• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.12-12
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• 2021

최근 길어진 여름 및 이상고온 현상이 지속됨에 따라 심화되는 광노화 피부의 특징으로는 건조, 굵고 깊은 주름, 탄력저하 및 불균일한 색소침착 등이 나타나게 됨. 화장품 소재는 기존 광노화 관련 화학물질인 Retinol 등을 대체하기 위해 자연 유래 성분을 적용한 신소재 연구를 진행하고 있음. 흰목이버섯(Tremella fuciformis)은 흰목이목에 속하는 버섯류로 자실체는 한천질로서, 주름이 되어 갈라져 있거나 또는 귓불 모양을 이루고 있으며, 크기는 10 cm 정도이다. 중국에서는 보양식의 주재료로 쓰일 만큼 탁월한 항노화 효능이 알려져 있다. 본 연구에서는 흰목이버섯의 자실체에서 추출하여 정제한 β-Glucan의 성분 확인, in vitro 수준의 피부 항노화 효과, 동물대체 독성 시험을 통한 피부독성 확인 및 인체 피부유효성 평가를 통한 항노화 효과를 확인하였다. 흰목이에서 추출, 정제 후 Bio-LC를 통한 유리당 분석 결과 Mannose, Fucose, Glucose를 확인하였으며, Human Keratinocyte에 UVB를 조사하여 광노화를 유발한 피부세포에 피부 자극 및 탄력저하 인자인 IL-6, TNFa 및 MMP-1을 평가한 결과 농도 의존적으로 현저히 개선됨을 확인하였다. 또한 보습 및 피부장벽 개선 인자인 Filaggrin과 Involucrin 생성효능을 평가한 결과 매우 높이 생성됨을 확인하였다. 본 연구결과를 토대로 광독성, 피부감작성 및 안점막 동물대체 독성시험을 실시한 결과 무독성임을 확인하여 피부에 안전하면서 효능이 우수한 것을 in vitro 수준에서 확인하였고, 피부 홍반완화, 주름개선, 탄력개선 및 보습증가 등 광노화 예방효과를 인체를 대상으로 평가한 결과 유의적인 홍반완화, 주름개선, 탄력 및 보습증가효과를 확인하였다. 본 연구결과를 종합하여 볼 때 흰목이버섯에서 추출, 정제한 β-Glucan은 in vitro 수준에서 자외선으로 인한 피부 트러블 완화, 탄력 및 보습개선을 확인하였고 독성시험을 통해 무자극임을 판정하였으며, 인체유효성 평가를 통해 광노화 예방효과를 확인하였으며 본 결과를 통해 아시아 및 글로벌 시장으로 천연유래 항노화 소재로 확장하고자 한다.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.3
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• pp.225-235
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• 2018

Phellinus gilvus is a medicinal mushroom used that has been used in folk medicine in Asian countries for centuries. The aim of this study was to investigate the anti-xanthine oxidase, anti-cholinesterase, and anti-inflammatory activities of methanol (ME) and hot water (HW) extracts prepared from fruiting bodies of Ph. gilvus. ME and HW had good anti-xanthine oxidase (XO) activities compared to allopurinol, an inhibitor of xanthine oxidase. ME showed comparable and slightly lower inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), respectively, than galanthamine, a standard AChE and BChE inhibitor. ME also showed a protective effect against glutamate-induced cytotoxicity at 40 mg/mL and 100 mg/mL in PC-12 cells. ME (0.5~2.0 mg/mL) significantly inhibited nitric oxide (NO) production in RAW 264.7 murine macrophage cells stimulated with lipopolysaccharide (LPS). Carrageenan-induced hind-paw edema in rats was significantly reduced 2~6 hr after treatment with 50 mg/kg of ME, which was comparable to administration of 5 mg/kg of indomethacin, the positive control. These results demonstrate that ME and HW of Ph. gilvus fruiting bodies possess good anti-xanthine oxidase, anti-cholinesterase, and anti-inflammatory activities.

• Environmental and Resource Economics Review
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• v.23 no.3
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• pp.365-408
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• 2014

The purpose of this paper is to compare efficiencies, two stage efficiencies, and the reduction of economic growth due to the restraint of $CO_2$ and fossil fuel in two efficiency approach by pointing out the limit of traditional efficiency approach and introducing material balance approach. The efficiencies under the traditional approach and the material balance approach report significant gaps when they are analyzed in detail. Especially, in case not including fossil fuels, the low income countries show the bad performance of production efficiencies, but OECD and high-middle income countries reveal the better performance than that of the low countries. It fails to reflect the reality justly. Based on the material balance approach, the low income countries report the higher performance and the alleviated effect of environmental components for economic growth than OECD's.

• KSBB Journal
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• v.18 no.3
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• pp.207-210
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• 2003

The locus coeruleus (LC) contains about half of the total number of noradrenergic neurons in the brain and those noradrenergic neurons from the LC innervate entire brain regions. The LC is a major common target region in several neurodegenerative disorders such as Alzheimer's, Pakinson's and Huntington's diseases. The brain-derived neurotrophic factor (BDNF) regulate neuronal cell survival and differentiation of central nervous system neurons, including LC noradrenergic neurons. In this study, various small molecules and growth factors were tested as candidates to promote the production of BDNF in LC noradrenergic neuronal cells. The molecules tested include neuropeptides, cytokines, growth factors, neurotransmitters, and intracellular signaling agents. Four small molecules or growth factors, FGF8b, BMP-4, forskolin, and dibutyryl cGMP, were found to increase the release of BDNF in LC noradrenergic neurons. Especially, BMP-4 significantly enhanced BDNF production over 2.5-fold in LC noradrenergic neurons.

• Journal of Convergence for Information Technology
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• v.8 no.6
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• pp.93-99
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• 2018

The purpose of this study was to prove the effect of passive stretching after delayed muscular pain induction in university students and to propose a rehabilitation exercise program for effective pain relief and prevention in case of injury. Subjects were divided into passive stretching group and control group. Passive stretching group was performed passive stretching after delayed muscular pain induction and control group did not perform any treatment after delayed muscular pain induction. The delayed muscular pain induction method was induced by bench step motion. The height of the step box was 50cm. The data were analyzed by two-way RGRM ANOVA for comparison of passive stretching group and control group. In conclusion, passive stretching after delayed myalgia has a positive effect on blood fatigue (CRP, LDH) and subjective pain scale(VAS).

• Korean Journal of Clinical Laboratory Science
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• v.56 no.1
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• pp.1-9
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• 2024

Exosomes are nano-sized membrane-bound extracellular vesicles containing various biological molecules, such as nucleic acids, proteins, and lipids, which can be used to modulate physiological processes. The exosomal molecules secreted by cells can be extensively used as tools for diagnosis and therapy. Exosomes carry specific molecules released by the cells they originate from, which can be transferred to surrounding cells or tissues by the exosome. For these reasons, exosomes can be exploited as biomarkers for diagnosis, carriers for drug delivery, as well as therapeutics. In stem cell technology, exosomes have been an attractive option because they can be used as safer therapeutic agents for stem cell-based cell-free therapy. Recently, studies have demonstrated the safety and efficacy of mesenchymal stem cell-derived exosomes in alleviating symptoms associated with coronavirus disease 2019 as they have anti-inflammatory and immunomodulatory potential. Performing multiple studies on exosomes would provide innovative next-generation options for clinical diagnostics and therapy. This review summarizes the use of exosomes focusing on their diverse roles. In addition, the potential of exosomes is illustrated with a focus on how exosomes can be exploited as powerful tools in the days to come.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2011.03a
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• pp.70-70
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• 2011

최근 산업 발달에 의한 환경문제와 석유자원 감소에 따라 섬유시장에는 Eco-friendly 바람이 강하게 불고 있으며 리싸이클 제품이 급부상하고 있다. 폴리에스터계 고분자 제품, 그 중에서도 PET 병의 재자원화를 살펴보면 기계적, 열적으로 처리하여 물질을 바꾸지 않고서 회수하는 방법인 Mechanical Recycling과 원료를 분해하여 회수하는 방법인 Chemical Recycling이 있다. 이러한 리싸이클을 통하여 에너지 절감, 저탄소, 유해물질 감소, 환경정화 및 폐기물 감소로 환경부하가 완화되며, 자원의 효율적 재사용이 가능하다. 리싸이클 PET 섬유제품은 의류 인테리어용과 같은 한정된 용도뿐만 아니라 자동차용 부품소재 및 내장 부품 등 산업용으로도 사용가능하다. 본 연구에서는 리싸이클 PET로 제직한 직물에 정련과 호발가공을 하여 이러한 전처리에 따른 물리적 열적 특성의 변화를 비교 분석하였다. 그 결과 정련과 호발가공을 한 리싸이클 PET 직물의 인장 인열 강도가 미처리 직물과 비교하여 감소하는 결과를 보였으며 마모 파열강도는 큰 차이를 보이지 않았다. 반면에 TGA와 DSC를 이용한 열분석 결과 열적 특성 및 결정화도는 일부 차이를 나타내었다.

• Environmental and Resource Economics Review
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• v.18 no.1
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• pp.105-138
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• 2009

This study investigates the spatial distribution of the degree of water pollutants in Han River using data obtained by the water pollution observation stations. This study estimates a non -parametric kernel density function for each water pollutants, and tests a significant difference between two estimated distribution functions. Next, Generalized Entropy inequality indices are evaluated and this research tests difference of inequality indices between two years using bootstrapping method. Lastly in a dynamic of view, it is analyzed that there are significant changes in the ranking of water pollution level. Estimation results show that the degree of inequality in spatial distribution of water pollution tends to be stable or decreasing for last 15 years in a great part of water pollutants, and ranking of water pollution level hardly changes in Han River.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.28 no.1
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• pp.186-201
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• 2002

Fructan(Levan)은 식물체 및 미생물에서 발견되는 탄수화물로 이는 과당(fructose)이 $\beta$-2, 6 결합으로 연결되어 있는 polysaccaride 이다. 본 연구에서는 Fructan을 생성하는 미생물(Zymomonas mobilis)과 10% sucrose(기질), 1-2% 효모 추출물을 주성분으로 하는 배지를 사용하여 30-37$^{\circ}C$, pH 5.0-7.0에서 20-24시간 동안 배양한후 원심분리하여 균체를 제거하고 3배량의 알코올을 가하여 침전, 건조하여 얻은 Fructan의 화장품 원료로서의 가능성을 조사하였다. 보습효과에 있어서는 Hyaluronic acid와 유사하였으며, keratinocyte에 대한 세포증식 효과를 나타내었다. 또한 3-D culture에 의해 구축된 생인공 피부내에 0.05%의 sodium lauryl sulfate (SLS)를 사용하여 피부자극에 의한 초기 염증 반응을 유도한후 0.01mg/m1, 0.05mg/m1의 Fructan을 각각 처리하였을 때, SLS만을 처리한 생인공피부와 비교하여 세포증식효능을 보였고, SLS 자극물질로 유도된 전염증성 조절인자인 interleukin-l$\alpha$(IL-l$\alpha$)의 분비량을 조사 하였을때 0.01mg/ml, 0.05mg/ml의 Fructan을 처리한 생인공피부의 IL-l$\alpha$ 양이 Fructan을 처리하지 않은 것보다 상대적으로 감소하였다. 이러한 결과로 Fructan이 생인공 피부내 피부 세포의 증식효과를 나타낼 뿐만 아니라, 또한 피부자극물질에 의한 염증반응에 대해 자극완화효능이 있음을 알 수 있었다. 섬유아세포 및 동물을 이용한 안전성 시험에서도 독성이 없는 안전한 원료로 평가되었다.

• Journal of Adhesion and Interface
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• v.13 no.1
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• pp.17-23
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• 2012

A fragrant microcapsule was prepared for use by students to reduce the stress of taking examinations. Rosmarinic acid was used as a fragrant oil which had the effect of relaxing stress, polycaprolactone (PCL) was used as a capsule wall material, and poly(vinyl alcohol) (PVA) as a stabilizer. The solvent evaporation method was used to form the microcapsule. The microcapsules were prepared by changing the stirring rate, the concentration of the stabilizer, and the molecular weight of PCL. The shape of the microcapsule was characterized by scanning electron microscopy (SEM). The size of the microcapsule was reduced by increasing the stirring speed. The release rate of rosmarinic acid was decreased when the higher molecular weight PCL was used. When the prepared microcapsule was tested in an aromatherapy class, the microencapsulated fragrant oil had a longer release time than the original fragrant oil. The study data showed that this fragrant oil was effective for increasing concentration ability, reducing stress, increasing digestive power, and increasing memory for the students.