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Isolation and Identification of Lactic Bacteria Containing Superior Activity of the Bile Salts Deconjugation  

하철규 (한양대학교 생화학 및 분자생물학과)
조진국 (국립한경대학교 낙농과학과)
채영규 (한양대학교 생화학 및 분자생물학과)
허강칠 (국립한경대학교 낙농과학과, (주) 빅바이오젠)
Publication Information
Food Science of Animal Resources / v.24, no.2, 2004 , pp. 164-170 More about this Journal
Abstract
The purpose of this study is to isolate probiotic lactic acid bacteria (LAB) that produced bile salts hydrolase. One hundred twenty strains were initially isolated from human feces. Based on their resistance of acid, tolerances of bile salts, and inhibitory activity against Escherichia coli, five strains were selected. A strain producing highest activity of bile salts hydrolase was identified as Lactoacillus plantarum using API carbohydrate fermentation pattern and 16S rRNA sequences, and named CK102. Lactobacillus plantarum CK102 survived at a level of 1.36${\times}$10$\^$8/ CFU/$m\ell$ in pH 2 buffer for 6 h and showed exhibited excellent bile tolerance. When L plantarum CK102 was cultured with E. coli in MRS broth, no viable cells of E. coli was detected after 18 h fermentation. These results suggest that Lactobacillus plantarum CK 102 may be commercially used for the probiotic culture.
Keywords
bile salts; lactic acid bacteria; deconjugation; isolation and identification;
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