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http://dx.doi.org/10.5010/JPB.2017.44.1.097

Development of detection methods for six approved LM crops in Korea  

Seol, Min-A (Division of Ecological Conservation, National Institute of Ecology (NIE))
Jo, Beom-Ho (Bio-industrial Team, Bio Center, Chungnam Techno Park)
Choi, Wonkyun (Division of Ecological Conservation, National Institute of Ecology (NIE))
Shin, Su Young (Division of Ecological Conservation, National Institute of Ecology (NIE))
Eum, Soon-Jae (Division of Ecological Conservation, National Institute of Ecology (NIE))
Kim, Il Ryong (Division of Ecological Conservation, National Institute of Ecology (NIE))
Song, Hae-Ryong (Division of Ecological Conservation, National Institute of Ecology (NIE))
Lee, Jung Ro (Division of Ecological Conservation, National Institute of Ecology (NIE))
Publication Information
Journal of Plant Biotechnology / v.44, no.1, 2017 , pp. 97-106 More about this Journal
Abstract
Living modified crops are genetically modified living organisms and are widely used in biotechnical research and desired goods. As the reliance on LM products, concerns about safety of LMOs have been continuously increased in South Korea. We established the detection methods for unintentional released LMOs in environmental conditions. To detect six LM event genes of 1 canola, 1 maize and 4 soybeans, PCR conditions were based upon consideration of the Joint Research Centre information. Genomic DNAs were isolated from LM samples and PCR analysis were performed using each event-specific primer pair. Event-specific genes of all events were efficiently recognized by our methods. To investigate the insertion site of LM genes in each genome, we verified PCR product sequence by DNA sequencing. These results suggest that the LM event-specific gene amplification can be efficiently developed. In addition, our detection method is fit for monitoring and post-management of LM crops in the environment.
Keywords
Living modified organism (LMO); Monitoring; Detection method; Polymerase chain reaction (PCR);
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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1 Kim JH, Park SB, Roh HJ, Park SH, Shin MK, Moon GI, Hong JH, Kim HY (2015) A simplified and accurate detection of the genetically modified wheat MON71800 with one calibrator plasmid. Food Chem. 176:1-6   DOI
2 Kim JH, Seo YJ, Sun SH, Kim HY. Multiplex PCR detection of 4 events of genetically modified soybeans(RRS, A2704-12, DP356043-5, and MON89788) (2009b) Food Sci. Biotechnol. 18(3):694-699
3 Lee BK, Kim CG, Park JY, Park KW, Kim HJ, Yi H, Jeong SC, Yoon WK, Kim HM (2009) Monitoring the occurrence of genetically modified soybean and maize in cultivated fields and along the transportation routes of Incheon Port in South Korea. Food con. 250-254
4 Lee JR, Shin SY, Choi WK, Moon JC, Jo BH, Seol MA, Eum SJ, Kim IR, Kim JM, Song HR (NIE) (2015c) Study on environmental monitoring and post-management of LMO (VII)
5 Lee JR, Choi WK, Moon JC, Jo BH, Shin SY, Seol MA, Eum SJ, Kim IR, Song HR, Kim JM (NIE) (2015a) LMO detection methods. ISBN 979-11-86197-24-0
6 Lee JR, Jo BH, Choi WK, Moon JC, Shin SY, Eum SJ, Seol MA, Kim IR, Kim JM, Song HR (NIE) (2015b) Development of multiplex-PCR for simultaneous detection of LMOs (III)
7 Lee SH, Kim JK, Yi BY. Detection methods for biotech cotton mon15985 and mon88913 by PCR (2007) J. Agric Food Chem. 55:3351-3357   DOI
8 Ministry of agriculture food and rural affairs. Self-sufficiency rate of grain in Korea. Available from: http://www.mafra.go.kr. Accessed 2015
9 Min DM, Kim MY, Jung SI, Heo MS, Kim JK, Kim HY (2004) Quantitative analysis of genetically modified soybean in processed foods using real-time PCR. Korean J. Food Sci. Technol. 36(5): 723-727
10 Mazzara M, Charles Delobel C, Pinski G, Savini C, Van den Eede G (2012) Event-specific Method for the Quantification of Soybean MON87769 Using Real-time PCR. Validation Report and Validated Method. EUR 25487 EN.2012. JRC74371
11 Moon GS, Shin WS. Establishment of quantitative analysis method for genetically modified maize using a reference plasmid and novel primers (2012) Prev Nutr Food Sci. 17:274-279   DOI
12 Park KW, Lee BK, Kim CG, Kim DY, Park JY, Ko EM, Jeong SC, Yoon WK, Kim HM (2010) Monitoring of occurrence of genetically modified maize at a grain receiving port and along transportation routes in the Republic Korea. Food Con. 456-461
13 Savini, C. Event-specific Method for the Quantification of Oilseed Rape MON88302 Using Real-time PCR-Validation Report and Validated Method (2013a) JRC86264
14 Savini C, Bogni A, Foti N, Mazzara M, Kreysa J. Event-specific Method for the Quantification of Maize DAS-40278-9 Real-time PCR-Validation Report and Validated Method (2012) EUR 25585 EN
15 Savini C, Mazzara M, Munaro B, Kreysa J. Event-specific Method for the Quantification of Soybean MON87708 Using Real-time PCR-Validation Report and Validated Method (2013b) EUR 25982 EN. JRC81896
16 Savini C, Sacco MG, Mazzara M, Kreysa J. Event-specific Method for the Quantification of Soybean DAS-68416-4 Using Realtime PCR-Validation Report and Validated Method (2014) EUR 26706 EN. JRC 89867
17 Wu G, Wu YH, Xiao L, Lu CM. Event-specific qualitative and quantitative PCR detection of genetically modified rapeseed topas 19/2 (2009) Food Chem. 112:232-238   DOI
18 Savini C, Sacco MG, Mazzara M, Kreysa J. Event-specific Method for the Quantification of Soybean DAS-44406-6 Using Real-time PCR-Validation Report and Validated Method (2015) JRC 95249
19 Seralini GE, Clair E, Mesnage R, Gress S, Defarge N, Malatesta M, Hennezuin D, Joel Spiroux de Vendomois (2012) Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize. Food and Chemical Toxicology 50:4221-4231   DOI
20 Seol MA, Lee JR, Choi WK, Jo BH, Moon JC, Shin SY, Eum SJ, Kim IR, Song HR (2015) Establishment of detection methods for approved LMO in Korea. J Plant Biotechnol. 42:196-203   DOI
21 Yoo MR, Kim JH, Yea MC, Kim HY (2013) Development of detection method of unapproved genetically modified potato (EH92-527-1) in Korea using duplex polymerase chain reaction. Korean J. Food Sci. 45(2):156-160   DOI
22 Kim JH, Kim HY (2009a) Event-specific detection methods for genetically modified maize MIR604 using real-time PCR. Food Sci. Biotechnol. 18(5):1118-1123
23 Agbios (2001) Esential Biosafety. Vol. 1 No. 1 Merkvile, Canada
24 Choi WK, Seol MA, Lee JR, Jo BH ,Moon JC, Shin SY, Eum SJ, Kim IR, Kim JM, Song HR (NIE) (2015) Establishment of detection methods for LMO
25 Han SM, Kim DY, Md RU, Hwang KS, Lee BK, Kim CG, Park KW (2014) Appearance/Instance of genetically modified maize at grain receiving harbors and along transportation routes in Korea. Weed Turf. Sci 3:221-224   DOI
26 Han SM, Oh TK, Md RU, Shinogi Y, Lee BK, Kim CG, Park KW (2015) Monitoring the occurrence of genetically modified maize in Korea: A 3-year observations. J Fac Agriculture, 60(2):285-290
27 European Commission, Joint Research Centre (JRC). Available from: http://gmo-crl.jrc.ec.europa.eu
28 International Service for the Acquisition of Agri-biotech Applications (ISAAA). GM Approval Database. Available from: http://www.isaaa.org. Accessed 2015
29 Korea Biosafety Clearing House(KBCH). Status of risk assessment of GMO in Korea. Available from: http://www.biosafety.or.kr. Accessed 2015
30 Kim JH, Kim SA, Seo YJ, Lee WY, Park SH, Kim HY (2008) Multiplex PCR detection of the MON1445, MON15985, MON88913, and LLcotton25 Varieties of GM cotton. Food Sci. Biotechnol. 17(4):829-832