• Korean Journal of Food Science and Technology
• /
• v.18 no.2
• /
• pp.118-123
• /
• 1986

Soy milk prepared from soy protein concentrate was fermented with Lactobacillus acidophilus, L. bulgaricus, L. casci, Streptococcus lactis or S. cremoris. Growth and acid production of each organism in soy milk and flavor of soy yogurt beverages were investigated. Volatile compounds in soy milk and soy yogurts were also determined. Among the five organisms tested, L. bulgaricus produced most amount of acid in soy milk while S. cremoris produced least amount of acid in soy mile. Sensory evaluation showed that the flavor of soy yogurt beverages was inferior to that of milk yogurt beverage and the flavor of soy yogurt beverage prepared by L. bulgaricus was better than that of other soy yogurt beverages. Soy milk fermented with L. bulgaricus was more acceptable than unfermented soy milk. Lactic fermentation reduced n-hexanal in soy milk while it produced diacetyl that was not detected in unfermented soy milk.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2003.10a
• /
• pp.216.2-216
• /
• 2003

본 연구에서는 우유에 탈지분유 또는 저장기간이 다른 3종의 통일벼(86년산, 88년산, 89년산)를 각각 2%(w/v)첨가하고 젖산균(Lacttobacillus 4종, Leuconostoc 1종)으로 발효하여 커드사으이 요구르트를 만든 후, 저장기잔이 다른 쌀의 첨가가 젖산균의 산생성과 커드상 요구르트의 품질(관능성, 점도, 저장성)에 미치는 영향을 조사하였다. TKf의 첨가로 젖산균의 산생성이 대조군(우유로만 만든 요구르트)보다 촉진되었는데, 젖산균의 종에 따라 다소 차이는 있으나 싸렝 의한 젖산균의 상생성 촉진효과는 쌀의 저장기간에 의하여 현저한 영향을 받지 않았다. 관능검사의 결과를 보면 우유에 첨가된 쌀은 저장기간이 다르더라도 요구르트의 관능성에 현저한 영향을 미치지 않았다. 점도측정의 결과를 보면 쌀의 저장기간이 경과할수록 쌀 첨가 요구르트의 첨도가 증가하였으며, 커드상 요구르트는 thixotropic fiow의 특성을 나타냈다. 5$^{\circ}C$에서 15일간 보존된 쌀 첨가요구르트의 저장성은 첨가된 쌀의 저장기간에 관계없이 우수하였다.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2003.10a
• /
• pp.216.3-217
• /
• 2003

본 연구에서는 우유에 탈지분유 또는 4종의 쌀을 각각 2%(w/v)첨가하여 커드상의 요구르트를 만들고, 대조군(우유로만 만든 요구르트)과 비교하여 쌀의 첨가가 젖산균의 산생성 및 커드상 요구르트의 품질(관능성, 저장성에 미치는 영향을 조사하였으며, HPLC를 이용하여 몇 가지 유기산을 분석하였다. 쌀의 첨가로 젖산균의 산생성이 대조군보다 촉진되었는데 현미의 촉진효과가 가장 현저하였다. 접종된 4종의 젖산균(Lacttobacillus 3종, Leuconostoc 1종) 중에서는 Lenc. mesenteroides 와 L. bulgaricus 의 산생성도가 높았다. HPLC에 의한 유기산 분석의 결과를 보면 우유나 2% 현미를 첨가한 우유에 L. bulgaricus를 접종하여 24시간 발효하는 동안 lactic acid 는 젖산균에 의해 생성되어 그 양이 현저하게 증가하였으나, 우유 중에 들어있는 citric acid 는 젖산균에 의해 이용되어 그 양이 현저히 감소하였다. 커드상 요구르트의 관능성은 쌀의 첨가로 표준시료보다 다소 저하되었으며, 쌀 첨가시료 중에서는 통일벼 참가시료가 다른 시료보다 우수하였다. 커드상의 요구르트를 2주일 간 5$^{\circ}C$냉장고에 저장하는 동안 산생성과 pH, 생균수에 거의 변화가 없어 저장성은 매우 우수한 것으로 나타났다.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.34 no.1
• /
• pp.33-38
• /
• 2014

To provide the basic data on the utilization of chlorella as a method to improve the quality of forage, we examined the effect of the addition of lactic acid bacteria (LAB) and chlorella on the change of nutritive value, quality and microbes on Italian ryegrass and alfalfa mixture silage (IRG-Alfalfa silage). The nutritive values of IRG-Alfalfa silage were not significantly different in all treatments. The pH of IRG-Alfalfa silage decreased in both LAB and chlorella treatments. Further the content of lactic acid in the LAB treatment increased as compared to the control, and also significantly increased in the treatment of LAB with chlorella (p<0.05). In addition, the number of LAB increased in lactic acid bacteria treatment as compared to the control, and also increased in the treatment of LAB with chlorella (p<0.05). Therefore, we suggest that LAB and chlorella would improve the quality of IRG-Alfalfa silage.

• The Korean Journal of Food And Nutrition
• /
• v.10 no.3
• /
• pp.350-359
• /
• 1997

The changes in pH, acid contents and microbial counts were investigated during fermentation of Baikkimchi, a kind of Kimchi without red pepper, and the major microbial groups were also isolated and identified. Immediately after the preparation of Baikkimchi(pH 6.15, acid contents 0.03%), its major microbial group was Gram negative rods, and was composed of Pseudomonas(55%), Enterobacter(40%) and Erwinia(5%). After 2 days of fermentation at 15$^{\circ}C$, the most predominant microbial group was changed to lactic acid bacteria. Lactic acid bacteria showed 1st, 2nd and 3rd stationary phase on its growth curve in 4, 12 and 50 days of fermentation, respectively. At the 2nd stationary phase of lactic acid bacteria(pH 3.51, acid contents 0.59%), the group was composed of Lactobacillus bavaricus(55%), Leuconostoc mesenteroides subsp. mesenteroides(42.5%) and Leuconostoc paramesenteroides(2.5%), while at the 3rd stationary phase(pH 3.40, acid contents 1.10%), that was Lactobacillus plantarum(65%) and Lactobacillus brevis(35%). The physiological and biochemical characteristics identified as Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc paramesenteroides, Lactobacillus plantarum and Lactobacillus brevis showed good agreement with the current taxonomic system, but those identified as Lactobacillus bavaricus showed some disagreements. The number of yeast was decreased wit the increase in the number of lactic acid bacteria. Yeast showed stationary phase in 30 days between the 2nd and 3rd stationary phase of lactic acid bacteria, and the group was composed of only gunus Saccharomyces.

• The Korean Journal of Food And Nutrition
• /
• v.23 no.4
• /
• pp.664-669
• /
• 2010

Lactic acid bacteria from traditional Korean foods of Tarak and Kimchi was isolated and characterized against carcinogenic Helicobacter pylori. Five Tarak and 30 Kimchi, traditional lactic acid-fermented foods, were collected from Andong area and the markets in Seoul, respectively and 15 lactic acid bacteria were isolated. Among them, two isolates were selected from high growth-inhibitory activities on H. pylori. The isolates were identified as Streptococcus thermophilus LAB kw15 from Tarak and Leuconostoc mesenteroides LAB kw5 from Kimchi by the biochemical characteristics and 16S DNA sequencing. The culture solutions of the isolates adjusted to pH 7.0 showed H. pylori inhibition. The isolates grew well and H. pylori did not grow during the co-culture with those strains. Therefore, L. mesenteroides LAB kw5 and S. thermophilus LAB kw15 might be the candidates as the functional lactic acid bacteria for improving stomach health.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.21 no.6
• /
• pp.681-685
• /
• 1992

Low molecular weight RNA(LMW RNA : 5S rRNA and tRNAs, <150 nucleotides) profiles of several bacteriocin production lactic acid bacteria from pig meats and reference lactic acid bacteria were generated on 10% denaturing polyacrylamide gel electrophoresis. Data evaluation including three molecular weight markers enabled the calculation of relative nucleotide units(RNU) for every band. Gels profiles and RNU evaluations were effective for identification of lactic acid bacteria species. LMW RNA profiles of lactic acid bacteria showed no variation in dependence on APT(All Purpose Tryptone Broth), TSB(Tryptic Soy Broth), MRS(Lactobacilli MRS Broth) different cultural medium.

• Food Science of Animal Resources
• /
• v.24 no.2
• /
• pp.164-170
• /
• 2004

The purpose of this study is to isolate probiotic lactic acid bacteria (LAB) that produced bile salts hydrolase. One hundred twenty strains were initially isolated from human feces. Based on their resistance of acid, tolerances of bile salts, and inhibitory activity against Escherichia coli, five strains were selected. A strain producing highest activity of bile salts hydrolase was identified as Lactoacillus plantarum using API carbohydrate fermentation pattern and 16S rRNA sequences, and named CK102. Lactobacillus plantarum CK102 survived at a level of 1.36${\times}$10$\^$8/ CFU/$m\ell$ in pH 2 buffer for 6 h and showed exhibited excellent bile tolerance. When L plantarum CK102 was cultured with E. coli in MRS broth, no viable cells of E. coli was detected after 18 h fermentation. These results suggest that Lactobacillus plantarum CK 102 may be commercially used for the probiotic culture.

• Applied Biological Chemistry
• /
• v.40 no.6
• /
• pp.541-545
• /
• 1997

This study was conducted to investigate the effect of cocoon silk fibroin hydrolyzate(CSFH) on shelf-life extension of kimchi. CSFH was added in kimchi in the concentration of 0.3%(w/w), 0.6% and 0.9%. Kimchi with CSFH showed higher pH and lower titratable acidity than control and it was supposed that the growth of lactic acid bacteria was inhibited by CSFH. In model system, for complex lactic acid bacteria, CSFH showes antimicrobial activity.

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 1975.12a
• /
• pp.181.4-181
• /
• 1975

유포자 젖산균 Bacillus sp의 식품이용을 위한 기초로서 유포자 젖산균의 발육, 포자형성 등에 관하여 연구한 결과는 다음과 같다. 탄수화물 중 dextrin이 좋았고, Mn, Ca ion, biotin 등은 생육, 포자형성, 내열성에 대하여 효과가 좋았다. 배양의 최적조건은 호기조건하에서 $37~45^{\circ}C에서$ 36시간 이상 배양하는 것이었다.