• Title/Summary/Keyword: 지혈

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The Improvement Effects of β-Glucan on Adiposity and Serum Lipids Levels in High Fat Diet-Induced Obese Rats (베타-글루칸의 고지방 식이 유도 비만쥐에서 체지방 및 혈청지질 개선효과)

  • Hong, Kyung Hee;Kim, Hyun-Soon;Jang, Ki-Hyo;Kang, Soon Ah
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.6
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    • pp.3973-3981
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    • 2015
  • This study was aimed to investigate the effect of dietary ${\beta}$-glucan obtained from bacterial fermentation on the adiposity and serum lipids level in rats. Sprague-Dawley rats fed high fat diet for 6 weeks to induce obesity, and subsequently fed with 0% (high fat control group), 0.1% or 0.5% ${\beta}$-glucan supplemented high-fat diets (w/w) for another 5 weeks. For comparison, normal control groups fed AIN-76A diet. Supplemented with 0.1% ${\beta}$-glucan resulted in a significant reduction of high-fat induced peritoneal fat and visceral fat development by 16%, 19%, and 28%, respectively(P<0.05). Serum free fatty acid levels were reduced(by 19%), whereas the HDL cholesterol level was increased(by 50%) by 0.1% dietary ${\beta}$-glucan(P<0.05). In conclusion, dietary ${\beta}$-glucan reduced adiposity and improved serum lipids in obese rats fed high fat diet. The present study suggest that ${\beta}$-glucan supplementation to the diet is beneficial in suppressing diet-induced obesity and dyslipidemia.

Experimental Assessment of Hemostatic Agents: Comparison with New Developed Chitosan-Based Material (신개발 키토산 제재의 지혈 효과에 대한 비교)

  • Cho, Young-Kyoo;Lee, Sang-Yun;Kim, Tae-Jung;Lim, Hyun-Ju;Oh, Eun-Jung;Lee, Soo-Bok;Choi, Kang-Young;Yang, Jung-Dug;Cho, Byung-Chae;Chung, Ho-Yun
    • Archives of Plastic Surgery
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    • v.38 no.4
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    • pp.369-375
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    • 2011
  • Purpose: Many hemostatic agents and dressings have been tested with variable degree of success. Chitosan has a positive charge, it attracts red blood cells, which have a negative charge. Our goal is to test the efficacy of new developed chitosan-based hemostatic materials in providing durable hemostasis in a high-flow arterial wound model. Methods: We compared each group with SD rats motality tests and in vitro blood compatibility test by blood clotting index (BCI). We devided the SD rats into 6 groups (N =15) by type of hemostatic agents. A: 100% nonwoven chitosan (degree of the deacetylation: 90%). B: 50% N-acetylation on nonwoven of chitosan gel (degree of the deacetylation: 50%). C: 60% N-acetylation on nonwoven of chitosan ge (degree of the deacetylation: 40%)l. D: Cutanplast$^{(R)}$. E: HemCon$^{(R)}$ F: Gauze. In vivo test, a proximal arterial injury was created in unilateral femoral arteries of 90 anesthetized SD rats. Each materials was made same size and thickness then applied to the injury site for 3 minutes. In vitro test, we compared each group with BCI in human blood. Results: In vivo test, group A showed lower motality rate of 46% than any other groups, Group B and C showed lower motality rate of 60% than group D and E's motality rate of 66%. In vitro test, BCI of group A ($30.6{\pm}1.2$) and B ($29.3{\pm}1.0$) were showed nearly about group D ($29.1{\pm}1.8$) and E ($27.4{\pm}1.6$). Group C ($37.1{\pm}2.0$) showed higher BCI than group A and B, it means group C decreased blood clotting. Conclusion: In conclusion, this study suggests a newly developed chitosan-based hemostatic materials induced durable hemostasis and increased blood clotting, and are considered as effective biologic hemostatic agents.

The Effect of Dachaihu Decoction for Hyperlipidemic Acute Pancreatitis: A Systematic Review and Meta-Analysis (고지혈성 급성 췌장염에 대한 대시호탕의 효과 : 체계적 문헌고찰과 메타분석)

  • Kim, Yoon-jung;Jung, Yu-jin;Park, Dong-il
    • The Journal of Internal Korean Medicine
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    • v.41 no.3
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    • pp.306-325
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    • 2020
  • Objectives: The aim of this study is to investigate the effect of a Dachaihu decoction for hyperlipidemic acute pancreatitis (HLAP) by systematic review and meta-analysis of Chinese clinical studies. Methods: China National Knowledge Infrastructure (CNKI) was utilized as the major search engine. The date of the literature search was March 7, 2020. Randomized controlled trials (RCTs) about using a Dachaihu decoction for HLAP were included in this study. Meta-analysis was performed by synthesizing outcome data, including total effective rate, abdomen pain relief time, first bowel movement time, blood amylase recovery time, and triglyceride (TG) levels (mmol/L). The selected literature was assessed using Cochrane's risk of bias (RoB). Results: Twelve of 44 RCTs met the inclusion criteria. Most studies were evaluated with RoB as having unclear risk. The total effective rate of herbal medicine treatment based on the Dachaihu decoction was significantly higher than that of symptomatic supportive treatment in 10 articles (risk ratio=1.15, 95% CI: 1.08 to 1.21, p<0.00001, I2=0%). Herbal medicine treatment based on a Dachaihu decoction was significantly more effective than symptomatic supportive treatment in terms of reducing abdomen pain relief time (in all articles; mean difference=-1.70, 95% CI: -1.91 to -1.41, p<0.00001, I2=45%), first bowel movement time (in 7 articles; mean difference=-1.46, 95% CI: -1.86 to -1.05, p<0.00001, I2=73%), blood amylase recovery time (in 8 articles; mean difference=-1.48, 95% CI: -2.04 to -0.92, p<0.00001, I2=90%), and TG levels (in 8 articles; mean difference=-1.59, 95% CI: -2.28to -0.91, p<0.00001, I2=90%). Only one article reported side effects of treatment among the intervention group and control group, citing pancreatic ulcer and pancreatic pseudocyst formation. Conclusions: This study suggests that herbal medicine treatment based on a Dachaihu decoction could yield higher efficacy for HLAP than symptomatic supportive treatment alone. However, the results might be somewhat biased because of the poor quality and small sample size of the included RCTs. Well-qualified clinical studies are needed to prove the effectiveness of Dachaihu decoction therapy for HLAP.

Hypolipidemic and Anti-oxidant Effects of Chunghyl Plus in Type II Diabetic Mice Model (제2형 당뇨 마우스 모델에서 청혈플러스의 항고지혈 및 항산화효과)

  • Choi, Koh Eun;Seol, In Chan;Kim, Yoon Sik;Cho, Hyun Kyoung;Yoo, Ho Ryong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.30 no.3
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    • pp.164-176
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    • 2016
  • This study was perfomed to investigate the effects of Chunghyul-plus(CHP) on oxidative damage and hyperlipidemia in db/db mouse. After treatment with CHP, safety in cytotoxicity, heavy metal toxicity, production of reactive oxygen species(ROS), nitric oxide (N0) and proinflammatory cytokine IL-Ib, TNF-a, IL-6 in RAW 264.7 cells. Serum total cholesterol, LDL cholesterol, HDL cholesterol, triglyceride, insulin, GLP-1, glucose, food intake, body weight, organ weight, AST, ALT, ALP, BUN, creatine and histologic change of liver and aorta were measured in db/db mouse after oral administration of CHP. CHP showed safety in cytotoxicity and toxicity of liver and kidney for logn time administration. CHP increased the DPPH and ABTS radical scavenging activity. CHP showed significant inhibitory effect on reactive oxygen species (ROS), and showed inhibitory effect on nitiric oxide(NO) compared to control group. CHP decreased cytokine IL-6 production significantly, and decreased IL-1β and TNF-α compared to control group. CHP decreased body and organ weitht, intake food, and glucose levels compared to control group. CHP decreased total cholesterol and triglyceride significantly, and decreased LDL-cholesterol levels and increased HDL-cholesterol levels compared to control group. CHP decreased atherogenic index and cardiac risk factor significantly. CHP increased serum insulin and GLP-1 compared to control group. In histologic examination, lipophagy in the liver and aorta decreased in CHP treated mice and the cell was regular and boundary of vessel wall was clear compared to control group. These results suggest that CHP is effective in antioxidation activity and treatment and prevention of hyperlipidemia, atherosclerosis, diabetes, ischemic heart disease, stroke and other cardiocerebrovascular disease.

Anticarcinogenic Effects of Allium tuberosum on Human Cancer Cells (부추 추출 성분의 항발암 효과 연구)

  • Park, Yun-Ja;Kim, Mi-Hyang;Bae, Song-Ja
    • Korean Journal of Food Science and Technology
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    • v.34 no.4
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    • pp.688-693
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    • 2002
  • The anticarcinogenic effects of various food components have received much attention in recent years. However mechanism of anticarcinogens in food materials on cancer cells have rarely been investigated. This study was performed to investigate the effects on the cytotoxicity and quinone reductase (QR) activity of Allium tuberusum (AT) on the human cancer cells. The six partition layers which are methanol (ATM), hexane (ATMH), ethylether (ATMEE), ethylacetate (ATMEA), butaonl (ATMB) and aqueous (ATMA) of Allium tuberusum were screened for their cytotoxic effects on HepG2, MCF-7, HeLa and SK-N-MC cells by the MTT assay. Among the six partition layers, ATMEE had the strongest cytotoxic effect at concentration of $150\;{\mu}g/mL$ which resulted over 95% on HepG2, HeLa, MCF-7 and SK-N-MC cell lines. The ATMEA also showed significant cytotoxic effect on HepG2 and SK-N-MC cell lines. The ATMB showed the highest induction activity of QR on HepG2 cells among the other partition layers. QR activity of HepG2 cells, grown in the presence of ATMB at the concentration of $50\;{\mu}g/mL$, was increased by 3.9 times, compared to the control value of 1.0. Based on these results, the ATMEE and ATMB may have potentially anticarcinogenic and chemopreventive activities.

Surgical Experience of Paraclinoidal Aneurysms (상상돌기 주위 동맥류의 수술적 치험)

  • Kang, Hyung Gon;Jo, Chul Min;Huh, Jae Teack
    • Journal of Korean Neurosurgical Society
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    • v.30 no.sup2
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    • pp.203-210
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    • 2001
  • Objective : Paraclinoidal aneurysms termed that aneurysms arising from proximal internal carotid artery(ICA) between the site of emergence of the carotid artery from roof of the cavernous sinus and origin of the posterior communicating artery(PCoA). These aneurysms pose conceptual and technical surgical problems with regard to acquisition of proximal control and safe intracranial exposure. The efficiency of surgical technique according to the location of paraclinoidal aneurysm was studied for minimal exposure. Materials and Methods : Over the past four years, the authors treated surgically 171 cases of cerebral aneurysm, among them ten patients were paraclinoidal aneurysms with two patients unruptured aneurysms. Mean age was 47 years old, and all patients were female. Three patients were proximal posterior carotid artery wall aneurysms(one large, one giant), four patients carotid-ophthalmic artery aneurysms and three patients superior hypophyseal artery aneurysms. Results : There could be done clip in all cases, there were no deaths and no complication. And no patient developed sustained neurological deficits including visual function except hydrocephalus in one case. Four patients complained of visual disturbance but two patients had recovery after postoperation and two patient were not longer to bad. Conclusion : Our recent experience suggests that preoperative scrutiny of diagnostic angiography allows classification of all paraclinoidal aneurysms regardless of size and surgical technique which this classification has focused on operative approaches unique to each aneurysm projection was helpful to improve the operative outcome with good visual function and to shorten the operative time.

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Pot Cultivation Method of Veronica rotunda Nakai (둥근산꼬리풀의 포트 재배 방법)

  • Yeon, Soo Ho;Lee, Seung Yeon;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.88-88
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    • 2019
  • 현삼과(Scrophulariaceae)의 둥근산꼬리풀(Veronica rotunda Nakai)은 흰색의 꽃이 총상화서로 피며, 관상용으로 쓰인다. 또한, 접선골도라 하여, 전초를 약용하고 활혈, 지혈, 청폐열, 화간위에 효능이 있는 자생식물이다. 본 연구는 둥근산꼬리풀의 포트 재배방법 확립을 위하여 2018년 5월 15일에 파종하여 생산된 유묘를 7월 16일에 정식하여 10월 15일까지 약 14주 동안 수행하였다. 식재용기 실험은 비닐포트[3치($7{\times}7cm$), 4치($9{\times}8.5cm$)]와 플라스틱 화분[8호($7{\times}7cm$), 10호($9{\times}9cm$), 12호($10.5{\times}10.5cm$)]를 이용하였고, 토양 종류[중화 피트모스:펄라이트(3:1, 4:1), 마사토:중화 피트모스(2:1, 3:1)], 묘의 소질[파종 립수(1, 2, 4, 6립), 파종 용기(162, 200, 288구 트레이)] 등의 연구를 진행하였다. 대조구는 플라스틱 화분 10호에 원예상토를 충진하고 200구 트레이에 4립씩 파종하여 생산된 1셀을 정식하였다. 연구의 결과, 식재용기에 따른 둥근산꼬리풀의 생육은 플라스틱 12호 화분에서 가장 양호하였으며, 토양의 양이 많을수록 전반적인 생육이 증가하였다. 토양 종류별로는 원예상토에서 재배한 처리구에서 양호한 생육을 보였다. 중화 피트모스와 펄라이트 3:1 혼용토의 경우, 원예상토와 전반적인 생육에 큰 차이가 없어 이용가치가 있을 것으로 판단된다. 한편, 피트모스의 비율이 높은 4:1 혼용토에서는 비교적 생육이 억제되었으며, 잎이 황화되었다. 마사토와 중화 피트모스의 혼용토는 원예상토에 비해 왜화되는 결과를 보였으며, 측지수, 마디수, 엽수에는 큰 영향을 주지 않았다. 그러나 개화율을 감소시키는 결과를 보였다. 셀당 파종한 종자의 양을 달리하여 생산된 유묘를 정식하여 재배한 결과, 전반적인 생육은 1립 파종구에서 양호하였다. 한편, 파종량이 많을수록 초장, 절간장이 감소하며, 포트당 마디수, 측지수 및 개화율이 증가하여, 본 연구의 목표인 관상적 이용에 적합한 것으로 판단된다. 파종용기별 생산된 유묘이식 결과, 초기생육이 왕성할수록 후기생육도 우수하였다. 162구 생산묘 정식구는 다른 처리구보다 전반적으로 생육이 왕성하였으며, 다음으로 200구 순이었다. 파종용기에 따른 묘의 크기는 정식 후의 개화율에 유의적인 차이가 없었다. 따라서, 둥근산꼬리풀의 재배는 플라스틱 화분 12호에 원예상토를 충진하여 162구 트레이에 셀당 6립 파종하여 생산된 유묘를 정식하는 것이 분화용 재배에 효과적인 것으로 생각된다. 한편, 재배에 이용되는 토양의 종류에 따른 비율 및 개화촉진 등의 추가적인 연구가 필요하다.

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Natural Origin Polymers: Applications as Wound Care Materials (자연 고분자 : 상처 치료 재료로 활용)

  • Karadeniz, Fatih;Sung, Hye Kyeong;Kim, Han Seong
    • Journal of Life Science
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    • v.29 no.3
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    • pp.382-393
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    • 2019
  • Wound care is a health industry concern affecting millions worldwide. Recent increase in metabolic disorders such as diabetes comes with elevated risk of wound-based complications. Treatment and management of wounds are difficult practices due to complexity of the wound healing process. Conventional wound dressings and treatment applications only provide limited benefits which are mainly aimed to keep wound protected from external factors. To improve wound care, recent developments make biopolymers to be of high interest and importance to researchers and medical practitioners. Biopolymers are polymers or natural origin produced by living organisms. They are credited to be highly biocompatible and biodegradable. Currently, studies reported biopolymers to exhibit various health beneficial properties such as antimicrobial, anti-inflammatory, hemostatic, cell proliferative and angiogenic activities which are crucial for effective wound management. Several biopolymers, namely chitosan, cellulose, collagen, hyaluronic acid and alginic acid have been already investigated and applied as wound dressing agents. Different derivatives of biopolymers have also been developed by cross-linking with other molecules, grafting with other polymers, and loading with bioactive agents or drugs which showed promising results towards wound healing without any undesired outcome such as scarring and physiological abnormalities. In this review, current applications of common biopolymers in wound treatment industry are highlighted to be a guide for further applications and studies.

Inhibitory Effects of Scopoletin in Collagen-induced Human Platelet Aggregation (콜라겐으로 유도한 사람 혈소판 응집에 미치는 Scopoletin의 억제 효과)

  • Kwon, Hyuk-Woo;Shin, Jung-Hae;Park, Chang-Eun;Lee, Dong-Ha
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.1
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    • pp.34-41
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    • 2019
  • Platelet aggregation is essential for the formation of a hemostatic plug in the case of blood vessel damage. On the other hand, excessive platelet aggregation may cause cardiovascular disorders, such as thrombosis, atherosclerosis, and myocardial infarction. Scopoletin, which found in the root of plants in the genus Scopolia or Artemisia, has anti-coagulation and anti-malaria effects. This study examined the effects of scopoletin on human platelet aggregation induced by collagen. Scopoletin had anti-platelet effects via the down-regulation of thromboxane $A_2$ ($TXA_2$) production and intracellular $Ca^{2+}$ mobilization ($[Ca^{2+}]_i$), which are aggregation-inducing molecules produced in activated platelets. On the other hand, scopoletin increased both the cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels, which are known as intracellular $Ca^{2+}$-antagonists and aggregation-inhibiting molecules. In particular, scopoletin increased the potently cAMP level more than cGMP, which led to suppressed fibrinogen binding to ${\alpha}IIb/{\beta}_3$ in collagen-induced human platelet aggregation. In addition, scopoletin inhibited collagen-elevated adenosine triphosphate (ATP) release in a dose-dependent manner. The results suggest that aggregation amplification through granule secretion is inhibited by scopoletin. Therefore, scopoletin has potent anti-platelet effects and may have potential for the prevention of platelet-derived vascular diseases.

Maintenance of Platelet Counts with Low Level QC Materials and the Change in P-LCR according to Hemolysis with XN-9000 (XN-9000장비에서 Low Level QC물질에서의 혈소판 수 관리와 용혈에 따른 P-LCR의 변화)

  • Shim, Moon-Jung;Lee, Hyun-A
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.4
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    • pp.399-405
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    • 2018
  • The platelet count in clinical laboratories is essential for the diagnosis and treatment of hemostasis abnormalities, and accurate platelet counting in the low count range is of prime importance for deciding if a platelet transfusion is needed and for monitoring after chemotherapy. Quality control is designed to reduce and correct any deficiencies in the internal analytical process of a clinical laboratory prior to the release of patient results. Fragmented erythrocytes are the major confusing factors for platelet counting because of their similar size to platelets. The authors found that the low range QC values were out of 2SD with a Sysmex automatic analyzer in internal quality control process. Thus far, there has been little discussion on the relationship between hemolysis and the platelet parameters. Therefore, this study focused on the performance of automated platelet counts, including the PLT-F, the PLT-I, and PLT-O methods at the low platelet range using the low level QC materials and compared the 5 platelet parameters with the hemolyzed samples. The results showed that the CV was the smallest with PLT-F and P-LCR increased from 18.4 to 31.9% in the hemolysis samples. These results indicate that a more accurate estimation of the platelet counts can be achieved using the PLT-F method than the PLT-I method at the low platelet range. The use of the PLT-F system improves the confidence of results in low platelets samples in a routine hematology laboratory. The results suggest that P-LCR is a new parameter in assessing samples when the specimen is suspected of hemolysis and deterioration. Nevertheless, further studies will be needed to establish the relationship with P-LCR and hemolysis using human blood specimens.