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Studies on Recovery and Cryopreservation of Embryos in Korean Native Swine  

Son D. S. (농촌진흥청 축산연구소)
Yeon S. H. (농촌진흥청 축산연구소)
Hur T. Y. (농촌진흥청 축산연구소)
Kang S. J. (농촌진흥청 축산연구소)
Suh G. H. (농촌진흥청 축산연구소)
Choi S. H. (농촌진흥청 축산연구소)
Ryu I. S. (농촌진흥청 축산연구소)
Lee K. S. (충남대학교 동물자원학부)
Park C. S. (충남대학교 동물자원학부)
Publication Information
Journal of Embryo Transfer / v.19, no.3, 2004 , pp. 257-264 More about this Journal
Abstract
For safe preservation of Korean Native Pigs (KNP) as an animal genetic resources and a means to maintain the genetic diversity, we performed to investigate the optimal hormone levels for superovulated gilts and establish the cryopresevation methods of embryos. The reults were as follows; 1. The number of ovulated corpus luteums (CL) and follicles were 12.4, 13.6, 30.0 or 23.3 in hCG 500IU and PMSG 500, 750, 1,000IU or hCG 750IU and PMSG 1,000IU respectively. In the case of PMSG 1,000IU와 hCG 500IU, there showed highest number but were no significance among others. The recovery rate of embryos by the ovulated CL were 59.4-79.2％. 2. The morula stage embryos recovered on Day 4 after insemination were significantly higher than Day 5 (P<0.01), but blastocyst stage embryos recovered on Day 5 were sinificantly higher than Day 4 (P<0.05). 3. The survival rate of expanded blastocyst were 23.5％ in conventional freezing with 1.4 M glycerol.
Keywords
cryopreservation; Korean Native Pig(KNP);
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