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Optimization of Submerged Culture Conditions for Protease Production and Its Enzymatic Properties (Protease 생산을 위한 최적 배양조건 및 생산된 Protease의 특성)

  • Cho, Hee-Yeon;Cho, Nam-Seok
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.5
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    • pp.12-19
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    • 2004
  • This study was performed to investigate the optimum condition of protease production from submerged culture of oak mushroom (Lentinula edodes, Sanlim No. 5) and its enzymatic features. Among several combinations of media, the combination of wheat bran, corn flour, water and corn oil (WB+CF+W+ CO) yielded 84.8 U/g of maximum protease activity. This combination of ingredients, in spite of not being particularly protein-rich in comparison to the other media, allowed for good growth of the fungus and maximal protease production. Comparison of different growth medium liquids indicated that demineralized water afforded the best growth of the fungus and the highest protease activity. Acetate buffer and acidified water negatively affected The protease production peaked around 72 hr of incubation, and decreased thereafter. The molecular weights of produced protease were about 45,000 by Sephadex G-75 chromatography. The pH optimum for protease activity was 4, while maximal activity incubated at 37℃ for 1 hr was observed between pH 4~6. The optimum temperature of this protease was 55℃, and the enzyme was active over a broad temperature range (30~60℃), indicating that this protease would be suitable for a wide range of applications where. different pH and temperature are necessary, such as digestive aids, food industry, beer and tannery industries.

A Study on Development and Effectiveness of the Indicatives for Analysis of the Effects of a Book Sharing Project on pre-schoolers of Supporter' Reading Care in Gyeonggi-do (경기도 책꾸러미 사업을 통한 양육자의 독서육아 효과 분석을 위한 지표개발 및 효과성 연구)

  • Choi, In-Ja;Yoon, Sung-Une;Kim, Soo-Kyoung;Hoang, Gum-Sook;Lee, Sun-Ai
    • Journal of the Korean Society for Library and Information Science
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    • v.56 no.2
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    • pp.133-155
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    • 2022
  • The purpose of this study was to develop the indicatives for the analysis of the effects of Gyeonggi-do Book Sharing Project on pre-schoolers of supporter' reading care and thereby, suggest some data useful to the establishment of a reading culture promotion policy in Gyeonggi-do. Preceding studies and cases were reviewed to analyze the effects of the book-sharing project on pre-schoolers of supporter' reading care and thereby, develop some measurement indicatives, and thus, the indicatives were verified by professionals using the Delphi technique. Then, supporter of 3~5 year-old pre-schoolers were sampled from 7 cities and counties in Gyeonggi-do (Pocheon-si, Yangpyeong-gun, Yeoju-si, Dongducheon-si, Gapyeong-gun, Yeoncheon-gun and Yangju-si) to be divided into control and test groups and thereby, their reading care effect indicatives were compared before and after the test. The theoretical background is theory of family literacy, emergent literacy and parenting efficacy. As a result of developing the indicatives for analysis of pre-schoolers of supporter's reading care effects and comparing them for the sample pre-schoolers of supporter, before and after the test, the book-sharing project was found effective in improving reading care. The most difficult problem in pre-schoolers' earlier reading education involves acquisition of reading habit. So, it is deemed necessary to operate a regular book sharing project involving public organization and homes. As a result of developing the indicatives and analyzing the effects of the book-sharing project, it was confirmed that the project would serve to improve pre-schoolers of support's reading care and therefore, this study seems to provide some ground for the operation of a sustainable book-sharing project to narrow the education divide and promote a book reading culture in Gyeonggi-do.

The Effect of Case-Based Health Assessment Practical Education on Class Participation, Problem Solving Process, Academic Self-Efficacy and Academic Achievement of Nursing Students (간호대학생의 사례기반 건강사정 실습교육 프로그램이 문제해결과정, 수업참여도, 학업적 자기효능감, 학업성취도에 미치는 효과)

  • Cho, Young-Mun
    • Journal of Digital Convergence
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    • v.20 no.2
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    • pp.499-509
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    • 2022
  • This study was conducted to understand the effect of health assessment practical education on class participation, problem-solving process, academic self-efficacy, and academic achievement of nursing students. This study used a nonequivalent control group pretest-posttest design. The participants were 69 nursing university students located in C city. Data were collected on two separate occasions before and after the application of the program from February 2021 to July 2021. Data were analyzed by chi-square test, independent t-test, and ANCOVA using SPSS WIN 23.0. There were significant differences in class participation(F=15.003, p<.001), academic self-efficacy(F=13.288, p=.001) and academic achievement(F=19.755, p<.001) between the experimental group and the control group. In the problem-solving process, the experimental group was significantly higher than the control group in decision-making(F=6.948, p=.010), applying the solution(F=6.232, p=.015) and evaluation-reflection(F=5.364, p=.024). It is necessary to expand case-based learning to increase the problem-solving process, class participation, academic self-efficacy, and academic achievement of nursing students.

Alternatives to Improving the Curriculum of Teacher Training Institutions to Enhance Future Responsiveness (미래 대응력 제고를 위한 교원양성기관의 교육과정 개선 방안)

  • Shin, Min-Hye;Kim, Seung-Yong
    • Journal of Digital Convergence
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    • v.20 no.2
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    • pp.447-454
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    • 2022
  • The purpose of this study was to strengthen the practicality of preliminary teachers in preparation for future education, to respond to structural changes in the teacher training system due to a decrease in the school-age population, and to seek a future-oriented direction for the curriculum of teacher training institutions. To this end, we analyzed the competency diagnosis items of the teacher training institutes in the 5th cycle from 2019 to 2020, and based on the proposal for the development plan for the teacher training system announced by the Ministry of Education in July 2021 and the contents of the 4 discussions, content was presented. The results were as follows. First, to nurture creative and convergence-type talents, teacher training institutions need to develop multi-curricular competency and reorganize the curriculum into a future-oriented curriculum. Second, in order to foster the competence of teachers and preliminary teachers in teacher training institutions, it is essential to strengthen the competence of teachers through the introduction of the metaverse, general teaching methods, and ICT-using teaching methods. Third, teachers' training institutions will introduce and strengthen the 'education practice teacher homeroom system' to strengthen school field-oriented practical competencies, 'dedicated mentor-mentee' centered on seniors and juniors, 'monitoring system' led by university professors, and 'probationary teacher system'

Publication Dating through Observations of Differences in Woodblock Printing Characteristics among Various Versions of Wanpanbon Editions of Honggildongjeon (Tale of Hong Gil Dong) (완판본(完板本) 홍길동전 판본 간에 나타난 목판인쇄 특징의 차이점 관찰을 통한 간행 연대의 추정)

  • Yoo, Choon Dong;Yoo, Woo Sik
    • Journal of Conservation Science
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    • v.38 no.2
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    • pp.96-108
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    • 2022
  • To understand the process of publication and distribution of ancient Korean novels under the Joseon dynasty and Japanese occupation, collecting information on the publication of various printed materials and estimating their publication date through various methods is extremely important. In this study, the characteristics and differences that appeared in the woodblock printing process were investigated using image comparison among editions of the Honggildongjeon (Tale of Honggildong) of Wanpanbon (完板本) (published in the Jeonju region) with other commercial editions of the ancient Korean novels. Additionally, the publication year of each edition was estimated. Printed images of different versions were compared with those of a recently discovered original series version and the changes in the process of carving replacement woodblocks were investigated and summarized. Various phenomena, such as differences in fonts, borderline shapes and integrity of printed characters, appearing in woodblock prints provided useful information for determining chronological relationships between the prints and estimating the approximate publication year for each edition. The various characteristics of woodblock printing obtained through this study are expected to serve as a reference for estimating the relationship between the printing method and the approximate publication date of old books and paper-based printed historical records.

Estimation of Employment Creation Center considering Spatial Autocorrelation: A Case of Changwon City (공간자기상관을 고려한 고용창출중심지 추정: 창원시 사례를 중심으로)

  • JEONG, Ha-Yeong;LEE, Tai-Hun;HWANG, In-Sik
    • Journal of the Korean Association of Geographic Information Studies
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    • v.25 no.1
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    • pp.77-100
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    • 2022
  • In the era of low growth, many provincial cities are experiencing population decline and aging. Population decline phenomena such as reduction of productive manpower, reduction of finances, deterioration of quality of life, and collapse of the community base are occurring in a chain and are being pushed to the brink of extinction of the cities. This study aims to propose a methodology to objectively estimate the employment creation centers and setting the basic unit of industrial-centered zoning by applying spatial statistical techniques and GIS for the application of the compact city plan as an efficient spatial management policy in a city with a declining population. In details, based on reviewing previous studies on compact city, 'employment complex index(ECI)' were defined considering the number of workers, the number of settlers, and the area of development land, the employment creation center was estimated by applying the 'Local Moran's I' and 'Getis-Ord's Hot-Spot Analysis'. As a case study, changes in the four years of 2013, 2015, 2017, and 2019 were compared and analyzed for Changwon City. As a result, it was confirmed that the employment creation center is becoming compacted and polycentric, which is a significant result that reflects the actual situation well. This results provide the basic data for functional and institutional territorial governance for the regional revitalization platform, and provide meaningful information necessary for spatial policy decision-making, such as population reduction, regional gross domestic product, and public facility arrangement that can respond to energy savings, transportation plans, and medical and health plans.

An Empirical Study on the Effects of the Values of Socialism to the Entrepreneurial Intentions of North Korean Refugees (북한이탈주민의 가치체계가 창업의도에 미치는 영향에 관한 연구)

  • Noh, Kyu-Duk;Lee, Jung-Hee;Lee, Il-Han
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.16 no.6
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    • pp.107-117
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    • 2021
  • The purpose of this study is to examine the causal relationship of related variables in order to promote entrepreneurship, which is one way to support the economic independence and stable settlement of North Korean defectors. This study is firstly to examine the influence of individualism and collectivism on entrepreneurial intentions, and secondly, to examine the mediating effect of social adaptability in the relationship between socialist value system and entrepreneurial intention. To this end, a survey was conducted on North Korean defectors, and the empirical analysis target was 223 North Korean defectors. For the verification of the causal relationship between personal disposition and entrepreneurial intention, and the causal relationship between value system and entrepreneurial intention PLS was used. And PROCESS for SPSS Macro was used to verify the mediating effect of social adaptation in the relationship between the value system and entrepreneurial intention. Looking at the results of the empirical analysis, the extraversion factor was found to have a positive effect on the entrepreneurial intention level of North Korean defectors, and the self-fulfillment desire factor was found to have a positive effect on the entrepreneurial intention. It was also found that individualist values have a positive effect on the entrepreneurial intentions of North Korean defectors, and collectivist values do not have a significant effect on the entrepreneurial intentions. As a result of examining the mediating effect of social adaptability between individualism/collectivism and entrepreneurial intention in this study, it was found that individualist value has a direct impact on entrepreneurial intention without engaging social adaptability.

A Study on the National Library of Korea Librarians' Perceptions of Change Management (변화관리에 대한 국립중앙도서관 사서의 인식에 관한 연구)

  • Dong-Jo, Noh
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.33 no.4
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    • pp.49-72
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    • 2022
  • This study based on the 8-step strategy model for change management proposed by J. P. Kotter, tried to check the perception and experience about change management of the librarians at National Library of Korea. Also this study aimed to figure out the understanding of each 8-step strategy model. To this end, the concept and process of change management were presented through literature analysis, and survey was conducted targeting 58 librarians of the National Library of Korea. This study drews five results. First, the majority of librarians at National Library of Korea do not know about change management, and they do not have experience in using change management techniques. Second, the period required for the 8-step change management process is 5 years. Third, during the change management stage, librarians' awareness of the importance of stages 4 and 3 was high. Fourth, there is no difference in the librarians' perceptions of change management by gender, education, major, and tasks. Fifth, there is a significant difference in the age and service years of the librarian in the 3rd, 4th, and 7th stages of change management. Also there is a significant difference in the position of the librarian at the 1st and 3rd stage of change management, and between librarians' department and 1st stage of change management. The findings and results of this study can not only raise awareness of change management but also suggest organizational responses to change at individual or organizational level.

Effects of Practical Training Using 3D Printed Structure-Based Blind Boxes on Multi-Dimensional Radiographic Image Interpretation Ability (3D 프린팅 구조물 기반 블라인드박스를 이용한 실습교육이 다차원 방사선영상해독력에 미치는 효과)

  • Youl-Hun, Seoung
    • Journal of the Korean Society of Radiology
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    • v.17 no.1
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    • pp.131-139
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    • 2023
  • In this study, we are purposed to find the educational effect of practical training using a 3D printed structure-based blind box on multidimensional radiographic image interpretation. The subjects were 83 (male: 49, female: 34) 2nd year radiological science students who participated in the digital medical imaging practice that was conducted for 3 years from 2020 to 2022. The learning method used 3D printing technology to print out the inside structure of the blind box designed by itself. After taking X-rays 3 times (x, y, z axis), the structure images in the blind box were analyzed for each small group. We made the 3D structure that was self-made with clay based on our 2D radiographic images. After taking X-rays of the 3D structure, it was compared whether it matches the structural image of the blind box. The educational effect for the practical training surveyed class faithfulness, radiographic image interpretation ability (attenuation concept, contrast concept, windowing concept, 3-dimensional reading ability), class satisfaction (interest, external recommendation, immersion) on a 5-point Likert scale as an anonymous student self-writing method. As a result, all evaluation items had high positive effects without significant differences between males and females. Practical education using blind boxes is a meaningful example of radiology education technology using 3D printing technology, and it is expected to be used as content to improve students' problem-solving skills and increase satisfaction with major subjects.

Analysis on the Changes in Abandoned Paddy Wetlands as a Carbon Absorption Sources and Topographic Hydrological Environment (탄소흡수원으로서의 묵논습지 변화와 지형수문 환경 분석)

  • Miok, Park;Sungwon, Hong;Bonhak, Koo
    • Land and Housing Review
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    • v.14 no.1
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    • pp.83-97
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    • 2023
  • The study aims to provide an academic basis for the preservation and restoration of abandoned paddy wetland and the enhancement of its carbon accumulation function. First, the temporal change of the wetlands was analysed, and a typological classification system for wetlands was attempted with the goal of carbon reduction. The types of wetland were classified based on three variables: hydrological environment, vegetation, and carbon accumulation, with a special attention on the function of carbon accumulation. The types of abandoned paddy wetlands were classified into 12 categories based on hydrologic variables- either high or low levels of water inflow potential-, vegetation variables with either dominance of aquatic plants or terrestrial plants, and three carbon accumulation variables including organic matter production, soil organic carbon accumulation, and decomposition. It was found that the development period of abandoned paddy analyzed with aerial photographs provided by the National Geographic Information Institute happened between 2010 and 2015. In the case of the wetland in Daejeon 1 (DJMN01) farming stopped by 1990 and it appeared to be a similar structure to natural wetlands after 2010 . Over the past 40 years the abandoned paddy wetland changed to a high proportion of forests and agricultural lands. As time went by, such forests and agricultural lands tended to decrease rapidly and the lands were covered by artificial grass and other types of forests.