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Studies on Recovery and Cryopreservation of Embryos in Korean Native Swine (재래돼지에서 수정란의 회수 및 동결보존에 관한 연구)

  • Son D. S.;Yeon S. H.;Hur T. Y.;Kang S. J.;Suh G. H.;Choi S. H.;Ryu I. S.;Lee K. S.;Park C. S.
    • Journal of Embryo Transfer
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    • v.19 no.3
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    • pp.257-264
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    • 2004
  • For safe preservation of Korean Native Pigs (KNP) as an animal genetic resources and a means to maintain the genetic diversity, we performed to investigate the optimal hormone levels for superovulated gilts and establish the cryopresevation methods of embryos. The reults were as follows; 1. The number of ovulated corpus luteums (CL) and follicles were 12.4, 13.6, 30.0 or 23.3 in hCG 500IU and PMSG 500, 750, 1,000IU or hCG 750IU and PMSG 1,000IU respectively. In the case of PMSG 1,000IU와 hCG 500IU, there showed highest number but were no significance among others. The recovery rate of embryos by the ovulated CL were 59.4-79.2%. 2. The morula stage embryos recovered on Day 4 after insemination were significantly higher than Day 5 (P<0.01), but blastocyst stage embryos recovered on Day 5 were sinificantly higher than Day 4 (P<0.05). 3. The survival rate of expanded blastocyst were 23.5% in conventional freezing with 1.4 M glycerol.

Effects of Some Factors on In Vitro Production of Embryos from Antral Follicle-Derived Porcine Oocytes IV. Effects of Development Media and Those Change on In Vitro Development (돼지 난포란 유래 체외수정란 생산에 대한 제요인의 영향 IV. 체외발달 배양액의 종류와 배양액 교체가 체외발달에 미치는 영향)

  • Yeon S. H.;Choi S. H.;Cho C. Y.;Han M. H.;Son D. S.;Lee K. S.
    • Journal of Embryo Transfer
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    • v.19 no.3
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    • pp.275-282
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    • 2004
  • This study was carried out to examine the effects of development media and those change on in vitro development (IVD) of porcine oocytes matured and fertilized in vitro. Putative embryos, which were matured in vitro in modified NCSU-23 (mNCSU-23) supplemented with 10% porcine follicular fluid (pFF) and were fertilized in mTBM, were developed in vitro as the experimental scheme. The results are as follows. When porcine putative embryos were cultured in vitro in NCSU-23 or CZB/Pig-MEM, the percentage of oocytes cleaved was not different between two systems, but the percentage of blastocysts in NCSU-23 was significantly higher than in CZB/Pig-MEM (P<0.05). And when porcine putative embryos were cultured in vitro in NCSU-23 during 7 days with or without changing media at day 5, which was supplement with or without 10% fetal bovine serum(FBS), the percentage of oocytes cleaved, blastocysts at day 6, and the cell number of ICM, TE and total of blastocysts at day 7 were not different among three treatments. As a result of this study, it is supposed that NCSU-23 be more favorable, to develop porcine embryos derived from IVM/IVF, than CZB/Pig-MEM, but that demonstration on the effects of changing medium with fresh one stand in need of the more experiments.

Determination of ibuprofen and its metabolites in human urine by GC-MS (GC-MS에 의한 소변 중 Ibuprofen의 대사체 규명 및 대사 연구)

  • Yu, Dae-Hyung;Cho, Jung-Hum;Hong, Jong-Ki
    • Analytical Science and Technology
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    • v.23 no.2
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    • pp.179-186
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    • 2010
  • The oxidative metabolism of ibuprofen in healthy male urine collected at 3, 6, 9, 12 and 15 h after oral administration of ibuprofen was studied by GC/MS assay. To detect conjugated metabolites of ibuprofen, urine sample was acid-hydrolyzed with 6 M HCl at $100^{\circ}C$ for 30 min. To effectively extract ibuprofen and its metabolites, liquid-liquid extraction (LLE) was conducted at pH 3, 5, and 7, respectively. As a result, LLE at pH 3 was shown to be the best extraction condition. For the determination of trace amounts of ibuprofen and its metabolites in extract, trimethylsilylation (TMS) with BSTFA was applied and followed by GC/MS analysis. In this study, main 5 metabolites including parent drug were detected and these metabolites were assigned as three hydroxylated forms and one carboxylated form. Each metabolite was tentatively identified by both interpretation of mass spectrum and comparison with previously reported results. In addition, time profile of urinary excretion rate for parent drugs and metabolites was studied. Finally, the metabolic pathways of ibuprofen were suggested on the basis of the structural elucidation of its metabolites and excretion profiles.

Comparative Ultranstructures of the Fertilized Egg Envelopes from Three-spot gourami, Pearl gourami and Marble gourami, Belontiidae, Teleost (경골어류 등목어과 Three-spot gourami, Pearl gourami 및 Marble gourami의 수정란 난막 미세구조 비교)

  • Kim, Dong-Heui;Deung, Young-Kun;Kim, Wan-Jong;Reu, Dong-Suck;Kang, Song Jian
    • Applied Microscopy
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    • v.29 no.3
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    • pp.343-351
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    • 1999
  • The structures of the fertilized egg envelope from three species, three-spot gourami (Trichogaster trichopterus), pearl gourami (Trichogaster leeri) and marble gourami (Trichogaster trichopterus trichopterus) belong to Belontiidae were observed, utilizing light, scanning and transmission electron microscopes. In all three species, the fertilized eggs were the colorless, transparent, spherical, adhesive and pelagic type. A large oil droplet was located in vitelline membrane of the fertilized egg. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. Specially, the micropyle of three-spot gourami was similar to that of marble gourami which is subspecies of three-spot gourami. An outer surface of the fertilized egg envelope was arranged by grooves in all three species. The fertilized egg envelopes consists of two distinct layers; an adhesive outer layer and an inner layer with high electron density. In conclusion, the morphological similarity of the fertilized egg, micropyle, outer surface and transverse section of the fertilized e9g envelope seems to be an indication of the Belontiidae.

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Genetic Composition of Korean Native Chicken Populations - National Scale Molecular Genetic Evaluation Based on Microsatellite Markers (초위성체 표지로 본 한국 재래닭 집단의 분자유전학적 구성)

  • Lee, Poong-Yeon;Yeon, Seong-Heum;Kim, Jae-Hwan;Ko, Yeoung-Gyu;Son, Jun-Kyu;Lee, Hee-Hoon;Cho, Chang-Yeon
    • Korean Journal of Poultry Science
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    • v.38 no.2
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    • pp.81-87
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    • 2011
  • The study was conducted to select and optimize microsatellite (MS) markers for evaluate Korean Native Chicken (KNC) breeds in order to provide standard for the classification and breed definition of the indigenous breeds. The study also aimed to characterize and classify each KNC populations for inventory and management of avian genetic resources. A total of 462 chickens from 11 populations of chicken breeds including eight KNC breeds and three commercial chicken breeds were analyzed with 19 MS markers. KNC breeds, especially Long-Tail Chicken breeds, formed separate cluster from those commercial chicken breeds. Genetic distances between KNC populations (0.11~0.18) were relatively shorter. Genetic uniformity of KNC (except KNCR breed) (0.86~0.88) were higher than that of commercial breeds (except Cornish) (0.95~0.97). On the other hand, genetic uniformity of KNC Long Tail (KNCLT) were relatively higher (0.91~0.97). The result can be used to evaluate and manage animal genetic resources at national scale.

A Study on the Optimization of New Renewable Energy Systems in Public-Purpose Facilities (공공용 업무시설의 신재생에너지시스템 최적화 연구)

  • Lee, Yong-Ho;Seo, Sang-Hyun;Cho, Young-Hum;Hwang, Jung-Ha
    • Journal of the Korean Solar Energy Society
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    • v.33 no.5
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    • pp.95-104
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    • 2013
  • This study set out to devise an optimized system to take into account life cycle cost(LCC) and ton of carbon dioxide($TCO_2$) by applying the weighted coefficient method(WCM) to "public-purpose" facility buildings according to the mandatory 5% and 11% of new renewable energy in total construction costs and anticipated energy consumption, respectively, based on the changes of the public obligation system. (1) System installation capacity is applied within the same new renewable energy facility investment according to the mandatory 5% of new renewable energy in total construction costs. Both LCC and $TCO_2$ recorded in the descending order of geothermal, solar, and photovoltaic energy. The geothermal energy systems tended to exhibit an excellent performance with the increasing installation capacity percentage. (2) Optimal systems include the geothermal energy(100%) system in the category of single systems, the solar energy(12%)+geothermal energy(88%) system in the category of 2-combined systems, and the photovoltaic energy(12%)+solar energy(12%)+geothermal energy(76%) system and the photovoltaic energy(12%)+solar energy(25%)+geothermal energy(63%) system in the category of 3-combined systems. (3) LCC was the highest in the descending order of photovoltaic, geothermal and solar energy due to the influences of each energy source's correction coefficient according to the mandatory 11% of new renewable energy in anticipated energy consumption. The greater installation capacity percentage photovoltaic energy had, the more excellent tendency was observed. $TCO_2$ recorded in the descending order of geothermal, photovoltaic and solar energy with the decreasing installation capacity of photovoltaic energy. The greater installation capacity percentage a geothermal energy system had, the more excellent tendency it demonstrated. (4) Optimal systems include the geothermal energy(100%) system in the category of single systems, the photovoltaic energy(62%)+geothermal energy(38%) system in the category of 2-combined systems, and the photovoltaic energy(50%)+solar energy(12%)+geothermal energy(38%) system and the photovoltaic energy(12%)+solar energy(12%)+geothermal energy(76%) system in the category of 3-combined systems.

Modeling of Medium Temperature Drops of the Elevated-bench Hydroponics for Strawberry Cultivation during Low Temperature Season (저온기 딸기 고설 수경재배시 온실기온에 따른 배지내 온도강하 모델 개발)

  • Park, Jae-Wan;Ha, Yu-Shin;Kim, Ki-Dong;Park, Dae-Heum;Lee, Ki-Myung;Jun, Ha-Joon;Kwon, Soon-Gu;Choi, Won-Sik;Chung, Sung-Won
    • Journal of Bio-Environment Control
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    • v.19 no.3
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    • pp.123-129
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    • 2010
  • A study on modeling of medium temperature drops of the elevated-bench hydroponic system for strawberry cultivation during low temperature season was conducted. Four different conditions were used for the experiment. These consisted of two kinds of bed types (plant, V), four kinds of medium (rice, perlite, rice hulls80% and peatmoss20%, perlite80% and peatmoss20%), two kinds of mulched bed (mulched, non mulched) and four kinds of greenhouse air temperature (l.5, 3.2, 5.0, $6.7^{\circ}C$), and the results were summarized as follows: Temperature drop of medium in the V-bed was slower than that in the plant bed, showing better insulation effect of V-bed. Temperature drop of medium with mulching on the top of the bed was slower than the case without mulching, as a result, the beneficial effect of temperature drop was appeared in mulched bed. Linear regression of the temperature descent rate and the temperature difference between medium and air showed significant correlation. The regression equation for the Pearlite80% and Peatmoss20% in the V-bed was f(x) = -0.2656 + 0.1345x at the $R^2$ of 0.9269. Using the model, the temperature drop during night can be predicted for the various media at the different depths.

LiF TLD in TLD Holder for In Vivo Dosimetry (생체 내 선량측정을 위한, TLD홀더에 넣은 LiF TLD)

  • Kim Sookil;Loh John J.K.;Min Byungnim
    • Radiation Oncology Journal
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    • v.19 no.3
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    • pp.293-299
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    • 2001
  • Prupose : LiF TLD has a problem to be used in vivo dosimetry because of the toxic property of LiF. The aim of this study is to develop new dosimeter with LiF TLD to be used in vivo dosimetry. Materials and methods : We designed and manufactured the teflon box(here after TLD holder) to put TLD in. The external size of TLD holder is $4\times4\times1\;mm^3$ To estimate the effect of TLD holder on TLD response for radiation, the linearity of TLD response to nominal dose were measured for TLD in TLD holder. Measurement were peformed in the 10 MV x-ray beam with LiF TLD using a solid water phantom at SSD of 100 cm. Percent Depth Dose (PDD) and Tissue-Maximum Ratio (TMR) with varying phantom thickness on TLD were measured to find the effect of TLD holder on the dose coefficient used for dose calculation in radiation therapy. Results : The linearity of response of TLD in TLD holder to the nominal dose was improved than TLD only used as dosimeter And in various measurement conditions, it makes a marginnal difference between TLD in TLD holder and TLD only in their responses. Conclusion : It was proven that the TLD in TLD holder as a new dosimetry could be used in vivo dosimetry.

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The Philosophical Significance of Contemporary China's Reconstruction Movement of the Traditional Thought and Culture and its Future (당대(當代) 중국(中國)의 전통문화(傳統文化) 복원운동(復原運動)의 철학적 함의와 그 전망)

  • Yeon, Jae-heum
    • The Journal of Korean Philosophical History
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    • no.30
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    • pp.311-344
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    • 2010
  • Over 100 years in Mainland China, the 'traditional' thought and culture have been transformed from 'the past must be sublated' to 'the heritage must be preserved.' Today, China hopes to deal with the negative effects of industrial development such as the gap between rich and poor, and the absence of common values etc. through the reconstruction of traditional thought and culture, especially that of Confucianism. The Chinese government superficially accepts Confucianism for the purpose of the social stabilization and ongoing economic development while they keep the Socialism as the nation's ideological system. In the private sectors, there is endeavoring move to make the Chinese traditional thought and culture global paralleled to their economic growth. There are also scholars who find the cultural unification and the spiritual comfort from the restoration of the traditional thought and culture. They, so called 'the critical successionists' or 'the cultural conservativists,' believe that the traditional thought and culture could play a role in the Chinese modernization, and the new understanding and interpretation of their tradition could provide an alternative for their future. In my view, we need a careful survey on the view of the critical successionists or the cultural conservativists who regard tradition and culture as a power (力量). With this, we are required to investigate our own problems through the new understanding of our traditional thought and culture.

Oral hygiene management of patients with dental implants using electronic media (Smartphone) (전자매체(스마트폰)를 이용한 치과임플란트환자의 구강위생 관리)

  • Yang, Hyun Woo;Kim, Jin;Choi, Hanmaeum;Fang, Yiqin;Kim, So Young;Lee, Chunui
    • Journal of Korean Academy of Dental Administration
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    • v.7 no.1
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    • pp.39-43
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    • 2019
  • Smartphone usage has become so common that it has reached 2 billion people in the last year. As a result of this, hospitals have started making use of smartphones at various medical sites and research services for patients. This study aimed to establish support for developing a long distance program for patients with implants who have difficulty visiting clinics or with busy modern lives, by using smartphones for oral hygiene management instruction. The data were collected for 12 weeks, from July 24 to October 21, 2015, for patients who agreed to participate in the study. Although the subjects found the process of transferring photos via smartphone to be cumbersome (75%), the satisfaction level of the oral hygiene management program was excellent for all participating patients, and they all wanted to continue with further management using this process. The results from the phone satisfaction survey showed that oral hygiene self-management after oral hygiene control training by smartphones was mostly equal to previous habits (87.5%) or had partially increased but had not decreased. The need for data on more varied age groups and the issues of protecting the security of personal information on smartphones require further study. However, our study confirmed the efficacy of using electronic media (smartphones) for oral hygiene management in patients with a dental implant due to their improvement of oral hygiene performance as evidenced by less bleeding from probing on post-program visit.