Antimicrobial Effect of Lactic Acid Bacteria Isolated from Kimchi and Tarak on Helicobacter pylori

김치와 타락에서 분리한 젖산균의 Helicobacterpylori에 대한 항균 효과

  • Lee, Young-Duck (Dept. of Food Science and Biotechnology, Kyungwon University) ;
  • Yoo, Hye-Lim (Dept. of Food Science and Biotechnology, Kyungwon University) ;
  • Hwang, Ji-Yeon (Research and Development Department, BKbio Co. Ltd.) ;
  • Han, Bok-Kyung (Research and Development Department, BKbio Co. Ltd.) ;
  • Choi, Hyuk-Joon (*Research and Development Department, BKbio Co. Ltd.) ;
  • Park, Jong-Hyun (Dept. of Food Science and Biotechnology, Kyungwon University)
  • Received : 2010.11.20
  • Accepted : 2010.12.18
  • Published : 2010.12.01

Abstract

Lactic acid bacteria from traditional Korean foods of Tarak and Kimchi was isolated and characterized against carcinogenic Helicobacter pylori. Five Tarak and 30 Kimchi, traditional lactic acid-fermented foods, were collected from Andong area and the markets in Seoul, respectively and 15 lactic acid bacteria were isolated. Among them, two isolates were selected from high growth-inhibitory activities on H. pylori. The isolates were identified as Streptococcus thermophilus LAB kw15 from Tarak and Leuconostoc mesenteroides LAB kw5 from Kimchi by the biochemical characteristics and 16S DNA sequencing. The culture solutions of the isolates adjusted to pH 7.0 showed H. pylori inhibition. The isolates grew well and H. pylori did not grow during the co-culture with those strains. Therefore, L. mesenteroides LAB kw5 and S. thermophilus LAB kw15 might be the candidates as the functional lactic acid bacteria for improving stomach health.

한국인의 주된 위 건강의 저해인자인 Helicobacter pylori를 저해하는 젖산균을 타락과 김치에서 분리하여 특성을 분석하고자 하였다. 전통발효 젖산균음료인 타락 5개와 김치 30종을 안동 지역과 수도권의 시장에서 구입하고, 젖산균을 전통적인 분리방법에 따라 15주를 분리하였다. 이들 젖산균에 대하여 H. pylori 생육 저해 활성이 높은 두 개의 젖산균을 선택하여 동정하였다. 이들 젖산균은 전형적인 생화학적인 특성과 16S DNA의 분석을 통하여 타락에서 분리된 균은 Streptococcus thermophilus LAB kw15로 김치에서 분리된 균은 Leuconostoc mesenteroides LAB kw5로 동정하였다. 이들 분리 균주 배양액을 중성으로 조정한 후 첨가하여 배양할 때 H. pylori의 생육을 현저히 저해하였으며, H. pylori와의 혼합 배양에서도 H. pylori의 생육을 저해하는 것으로 나타났다. 그러므로 이 분리 젖산균의 대사산물이 H. pylori의 생육을 저해하고 있는 것으로 나타나, 기능성 젖산균으로의 활용이 가능할 것으로 판단된다.

Keywords

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