetate concentration of black specimens increased. In conclusion, damage to the traditional fabric by acetaldehyde is not impact. However, it is expected that yellow specimens will be bleach and black specimens' actetate concentration will be increase.

• Science of Emotion and Sensibility
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• v.14 no.2
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• pp.207-220
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• 2011

The purpose of this study is to investigate sensibility words related with eco-friendly in the two media fashion magazines and internet newspapers and to analysis their appearance frequency and changes by the year through 1999~2010. Most frequently used words are 'nature, eco, cotton, natural fiber, health, fresh, clear, preservation, harmony, com fiber, and Lohas'. The words are divided in 4 groups: 'Nature/Environment, Material/Fiber, Human, and Adjectives/Micell'. A point of appearing time is analyzed: 'ecology, memory-shape material, organic, spa' were used before 2000, 'nature environment, eco-friendly, stretch material, wellbeing, substitute, recycling' were in 2000-2001, 'smart material, eco material, green' in 2002-2003, 'coolbiz, Lohas, natural dye' in 2004-2005, 'herb medicine, sustainable, warmbiz' in 2006-2007, 'greensumer, greenlife, solar energy, forest bath' in 2008-2009. Looking into their changes, in early 2000, the words of eco-friendly emotion and sensibility had appeared frequently relatively, but later on they decreased, and again recently increased showing highest appearing frequency. 'Nature/Environment' words have appeared recently very much, while 'Human' sensibility words have not changed much or decreased a little. 'Adjective/Micell' words has increased little bit recently. 'Material/Fiber' words showed decrease at fashion magazine, while they increased at the pages of internet news.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.142-146
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• 2013

The fluorescent proteins are generally denatured by heat treatment and thus lose their color. The normal reeling method includes processing by drying and cooking the cocoons near $100^{\circ}C$ before reeling. Therefore, the usual processing method cannot be used for making colored fluorescent silks. To develop a method that is applicable to producing transgenic silk without color loss, we develop reeling methods adequate for a recombinant fluorescence cocoons. It was found that the fluorescence cocoons keep their native color when dried at temperatures lower than $60^{\circ}C$ for 15 h. Also, a new cooking method to soften the fluorescent cocoons was developed: the cocoons were soaked in a solution of 0.2% sodium carbonate ($Na_2CO_3$)/0.1% nonionic surfactant (Triton X100) at $60^{\circ}C$ and then placed under vacuum. The repeated vacuum treatments enabled complete penetration of the solution into the cocoons, and the cocoons were thus homogenously softened and ready for reeling. In this state, the cooked cocoons can be reeled by an automated reeling machine. Our results suggest that drying and cooking of the cocoons at low temperature enables the subsequent reeling of the colored fluorescent silks by an automatic reeling machine without color loss and can produce silks that can be used for making higher value-added silk materials.

• Proceedings of the Korean Society of Community Living Science Conference
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• 2009.09a
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• pp.103-103
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• 2009

한국 최초의 벼 재배지이고 농경문화의 근원지인 김포시는 경기도 북서부지역에 위치하고 있다. 북쪽과 동쪽으로 한강을 사이에 두고 있고 강화만 및 한강 하구부 염하 등이 3면을 둘러싸여 있어 김포반도라 한다. 서쪽에 문수산(376m), 장릉산(150m), 수안산(147m) 등의 낮은 산들이 소구릉지를 이루고 있으며 한강에 의한 토사의 운반 퇴적으로 이루어져 농경에 적합한 김포평야를 이룬다. 이런 특징을 갖고 있는 김포지역 향토자원 조사를 위하여 고문헌, 군지, 학술지 등의 자료를 통해 총 563종의 향토자원 목록을 정리하였다. 향토자원의 유형분류를 해보면 인물 193종(34.3%), 자연생태환경 140종(24.9%), 유물유적 136종(24.2%), 민속풍속 79종(14.0%), 전통식품 특산품 15종(2.7%)의 비율로 나타났다. 이 중 활용성이 있는 것으로 판단되는 자원을 지역 전문가 협의회를 통해 1차 선발 평가하여 기본조사를 실시하였고, 향토자원의 가치, 산업화 가능성 평가를 통해 2차 심층조사 대상을 선정하였다. 김포시 향토자원 목록 총 563종 중, 179종이 기본조사 자원으로 선정되어 기본조사를 실시한 결과, 설화 전설자원이 34종(19.0%), 전통제전 자원이 19종(10.6%), 유적사적지와 건축자원이 각 18종(10.1%), 수변 및 해양자원 자원이 15종(8.4%), 조각 자원이 15종(8.4%), 마을자원이 10종(5.6%) 등으로 나타났다. 각 자원의 명칭을 보면 설화 전설은 선돌이 전설, 김삿갓의 일화, 형제투금, 애기봉전설 등이었고, 전통제전은 김포통진두레놀이, 손돌공 진혼제, 대명리 풍어제 등의 자원이, 유적사적지는 덕포진(사적 제292호), 수안산성(지방기념물 제159호), 장릉(사적 제202호) 등으로 조사되었는데, 특히 지석묘의 분포가 많았다. 기본조사 자원을 대상으로 지역전문가 2차 협의회를 통해 향토자원의 가치, 산업화 가능성 등을 평가하여 53종을 선정하였고, 유물유적 24종(45.3%), 민속풍속 12종(22.6%), 자연 생태환경 10종(18.9%), 전통식품 특산품 4종(7.5%), 인물 3종(5.7%) 비율로 나타났다. 향토자원 산업화는 생산물 품질고급화나 농산물 가공분야가 점차 감소하고 천연염색 공예, 관광, 문화상품 등으로 다각화되고 있으므로 김포시의 농촌문화와 전통지식기술자원이 지자체와 지역주민들에 의해 활성화되기를 기대한다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.3
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• pp.349-354
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• 2014

Elevation of intracellular calcium ($Ca^{{+}{+}}$) triggers degranulation of mast cells by bypassing receptor activation. Flos Sophora japonica L. has been used as a natural dying source and has been reported to have biological activities such as anti-inflammatory and anti-allergic effects through $Fc{\varepsilon}RI$ and IgE crosslinking. In the present investigation, we report the regulatory effect of ethanolic extract of Flos Sophora japonica L. (S.F) on allergic mediators produced by $Ca^{{+}{+}}$ ionophore activation in mast cells. S.F significantly inhibited calcium ionophore (A23187)-induced interleukin (IL)-4 and tumor necrosis factor (TNF)-${\alpha}$ production as well as mast cell degranulation. Furthermore, administration of S.F suppressed allergic reactions in a 2,4-dinitrofluorobenzene (DNFB)-induced allergic dermatitis mouse model. Both oral administration and ear painting using 50 mg/kg of S.F significantly reduced levels of cytokines such as IL-4, TNF, and interferon-${\gamma}$ in ear tissues compared to the DNFB alone-treated group. Serum IgE level in the S.F-treated group also decreased compared to the DNFB alone-treated group. Weights of spleens and lymph nodes in the S.F-treated groups also decreased compared to the control group. Considering the data, we conclude that S.F mediates its anti-allergic effects not only through $Fc{\varepsilon}RI$ stimulation but also $Ca^{{+}{+}}$ influx in mast cells.

• Polymer(Korea)
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• v.30 no.1
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• pp.14-21
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• 2006

Tissue engineering techniques require the use of a porous biodegradable/bioresorbable scaffold, which server as a three-dimensional template for initial cell attachment and subsequent tissue formation in both in vitro and in vivo. Small intestinal submucosa (SIS) has been investigated as a source of collagenous tissue with the potential to be used as biomaterials because of its inherent strength and biocompatibility. SIS-loaded poly(L-lactide-co-glicolide)(PLGA) scaffolds were prepared by solvent casting/particle leaching. Characterizations of SIS/PLGA scaffold were carried out by SEM, mercury porosimeter, and so on. Muscle-derived stem cells can be differentiated in culture into osteoblasts, chondrocytes, and even myoblasts by the controlling the culture environment. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide(MTT) test. Osteogenic differential cells were analyzed by alkaline phosphatase(ALP) activity. SIS/PLGA scaffolds were implanted into the back of athymic nude mouse to observe the effect of SIS on the osteoinduction compared with controlled PLGA scaffolds. Thin sections were cut from paraffin embedded tissues and histological sections were conducted hematoxylin and eosin (H&E), Trichrome, and von Kossa. We observed that bone formatioin of SIS/PLGA hybrid scaffold as natural/synthetic scaffold was better thean that of only PLGA scaffold. It canb be explained that SIS contains various kinds of bioactive molecules for osteoinduction.

• Journal of Life Science
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• v.21 no.6
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• pp.783-787
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• 2011

The effects of thaumatin isolated from katemfe fruit of Thaumatococcus daiellii Benth on 3T3-L1 preadipocyte differentiation was investigated in vitro. 3T3-L1 adipocytes were treated with various concentrations of thaumatin ranging in 0-5 ${\mu}M$. Thaumatin reduced fat accumulation in differentiated 3T3-L1 adipocytes in a dose-dependent manner. 3T3-L1 cell proliferation was 97.0 and 88.3% at 1 and 3 ${\mu}M$ after 8 days of thaumatin treatment, respectively. Thaumatin showed a potent inhibitory effect on stained lipid droplets at a concentration of 3 ${\mu}M$. Thaumatin reduced triglyceride accumulation in differentiated 3T3-L1 cells in a dose-dependent manner, compared with positive control cells. This study provides basic information on the anti-obesity activity of thaumatin.