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http://dx.doi.org/10.5352/JLS.2014.24.5.498

Screening of Hyaluronidase Inhibitor in Korean Medicinal Plants  

Hwang, Seon Gu (Department of Biology, College of Natural Science, Soonchunhyang University)
Yang, Anna (Department of Biology, College of Natural Science, Soonchunhyang University)
Kim, Soo Jung (Department of Biology, College of Natural Science, Soonchunhyang University)
Kim, Min Kee (Department of Biology, College of Natural Science, Soonchunhyang University)
Kim, Sung Soo (Department of Biology, College of Natural Science, Soonchunhyang University)
Oh, Hyun Jung (Department of Biology, College of Natural Science, Soonchunhyang University)
Lee, Jung Dae (Department of Biology, College of Natural Science, Soonchunhyang University)
Lee, Eun Ju (Department of Biology, College of Natural Science, Soonchunhyang University)
Nam, Kung-Woo (Department of Biology, College of Natural Science, Soonchunhyang University)
Han, Man-Deuk (Department of Biology, College of Natural Science, Soonchunhyang University)
Publication Information
Journal of Life Science / v.24, no.5, 2014 , pp. 498-504 More about this Journal
Abstract
Mammalian hyaluronidases (HAase, EC 3.2.1.35) are a family of enzymes that hydrolyse N-acetyl-D-glucosamine (1-4) glycosidic bonds in hyaluronic acid, which is found in skin, cartilage, and the vitreous body. Although HAase is generally present in an inactive form within subcellular lysosomes, it is released in an active form in some types of inflammation and tissue injuries, thereby contributing to the inflammatory response. The HAase inhibitory activity of 500 methanolic extracts of 500 species from medicinal plants was screened using a Morgan microplate assay. The viscosity of the hyaluronic acid was measured with an Ubbelohde viscometer. Three MeOH extracts inhibited more than 50% of HAase activity at a concentration of 2 mg/ml. HAase inhibitory rates (%) of three species of medicinal plant extracts, Styrax japonica, Deutzia coreana, and Osmanthus insularis were 57.28%, 53.50%, and 53.19%, respectively. The rate of HAase inhibition of the extracts was dose dependent. In the HAase inhibitory assay using the Ubbelohde viscometer, the results were in good agreement with the results from the Morgan assay. The results suggest that HAase inhibitory compounds extracted from the stem of S. japonica, D. coreana, and O. insularis might be multifunctional and prevent the degradation of hyaluronic acid and the induction of allergic reactions and inflammation.
Keywords
Hyaluronic acid; hyaluronidase; hyaluronidase inhibitor; medicinal plants; Styrax japonica;
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