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Genetic Diversity and Identification of Korean Elite Soybean Cultivars including Certified Cultivars Based on SSR Markers  

Jang, Seong-Jin (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Park, Su-Jeong (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Park, Kyeong-Ho (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Song, Hang-Lin (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Cho, Yong-Gu (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Jong, Seung-Keun (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Kang, Jung-Hoon (National Academy of Agricultural Science, RDA)
Kim, Hong-Sig (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.54, no.2, 2009 , pp. 231-240 More about this Journal
Abstract
A total of 26 Korean elite soybean cultivars including 21 certified cultivars was assessed to evaluate genetic diversity and to analyze relationship among them based on 15 SSR markers. Fifteen SSR markers generated a total of 201 alleles. Average number of alleles per SSR marker was 13.4 with a range from 8 to 19. Genetic diversity of 26 cultivars estimated by PIC value ranged from 0.782 to 0.931 with an average of 0.874. PIC value of Satt197 was the highest with 0.931 and Satt141 was the lowest with 0.782 among 15 SSR markers. Cluster analysis based on genetic distances classified 26 soybean cultivars into 3 clusters. Cluster I, II and III included 2, 7 and 17 cultivars, respectively. Average genetic diversity within clusters was 0.769 with a range from 0.720 to 0.799. Average genetic diversity between clusters was 0.813 with a range from 0.725 to 0.857. Genetic diversity was higher between clusters than within clusters. Genetic relationship among clusters was near between I and II, and I and III and far between II and III cluster. All of 26 Korean elite soybean cultivars could be identified by using any of 5 combinations of 2 SSR markers with higher PIC value, i.e, $Satt197+Sat_088$, Satt197+Satt245, $Sat_088+Sat_-036$, $Sat_088+Satt245$ and Satt185+Satt245.
Keywords
Genetic Diversity; Genetic Relationship; Identification; SSR; Korean Elite Soybean Cultivar; Certified Cultivar;
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1 Abe, J., D. H. Xu, Y Suzuki, aud A. Kanazawa. 2003. Soybean germplasm pools in asia revealed by nuclear SSRs. Theor. Appl. Genet. 106 : 445-453   DOI   PUBMED
2 Brown-Guedira, G. L., J. A. Thompson, R. L. Nelson, and M. L. Warburton. 2000. Evaluation of genetic diversity of soybean introductions and North American ancestors using RAPD and SSR markers. Crop Sci. 40 : 815-823   DOI   ScienceOn
3 Jong, S. K., H. S. Kim, and S. Y Son. 1999. Genetic diversity using pedigree analysis in Korean soybean varieties. Korean J. Breed. 31(4) : 313-322
4 Kim, S. H., J. W. Chung, J. K. Moon, S. H. Woo, Y. G. Cho, S. K. Jong, and H. S. Kim. 2006. Genetic diversity and relationship by SSR markers of korean soybean cultivars. Korean J. Crop Sci. 51(3) : 249-258
5 Li, C. D., C. A. Fatokun, B. Ubi, B. B. Singh, and G. J. Scoles. 2001. Determing genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers. Crop Sci. 41 : 189-197   DOI   ScienceOn
6 Rongwen, J. M., S. Akkaya, A. A. Bhagwat, U. Lavi, and P. B. Cregan. 1995. The use of microsatellite DNA markers for soybean genotype identification. Theor. Appl. Genet. 90 : 43-48
7 Satoh, H., S. Sawada, and S. Itoh. 2003. Rate of acceptance and longevity of pulse and wheat cultivars in Hokkaido. Jpn. J. Crop Sci. 72(4) : 418-423   DOI   ScienceOn
8 Song, Q. J., C. V. Quigley, R. L. Nelson, T. E. Carter, H. R. Boerma, J. L. Strachan, and P. B. Cregan. 1999. A selected set of trinucleotide simple sequence repeat markers for soybean cultivar indentification. Plant Varieties and Seeds 12 : 207-220
9 Van, K. J., M. Y Kim, J. G. Gwag, K. G. Bea, Y J. Oh, K. H. Kim, H. K. Park, and S. H. Lee. 2003. Genetic variation in sprout-related traits and micro satellite DNA loci of soybean. Korean J. Crop Sci. 48(5) : 413-418
10 Wang, L., R. Guan, L. Zhangxiong, R. Chang, and L. Qiu. 2006. Genetic diversity of Chinese cultivated soybean revealed by SSR markers. Crop Sci. 46 : 1032-1038   DOI   ScienceOn
11 Yoon, M. S., J. R. Lee, H. J. Beak, G. T. Cho, C. Y Kim, Y H. Cho, T. S. Kim, and E. G. Cho. 2007. SSR profiling and its variation in soybean germplasm. Korean J. Crop Sci. 52(1) : 81-88
12 National Institute of Crop Science, RDA. 2008. Compendium of legume cultivars (Soybean, Adzuki bean, Mung bean, Pea, Common bean, Cowpea). pp. 842
13 Li, Z. and R. L. Nelson. 2001. Genetic diversity among soybean accessions from three countries measured by RAPDs. Crop Sci. 41 : 1337-1347   DOI   ScienceOn
14 Nichols, D. M., W. Lianzheng., Y Pei, K. D. Glover, and B. W. Diers. 2007. Variability among Chinese Glycine soja and Chinese and North American soybean genotypes. Crop Sci. 47 : 1289-1298   DOI   ScienceOn
15 Zhou, X., E. Thomson, Jr. Carter, Z. Cui, S. Miyazaki, and J. W. Burton. 2002. Genetic diversity patterns in Japanese soybean cultivars based on cofficient of parentage. Crop Sci. 42 : 1331-1342   DOI   ScienceOn
16 Cregan, P. B., T. Jarvik, A. L. Bush, R. C. Shoemaker, K. G. Lark, A. L. Kahler, N. Kaya, T. T. VanToai, D. G. Lohnes, J. Chung, and J. E. Specht. 1999. An integrated genetic linkage map of the soybean genome. Crop Sci. 39 : 1464-1490   DOI
17 Chen, Y and R. L. Nelson. 2004. Genetic variation and relationships among cultivated, wild and semiwild soybean. Crop Sci. 44 : 316-325   DOI   ScienceOn
18 Hwang, Y. H. 2004. Historical review on soybean cultivation in korea. International symposium on the development of functional soybean varieties, New Materials, Medicine, and Foods. 1-29. Kyungbuk National University
19 Hwang, T. Y, Y. Nakamoto, I. Kono, H. Enoki, H. Funatsuki, K. Kitamura, and M. Ishimoto. 2008. Genetic diversity of cultivated and wild soybeans including Japanese elite cultivars as revealed by length polymorphism of SSR markers. Breeding Science 58 : 315-323   DOI   ScienceOn
20 Lee, J. D., J. K. Yu, Y. H. Hwang, S. Blake, Y. S. So, G. J. Lee, H. T. Nguyen, and J. G. Shannon. 2008. Genetic diversity of wild soybean (Glycine soja Sieb. and Zucc.) accessions from South Korea and other countries. Crop Sci. 48 : 606-616   DOI   ScienceOn
21 Tanya, P., P. Srinives, T. Toojinda, A. Vanavichit, B. K. Ha, J. S. Bae, J. K. Moon, and S. H. Lee. 2001. Evaluation of genetic diversity among soybean genotypes using SSR and SNP. Korean J. Crop Sci. 46(4) : 334-340
22 Turuspekov, Y, K. Nakamura, R. Yoshikawa, and R. Tuberosa. 2001. Genetic diversity of Japanese barley cultivars based on SSR analysis. Breeding Science 51 : 215-218   DOI   ScienceOn
23 Narvel, J. M., W. R. Fehr, W. C. Chu, D. Grant, and R. C. Shoemaker. 2000. Simple sequence repeat diversity among soybean plant introductions and elite genotypes. Crop Sci. 40 : 1452-1458   DOI   ScienceOn
24 Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sci. USA 70(12) : 3321-3323   DOI   ScienceOn
25 Yeh, F. C., R. C. Yang, T. B. J. Boyle, Z. H. Ye, and J. X. Mao. 1997. POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada. 1-28
26 Bommi, P. and D. L. Ferguson. 2005. Soybean cultivar identification within a selected group using only an agarose gel system with simple sequence repeat DNA markers. Soybean Genetics Newsletter. 32 : 1-5
27 Fu, Y. B.., G. W. Peterson, and M. J. Morrison. 2007. Genetic diversity of Canadian soybean cultivars aud exotic germplasm revealed by simple sequence repeat markers. Crop Sci. 47 : 1947-1954   DOI   ScienceOn
28 Cho, Y. G., T. Ishii, S. M. Temnykh, X. Chen, L. Lipovich, S. R. McCouch, W. D. Park, N. Ayres, and S. Cartinhour. 2000. Diversity of microsatellites derived from genomic libraries and genebank sequences in rice (Oryza sativa L.). Theor. Appl. Genet. 100 : 249-257   DOI   ScienceOn
29 Cui, Z., T. E. Carter, and J. W. Burton. 2000. Genetic diversity patterns in Chinese soybean cultivars based on coefficient of parentage. Crop Sci. 40 : 1780-1793   DOI   ScienceOn
30 Burnham, K. D., D. M. Francis, A. E. Dorrance, R. J. Firitto, and S. Kt. martin. 2002. Genetic diversity patterns among phytophthora resistant soybean plant introductions based on SSR markers. Crop Sci. 42 : 338-343   DOI   ScienceOn
31 Park, K. Y, Y. H. Lee, S. D. Kim, and E. H. Hong. 2000. Review and future planning for soybean breeding in Korea. Korea Soybean Digest. 17(1) : 13-26
32 Kim, S. H., J. W. Chung, J. K. Moon, S. H. Woo, Y G. Cho, S. K. Jong, and H. S. Kim. 2006. Discrimination of Korean soybean cultivars by SSR markers. Korean J. Crop Sci. 51(7) : 1-11   과학기술학회마을
33 Korea seed & variety service. 2009. http://www.seed.go.kr