• Korean Journal of Plant Resources
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• 제32권1호
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• pp.29-37
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• 2019

This study was conducted to provide a basic data such as leaf morphological characteristics, total 110 individual trees selected from 11 wild population, for selective breeding. As a result of investigation of the twenty morphological characteristics of the leaf, there were statistically significant differences in all leaf characteristics among the populations. Especially, Mt. Mani population had larger leaf length (LL), width (LW) and area (LAR) than other populations. On the other hand, Mt. Beakwoon and Mt. Duryun had smaller leaf size (LL, LW and LAR) among the populations. Its principal component analysis (PCA) results showed that it represented 72.9% accumulated explanation from three principal component. The characteristics such as leaf area, leaf length and leaf width were highly contributed for classification among populations. According to the cluster analysis, the natural S. alnifolia populations were classified into five groups and Mt. Mani population was different from the other population.

• Korean Journal of Plant Resources
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• 제32권2호
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• pp.153-159
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• 2019

In this study, we evaluated the effect of branch (STB) and leave (STL) extracts from Sageretia thea on ${\beta}-catenin$ level in human colorecal cancer cells, SW480 and lung cancer cells, A549. STB and STL dose-dependently suppressed the growth of SW480 and A549 cells. STB and STL decreased ${\beta}-catenin$ level in both protein and mRNA level. MG132 decreased the downregulation of ${\beta}-catenin$ protein level induced by STB and STL. However, the inhibition of $GSK3{\beta}$ by LiCl or ROS scavenging by NAC did not block the reduction of ${\beta}-catenin$ protein by STB and STL. Our results suggested that STB and STL may downregulate ${\beta}-catenin$ protein level independent on $GSK3{\beta}$ and ROS. Based on these findings, STB and STL may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer and lung cancer.

• Korean Journal of Plant Resources
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• 제34권4호
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• pp.368-376
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• 2021

This study was to investigate the Taraxinic acid from Taraxacum coreanum on tyrosinase activity and melanogenesis. In B16BL6 cell, Taraxinic acid did not show cytotoxicity even at concentrations of up to 100 ㎍/mL. In addition, tyrosinase inhibitory activity and melanogenesis inhibitory activity were confirmed by stimulation with α-melanocyte stimulating hormone (α-MSH) in the presence of taraxinic acid. Taraxinic acid was added to cells at concentrations of 10, 50 and 100 ㎍/mL and treated for 48 hours to confirm tyrosinase inhibitory activity and melanin production. The tyrosinase inhibitory activity increased in proportion to the amount of the sample, and showed an inhibitory activity of about 54.5% at a concentration of 50 ㎍/mL. Melanin production decreased in proportion to the sample amount, and it was about 62.2% at the concentration of 10 ㎍/mL. From the above results, it was found that Taraxinic acid had higher tyrosinase activity and melanin synthesis inhibitory activity in melanocyte than arbutin. The results suggest that Taraxinic acid can be utilized in natural whitening cosmetics.

• Korean Journal of Plant Resources
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• 제35권2호
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• pp.362-371
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• 2022

This study was conducted to analyze the fragrance characteristics of Cymbidium 'Saelbit' and 'Midan' according to floral organs. As test materials, full bloom flowers were divided into four organs: sepal, petal, labellum, and column. Using the gas chromatography (GC) based electronic nose, fragrance patterns, intensity, and volatile components were analyzed. Principle component analysis (PCA) and discriminant factorial analysis (DFA) plots by electronic nose data showed that volatiles of both cultivars have a distinct difference in fragrance patterns according to the floral organs, and the value of fragrance distance and pattern discrimination index (PDI) between samples was significantly high between control and sepals in both cultivars. Among the main fragrance components, several components including nootkatone were detected in both cultivars and all floral organs. However, few components such as decane were found in specific cultivar or floral organs. These results will provide useful information to select suitable materials with desired fragrance and to enhance the utilization of domestic Cymbidium cultivars. In addition, considering the recent negative perception of artificial ingredients and the growing demand for natural materials, continuous researches on scent properties of promising cultivars are required.

• Journal of Life Science
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• 제31권12호
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• pp.1128-1141
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• 2021

'Angelica' is one of the most traditionally consumed medicinal herbs around Northeast Asia including Korea for treatments of various diseases or health care purposes like hematopoiesis, blood circulation for women, sedative, analgesic, and a tonic medicine etc. Angelica gigas Nakai, a Korean native species of Angelica, is clearly different from the others in containing a high concentration of active ingredients like pyranocoumarines including decursin, decursinol, and decursinol angelate. These compounds have various kinds of positive effects such as anti-tumor activity including the precaution of neutropenia occurred during anticancer drug administration, improvements of metabolic disorders, menstrual irregularity, impairment of renal function, respiration improvement, cognition-enhancement, anti-inflammatory effect, anti-oxidative effect, enhancing fertility and so forth. Thus it implies incredible potentialities in future development for foods and drugs. However, certain purity-related qualities and/or overdose in food products can cause side effects like toxicities; therefore, their safety profiles should also be considered. This review focuses on the positive and negative effects of three pyranocoumarines in Angelica gigas Nakai and some possibilities and considerations for future food and drug products development.

• Journal of Korea Entertainment Industry Association
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• 제13권2호
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• pp.253-259
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• 2019

Prostate cancer(PrCa) is a leading cause of cancer-related death in man. Medicinal plants are exploited for many drugs to treat various ailments. The drugs derived from the plants promote health, augmented the resistance of the body against disease. Pueraia lobata(wild) Ohwi(P. Lobata), kudzu, which is a twining perennial woody herb native to China, Korea, Japan, India, and the United States. Plants such as Moringa oleifera, have hypoglycemic properties and other beneficial properties. The objective of the study was to analyze the effects of kadzu and moringa, natural plant products on antioxidant activity and proliferation of the hormone-sensitive prostate cancer LNCaP cells. MTT assay, flow cytometry analysis were employed to investigate the anticancer mechanism and DPPH assay was determined to the antioxidant activity to scavenge free radicals in extract of these. All two extracts showed significantly antioxidant activity at 10 and 50mg/ml of concentration. kadzu and moringa reduced LNCaP cell viability in a dose dependent manner. Specially moringa extract was more potent cytotoxic than kadzu extract. Statistical analyses revealed kadzu and moringa exhibited significantly higher (P < 0.05) cytotoxicity and antioxidant activity in LNCaP. The finding of this study provides a scientific basis for using kadzu and moringa in future development of chemotherapeutic drugs against hormone-sensitive prostate cancer.

• Journal of Life Science
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• 제33권5호
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• pp.414-421
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• 2023

Cnidium japonicum (C. japonicum) is a type of halophyte that inhabits soil of a high salinity, and according to previous studies, it is known to have antitumor effects. However, the skin's protective effect, particularly against UVB irradiation, has not been revealed. In this study, C. japonicum crude extract was studied to determine its effect on damage to human keratinocytes (HaCaT) induced by UVB irradiation, and ROS assays were performed, the results of which showed that C. japonicum crude extract affects UVB-induced photoaging damage in human keratinocytes. To examine inhibitory effects against the expressions of MMPs, RT-PCR and Western blot assay were performed by treating the crude extract at concentrations of 10, 50, and 100 ㎍/ml by irradiating UVB at 15 mJ/cm². As a result, it was confirmed that the mRNA and protein expression levels of MMP-1, MMP-3, and MMP-9 decreased in the group treated with C. japonicum crude extract, which also effectively regulated the antioxidant defense mechanism pathway by activating JNK, ERK, and p38. In conclusion, the current study suggested the possibility that C. japonicum could be used as a raw material for anti-photoaging cosmeceuticals in the future.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.28-29
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• 2021

애기똥풀(Chelidonium majus L. subsp. asiaticum H.Hara)의 지상부는 백굴채라고 불리며 항암, 항균 등의 효능을 가지고 있어 예로부터 약용으로 이용되었다. 그러나 천연 약용소재로 개발가치가 높음에도 불구하고 실생묘의 대량 확보를 위한 종자번식 관련 연구가 전무한 실정이다. 본 연구에서는 애기똥풀의 발아 및 휴면 특성을 분석함으로써 추후 종자번식을 위한 기초자료를 제공하기 위해 수행되었다. 실험재료로 사용된 애기똥풀 종자는 2019년 6월 경상북도 봉화군에서 채종하였으며 정선 후 건조상태로 실온(25℃)에 보관하였다. 종자의 1000립중은 0.57±0.01g이며, 배종비는 0.21±0.12로 조사되어 형태적으로 미성숙한 배를 가지고 있다. 종자의 활력은 테트라졸륨 검정을 통해 79%로 확인되었다. 종자의 기본 함수율은 7.64±0.25%였으며, 침지 2일 차에 최대함수율(32.6±0.34%)에 도달하였다. 초기 발아검정은 25/15℃ (light/dark, 16/8h)챔버에서 명조건과 암조건으로 구분하여 30일간 진행하였으며, 암조건은 알루미늄 호일로 광을 완전히 차단하고 최종 발아율만 조사하였다. 발아율을 조사한 결과, 명조건은 0.75±0.80%, 암조건은 5.50±1.55%로 조사되어 형태적인 휴면과 생리적인 휴면을 모두 갖는 것으로 판단하였다. 애기똥풀의 생태적인 환경을 고려하여 12주의 Summer temperature(S12, 25/15℃, 16/8h)로 층적하고 연속하여 Autumn temperature(A, 15/10℃, 16/8h)를 4주(A4)와 8주(A8)로 달리하여 종자의 휴면타파를 유도하였다. 층적 후 발아율은 모두 증가되었으며, S12-A4(54%)는 S12-A8(24.5%)에 비해서 휴면타파에 효과적이었다. 휴면타파 효과의 증진을 위해 층적처리한 종자를 500ppm의 GA₃와 GA₄₊₇에 각 24시간동안 4℃에서 침지시킨 뒤 동일한 조건에 파종하였다. GA₃가 처리된 S12-A4는 발아율이 82.0%, S12-A8는 30.8%로 유의적으로 증가하였으나 GA₄₊₇ 처리는 S12-A4(48.5%)와 S12-A8(21.3%)에서 발아율 향상 효과가 없었다. 결론적으로 애기똥풀의 휴면타파를 위해서 12주의 Summer temperature와 4주의 Autumn temperature 변온층적과 추가 GA₃ 처리가 휴면타파를 위한 효과적인 처리방법으로 판단된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.84-84
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• 2020

염증 (Inflammation)은 병원체, 손상된 세포, 자극물질 등으로 인한 손상에 대해 작용하여 조직이나 장기의 손상을 재생하는 작용으로써 신체 방어 기전들 가운데 매우 중요한 역할을 하는 것으로 알려져 있다. 이러한 염증반응이 과다할 경우 각종 염증성 질환 혹은 암 등을 유발하는 원인으로 발전할 수 있어 항염증제의 개발은 전 세계적으로 중요시되고 있다. 선천적 면역을 담당하는 대식세포는 lipopolysaccharide(LPS), 활성산소 (ROS) 등에 의해 자극되어 염증인자 생성에 관여한다. 따라서 본 연구에서는 항염증 소재 개발을 목표로 하며, 국내 천연소재를 활용한 기능성 항염증 소재로 전북 순창군에서 재배된 초석잠(Stachys Sieboldii MIQ.)과 돼지감자 (Helianthus tuberosus L.)를 이용하여 항산화 활성을 평가하고자 하였으며, 대식세포주를 활용한 세포독성 및 항염증 활성에 대한 효능을 확인하고자 하였다. 본 연구는, 전북 순창 지역에서 재배 된 초석잠과 돼지감자를 사용하여 각 조건의 추출 용매, 온도, 시간별 추출물의 Total polyphenol 함량 평가를 통한 최적 추출조건 선정을 진행하고, 선정된 추출조건의 추출물의 항산화 활성을 측정하기 위해 DPPH & ABTS radical scavenging activity 분석 및 Total flavonoids 함량 분석을 통해 항산화 효능 평가를 진행하였다. 또한 항염증 소재로의 활용을 위해 대식세포인 Raw 264.7을 사용하여 농도별 MTT assay를 진행하여 세포독성 평가를 진행하였고, Nitric oxide (NO) 생성억제 효능을 확인하여 항염증 활성을 평가하였다. 실험 결과, Total polyphenol 함량 분석을 통해 최적 추출조건이 선정된 초석잠 (25℃, 주정 60%, 3 h), 돼지감자 (25℃, 주정 40%, 1 h)은 최적 조건에서 약 58 mg GAE/g 및 158 mg GAE/g의 총 폴리페놀 함량을 보였으며, DPPH & ABTS radical scavenging activity 및 Total flavonoids 함량 분석한 결과, 초석잠이 돼지감자보다 더 높은 항산화 활성을 나타내었다. 대식세포 실험에서의 추출물 처리군의 세포독성 측정 결과, 100 ug/mL 이내의 농도에서 독성활성이 나타나지 않음을 확인하였고, Nitric oxide (NO) 생성 억제활성 측정을 통해 LPS 처리군 대비 접종량 100 ug/mL 기준 각각 초석잠 약 47%, 돼지감자 약 49% 수준의 항염증 활성을 확인하였다.

• Korean Journal of Plant Resources
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• 제37권2호
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• pp.101-109
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• 2024

In this study, we investigated the antioxidant effects and active ingredients of Gynura Procumbens extracts obtained through various extraction methods for the development of natural-based cosmetics and pharmaceutical materials. The contents of compounds, total flavonoids, chlorogenic acid, and caffeic acid were compared at different concentrations, revealing the highest content of active ingredients in the 100% ethanol extract. Antioxidant activity assessed by DPPH and ABTS radical scavenging assays showed a concentration-dependent increase in antioxidant activity with the ethanol concentration. Additionally, we validated the DNA damage inhibition and anti-inflammatory effects of Gynura Procumbens extracts through UVB irradiation on Hs68 cell models. The 100% ethanol extract demonstrated significant inhibition of the expression of p-p53, γ-H2AX, iNOS, and COX-2 induced by UVB, indicating its potential in alleviating photodamage effects. Consequently, the efficient extraction of Gynura Procumbens for skin functional material development was confirmed, suggesting the suitability of ethanol or alcohol-based solvents.