• Title/Summary/Keyword: 자방

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Plant Regeneration from Unpollinated Ovary Culture in Allium tuberosum Rottl (부추의 미수분 자방배양에 의한 식물체 재분화)

  • 윤수진;손재근;권용삼
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.1
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    • pp.37-40
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    • 1999
  • This study was carried out to determine the optimal conditions for the production of plants derived from the unpollinated ovary culture of Chinese chive (Allium tuberosum Rottl.). The Chinese chive collected from Korea showed much higher frequency of plantlet formation than those from Japan in the culture of unpollinated ovaries. Among the collections, 'Youngiljaerae' showed the highest frequency of plantlet formation. The MS basal medium was superior to B/sub 5/ in plantlet formation. The ovaries inoculated on the 2,4-D-free medium were directly induced plantlets without callus formation. Floral parts inoculated as a unit played important roles in callus formation and plant production. The frequency of callus and plantlet formation was higher in the culture of ovary with anthers than that of ovary alone.

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Factors Affecting plant Regeneration in Unpollinated Ovary Culture of Rice (벼의 미수분 자방배양에 영향을 미치는 요인)

  • 손재근;권용삼;김경민
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.6
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    • pp.319-322
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    • 1997
  • The optimal conditions for callus formation and plant regeneration were determined by the manipulation of culture method in unpollinated ovary culture of rice. The effect of cold pretreatment on callus formation and plant regeneration varied with duration of pretreatment. The maximum frequency (38.7%) of plant regeneration was obtained from the unpollinated ovary pretreated for 10 days at $12^{\circ}C$. The ability of callus formation and plant regeneration was higher on the medium with picloram (1 mg/L) than that of 2, 4-D (1 mg/L). However, the high concentration of picloram increased markedly the frequency of albino plant from unpollinated ovary-derived callus. Floral parts inoculated as a unit play important roles in callus formation and plant regeneration. Best result was obtained when ovary with partial cut glume, pedicel and secondary branch as a unit was cultured.

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Effect of deposition temperature on field emission property of carbon thin film grown by PECVD (PECVD에 의해 작성된 탄소계 박막의 전계전자방출특성에 대한 증착온도 의존성에 관한 연구)

  • ;;M. Katayama;;K. Oura
    • Journal of the Korean Vacuum Society
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    • v.12 no.1
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    • pp.35-39
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    • 2003
  • Using RF plasma enhanced chemical vapor deposition, amorphous carbon films were grown in pure methane plasma. Field electron emission of these films were examined at a function of deposition temperature. It was found that the electron emission current of the sample prepared at deposition temperature above $600^{\circ}C$ was considerably improved. The film grown at deposition temperature of $800^{\circ}C$ had the best threshold field of 8 V/$\mu\textrm{m}$ in this experiment. According to the results of Raman spectroscopy. growth of graphite crystallites was promoted with higher deposition temperatures. Moreover the surface morphology was abruptly changed at deposition temperature above $600^{\circ}C$. We discuss the field electron emission characteristics of amorphous carbon films with regard to the structural feature and surface morphology.

In-vitro Anti-thrombosis Activities of Different Parts of Nelumbo nucifera Gaertner (다양한 부위별 연 추출물의 항혈전 활성)

  • Ahn, Seon-Mi;Sung, Hwa-Jung;Kim, Jong-Sik;Park, Jong-Yi;Sohn, Ho-Yong
    • Microbiology and Biotechnology Letters
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    • v.46 no.4
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    • pp.372-376
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    • 2018
  • To investigate the bio-activities of lotus (Nelumbo nucifera Gaertner), ethanol extracts were prepared from different parts of lotus, including lotus root, node of root (NR), leaf, pod of seed (PS), seed (S), and embryo of seed (ES), respectively. Anti-thrombosis activities were evaluated. NR, S, and PS extracts displayed strong anti-coagulation activities, as evidenced by the inhibition of thrombin, prothrombin, and coagulation factors, respectively. NR, leaf, S, and PS extracts displayed strong platelet aggregation inhibitory activities. The aggregation inhibition of S and PS extract was comparable to that of aspirin. The extracts of NR, S, and PS did not show hemolysis activity up to 1.0 mg/ml.

Effect of Seed Parents on Varietal Performance in Strawberry (딸기 교잡육종 시 품종적 성능에 미치는 자방친의 효과)

  • Rho, Il Rae;Cho, Yong Seop;Cheong, Jae Wan;Jeong, Ho Jeong
    • Korean Journal of Breeding Science
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    • v.42 no.4
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    • pp.374-380
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    • 2010
  • In order to select excellent cross parents in strawberry breeding, 11 varieties were self-pollinated and tested their progenies. Among the 11 varieties, 'Akihime', 'Maehyang' and 'Seolhyang' showed superior in plant vigor, disease resistance and yield. Combining ability was tested using 15 progenies obtained from crosses between 3 selected varieties as seed parents and 5 varieties as pollen parents. Plant vigor was the strongest when 'Akihime' was used as a seed parent while ripening time tended to the fastest when 'Seolhyang' as a seed parent. Average fruit weight of the crosses 'Seolhyang' ${\times}$ 'Tochiotome', 'Seolhyang' ${\times}$ 'Geumhyang', 'Seolhyang' ${\times}$ 'Wongyo 3111' and 'Akihime' ${\times}$ 'Wongyo 3111' were above 18 g for marketable fruits. Fruit hardness was superior at crosses of 'Maehyang' as a seed parents to other crosses combinations, especially 'Maehyang' ${\times}$ 'Tochiotome'. Soluble solid contents (SSC) was not significantly different among cross combinations. The best cross combination for marketable yield was 'Akihime' ${\times}$ 'Wongyo 3111', followed by 'Seolhyang' ${\times}$ 'Geumhyang', 'Maehyang' ${\times}$ 'Geumhyang', 'Akihime' ${\times}$ 'Tochiotome', in order.

Quality Control on Neutron Activation Analysis for Urban Dust by the Proficiency Test (비교숙련도 시험을 통한 도시대기분진에 대한 중성자방사화분석법의 품질관리)

  • Moon, Jong-Hwa;Kim, Sun-Ha;Chung, Yong-Sam
    • Analytical Science and Technology
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    • v.15 no.5
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    • pp.433-438
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    • 2002
  • Trace elements in two air filters (V-50, P-50) artificially loaded with urban dust provided from IAEA were determined non-destructively using instrumental neutron activation analysis. Standard reference material (Urban Particulate Matter, SRM 1648) of National Institute of Standard and Technology was used for analytical quality control. About 20 elements in both of loaded filter samples were determined. To evaluate inter-laboratory comparison and proficiency test, analytical data were statistically treated with the results which collected from 49 laboratories, 40 countries participated in this study using neutron activation analysis, particle induced X-ray emission, inductively coupled plasma mass spectroscopy, etc,. From the results of statistical treatment, Z-scores are within ${\pm}2$. Furthermore, accuracy and precision of obtained analytical values are passed according to the criteria of the proficiency test. Consequently, it was proved that analytical quality for air dust samples being performed has been controlled properly.

Effect of Low Temperature and Hot-Water Treatment on Germinatino of Seeds in Dendropanax morbifera $L_{EV}$. (저온 및 온탕 침지가 황칠나무 종자의 발아에 미치는 영향)

  • 최성규
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.101-105
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    • 1998
  • This study was carried out to find out the method of increasing seed germination rate of Dendropanax morbifera LEV. was drupaceous fruit. The length and width of ovarywas 7.8 to 10.7mm, 6.9 to 9.0mm, respectively, and the shape was elliptical. The ovary had 5 loculi with one speed per loculus , but one seed among 5 weeds was exalbuminous seed. The length and width of seed was 6.3 to 7.4mm, 2.0 to2.mm, respectively, and 1,000 seed weight was 14.3 to 18.0g. Seeding after stroring at $10^{\circ}C$ for 60 to 90 days was germinated to 87% . However, fall seeding directly after harvest was increased to 85% at hot-water treatement ($40^{\circ}C$) for 90 to 120 minute.

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Effects of Days after Pollination and Media on Asymbiotic Germination in Cross Combination of Cymbidium spp. (수분 후 경과일수 및 배지가 심비디움 교배조합의 무균발아에 미치는 영향)

  • Kim, Mi Seon;Lee, Young Ran;Won, Je Yang;Kim, Jae Yeong;Kim, Byeong Hyeon;Eun, Jong Seon
    • Horticultural Science & Technology
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    • v.16 no.2
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    • pp.236-238
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    • 1998
  • This study was conducted to investigate the effective media and optimal days after pollination for germination of seeds which were crossed by selfing or reciprocal pollination in Cymbidium spp. The elongation of ovary stopped about 110 days after pollination regardless of cross combinations. The increase of ovary width stopped about 140 days after pollination showing some differences among cross combinations. The cross combinations expressing high capsule number and viability showed good germination when they were sown in vitro. The capsules collected 150 to 180 days after pollination showed good germination performance and short days of germination. Hyponex media was the most effective for protocorm development and germination.

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Taxonomic Studies of Korean Tripterospermum japonicum (Max.) Max. and Pterygocalyx volubilis Max. (한국산 덩굴용담과 좁은잎덩굴용담의 분류학적 재검토)

  • 백원기
    • Korean Journal of Plant Resources
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    • v.16 no.1
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    • pp.61-73
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    • 2003
  • External and anatomical characters such as stem, leaf, ovary, calyx lobe, ultrastructure of stigma epidermis of leaf blade and midvein, corolla lobe, seed coat and pollen were examined on Pterygocalyx volubilis and Tripterospermum japonicum of Korean Gentianaceae. Fruit characters(capsule and berry), absent or not of corona and pollen characters(reticulate and striate) has been used by identification characters of two taxa. But study results, external characters(root, leaf, calyx lobe, stigma, anther), internal characters(calyx love, ovary) and ultrastructure characters(epidermis o leaf blade and midvein, corolla lobe, seed coat) were useful for species identification. Taxonomic relationships among problematic taxa were discussed.