• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.446-453
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• 2009

RAPD analyses were performed to investigate genetic relationships and useful molecular maker for 3 species and their 17 regional populations of the Pueraria lobata and related taxa. The length of amplified DNA fragments ranged from 200 to, 2,800 bp. Two hundred and eight scorable polymorphic makers and three scorable monomorphic makers were found from the PCR reactions with 15 random oligo primers, and those were analyzed by Nei's genetic distance coefficient. Based on the UPGMA phenogram from RAPD analyses, two major groups (9 populations from Korea; 3 populations from foreign countries) were recognized. And it showed distinct genetic differences from related taxa. The RAPD results was very useful to define the samples by geographical distribution and to discuss the relationships among the populations and their related taxa of the Pueraria lobata.

• The Journal of the Korea institute of electronic communication sciences
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• v.10 no.12
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• pp.1321-1328
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• 2015

A radio is a half-duplex voice communication method using the PTT(: Push To Talk), occupy a single line calls during transmission. As an interface between the telephone and the radio, UHF and VHF, for voice communication between the different heterogeneous devices, A device automatically switches between the two devices is required. Therefore, in accordance with the performance of the voice switching apparatus for detecting a voice to be transmitted from an input signal, loss of the audio signal to be transmitted is subjected to Significant influence. Conventional method has the problem responding to noise by setting the level through simple means of amplitude of input signal, in other words, the energy level of the input signal. This paper, by using the audio signal processing techniques, this discriminated what the voice is among the input signal and substantiated a device for the automatic voice transmission between heterogeneous devices. With this proposal, I was confirmed of improvement of performance in the automatic voice switching device, could perform loss-less transmission of voice between heterogeneous devices.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.2
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• pp.449-455
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• 2004

This paper presents a novel defect detection method for one chip RF front end with fault detection circuits using input matching measurement. We present a BIST circuit using 40.25{\mu}m$ CMOS technology. We monitor the input transient voltage of the RF front end to differentiate faulty and fault-free RF front end. Catastrophic as well as parametric variation fault models are used to simulate the faulty response of the RF front end. This technique has several advantages with respect to the standard approach based on current test stimulus and frequency domain measurement. Because DUT and fault detection circuits are implemented in the same chip, this test technique only requires use of digital voltmeter (RMS meter) and RF voltage source generator for simpleand inexpensive testing.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.10a
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• pp.823-824
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• 2016

In general, distribution board, panel board and motor control center can be installed over a wide area such as residence of group, building, schools, factories, ports, airports, water service and sewerage, substation and heavy industries that are used to supply converts the voltages extra high voltage into optimal voltage. There are electrical accidents due to rise of contact temperature, loose contact of bus bar, deterioration of the contact resistance, overtemperatue of the bus bars. In this paper, we propose bus bar joints monitoring system with loose connection of bus bar, measuring the joint resistance of busbars and monitoring internal and external heat. The proposed system can be reduced the electrical accidents by maintenance of busbar joints and the temperature of the conductive contact surface of busbars.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.867-871
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• 2022

Amid the recent rapid trend change, the change in design has a great impact on the sales of fashion companies, so it is inevitable to be careful in choosing new designs. With the recent development of the artificial intelligence field, various machine learning is being used a lot in the fashion market to increase consumers' preferences. To contribute to increasing reliability in the development of new products by quantifying abstract concepts such as preferences, we generate new images that do not exist through three adversarial generative neural networks (GANs) and numerically compare abstract concepts of preferences using pre-trained convolution neural networks (CNNs). Deep convolutional generative adversarial networks (DCGAN), Progressive growing adversarial networks (PGGAN), and Dual Discriminator generative adversarial networks (DANs), which were trained to produce comparative, high-level, and high-level images. The degree of similarity measured was considered as a preference, and the experimental results showed that D2GAN showed a relatively high similarity compared to DCGAN and PGGAN.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.2
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• pp.231-240
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• 2009

A total of 26 Korean elite soybean cultivars including 21 certified cultivars was assessed to evaluate genetic diversity and to analyze relationship among them based on 15 SSR markers. Fifteen SSR markers generated a total of 201 alleles. Average number of alleles per SSR marker was 13.4 with a range from 8 to 19. Genetic diversity of 26 cultivars estimated by PIC value ranged from 0.782 to 0.931 with an average of 0.874. PIC value of Satt197 was the highest with 0.931 and Satt141 was the lowest with 0.782 among 15 SSR markers. Cluster analysis based on genetic distances classified 26 soybean cultivars into 3 clusters. Cluster I, II and III included 2, 7 and 17 cultivars, respectively. Average genetic diversity within clusters was 0.769 with a range from 0.720 to 0.799. Average genetic diversity between clusters was 0.813 with a range from 0.725 to 0.857. Genetic diversity was higher between clusters than within clusters. Genetic relationship among clusters was near between I and II, and I and III and far between II and III cluster. All of 26 Korean elite soybean cultivars could be identified by using any of 5 combinations of 2 SSR markers with higher PIC value, i.e, $Satt197+Sat_088$, Satt197+Satt245, $Sat_088+Sat_-036$, $Sat_088+Satt245$ and Satt185+Satt245.

• Journal of Food Hygiene and Safety
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• v.37 no.2
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• pp.46-54
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• 2022

Known for its effectiveness in weight loss and diabetes prevention, Gymnema sylvestre products can be found in the US, Japanese, and Indian markets. However, the recommended dosage or safety of these products has not yet been proven. Therefore, development of an analytical method for detecting the content of Gymnema sylvestre in food products is required. Accordingly, this study proposes an analysis method that can examine Gymnema sylvestre in food using LC-ESI-MS/MS and KASP (Kompetitive Allele-Specific PCR) markers. In LC-ESI-MS/MS, a simultaneous analysis method for gymnemic acid and deacylgymnemic acid was optimized using negative ionization mode, and its validation test was completed for solid and liquid samples. In addition, KASP markers were prepared by finding the specific SNP of G. sylvestre in ITS2 and matK through DNA barcodes. The two KASP markers returned positive FAM fluorescence result when combined with G. sylvestre, and this aspect was confirmed in raw G. sylvestre as well. The applicability of the method was tested on 21 different food and healthy functional products containing G. sylvestre purchased on the internet. As a result, although there was a difference in the ratios of gymnemic acid and deacylgymnemic acid in LC-ESI-MS/MS, the index component was detected in all 21 products samples. In the KASP analysis, 9 products returned positive FAM result, and the rest of the products were found to be containing G. sylvestre extract. This study is the first study to use the dual system of LC-ESI-MS/MS and KASP for the analysis of G. sylvestre. The study has confirmed that these two methods are applicable to the examine G. sylvestre content in food products.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.97-106
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• 2017

Living modified crops are genetically modified living organisms and are widely used in biotechnical research and desired goods. As the reliance on LM products, concerns about safety of LMOs have been continuously increased in South Korea. We established the detection methods for unintentional released LMOs in environmental conditions. To detect six LM event genes of 1 canola, 1 maize and 4 soybeans, PCR conditions were based upon consideration of the Joint Research Centre information. Genomic DNAs were isolated from LM samples and PCR analysis were performed using each event-specific primer pair. Event-specific genes of all events were efficiently recognized by our methods. To investigate the insertion site of LM genes in each genome, we verified PCR product sequence by DNA sequencing. These results suggest that the LM event-specific gene amplification can be efficiently developed. In addition, our detection method is fit for monitoring and post-management of LM crops in the environment.

• Korean journal of applied entomology
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• v.20 no.2 s.47
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• pp.117-121
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• 1981

The screening method of varietal resistance on the plant hoppers has generally been evaluated as a reaction of plant after infesting insects. However, feeding amount of insects to the varieties was investigated in this experiment. The new method using isotope $^{32}P$ for rice varietal resistance to plant hoppers was carried out through the following method. Insects tested were caged for a few hours on the plants which had absorbed $^{32}P$ solution in small vials for $24\~48\;hours$. After feeding, insects were killed in the refrigerator with formalin solution, and then were measured by the feeding amount as a count per minute (CPM) with the G.M. Counter. The results obtained were summarized as follows; 1. The apparatus of Type D(Fig. 2) was most effective and the safest among four others. 2. The optimum amount of $H_3PO_4$ solution was found to be $2\~3m1$. 3. Radioactivity of $0.7\mu\;Ci.\;^{32}P$ was sufficient to check varietal difference of feeding amount by the brown planthopper. 4. Radioisotope was found from the body of insects but not in the cuticular layer nymphs cast off.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.6
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• pp.958-964
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• 2002

To select the irradiation-induced marker components from volatile flavor compounds in irradiated chicken, and complement the extraction problems of liquid continuous extraction (LLCE) method, the volatile compounds of irradiated (0,1,3,5 and 10 kGy) chicken were analyzed by Purge and Trap (P&T) and gas chromatography/mass spectrometry (GC/MS) methods. A total of 119 compounds were detected in irradiated chicken, and these compounds were composed mainly of 7 aldehydes,22 ketones,8 alcohols,30 esters,36 hydrocarbons,8 aromatic compounds and 8 miscellaneous compounds. Among these, only 21 compounds were detected in both LLCE and P&T methods, and the 98 other were detected in omly P&T method. Among volatile compounds detected in irradiated chicken, only 3 compounds such as hexene (r=0.96, p<0.01), propanol (r=0.93, p<0.05) and 1,3-bis(1,1-dimethylethyl) benzene (r=0.96, p<0.05) were newly selected as marker compounds in irradiated chicken by P&T method, which showed significant and high positive correlation coefficient in the change of relative concentration according to the increment of irradiation dosage.