• Journal of Life Science
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• v.31 no.1
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• pp.28-36
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• 2021

Edwardsiella piscicida is a significant cause of hemorrhagic septicemia in fish and gastrointestinal infections in humans. Survival bacteria require specialized mechanisms to adapt to environmental fluctuations. Hence, to understand the mechanism through which E. piscicida senses and responds to environmental osmolarity changes, we determined the protein expression profile and physiological properties under various salinity conditions in this study. The OmpR protein is a part of the Env-ZOmpR two-component system that has been implicated in sensing salt stress in bacteria. However, the physiological role played by this protein in E. piscicida remains to be elucidated. Therefore, in this work, the function of the OmpR protein in response to salt stress was investigated. Phenotypic analysis revealed that, in the mutant, three of the biochemical phenotypes were different from the wild type, including, citrate utilization, hydrogen sulfide, and indole production. Introduction of the plasmid containing the entire ompR gene to the mutant strain returned it to its parental phenotype. The retarded growth rate also partially recovered. Furthermore, in our studies, OmpR was not found to be related to cell motility. Taken together, our results from the mutational analysis, the growth assay, MALDI-TOF MS, qRT-PCR, and the phenotype studies suggest that the OmpR of E. piscicida is implicated in osmoregulation, growth, expression of porins (ETAE_1826), virulence-related genes (EseC, EseD and EvpC), and certain genes of unknown function (ETAE_1540 and ETAE_2706).

• Food Science and Industry
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• v.54 no.1
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• pp.11-28
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• 2021

Air pollution caused by particulate matters (PM) has become a global issue. PM is known to threaten human health by causing respiratory and cardiovascular disease. PM can be introduced to human gastrointestinal track through food intake, causing inflammation and changes in gut microbiota. Even at low PM concentrations, prolonged exposure to PM can cause significant accumulation of PM in food products. The adsorption of PM onto food surfaces is expected to be strongly influenced by the properties of food surfaces, but few studies have been reported. This paper examines several important food surface properties that may affect the interactions between PM and food surfaces, including surface wettability, surface charge, and surface microstructure. Understanding the adsorption of PM onto food surfaces can provide useful guidance for classifying PM-sensitive foods and controlling food chains, including cultivation, processing, preservation, and cooking, to ensure food safety against PM.

• Journal of Digital Convergence
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• v.19 no.3
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• pp.379-385
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• 2021

Tattoo, which has been 5000 years' history is the symbol of the collective society. It was used as one of the methods not only to defense and camouflage but also to decorate themselves. In modern society, tattoo culture is either limited to ethnic minorities or be used as a decorative element of personal preference. Among them, the Chinese ethnic minority, the Dai, is still maintaining its tattoo culture, but gradually faded. Hence, the necessity of protecting the tattoo culture of the Dai is essential. The procedures and methods of this study were conducted by analyzing an antique book, explore residential areas, data research, and data collection through interviews. According to research, the tattoo culture of the Dai has been consistently passed down through traditional tattoo pictorial books, the memories of tattooists, and the stories of people who got tattoos. However, the Dai's traditional culture and customs are influenced by the China political reform between 1952 and 1956, confirming that the size of tattoo culture was reduced than before and evanesces. As a result, in order to inherit the ethnic minorities Dai's tattoo heritage, records of tattoo culture are required. Besides, it is considered that the records of tattoo culture and interpretations of tattoo culture by tattooists and the people who got a tattoo due to aging in the future should be studied.

• The Journal of Internal Korean Medicine
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• v.43 no.1
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• pp.1-21
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• 2022

Purpose: The standard tool for the pattern identification is used for identifying patterns in patients using a questionnaire. The purpose of this study is to reorganize the standard tool for the pattern identification of gastroesophageal reflux disease (GERD) developed in 2017 and to analyze the reliability and validity of the standard tool for pattern identification by applying it to GERD patients. Methods: To reorganize the standard tool for the pattern identification of GERD developed in the previous study, we searched the literature in the main databases, OASIS (Oriental Medicine Advanced Searching Integrated System) and CNKI (China National Knowledge Infrastructure). We added the search results to the data used in the previous study and went through the reorganizing courses, such as evaluating the validity of the translation, the Delphi technique, and a small survey. After reorganization, the patients who visited the Kyunghee University Korean Medicine Center for GERD symptoms were provided the questionnaire, including the reorganized standard tool for pattern identification. We analyzed the survey results to evaluate their reliability and validity. Results: Fifty patients completed the questionnaire. Reliability analysis results showed a pattern identification match rate of 86%, Cronbach's α coefficient of 0.834, and intraclass correlation coefficient of 0.907. The Mann - Whitney U test and logistic regression were implemented to check the relations between the survey questions and pattern identification results; the Pearson correlation, compared with other scales, showed a moderate score. Conclusion: We reorganized the standard tool for the pattern identification of GERD to be updated on current issues and so that it is easily used. The analysis results of the questionnaire showed that the reorganized standard tool had high reliability and moderate validity.

• Journal of the Korea Convergence Society
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• v.13 no.4
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• pp.573-582
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• 2022

In order to develop samultang with reduced side effects, modified samultang using omija was oral administered to rats with ovarian resection, and changes in evaluation indicators of functional efficacy in women's menopause were measured. In weight gain, relative weight of uterus and vagina, blood lipid-related indicators, and changes in blood estradiol, there was no statistically significant improvement effect in modified Samultang compared to the control group. However, the expression of estrogen receptor alpha and beta in intrauterine tissue tended to increase, and the expression of phosphorylated ERK, which is known to be involved in estrogen receptor signaling, showed a significant increase in activation in ERK and AKT. The expression amount of phosphorylation AKT was not significant, but showed an increasing tendency. Even though the test substance was administered in a relatively small dose, it is judged that the test substance modified Samultang has the ability to activate estrogen receptor. In the future, it is expected that it can be used as a useful natural mixture to show the efficacy of samultang with fewer gastrointestinal disorders.

• Korean Journal of Food Science and Technology
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• v.54 no.3
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• pp.288-296
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• 2022

This study aimed to verify the effect of Citrus unshiu peel water extract (CUP) on a mouse model of acute gastritis (AG) induced by HCl/ethanol. Several studies have found that CUP has anti-inflammatory effects. The AG model was induced by oral administration of 150 mM HCl/60% ethanol (550 µL) to all groups except the control group. Also, for drug treatment, sucralfate (10 mg/kg) and CUP (100 or 200 mg/kg) were orally administered for 90 minutes before induction. The effect of CUP treatment was confirmed by gross gastric mucosal damage measurement, and the levels of Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT), and myeloperoxidase were reduced as well as the levels of oxidative stress biomarkers and their related proteins. In addition, the levels of inflammatory proteins, mediators, and cytokines were significantly downregulated byPI3K/Akt signaling. Taken together, these results show that CUP treatment alleviates AG by regulating PI3K/Akt signaling.

• KIPS Transactions on Software and Data Engineering
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• v.10 no.12
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• pp.569-578
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• 2021

Thanks to web development today, we can easily access online news via various media. As much as it is easy to access online news, we often face fake news pretending to be true. As fake news items have become a global problem, fact-checking services are provided domestically, too. However, these are based on expert-based manual detection, and research to provide technologies that automate the detection of fake news is being actively conducted. As for the existing research, detection is made available based on contextual characteristics of an article and the comparison of a title and the main article. However, there is a limit to such an attempt making detection difficult when manipulation precision has become high. Therefore, this study suggests using a verifying article to decide whether a news item is genuine or not to be affected by article manipulation. Also, to improve the precision of fake news detection, the study added a process to summarize a subject article and a verifying article through the summarization model. In order to verify the suggested algorithm, this study conducted verification for summarization method of documents, verification for search method of verification articles, and verification for the precision of fake news detection in the finally suggested algorithm. The algorithm suggested in this study can be helpful to identify the truth of an article before it is applied to media sources and made available online via various media sources.

• Journal of Korea Entertainment Industry Association
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• v.15 no.4
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• pp.255-265
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• 2021

The purpose of this study was to examine the effects of Kahoot!(Game-based Student Response System) on nursing education. This study used an one group Pretest-posttest design. Participants were 179 nursing students from one D university located in Gwangju, Korea. The Kahoot!(Game-based Student Response System) was provided for 6 times. Data were collected between August 26 and October 25, 2019. The collected data was analyzed by descriptive statistics, paired t-test, pearson's correlation coefficient and stepwise multiple regression using IBM SPSS 21.0 program. The results showed that learning engagement(t=-6.257, p=.000) was significantly higher than levels before Kahoot!(Game-based Student Response System), critical thinking disposition(t=-2.163, p=.032) was significantly higher than levels before Kahoot!(Game-based Student Response System), problem solving ability(t=-3.032, p=.003) was significantly higher than levels before Kahoot!(Game-based Student Response System). Significant relationships were found among learning engagement(r=.375, p=.000), critical thinking disposition(r=.286, p=.000), problem solving ability(r=.291, p=.000) and learning satisfaction. The results of stepwise multiple regression indicates that learning engagement(β=.307, p=.000), problem solving ability(β=.158, p=.041) predicts 15.2% in learning satisfaction(F=16.905, p=.000). In conclusion, Kahoot!(Game-based Student Response System) is effective in improving learning engagement and problem solving ability to nursing education.

• Journal of Naturopathy
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• v.9 no.1
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• pp.13-21
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• 2020

Purpose: The purpose of this study examined the distribution of constitution(Con) types by examining the Chinese constitution table questionnaire with 594 Seoul citizens. It investigated the preferences of five colors by the Con. It then was to explore the validity of the test and the five colors according to the Con. Methods: We used the CC table questionnaire and surveyed by using a cardinal color. Results: The Con type distributions include yangweak(31.4%), yin-weak, gall-humidity, harmony, qi-weak, qi-gloom, specific, and extravasate in order. The lack of statistical significance also indicates that the CC test is not appropriate for Seoul citizens. The order of five preferences is 31% in blue, 25.3% in yellow, 18% in white, 17.7% in red, and 8% in black. Blue was the most preferred and black the least. There were differences in the distribution of five colors, but they were not statistically significant. In five viscera diseases of the yang-weak con, the stomach was more than half(50.3%). The highest preferred color was blue (30.9%). This difference was significant(p <.01). It evaluated that this influences the preference of five colors by five diseases. Conclusions: The constitution of Seoulian in the CC survey distributed into nine categories. There was a difference in preference of five colors by the con. However, it was not significant. This indicates that the Chinese con table is not suitable for Seoulian. However, the results are to be primary data for research in this field.

• Journal of Naturopathy
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• v.9 no.1
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• pp.1-12
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• 2020

Purposes: The purpose of this study was to investigate the relationship between five viscera, environmental and seasonal changes, and their preference for five colors. Methods: The survey performed using the Chinese medicine constitution and the five cardinal colors (Red, Blue, Yellow, White, Black) paper. Results: The blood types distributions were A, O, B, AB, and others in order. The proportion of smokers(75.6%) and non-drinkers(25.6%). Their color preferences were highest in blue and the lowest in black, but not significant. The proportion of the viscera was stomach(45.3%), liver-lung, intestine, and heart, and the tastes were spicy(48.8%), sweet, sour, salty, and bitter in order. Blue was the highest in all. kidneys(8.3%) preferred red and bitter yellow. The change in mind(35.7%) was highest, followed by scary thoughts and their color preferences varied. The most preferred place was 'cold place,' and 'dry place' was low. The most preferred seasons were 'autumn (38.6%), and the lowest was rainy. And their colors preference were blue. The respondents(89.9%) were normal, 5.7% hypertension and 4.4% hypotension. Healthy people preferred blue (91.8%) and hypertensives yellow with not significant. The diabetics preferred blue and red(p<.04). Conclusions: In the questionnaire survey on the health and physical abnormalities of Seoul citizens, various distributions found in each category, and most of them preferred blue. The results indicated that the questionnaire and the five- colored preference were a little interrelated. However, the results are to be primary data for research in this field.