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http://dx.doi.org/10.3746/jkfn.2014.43.3.349

Anti-allergic Effect of Ethanolic Extract of Flos Sophora japonica L. on Ca++ Ionophore Stimulated Murine RBL-2H3 Cells  

Xiao, Sun (Dept. of Biotechnology, College of biomedical and Health Science, Konkuk University)
Jiang, Jun (Dept. of Biotechnology, College of biomedical and Health Science, Konkuk University)
Shim, Do-Wan (Dept. of Biotechnology, College of biomedical and Health Science, Konkuk University)
Kim, Tae-Kweon (Yakcho Life Co.)
Kang, Tae-Bong (Dept. of Biotechnology, College of biomedical and Health Science, Konkuk University)
Lee, Kwang-Ho (Dept. of Biotechnology, College of biomedical and Health Science, Konkuk University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.43, no.3, 2014 , pp. 349-354 More about this Journal
Abstract
Elevation of intracellular calcium ($Ca^{{+}{+}}$) triggers degranulation of mast cells by bypassing receptor activation. Flos Sophora japonica L. has been used as a natural dying source and has been reported to have biological activities such as anti-inflammatory and anti-allergic effects through $Fc{\varepsilon}RI$ and IgE crosslinking. In the present investigation, we report the regulatory effect of ethanolic extract of Flos Sophora japonica L. (S.F) on allergic mediators produced by $Ca^{{+}{+}}$ ionophore activation in mast cells. S.F significantly inhibited calcium ionophore (A23187)-induced interleukin (IL)-4 and tumor necrosis factor (TNF)-${\alpha}$ production as well as mast cell degranulation. Furthermore, administration of S.F suppressed allergic reactions in a 2,4-dinitrofluorobenzene (DNFB)-induced allergic dermatitis mouse model. Both oral administration and ear painting using 50 mg/kg of S.F significantly reduced levels of cytokines such as IL-4, TNF, and interferon-${\gamma}$ in ear tissues compared to the DNFB alone-treated group. Serum IgE level in the S.F-treated group also decreased compared to the DNFB alone-treated group. Weights of spleens and lymph nodes in the S.F-treated groups also decreased compared to the control group. Considering the data, we conclude that S.F mediates its anti-allergic effects not only through $Fc{\varepsilon}RI$ stimulation but also $Ca^{{+}{+}}$ influx in mast cells.
Keywords
Flos Sophora japonica; $Ca^{{+}{+}}$ ionophore (A23187); anti-allergy; degranulation;
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