The study researched germination of the plants and growth of Fungus according to concentration of aqueous extracts in order to provide basic data for developing natural agricultural resources by using Persicaria longiseta. The seed germination of Amaranthus spinosus was inhibited at 25% P. longiseta extract, while Agrostis stolonifera ssp. palustris was not affected at all concentrations tested. Especially, the seed germination rate and fresh weight of Trifolium incarnatum at 20% P. blumei extract were higher than those of control plot. The early growth of most receptor plant seedlings was promoted at 25% and 50% of P. blumei extracts, but the radicle growth of all receptor plants was significantly inhibited at > 25% of P. longiseta extract. The response of receptor plants to P. longiseta extract was different according to the plant species and the plant parts. The growth of plant pathogenic fungus in PDA medium showed an increasing inhibition tendency with increasing concentrations of P. longiseta extract. Especially, P. longiseta extract showed the greatest antimicrobial activity against Phytophthora infestans, Phythium graminicola, and Pythium venterpoolii. The content of total phenolic compound in P. longiseta was higher in leaves (1082.3 mg/L) but lower in roots (228.6 mg/L) and stems (207.8 mg/L), which is an allelopathic chemical. As these results are summarized, P. longiseta have competitive advantage because they release phenolic compounds with allelopathic effect and affect on germination, growth and fungi growth on underground flora compared to native plants and they have eligibility for natural herbicide and germicide.
This experiment was carried out to investigate the effect of different feed conditions on growth, cast production and conversion efficiency of organic matter to the earthworm. The experiment was tested on cow manure(CM) mixed with rice hulls (RH) or rice straw(RS). The mixture ratios were designed as one time, two times and three times of cow manure volume, respectively. The CM and RH mixtures resulted better on growth rate, reproductive efficiency and wormcast production than those for CM and RS mixtures. Especially on three times of RH mixture showed the highest growth characteristics compared to the other mixtures. Although both were between 20 and 34 on carbon and nitrogen ratio, the result inferred that the difference on the growth might have been caused by feeding conditions. On the mixtures of cow manure with rice hulls(CM+RH) was significantly higher on values on the conversion rate and conversion efficiency of organic matter to earthworm tissues than the mixtures of cow manure with rice straw(CM+RS). The most concerned point of the mixture of earthworm feed is that high contents of volatile solid and total carbon that increases in conversion efficiency of organic matter to earthworm tissues, and also causes the increase in growth rate and reproductive efficiency. The wormcast could be used as a valuable plant growth medium or soil conditioner for sustainable agriculture and it may be due to their high qualities of physico-chemical properties.
Joa, Jae-Ho;Moon, Doo-Kyung;Koh, Sang-Wook;Son, Daniel
Korean Journal of Organic Agriculture
/
v.22
no.4
/
pp.719-730
/
2014
This study was conducted to select proper soil amendments in order to reduce the occurrence of potato scab and maintaining soil health by applications of dolomitic lime, sulfur, potassium sulfate, shell meal fertilizer, silicate fertilizer, lime nitrogen and ammonium sulfate fertilizer in different pH levels of volcanic ash soil with continuous cultivation of potato. In potassium sulfate-applied plot with a low soil pH, the incidence rate and disease severity of scab were lowest at 84.4% and 28.4%, respectively. Those were lowest among the treatments. Value of potato scab control was 12.3% and marketable yield of potato was highest at 93.2%. In lime nitrogen-applied plot (60 kg/10a), the incidence rate was low at 38.3%, and control value was 23.8% and marketable yield of potato was high at 66.3%. In relatively higher pH soils, the incidence rate of scab was lowest at 38.3% in the lime nitrogen-applied plot (60 kg/10a). Value of potato scab control was 23.8%, which was four times higher than that in sulfur-applied plot. Marketable yield of potato was highest at 66.3% in the lime nitrogen-applied plot. In the lime nitrogen plot infected with potato scab pathogen such as S. acidiscabies and S. scabiei were remarkably lower than other soil amendments at 2.5, 5, and 10 g/L concentrations of lime nitrogen using Glucose Yeast Malt (GYM) medium. In conclusion, this study suggests that potassium sulfate application in low pH soil (less than pH 5) and lime nitrogen application in relatively higher pH soil (more than pH 6) before potato seeding might be helpful to reduce the occurrence of potato scab.
Park, Kwang-Lai;Suga, Yuko;Hong, Seung-Gil;Lee, Chorong;Ahn, Minsil;Kim, Seok-Cheol;Hashimoto, Tomoyoshi
Journal of the Korea Organic Resources Recycling Association
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v.24
no.4
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pp.77-83
/
2016
The objectives of this study was to investigate the difference between organic-farming and conventional-farming soils relatives to soil chemical properties and microbial flora. Fifteen soil sampling sites were chosen from the certified organic upland farm, considered with its location, crop and application of organic compost types. Soil chemical properties were analyzed by standard methods established by National Institute of Agricultural Sciences, Rural Development Administration. For the soil chemical properties, the values of pH were ranged from 4.5 to 7.3. The values of electrical conductivity (EC) in the sampling sites were below 2 dS/m of convention cultivation soil. For analyzing the microbial flora, the bacillus(16S rDNA) and cladothricosis(18S rDNA) were analyzed by using PCR-DGGE (Denaturing Gradient Gel Electrophoresis) in the soil of 15 sampling sites. Cluster analysis of biodiversity index was performed by using pattern of DGGE. DGGE patterns and clustering analysis of bacterial DNA from soil extracts revealed that the bacterial community was differentiated between less than 5 years and more than 5 years depending on the cultivation history. But there was no consistent tendency between cultivation history and regional trend in the case of molds. Therefore, it would be very effective to analyze bacterial clusters of organically cultivated soils in long - term cultivated soil for more than 5 years.
The study was initiated to compare crop productivity as affected by a long-term application with homemade liquid fertilizers in leading organic cucumber (Cucumis sativus L.) farms in Suncheon and Kimcheon provinces. A Suncheon farm have applied an EM (effective organism) liquid fertilizer for one year and fifteen years, designating as EM 1-year and EM 15-year plots, respectively, with 4-year and 5-year application of native microbes-liquid fertilizer in Kimcheon farm, designating as Micro 4-year and Micro 5-year plots, respectively. pH in the EM-liquid fertilizer was high to approximately 7.7, and EC in the Micro-liquid fertilizer was 0.1 dS/m higher than those of EM-liquid fertilizer, with similar macro-nutrient concentrations observed in the both liquid fertilizers. Soil EC was the highest to the 10.0 dS/m for the liquid fertilizer with EM 1-year and showed less than 1.5 dS/m for other liquid fertilizer plots. Micro-liquid fertilizer plots had soil OM contents less than 20 g/kg, which was approximately two times less than those of EM plots. Soil microbial properties were not significantly different among the liquid fertilizer plots. SPAD and PS II values were significantly increased by EM 15-year plots with high levels of soil OM and EC. Liquid fertilizer plot with EM 1-year had high concentrations of T-N, Ca, and Na in the cucumber crops but low concentrations of P and Mg, in particular for low K of 1.2% which was two times less than those of desired level for an optimum cucumber growth. The lowest fruit yield was observed for the liquid fertilizer plot with EM 1-year with the highest soil EC accumulated. Liquid fertilizer plot with EM 15-year produced the expanded volume of crop canopy and increased fruit yield. Therefore, long-term of continuous application with an organic liquid fertilizer would have sustainably improved soil stability and the crop productivity.
Kim, Woo Sik;Park, Jee Sung;Ahn, In;Park, Kyung Hoon;Kim, Ki Hong
Korean Journal of Medicinal Crop Science
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v.22
no.4
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pp.270-275
/
2014
This study was conducted to select and develop effect of eco-friendly organic materials for the eco-friendly prevention of Anthracnose occurred in the ginseng. Anthracnose on ginseng is occurred by Colletotrichum gloeosporioides and the crop damage is severe especially from July to August after rainy season. The test results showed that control effect by test products materials on the three years ginseng and four years ginseng field was lower in eco-friendly organic materials than that of chemical pesticide. However, the control effect of bordeaux mixture was higher with 71.3% and 73.8% levels than those of mineral matter, microbial agent, and developed plants extract mixtures (Eugenol, Curcumin, Wood vinegar, etc). On the other hand, three types of developed plants extract mixtures (3 types) showed control effect in a range of from 58.1% to 63.6% against Anthracnose which was higher as compared with plant extract alone and sodium silicate regardless of ages of ginseng. The results of this study would attribute in verifying the control effect of eco-friendly materials against Anthracnose for ginseng through investigating antimicrobial compounds contained in the plants body. Also, it would be used as control method against Anthracnose occurred in ginseng by judging the right control time through monitoring occurrence of disease.
Journal of the Korea Organic Resources Recycling Association
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v.10
no.2
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pp.82-91
/
2002
The study was executed to evaluate the agricultural utilization of phyllite as composting bulking agent in pig manure. The effects of the composted pig manure were verified by adopting the mixture of 10% phyllite and 20% saw dust(PSPC 10) and 20% phyllite and 10% saw dust(PSPC 20). The pig manure mixed with 30% saw dust was used as a control(PSC). However C/N ratio was very decreased because humus was rapidly progressed at PSPC 20 plot during the composting. Also, $NH_4{^+}-N$, $NO_3{^-}-N$, pH and EC were quickly changed from after one week and CEC was the highest as $92cmol_c/kg$. And Total-N was hardly changed during the composting but total carbon was rapidly declined as processing compost. Therefore, the phyllite has positively showed comparing with others in the street as compost bulking agent and go ahead, it is concerned that this material should have to research and investigating, constantly.
Seasonal occurrence and tne effects of two organic farming materials on mulberry sucker, Anomoneura mori were investigated on mulberry grown for fruit production in Buan, Jeonbuk, South Korea.. Overwintered adults of A. mori occurred from late March to mid-May with a peak in mid-April. Overwintered females began to lay eggs on winter buds from mid-April. Emerged nymphs appeared from early May and peaked in mid-May. Newly eclosed adults, appearing from early June, soon scattered and were not found in the mulberry field after mid-June. Therefore, the optimal control period for A. mori was mid-April for adults and early May for nymphs. Sophora extract and derris extract were effective organic farming materials for controllingl the nymphs of A. mori. It was found that derris extract sprayed 2-3 times at 5-days intervals in early May effected good control against A. mori nymphs.
Park, Kyung-Hun;Yoon, Hyunjoo;Han, Beom Seok;Lee, Je-Bong;Jeong, Mi Hye;Cho, Namjun;Om, Ae Son;Paik, Min-Kyoung
Korean Journal of Environmental Agriculture
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v.33
no.4
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pp.395-402
/
2014
BACKGROUND: Azadirachta indica Extract(AIE) containing azadirachtin as active ingredient have been used worldwide as environment-friendly organic material having pest control properties. However, the extracts prepared with different solvent and from different plant site is very diverse and have different toxicity. METHODS AND RESULTS: In this study, the four week repeated oral dose toxicity test of aqueous AIE in Sprague-Dawley rats was carried out to investigate the toxic effect of liver, main toxicity target organ of AIE. The male and female rats were divided into 4 groups, respectively; control(0 g/Kg bw), low-dose group(0.5 g/Kg bw), middle-dose(1.0 g/Kg bw) and high-dose group(2.0 g/Kg bw). As a results, relative liver weight increased with dose-dependent of AIE(p<0.05). Serum LDH in all AIE-treated groups were significantly lower than the control in male rats(p<0.05). However, serum GOT and GPT were significantly increased in all male AIE-treated groups in male rats(p<0.05) and, in particular, increase of serum GPT in dose-dependent manner raise the possibility of liver damage. Even through serum GLU was increased significantly in high-dose group in male rats compared to control, there were no significant differences of urinary GLU among all groups(p<0.05). In addition, histopathological examination of the liver did not reveal any lesions in all AIE-treated groups. CONCLUSION: In conclusion, 4 weeks of the repeated oral administration of AIE 2.0 g/Kg to rats has resulted no toxic response in liver. Therefore, AIE was no indicated to have any toxic effect in the SD rats, when it was orally administrated below the dosage 2.0 g/Kg/day for 4weeks.
Coming with the well-being era, consumer's demand for safe agricultural products is increasing. It is urgent to develop an environment-friendly pear production system. Accordingly, this study was conducted to develop an environment-friendly pear production system by using several environment-friendly agricultural materials which is known to be effective in agricultural production. In the effects of environment-friendly agricultural materials on the soil chemical characteristics in pear orchard, the content of total carbon, organic matter, Ca and Mg increased a little respectively compared with those of 2008, and 2nd treatment showed the highest EC and Mg content in the soil among treatments. The content of leaf N, P and Ca in 2009 decreased compared with those of 2008, while Mg content showed no difference between 2008 and 2009 years. Average pear weight in 2009 was 31g higher than that of 2008 (682g) and 2nd treatment showed the highest pear weight (738g) among treatments. The average sugar content was higher in 2009 (12.6$^{\circ}Bx$) compared with that of 2008 (12.2$^{\circ}Bx$) and the plot of 2nd treatment highest sugar content (12.6$^{\circ}Bx$) among treatments. There were no difference in hunter value of L among treatments, but hunter value of a showed higher 1.62 in 2009 than that of 2008 (3.73). The highest of gumminess and cohesiveness of fruits were obtained from 1st treatment and adhesiveness and chewiness of fruits were obtained from 3rd and 1st treatment respectively. Firmness of fruit increased a little in 2009 compared with that of 2008, while the highest firmness was obtained from 3rd treatment with 1.63kg/5mm$\oint$ among treatments. Phosphate content in the peel of 'Niitaka' pear of fruit skin in 2009 (0.97g/kg) showed 0.06g/kg more content than that of 2008 (0.91g/kg), while the highest content was obtained from 3rd treatment (1.15g/kg). Potassium content in the peel of 'Niitaka' pear in 2009 was 8.20g/kg, which is 0.06g/kg more content than that of 2008 (7.82g/kg) and the highest content was obtained from 1st treatment (8.34g/kg) among treatments. The highest nitrogen content in the flesh of 'Niitaka' pear was obtained from 3rd treatment (4.32mg/g), while it was the lowest in control plot (3.10mg/g). Phosphate content in the flesh of 'Niitaka' pear in 2009 (8.20g/kg) showed 0.06g/kg more content than that of 2008 (7.82g/kg), while the highest content was obtained from 1st treatment (8.34g/kg). There were no difference of the potassium content in the fruit peel of 'Niitaka' pear between years, but 1st treatment showed the highest content (11.81g/kg) among treatments, while the lowest was obtained from the control plot (10.83g/kg).
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