• Title/Summary/Keyword: 천연 다당류

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Free Amino Acid and Polysaccharide Content of Submerged Mycelial Culture of Fomitopsis pinicola (귤피 및 녹차추출물에서 배양한 소나무잔나비버섯(Fomitopsis pinicola) 균사체의 유리아미노산 및 다당류 함량)

  • Jang Kyung-Ho;Shin Kyung-Ok;Kim Soon-Dong
    • Food Science and Preservation
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    • v.12 no.4
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    • pp.379-386
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    • 2005
  • Free amino acids and polysaccharide content of submerged mycelial culture of Fomitopsis pinicola using $2\%$ citrus peel water extracts(CP), $2\%$ green tea water extracts(GT) and yeast-malt broth(YM) were investigated. Cultivations were conducted at $30^{\circ}C$ and 150 rpm for 10 days. Yields of the mycelium as fresh weight basis in GT-, CP- and YM-broth were $42.3\%,\;34.2\%\;and\;9.89\%$, and their turbidity(OD at 660 nm) of the broth without mycelium were 0.14, 0.16 and 0.22, respectively. Total free amino acid content in the mycelium were 928.19 $mg\%$ in YM, 1060.53 $mg\%$ in CP, 764.83 $mg\%$ in GT, and the major free amino acid was lysine in YM, glutamic acid in CP and GT. Total free exo-amino acid contents were 659.75 $mg\%$ in YM, 954.55 $mg\%$ in CP, 838.69 $mg\%$ in GT, and the major in the all broths was glutamic acid Total amino acid derivatives content of the mycelium were in order of CP>GT>YM, and the major was cystathionine in YM, hydroxy proline in CP and GT. The major among exo-amino derivatives was hydroxy proline in the all broth. Exo-AIS content was in order of CP>GT>YM. Acid soluble polysaccharide content of the mycelium was GT($0.69\%$)>YM($0.39\%$)>CP($0.18\%$). The exo-polysaccharide content was in order of GT($0.87\%$)>CP($0.69\%$)>YM($0.09\%$). Alkali soluble polysaccharide content of the mycelium was in order of CP($5.21\%$)>GT($5.18\%$)>YM($4.56\%$), and exo-polysaccharide was in order of GT($6.79\%$)>YM($3.57\%$)>CP($3.01\%$). The alkali soluble polysaccharide eluted from mycelium cultivated in CP broth was supposed to polysaccharide(about 500,000 daltons) composed of hexose and uronic acid bounded with protein(below 10,000 daltons).

The Crosslinking Characteristic of Poly(vinyl alcohol) by Natural Dye (천연 염료에 의한 폴리(비닐 알코올)의 가교 특성)

  • Kim, Gwan-Hoon;Kim, Hyo-Gap;Kang, Ho-Jong
    • Polymer(Korea)
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    • v.35 no.1
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    • pp.72-76
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    • 2011
  • The physical properties of crosslinked poly(vinyl alcohol)(PVA) by natural dyes as crosslinking agents were investigated and a comparison was made with chemically crosslinked PVA by Polycup 172. It was found that natural anthocyanin and crocin made possible to crosslink PVA physically through the hydrogen bonding of OH in both PVA and natural dyes in the present with NaCl as a catalyst. The water swellability dramatically decreased and the physical crosslinking led to decreasing of crystallinity of PVA. The lowering of thermal stability was noticed in the physically crosslinked PVA compared to chemically crosslinked PVA due to its lower crosslink density. However even natural dyes have polysaccharides in their chain, their thermal stability was higher than uncrosslinked PVA.

Antioxidant activity of partially characterized polysaccharides from the edible mushroom Pleurotus djamor var. roseus

  • Raman, Jegadeesh;Sivakumar, Archana;Lakshmanan, Hariprasath;Raaman, Nanjian;Shin, Hyun-Jae
    • Journal of Mushroom
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    • v.19 no.3
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    • pp.140-149
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    • 2021
  • Mushroom-derived polysaccharides, which are the primary bioactive constituents, are beneficial for human health. Polysaccharides have immuno-modulation, antitumor, and antioxidant properties. Additionally, they have antiviral properties and protect against chronic radiation stress. In this study, high yield water-soluble polysaccharides were obtained from Pleurotus djamor var. roseus basidiocarps. The crude polysaccharide (CP) was extracted sequentially by hot water and ethanol precipitation. The yield of the brown CPs was 5.6% dw. Diethylaminoethyl cellulose and Sepharose-6B column chromatography of CPs generated several fractions. Total glucan content was determined in all the fractions. The F1 fraction displayed the highest sugar content and was considered as a purified polysaccharide (PP). The total glucan and β-glucan content in the four fractions ranged between 76.85-2.95% and 75.08-1.46%, respectively. The yield of the PPs was 300 mg, and it was obtained as a white powder. The PPs were characterized by Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography. The FTIR spectral details confirmed the presence of a xylopentose polysaccharide. The antioxidant activity of the PPs was evaluated using in vitro 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and superoxide radical scavenging assay. The PPs showed strong DPPH free radical and superoxide anion radical scavenging activities in a dose-dependent manner. Purified PPs free of phenolics, protein, and carbohydrates were mainly responsible for the radical scavenging activity. The data suggest the potential of PPs as natural antioxidants.

Anti-inflammatory effect of zaluzanin C on lipopolysaccharide-stimulated murine macrophages (지방질다당류로 자극한 마우스 대식세포에 있어서 zaluzanin C의 항염증 효과)

  • Kang, Ye Rim;Lee, Hee Won;Kim, Yoon Hee
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.392-397
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    • 2016
  • Zaluzanin C is a sesquiterpene lactone isolated from Ainsliaea acerifolia, a Korean medicinal plant. In the present study, the anti-inflammatory effects of zaluzanin C were demonstrated in lipopolysaccharide (LPS)-stimulated murine macrophages (RAW264.7 cells). Zaluzanin C inhibited the release of nitric oxide (NO) by alleviating the protein expression of inducible NO synthase in LPS-treated RAW264.7 cells. Furthermore, it suppressed the release of interleukin-6 induced by LPS. Zaluzanin C was also found to block the translocation of the p65 subunit of nuclear factor-kB from the cytosol to the nucleus, which is one of the underlying mechanisms of the anti-inflammatory action of zaluzanin C. These data suggest the potential of zaluzanin C in the treatment of inflammatory diseases.

Effect of Erythritol on Glucosyl Transferase and Fructosyl Transferase Gene Expression in Streptococcus mutans (Streptococcus mutans의 Glucosyl Transferase와 Fructosyl Transferase 유전자 발현에 대한 Erythritol의 효과)

  • Young-Nam PARK;Jae-Ki RYU
    • Korean Journal of Clinical Laboratory Science
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    • v.55 no.3
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    • pp.151-158
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    • 2023
  • Erythritol is a sweetener produced by yeast from glucose and a natural sugar found in fermented foods such as mushrooms, wine, fruits, rice wine, and soy sauce. Correct information and basic data when producing or using products for preventing dental caries by checking the gene expression patterns of glucosyl transferase (GTF) and fructosyl transferase (FTF) of Streptococcus mutans in erythritol and other sweeteners it was implemented to provide. Erythritol inhibited the growth of Streptococcus mutans, which is involved in dental caries. When used as a sweetener to replace sucrose, erythritol had an excellent caries-preventative effect. In particular, erythritol reduced the expressions of gtfB, gtfC, gtfD, and FTF, which are related to the synthesis of extracellular polysaccharides, and thereby reduced the formation of dental plaque and the attachment rate of bacteria to tooth surfaces. The study shows erythritol has potential use as an anticariogenic sweetener that inhibits the mechanism underlying caries caused by Streptococci.

당류산업

  • 임재각
    • Food Industry
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    • s.181
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    • pp.38-67
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    • 2004
  • 예로부터 인간은 단맛에 강한 욕구를 가지고 있었다. 따라서 설탕, 포도당, 과당 등 단맛이 강한 감미료를 선호하여 왔고, 현재까지도 식품 제조시 가장 널리 사용되고 있다. 그러나 최근에는 국민의 건강에 대한 관심이 고조 되고 설탕으로 대표되는 전통적인 일반 감미료의 충치, 비만, 당뇨병 유발 등의 부정적인 측면이 부각되고 있을 뿐만 아니라, 식품에 대한 건강 기능성이 강조되면서 기능성 감미료가 주목 받고 있다. 특히 고도의 경제성장 및 국민 소득의 향상에 따라 차별화된 기능성 감미료의 생산을 요구하는 실정이다. 이와 관련하여, 현재 식품 산업계에서는 올리고당류, 당알콜류, 고감미 감미료류 등에 많은 관심을 가지고 연구 개발에 집중하고 있다. 올리고당류의 대표적인 제품은 프락토올리고당, 갈락토올리고당, 이소말토올리고당 등이다. 현재 올리고단 시장은 성숙기로서 성장속도가 둔화되고 있으나, 최근 American Association of Cereal Chemists(AACC)등 세계적인 협회에서 수용성 식이섬유를 다당류, 올리고당, 리그닌 등을 포함하여 새롭게 정의하였고 이에 따라 해외에서는 프락토올리고당을 중심으로 수용성 식이섬유로 용도 사용이 가능하게 되었을 뿐만 아니라, 식이섬유로 표기도 가능하게 되어 수요가 증가할 것으로 예상된다. 국내에서는 아직 올리고당을 식이섬유로 사용하지는 못하고 있으나, 식품 공전 개정을 통해 식이섬유로 표기 가능하도록 수정될 전망이다. 당알콜류는 솔비톨 및 자일리톨이 주류를 이루고 있다. 솔비톨은 시장에 가장 먼저 소개된 당알콜류인데 저가여서 그동안 가장 큰 시장을 형성하고 있었으나 최근 3년 동안 자일리톨껌이 최대의 히트 상품이 되면서 자일리톨이 급성장하였다. 그 외에는 만니톨, 에리스리톨, 말티톨, 이소말트 등이 있다. 최근에는 말티톨을 중심으로 다양한 응용 연구 및 이소말트 및 에리스리톨 등을 생산 판매하고 있는 다국적 기업의 판촉 활동 등으로 당알콜류 시장의 새로운 변화가 예상된다. 고감미 감미료류는 스테비오사이드와 아스파탐이 주류를 이루고 있었지만, 최근 아세설팜 K 및 수크랄로스가 잇따라 식품으로써 사용 허가를 받으면서 시장이 재편되고 있다. 하지만, 해외에 비하여 국내의 고감미 감미료 시장은 매우 작고 성장률도 낮은 편인데 그 이유로는 국민 정서상 천연이 아닌 합성에 대한 부정적 인식과 맛에 대한 거부감 때문인 것으로 생각된다. 이와 관련하여 최근에는 다양한 감미질 및 Multi sweetener system 연구를 통해 설탕과 유사한 맛을 내는 혼합 기술 개발에 관한 연구가 진행되고 있다. 따라서 향후에는 해외의 선진국과 같이 고감미 감미료 시장도 점차적으로 확대될 것으로 예상된다. 기타 대체 감미료로는 타가토스, 트레할로스, 아라비노스 등이 있다. 이중에서 특히 해외에서 크게 기대를 모으고 있는 타가토스는 최근 국내 인증을 받아 시장 진출을 준비 중에 있다. 아라비노스는 양질의 감미를 갖는 천연 펜토오스인데 연구용 시약 이외에는 지금까지 거의 이용되지 않았다. 그러나 최근 아라비노스가 설탕의 소화흡수를 선택적으로 억제한다는 흥미 있는 결과가 임상 및 동물실험에서 확인되어 향후 제품으로써 개발을 기대할 수 있게 되었다. 이들 감미료는 기존 설탕이 가지는 문제점을 많은 부분 극복할 것으로 여겨지며, 이로 인해 수요는 점차 증가할 것으로 생각되며, 높은 경제적 부가 가치를 창출할 것으로 예상되어 진다. 생산 기술 적인 측면에서 건강 지향적 천연소재 감미료에 대한 선호가 날로 증가하는 추세에 따라 기존에 주로 사용되었던 화학합성법보다는 천연 이미지의 생물공학적인 방법에 의한 감미료 생산이 각광을 받을 것으로 예상된다. 생물공학적인 방법을 감미료 소재 개발에 응용하게 되면, 기존에 사용되었던 화학합성법 등이 가지는 환경적인 문제, 반응 부산물 생성에 따른 분리, 정제에서의 비용증가 등의 문제점을 해결할 수 있을 것으로 여겨지며, 고정화 방법의 도입 등을 통해 생산성 향상을 도모할 수 있다. 본 장에서는 감미료 소재 개발에 건강 지향적 국민 정서를 반영하고, 국내외 감미료 시장의 흐름을 파악하여 당류 산업의 발전적인 변화에 대처하고자 하였으며, 이를 통해 당류산업의 고부가가치 첨단산업으로서의 육성방안을 논의하고자 하였다.

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Production of the Convertible Enzyme of Ginsenoside $Rb_1$ by Rhizopus japonicus (Rhizopus japonicus 에 의한 Ginsenoside $Rb_1$ 전환효소의 생산조건)

  • Kim, Sang-Dal
    • The Korean Journal of Mycology
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    • v.17 no.1
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    • pp.31-34
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    • 1989
  • In ginseng saponin, $ginsenoside-Rb_1$ was contained the most abundantly. But ginsenoside-Rd which is similar to ginsenoside $Rb_1$ in structure was known to be superior to $ginsenoside-Rb_1$ pharmaceutically. A strain of Rhizopus japonicus is able to produce the convertible enzyme which can convert selectively $ginsenoside-Rb_1$ to ginsenoside-Rd without the change of any other ginsenoside. The strain can produce the most enzyme after 5 day-culture on wheat bran medium. The enzyme production was promoted best efficiently by addition of red ginseng powder in ginseng products, xylose in sugars, laminarin in polysaccharides, naringin in flavonoids, and potassium nitrate in nitrogen substrates.

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Screening of Hyaluronidase Inhibitor in Korean Medicinal Plants (천연물로부터 히알루로니다아제 저해제 검색)

  • Hwang, Seon Gu;Yang, Anna;Kim, Soo Jung;Kim, Min Kee;Kim, Sung Soo;Oh, Hyun Jung;Lee, Jung Dae;Lee, Eun Ju;Nam, Kung-Woo;Han, Man-Deuk
    • Journal of Life Science
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    • v.24 no.5
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    • pp.498-504
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    • 2014
  • Mammalian hyaluronidases (HAase, EC 3.2.1.35) are a family of enzymes that hydrolyse N-acetyl-D-glucosamine (1-4) glycosidic bonds in hyaluronic acid, which is found in skin, cartilage, and the vitreous body. Although HAase is generally present in an inactive form within subcellular lysosomes, it is released in an active form in some types of inflammation and tissue injuries, thereby contributing to the inflammatory response. The HAase inhibitory activity of 500 methanolic extracts of 500 species from medicinal plants was screened using a Morgan microplate assay. The viscosity of the hyaluronic acid was measured with an Ubbelohde viscometer. Three MeOH extracts inhibited more than 50% of HAase activity at a concentration of 2 mg/ml. HAase inhibitory rates (%) of three species of medicinal plant extracts, Styrax japonica, Deutzia coreana, and Osmanthus insularis were 57.28%, 53.50%, and 53.19%, respectively. The rate of HAase inhibition of the extracts was dose dependent. In the HAase inhibitory assay using the Ubbelohde viscometer, the results were in good agreement with the results from the Morgan assay. The results suggest that HAase inhibitory compounds extracted from the stem of S. japonica, D. coreana, and O. insularis might be multifunctional and prevent the degradation of hyaluronic acid and the induction of allergic reactions and inflammation.

Bacterial Community of Natural Dye Wastewater Treatment Facility (천연염색 폐수처리시설의 세균 군집)

  • Hwang, Yeoung Min;Kim, Dae Kuk;Lee, Ji Hee;Baik, Keun Sik;Park, Chul;Seong, Chi Nam
    • Journal of Life Science
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    • v.24 no.4
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    • pp.393-402
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    • 2014
  • Culture-dependent and culture-independent denaturing gradient gel electrophoresis (DGGE) analyses were employed to investigate the bacterial community associated with a natural dye wastewater treatment facility. A total of 104 (influent water, 48 strains; aeration tank, 25; settling tank, 31) bacterial strains were isolated. Based on the 16S rRNA gene sequences comparison analysis, the isolates belonged to four phyla: Proteobacteria, Actinobacteria, Firmicutes, and Bacteriodetes. Seventeen DGGE bands representing dominant taxa in each sample were cloned and partially sequenced. The same four phyla were detected by DGGE fingerprinting. The most dominant taxon retrieved by both methods was the member of the phylum Proteobacteria with Alphaproteobacteria as the predominant class. The bacterial community associated with the natural dye wastewater treatment facility is composed of parasites of animals and plants, decomposers of polysaccharides and dyes, and producers of extracellular polysaccharides.

Manufacture and Characteristics of Peel-off Pack for Natural Cosmetics Using Pullulan and Polysaccharides (Pullulan과 Polysaccharides를 이용한 천연화장품용 필 오프 팩의 제조 및 특성)

  • Jun Soo Kwak;So Young Jung;So Min Lee;Seok-Ju Lee;Sofia Brito;Byungsun Cha;Hyojin Heo;Lei Lei;Sang Hun Lee;Ha-Hyeon Jo;You-Yeon Chun;Ye Ji Kim;Hyung Mook Kim;Mi-Gi Lee;Byeong-Mun Kwak;Bum-Ho Bin
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.49 no.1
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    • pp.67-74
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    • 2023
  • In this study, for a natural cosmetics market, we sought to explore alternatives that can replace polyvinyl alcohol (PVA) of peel-off packs. A peel-off type pack was prepared by combining pullulan, a water-soluble polysaccharide, and other polysaccharides (sodium hyaluronate, cellulose gum, hydroxyethyl cellulose, sodium alginate, corn starch), and the pH, viscosity, and stability against temperature of each peel-off type pack were confirmed. The thickness and tensile strength of the manufactured film were measured for comparison with the PVA peel-off type pack, and applicability, drying speed, and removal degree were measured. Among them, the pullulan-sodium hyaluronate peel-off type pack showed excellent film formation ability to replace the peel-off type pack containing PVA with 5.12% thin film thickness and 4.23% high film tensile strength. When applied to actual skin, the degree of spread of the pack, the usability that can be uniformly applied, and the formation and removal strength of the film when removed after drying were also similar to the peel-off type pack containing PVA. Therefore, it was confirmed that the film formed of pullulan-sodium hyaluronate showed enough physical properties to replace the PVA of the peel-off type pack as a natural peel-off type pack.