• Title/Summary/Keyword: 정자수

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A Study on the Morphological Analysis of Sperm (정자의 형태학적 특성 분석에 관한 연구)

  • Paick, Jae-Seung;Jeon, Seong-Soo;Kim, Soo-Woong;Yi, Won-Jin;Park, Kwang-Suk
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.2
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    • pp.153-165
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    • 1997
  • In male reproducible health, fertility and IVF (in-vitro fertilization), semen analysis has been most important. Semen analysis can be divided into concentration, motional and morphological analysis of sperm. The existing method which was developed earlier to analyze semen concentrated on the sperm motility analysis. To provide more useful and precise solutions for clinical problems such as infertility, semen analysis must include sperm morphological analysis. But the traditional tools for semen analysis are subjective, imprecise, inaccurate, difficult to standardize, and difficult to reproduce. Therefore, with the help of development of microcomputers and image processing techniques, we developed a new sperm morphology analyzer to overcome these problems. In this study the agreement on percent normal morphology was studied between different observers and a computerized sperm morphology analyzer on a slide-by-slide basis using strict criteria. Slides from 30 different patients from the SNUH andrology laboratory were selected randomly. Microscopic fields and sperm cells were chosen randomly and percent normal morphology was recorded. The ability of sperm morphology analyzer to repeat the same reading for normal and abnormal cells was studied. The results showed that there was no significant bias between two experienced observers. The limits of agreement were 4.1%${\sim}$-3.8%. The Pearson correlation coefficient between readers was 0.79. Between the manual and sperm morphology analyzer, the same findings were reported. In this experiments the slides were stained by two different methods, PAP and Diff-Quik staining methods. The limits of agreement were 7.2%${\sim}$-5.7% and 6.0%${\sim}$-6.3%, respectively. The Pearson correlation coefficients ware 0.76 and 0.91, respectively. The limits of agreement was tighter below 20% normal forms. In the experiments of repeatability, 52 cells stained by PAP and Diff-Quik staining methods were analyzed three times in succession. Estimating pairwise agreement, the kappa statistic for the pairs were 0.76, 0.81, 0.86, and 0.75, 0.88, 0.88 respectively. In this study it was shown that there was good agreement between manual and computerized assessment of normal and abnormal cells. The repeatability and agreement per slide of computerized sperm morphology analyzer was excellent. The computer's ability to classify normal morphology per slide is promising. Based on results obtained, this system can be of clinical value both in andrology laboratories and IVF units.

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Antimicrobial activity and protective effect of Geranium thunbergii against oxidative DNA damage via antioxidant effect (현초의 항산화 활성에 의한 산화적 DNA 손상 보호효과 및 항균활성)

  • Kwon, Tae-Hyung;Lee, Su-Jin;Park, Jae-Ho;Kim, Taewan;Park, Jung-Ja;Park, Nyun-Ho
    • Food Science and Preservation
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    • v.24 no.3
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    • pp.325-333
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    • 2017
  • This study aimed to investigate the various biological activities of Geranium thunbergii such as antimicrobial activity and protective effect against oxidative damage. To evaluate its antioxidant and antimicrobial activities, we first performed methanol extraction; this methanol extract was further partitioned using various solvents. And then, its antioxidant activity was measured using various assays including total phenolic content and protection against oxidative DNA damage, and antimicrobial activities were examined using minimum inhibiting concentration (MIC) test, and paper disc method. In addition, high-performance liquid chromatography was performed to analyze the major chemical components of ethyl acetate fraction. The G. thunbergii fraction with ethyl acetate exhibited higher antioxidant and antimicrobial activities than the other fractions. The results showed that G. thunbergii ethyl acetate fraction at $50{\mu}g/mL$ had strong DPPH and ABTS radical scavenging activities of 80.88% and 80.12%, respectively. In addition, the ethyl acetate fraction protected DNA from the oxidative damage induced by ferrous ion and hydroxyl radicals and showed high antimicrobial activity with diameter of inhibition zones ranging from 13.33 to 15.67 mm. High-performance liquid chromatography analysis revealed the major phenolic compounds of G. thunbergii to be ellagic acid and gallic acid. These results suggest that G. thunbergii might protect DNA against oxidative stress induced by reactive oxygen species and can be utilized as a natural source of antioxidant and antimicrobial agent in the food industry.

Components and Biological Effects of Fermented Extract from Tartary Buckwheat Sprouts (타타리메밀싹 발효추출물의 영양성분 및 생리활성)

  • Chang, Kwang-Jin;Seo, Geon-Sik;Kim, Yang-Sik;Huang, Dae-Sun;Park, Jong-In;Park, Jeong-Ja;Lim, Yong-Sup;Park, Byoung-Jae;Park, Cheol-Ho;Lee, Man-Hee
    • Korean Journal of Plant Resources
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    • v.23 no.2
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    • pp.131-137
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    • 2010
  • Tartary buckwheat has potential as a source of functional food because it contains a number of bioactive compounds such as rutin, catechin and so on. This study was conducted to determine the possibility of development of processed products extracted from sugar-treated sprouts of tartary buckwheat. By using undiluted solution extracted from sprouts of tartary buckwheat, we analyzed their nutrition components and did in vivo experiment to find out pharmaceutical effects. In an experiment using mice, we administered various concentration of buckwheat to induced diabetic mellitus mice for 1 weeks. As a result, the fermented extract from buckwheat sprouts effected finely on lowering blood sugar and decreased LDL-cholesterol and total lipid level but increased HDL-cholesterol level.

The effect of a soil amendment on phosphate efficiency in a low productive paddy soil (저위생산답(低位生産沓) 토양(土壤)에 대(對)한 개량제(改良劑)와 인산(燐酸)의 효과(效果))

  • Shim, Sang Chil;Song, Ki Joon;Kim, Chung Ja
    • Korean Journal of Soil Science and Fertilizer
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    • v.4 no.1
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    • pp.21-26
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    • 1971
  • Present work is concerned with the effects of a soil amendment (a mixture of basic slag with trace elements Cu, Mn, Zn and B) on the phosphate uptake by rice plants and in improving yield of rice in low productive paddy soils. The experiment was conducted at Kimpo-myun, Kimpo-kun, Kyunggi province which is characterized as "Akiochi" area and split plot experimental design was adapted. The results are summarized as follows; 1. Combined effect of the soil amendment and phosphate applications on the grain yield is pronounced, which is also characterized by the increased grain weight, maturing rate and seed setting rate. 2. Treatment of soil amendment appears to improve phosphate efficiency; grain weight, maturing rate and seed setting rate are all improved as the rate of phosphate application increased. 3. Phosphate tends to accelerate plant growth at earlier stages of plant development while the soil amendment retards the growth, inhibiting excessive tillering. 4. The soil amendment increases silicate and manganese, but decreases phosphate, copper and Zinc contents in the rice plants.

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A Study on the Architectural Characteristics of Genjaegotaek Galabjib (건재고택 가랍집의 건축적 특성에 관한 연구)

  • Kim, Suk-Hee
    • Journal of the Korea Convergence Society
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    • v.9 no.11
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    • pp.263-269
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    • 2018
  • Asan Oeam village is a town that has been formed since about 500 years ago. It is a folk village centered on Yean Lee and designated as National Folk Cultural Property No. 236. However, the original shape of the thatch, which has regional characteristics, is disappearing. Therefore, I want to study the architectural characteristics of the house which is well preserved in the original shape. The Genjaegotaek is located in the center of the Oeam village. The composition of the house consists of a Munganchae, Salangchae, Anchae, Sadang. The Anchae is an 'ㄱ' shaped house, and it is shaped like a 'ㅁ' which is opened by facing. The wide yard in front of the Salangchae is made of strangely shaped rocks, a hundred-year-old pine, old pagodas, ponds, pavilion. Outside the main gate, there is an 'ㅡ' shape thatch on the west. The Galabjib of Geonjaegotaek is a servant house, and the shape is a ㄱ type. The plane remained well in its original shape, thatch is consisting of a kitchen-room-daecheong maru-room. A Galabjib of Geonjaegotaek can not represent all the thatch of a Oeam village. However, it is expected that this study will be used as a basic data when the study by the thatch in this area is conducted through the analysis of the type.

Non-face-to-face online home training application study using deep learning-based image processing technique and standard exercise program (딥러닝 기반 영상처리 기법 및 표준 운동 프로그램을 활용한 비대면 온라인 홈트레이닝 어플리케이션 연구)

  • Shin, Youn-ji;Lee, Hyun-ju;Kim, Jun-hee;Kwon, Da-young;Lee, Seon-ae;Choo, Yun-jin;Park, Ji-hye;Jung, Ja-hyun;Lee, Hyoung-suk;Kim, Joon-ho
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.3
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    • pp.577-582
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    • 2021
  • Recently, with the development of AR, VR, and smart device technologies, the demand for services based on non-face-to-face environments is also increasing in the fitness industry. The non-face-to-face online home training service has the advantage of not being limited by time and place compared to the existing offline service. However, there are disadvantages including the absence of exercise equipment, difficulty in measuring the amount of exercise and chekcing whether the user maintains an accurate exercise posture or not. In this study, we develop a standard exercise program that can compensate for these shortcomings and propose a new non-face-to-face home training application by using a deep learning-based body posture estimation image processing algorithm. This application allows the user to directly watch and follow the trainer of the standard exercise program video, correct the user's own posture, and perform an accurate exercise. Furthermore, if the results of this study are customized according to their purpose, it will be possible to apply them to performances, films, club activities, and conferences

Regional Topographic Characteristics of Sand Ridge in Korean Coastal Waters on the Analysis of Multibeam Echo Sounder Data (다중빔음향측심 자료분석에 의한 한국 연안 사퇴의 해역별 지형 특성)

  • BAEK, SEUNG-GYUN;SEO, YOUNG-KYO;JUNG, JA-HUN;LEE, YOUNG-YUN;LEE, EUN-IL;BYUN, DO-SEONG;LEE, HWA-YOUNG
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.27 no.1
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    • pp.33-47
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    • 2022
  • In this study, distribution of submarine sand ridges in the coastal waters of Korea was surveyed using multibeam echo sounder data, and the topographic characteristics of each region were identified. For this purpose, the DEM (Digital Elevation Model) data was generated using depth data obtained from the Yellow Sea and the South Sea by Korea Hydrographic and Oceanographic Agency, and then applied the TPI (Topographic Position Index) technique to precisely extract the boundary of the sand ridges. As a result, a total of 200 sand ridges distributed in the coastal waters were identified, and the characteristics of each region of the sedimentary sediments were analyzed by performing statistical analysis on the scale (width, length, perimeter, area, height) and shape (width/length ratio, height/width ratio, linear·branch type, exposure·non-exposure type). The results of this study are expected to be used not only for coastal navigational safety, but also for marine naming support, marine aggregate resource identification, and fisheries resource management.

A Study on the Influence of Parenting Attitude of a Multicultural Family Mother on the School Readiness and Sensory Processing Skill (다문화가정 어머니의 양육태도가 아동의 학교준비도와 감각처리능력에 미치는 영향)

  • Song, Kyeong-Ah;Oh, Myung-Hwa;Kim, Hye-Mi;Kim, Jeong-Ja
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.8
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    • pp.531-539
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    • 2019
  • This study was intended to investigate the effects of parenting attitude of mothers of multicultural family and sensory processing skills of children on school readiness of preschool children. This study conducted Maternal Behavior Instrument(MBRI), Short Sensory Profile(SSP), and School readiness test on children of multicultural family and their mothers who are residing in Gwangju and Jeonnam, and results are as follows. According to the result of parenting attitude of mothers of multicultural family showed that total score 165.72±24.19. According to the result of investigating a correlation between parenting attitude of mothers of multicultural family and sensory processing skills of children on school readiness, only parenting attitude of mothers of multicultural family showed positive relationship with school readiness of children(p<.05). According to the result of investigating the effects of parenting attitude of mothers of multicultural family and sensory processing skills of children on school readiness, it was identified that parenting attitude of mothers of multicultural family makes significant effect on school readiness of children. To sum up, it is considered that parents education required for developing positive parenting attitude of mothers of multicultural family and mediations related to school readiness for school life adaptation of children of multicultural family are necessary for children of multicultural family to get adjusted to school life easily after they enter school.

Microscopic Anatomy of Male Reproductive Organ in the Long Arm Octopus Octopus minor (Cephalopoda: Octopodidae) (낙지 Octopus minor 수컷 생식기관의 미세해부학적 구조)

  • Seong Jin Kim;Hyeon Jin Kim;So Ryung Shin;Myeong Gyo Seo;Pyeong Woo Kim;Eun Ha Kim;Jung Sick Lee
    • Journal of Marine Life Science
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    • v.8 no.2
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    • pp.178-185
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    • 2023
  • This study was described the microscopic anatomy of male reproductive organs and spermatophore necessary for understanding the reproductive ecology of the long arm octopus Octopus minor. The long arm octopus was a species that has sexual dimorphism that can distinguish between sex based on the presence of hectocotylus. Male reproductive organs consisted of testis, primary spermatic duct, spermatic gland, secondary spermatic duct, spermatophoric gland and spermatophoric sac. Histologically, the testis was testicular tubule type and male germ cells showed a layered arrangement. The primary spermatic duct was a tube connecting the testis and spermatic gland, and consisted with epithelial layer and connective tissue. The spermatic gland was located between the primary and secondary spermatic duct, and the epithelial layer was composed of epithelial cells and mucous cells. Mucous cells reacted blue in the AB-PAS (pH 2.5) reaction and purple in the AF-AB (pH 2.5) reaction. The secondary spermatic duct was a short tube connecting spermatic gland and spermatophoric gland, and folds were developed in lumen. The spermatophoric gland consisted of numerous tubular glands and secretory cells had eosinophilic granules. The spermatophoric sac was shape of pouch, folds were developed in lumen, and vacuolar secretory cells were present in the epithelial layer. The spermatophore was 83.5 mm long and consisted of cap thread in anterior portion, ejaculatory apparatus and cement body in medial portion, sperm mass in posterior portion.

Diagnosis of Real Condition and Distribution of Protected Trees in Changwon-si, Korea (창원시 보호수의 분포현황과 실태진단)

  • You, Ju-Han;Park, Kyung-Hun;Lee, Young-Han
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.29 no.1
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    • pp.59-70
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    • 2011
  • The purpose of this study is to present raw data to systematically and rationally manage the protected trees located in Changwon-si, Korea. This study investigated about the present condition and the information of location, individual, management, health and soil. The results are as follows. The protected trees were located in 26 spots, and species of trees were 9 taxa; Zelkova serrata, Celtis sinensis, Aphananthe aspera, Ginkgo biloba, Carpinus tschonoskii, Pinus densiflora for. multicaulis, Quercus variabilis, Pinus densiflora and Salix glandulosa. In protected tree types, shade trees were the most, and the majority of theirs were 200 years or more in age. The range of altitude was 14~173m, and the number of trees located in flat fields was the most. For location types, village and field and mountain were presented in the order and, in land use, land for building was the most. The range of height was 8.0~30.0m, 0.6~5.1m in crown height, 240~700cm in diameter of breast and 210~800cm in diameter of root. In case of crown area, Zelkova serrata of No.5 was most large. The status boards were mostly installed except No.23 and No.26. The sites with fence were 9 spots, and the site with stonework were 14 spots. The sites with the support beam were 5 spots, and most sites were not covered up with soil. The materials of bottom were soil, gravel and vegetation in the order. The range of withering branch rate was 0~40%, and peeled bark rate was 0~60%. The sites made holes were 23 spots, and the hole size of Aphananthe aspera of No.12 was the largest. The sites disturbed by human trampling were 7 spots, the sites by disease and insects of 2 spots, the sites by injury of 23 spots and the sites by exposed roots of 13 spots. In the results of soil analysis, there showed that acidity was pH 4.5~8.0, organic matter content of 3.5~69.8g/kg, electrical conductivity(EC) of 0.11~2.87dS/m, available $P_2O_5$ of 3.0~490.6mg/kg, exchangeable K of 0.10~1.05cmol+/kg, exchangeable Ca of 1.41~16.45cmol+/kg, exchangeable Mg of 0.37~1.96cmol+/kg, exchangeable Na of 0.25~2.41cmol+/kg and cation exchange capacity(C.E.C) of 8.35~26.55cmol+/kg.