• Journal of Life Science
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• v.22 no.2
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• pp.142-147
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• 2012

The sporulation-specific glucoamylase (SGA) of Saccharomyces diastaticus is known to be produced in the cytoplasm during sporulation. For the purpose of proving that SGA has secretory potential, we constructed a hybrid plasmid, pYESC25, containing the promoter and the putative signal sequence of the SGA fused in frame to the endo-1,4-${\beta}$-D-glucanase (CMCase) gene of Bacillus subtilis without its own signal sequence. The recipient yeast strain of S. diastaticus YIY345 was transformed with the hybrid plasmid. CMCase secretion from S. diastaticus harboring pYESC25 into culture medium was confirmed by the formation of yellowish halos around transformants after staining with Congo red on a CMC agar plate. The transformant culture was fractionated to the extracellular, periplasmic, and intracellular fraction, followed by the measurement of CMCase activity. About 63% and 13% enzyme activity were detected in the culture supernatant (extracellular fraction) and periplasmic fraction, respectively. Furthermore, ConA-Sepharose chromatography, native gel electrophoresis, and activity staining revealed that CMCase produced in yeast was glycosylated and its molecular weight was larger than that of the unglycosylated form from B. subtilis. Taking these findings together, SGA has the potential of secretion to culture medium, and the putative signal sequence of SGA can efficiently direct bacterial CMCase to the yeast secretion pathway.

• Journal of KIISE:Software and Applications
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• v.36 no.9
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• pp.732-748
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• 2009

The technology of Semantic Web realizes the base technology for context-awareness that creates new services by dynamically and flexibly combining various resources (people, concepts, etc). According to the realization of ubiquitous computing technology, many researchers are currently working for the embodiment of web service. However, most studies of them bring about the only predefined results those are limited to the initial description by service designer. In this paper, we propose a new service supporting model to provide an automatic method for plan related tasks which achieve goal state from initial state. The inputs on an planner are intial and goal descriptions which are mapped to the current situation and to the user request respectively. The idea of the method is to infer context from world model by DL-based ontology reasoning using OWL domain ontology. The context guide services to be loaded into planner. Then, the planner searches and plans at least one service to satisfy the goal state from initial state. This is STRIPS-style backward planner, and combine OWL-S services based on AI planning theory that enabling reduced search scope of huge web-service space. Also, when feasible service do not find using pattern matching, we give user alternative services through DL-based semantic searching. The experimental result demonstrates a new possibility for realizing dynamic service modeler, compared to OWLS-XPlan, which has been known as an effective application for service composition.

• Journal of Technology Innovation
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• v.23 no.3
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• pp.341-372
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• 2015

While creativity and innovation is the key to drive the creative economy in the South Korea, the development of analysis framework to evaluate the size and performance is limited. The present study suggests a framework and a method to assess economic impact of the creative economy using inter-industry analysis which employs input-output efficiencies populated by the Korean Bank and empirical data from the national informatization survey conducted by the National Information-society Agency(NIA). The results indicated that, as of 2013, despite of economy downturn, the creative innovation based on the information communication technology(ICT) had been significantly led the production, value-added, and employment inducement. The effect is predominant in the creative industry in an broad sense, that is, technology intensive manufacturing industry. Theoretical and policy implications are discussed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.5
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• pp.117-121
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• 2018

As the paradigm shifts in the era of accelerated technological development and technology convergence, the defense sector is pursuing national defense research and development through selection and concentration, as well as the expansion of investments to develop defense science and technology to the highest possible level. In addition to investing heavily in cutting-edge weapons and core technologies that are consistent with the future, the defense sector is also developing investment strategies within a limited budget. In the private sector, the PD system has been introduced in order to maximize the performance of R&D policy and to improve the professionalism and responsibility of planning & management. In accordance with this trend, in the field of defense, the PD system of defense technology with specialization is being promoted, in order to strengthen the task planning capacity and to promote the utilization of private technology. Considering the need for coherent technical support for the core technology developed by R&D, the improvement of open technology planning systems and expansion of inter-civil & technical linkage, the use of the PD system in the defense field is indispensable. In this study, we review similar cases abroad and at home to examine the introduction and management of the PD system for defense technology. This study is based on the current state of the defense technology PD system that is being pursued.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.3
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• pp.297-302
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• 2006

We investigated gonadal development and sex ratio of artificial seedlings of the oblong rockfish Sebastes oblongus, based on samplings for 370 days just after parturition. The primordial germ cells and genital ridge appeared separately under the mesentery in the yolk-sac stage larva (total length: 7.10-7.77 mm) just after parturition. The primordial germ cells and genital ridge integrated to form primordial gonad in 5-day-old larvae (7.12-9.68 mm), and then proliferation of somatic cell and germ cell occurred in the gonad, which was maintained undifferentiated until 45-days after parturition (18.6-20.4 mm). The ovarian differentiation began in the larva of 50-days old (dab) after parturition (dap) (20.0-24.5 mm). The somatic tissues elongated from the both opposite end-sites of undifferentiated gonad were consequently fused and formed a complete ovarian cavity at 60-days old dap (25.5-32.0 mm). In 80-days old dap (37.3-47.2 mm), meiosis of oogonia occurred to be chromatin nucleolus stage oocyte. The perinucleolus stage oocytes appeared at in 130-days old dap (68.0-86.0 mm), and previtellogenic stage oocytes appeared in 370-days old dap (101.0-116.0 mm). Only female was observed in the artificially produced oblong rockfish in the present study. This result revealed the effect of higher temperature on the sex determination of the oblong rockfish..

• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.176-182
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• 2006

Sensitive and specific monoclonal antibody (MAb) was produced from hybridoma (1H11-5) obtained by fusion of myeloma cell (V653) and spleen cell isolated from mouse immunized sulfamthazine (SMZ)-HG-KLH. Direct competitive ELISA was developed for rapid detection of SMZ in milk samples using MAb against SMZ with optimized conditions between MAb and SMZ-HG-HRP conjugate, and applicable conditions for analysis of milk samples were established. Detection range of immunoassay was 0.1 to 100 ppb. Recoveries from spiked raw milk and processed milk samples averaged 82.1-120.7 and 82.1-97.1%, respectively.

• Journal of Life Science
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• v.29 no.4
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• pp.470-477
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• 2019

White jelly fungus (Tremella fuciformis Berk) is effective for hypertension, diabetes, obesity, lung disease, beauty, and has been known as an elixir of life in ancient Chinese dynasty. In this study, we investigated the anti-obesity and diabetic effects of the fermented white jelly fungus extract by the bioconversion process. We fed an obesity-inducing mouse with 5% non-fermented Tremella fuciformis (TF), 2.5% fermented Tremella fuciformis (FTF), and 5% FTF containing High Fats Diet (HFD) and HFD for 8 weeks, respectively. The oral glucose tolerance test (OGTT) was performed analysis after 7 weeks of feeding and the dietary intake, food efficiency ratio, body weight, liver, epididymal fat weight, and serum insulin level were measured after 8 weeks of feeding. Also, HOMA-IR was analyzed. The concentrations of serum total cholesterol, triglycerides test was analyzed. The FTF compare with 5% TF and HFD confirmed that 5% FTF reduced body weight, tissue weight, triglycerides concentration, HOMA-IR, respectively. As a result, we confirmed that the fermented white jelly fungus has the anti-obesity effect. Finally, this study can be used a basic data for obesity treatment using fermented white jelly fungus.

• Journal of Industrial Convergence
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• v.17 no.2
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• pp.9-14
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• 2019

This study was carried out to investigate how the basic plan of the Saemangeum development project that has been developed by the government since 1991 has been changed and to present a new development strategy by analyzing the future value and tourism resource value of Saemangeum development. The research methodology is based on the in - depth analysis methodology of the time series research materials and press releases published by the Saemangeum Development Administration, which oversees the development of Saemangeum. The government announced a plan to significantly reduce agricultural land and increase the proportion of non-agricultural land to 70% in order to create a multifunctional complex. After that, the government revised the basic plan of Saemangeum in 2014 and changed the plan to tailor the investment demand in order to cultivate investment demand, and introduced the special economic cooperation system for global economic cooperation. Therefore, the purpose of this paper is to analyze the process of Saemangeum development to date and to evaluate the value of Saemangeum development as a tourism resource, centered on the Master Plan of the Saemangeum Development Project changed by the government. Based on the results of this analysis, I would like to present the vision of Saemangeum tourism development considering Saemangeum 's environment and characteristics of the surrounding scenery by referring to trends and similar cases of international tourism.

• Journal of Industrial Convergence
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• v.17 no.2
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• pp.29-34
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• 2019

The purpose of this study is to analyze the achievements of employment support by the government support specialization project. The data used in this study are based on the comparison of 5 - year employment support field and the operation results of the program until 2014-2018. The results of the study are as follows. First, the overall employment rate of the university has been continuously increased. Especially, the employment rate of the specialization department has been higher than the employment rate of the non - specialization department. Second, as a result of the analysis of the employment capacity strengthening index and the learning capacity strengthening index, it showed a steady increase in each year and contributed to the cultivation of customized talents required by the local society and the national industry. Third, as a result of analyzing the satisfaction of students who are business users, it was confirmed that the business reflecting the demands of the consumers was realized. Fourth, the continuous improvement of the business and the reflux have made the infrastructure of the employment support project more advanced and the system of supporting employment of the university systematically established. In conclusion, the result of the employment support project according to the specialization program showed excellent results and it is necessary to complement theses results when establishing related business plan in the future.

• Particle and aerosol research
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• v.16 no.4
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• pp.107-117
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• 2020

Based on the airborne measurement results over a coal fired power plant and steel work in Dangjin city, SO2 emission amounts of each site are estimated (top-down emission). Airborne measurements were carried out on May-June and October-November 2019. The estimated SO2 emission in 2019 for the power plant was 1502.1 kg/hr and that for the steel work was 2850.5 kg/hr, higher as much as a factor of 2.5 and 2.0, respectively, than the emission amounts provided by both facilities (bottom-up emission). The outcomes strongly illustrates that well designed airborne observations can serve a quantitative diagnostic tool for bottom-up emission estimates. Further research direction to improve the reliability of the top-down emission estimates is suggested.