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Biodisc Tissue-Engineered Using PLGA/DBP Hybrid Scaffold  

Ko, Youn-Kyung (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Kim, Soon-Hee (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Jeong, Jae-Soo (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Ha, Hyun-Jung (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Yoon, Sun-Jung (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Rhee, John-M. (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Kim, Moon-Suk (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Lee, Hai-Bang (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Khang, Gil-Son (BK-21 Polymer BIN Fusion Research Team, Chonbuk National University)
Publication Information
Polymer(Korea) / v.31, no.1, 2007 , pp. 14-19 More about this Journal
Abstract
Demineralized bone particle (DBP) has been used as one of the powerful inducers of bone and cartilage tissue specialization. In this study, we fabricated DBP/PLGA scaffold for tissue engineered disc regeneration. We manufactured dual-structured scaffold to compose inner cylinder and outer doughnut similar to nature disc tissue. The DBP/PLGA scaffold was characterized by porosity, wettability, and water uptake ability. We isolated and cultured nucleus pulposus (NP) and annulus fibrosus (AF) cells from rabbit intervertebral disc. We seeded NP cells into the inner core of the hybrid scaffold and AF cells into the outer portion of it. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl) -2,5- diphenyltetrazolium -bromide (MTT) test. PLGA and PLGA/DBP scaffolds were implanted in subcutaneous of athymic nude mouse to observe the formation of disc-like tissue in vivo. And then we observed change of morphology and hematoxylin and eosin (H&E). Formation of disc-like tissue was better DBP/PLGA hybrid scaffold than control. Specially, we confirmed that scaffold impregnated 20 and 40% DBP affected to proliferation of disc cell and formation of disc-like tissue.
Keywords
biodisc; demineralized bone particle; scaffold; annulus fibrosus; nucleus pulposus;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 26  (Related Records In Web of Science)
Times Cited By SCOPUS : 12
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