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http://dx.doi.org/10.15324/kjcls.2018.50.3.225

Anti-Xanthine Oxidase, Anti-Cholinesterase, and Anti-Inflammatory Activities of Fruiting Bodies of Phellinus gilvus  

Yoon, Ki Nam (Department of Clinical Laboratory Science, Ansan University)
Jang, Hyung Seok (Department of Clinical Laboratory Science, Ansan University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.50, no.3, 2018 , pp. 225-235 More about this Journal
Abstract
Phellinus gilvus is a medicinal mushroom used that has been used in folk medicine in Asian countries for centuries. The aim of this study was to investigate the anti-xanthine oxidase, anti-cholinesterase, and anti-inflammatory activities of methanol (ME) and hot water (HW) extracts prepared from fruiting bodies of Ph. gilvus. ME and HW had good anti-xanthine oxidase (XO) activities compared to allopurinol, an inhibitor of xanthine oxidase. ME showed comparable and slightly lower inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), respectively, than galanthamine, a standard AChE and BChE inhibitor. ME also showed a protective effect against glutamate-induced cytotoxicity at 40 mg/mL and 100 mg/mL in PC-12 cells. ME (0.5~2.0 mg/mL) significantly inhibited nitric oxide (NO) production in RAW 264.7 murine macrophage cells stimulated with lipopolysaccharide (LPS). Carrageenan-induced hind-paw edema in rats was significantly reduced 2~6 hr after treatment with 50 mg/kg of ME, which was comparable to administration of 5 mg/kg of indomethacin, the positive control. These results demonstrate that ME and HW of Ph. gilvus fruiting bodies possess good anti-xanthine oxidase, anti-cholinesterase, and anti-inflammatory activities.
Keywords
Anti-inflammation; Anti-xanthine oxidase; Cholinesterase inhibitors; Phellinus gilvus;
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1 Wasser SP, Weis AL. Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: a modern perspective. Crit Rev Immunol. 1999;19:65-96.
2 Lindequist U, Niedermeyer THJ, Julich WD. The pharmacological potential of mushrooms. eCAM. 2005;2:285-299.
3 Yoon KN, Jang HS, Jin GH. Antioxidant, anti-diabetic, anti-cholinesterase, and nitric oxide inhibitory activities of fruiting bodies of Agaricus brasiliensis. Korean J Clin Lab Sci. 2015; 47:194-202.   DOI
4 Park WH, Lee HD. Illustrated book of Korean medicinal mushrooms. 2nd ed. Seoul: Kyohak Publishing; 2003. p359-360.
5 Bae JS, Jang KH, Yim H, Jin HK. Polysaccharide isolated from Phellinus gilvus inhibit melanoma growth in mouse. Caner Lett. 2005;218:43-52.   DOI
6 Yoon KN, Jang HS. Antioxidant and antimicrobial activities of fruiting bodies of Phellinus gilvus collected in Korea. Korean J Clin Lab Sci. 2016;48:355-364.   DOI
7 Shim SM, Im KH, Kim JW, Shim MJ, Lee MW, Lee TS. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Korean J Mycol. 2003;31:155-160.   DOI
8 Swain T, Hillis WE, Ortga M. Phenolic constituents of Prunus domestica. I. Quantitative analysis of phenolic constituents. J Sci Food Agric. 1959;10:83-88.
9 Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J Enthnopharmcol. 2000;71:109-114.   DOI
10 Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Immunol Meth. 1983;65:55-63.   DOI
11 Stirpe F, Corte Della E. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem. 1969;244:3855-3863.
12 Ellman GL, Courtney KD, Andres V Jr, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961;7:88-95.   DOI
13 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci. 1976;73:2424-2428.   DOI
14 Ma S, Liu H, Jiao H, Wang L, Chen L, Liang J, et al. Neuroprotective effect of ginkgolide K on glutamate-induced cytotoxicity in PC 12 cells via inhibition of ROS generation and $Ca^{2+}$ influx. Neurotoxicol. 2012;33:59-69.   DOI
15 Ryu JH, Ahn H, Kim JY, Kim YK. Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophage. Phytother Res. 2003;17:485-489.   DOI
16 Winter CA, Risley EA, Nuss GW. Carrageenan induced edema in the hind paw of rat as an assay for anti-inflammatory activity. Proc Soc Exp Biol Med. 1962;111:544-547.   DOI
17 Rocha-Guzmn NE, Gonzlez-Laredo JA, Conzalez-Laredo RF, Reynoso-Camacho R, Ramos-Gmez M, Garcia-Gasca T, et al. Antioxidant activity and genotoxic effect on HeLa cells of phenolic compounds from infusions of Quercus resinosa leaves. Food Chem. 2009;115:1320-1325.   DOI
18 Tayebati SK, Di Tullio MA, Amenta F. Effect of treatment with the cholinesterase inhibitor rivastigmine on vesicular acetylcholine transporter and choline acetyltransferase in rat brain. Clin Exp Hypertens. 2004;26:363-373.   DOI
19 Nguyen TK, Shin DB, Lee KR, Shin PG, Cheong JC, Yoo YB, et al. Antioxidant, anti-inflammatory and anti-acetylcholinesterase activities of fruiting bodies of Phellinus xeranticus. J Mushroom Sci Prod. 2013;11:278-286.   DOI
20 Yim HS, Chye FY, Ho SK, Ho WC. Phenolic profiles of selected edible wild mushrooms as affected by extraction solvent, time and temperature. Asian J Food Ag-Ind. 2009;2:392-401.
21 Ling X, Bochu W. A review of phytotherapy of gout: perspective of new pharmacological treatments. Pharmazie. 2014;69:243-256.
22 Yoon KN, Lee TS. In vitro antioxidant, anti-hyperglycemic, anti- cholinesterase, and inhibition of nitric oxide production activities of methanol and hot water extracts of Russula rosacea mushroom. J Mushrooms. 2015;13:1-10.   DOI
23 Choi DW. Excitotoxic cell death. J Neurologic. 1991;23: 1262-1276.
24 Naito M, Umegaki H, Iguchi A. Protective effects of probucol against glutamate-induced cytotoxicity in neuronal cell line PC 12. Neurosci Lett. 1995;186:211-213.   DOI
25 Choi WH, Oh YS, Ahn JY, Kim SR, Ha TY. Antioxidative and protective effects of Ulmus davidiana var. japonicus extracts on glutamate-induced cytotoxicity in PC 12 cells. Korean J Food Sci Technol. 2005;37:479-483.
26 Abbas AK, Lichtman AH, et al. Celluar and molecular immunology. 6th ed. Philadelphia: Saunders Elsevier; 2006. p75-77.
27 Nathan C. Nitric oxide as a secretory product of mammalian cell. The FASEB J. 1992;6:3051-3064.   DOI
28 Willeaume V, Kruys V, Mijatovic T, Huez G. Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences. J Inflamm. 1996;46:1-12.
29 Jang HJ, Kim AK, Pyo MY. Yang KS. Inhibitors ofnnitric oxide syntheasis from Phellinus pini in murine macrophages. J Pharm Soc Korea. 2007;51:430-434.
30 Fangkrathok N, Junlatat J, Sripanidkulchai B. In vivo and in vitro anti-inflammatory activity of Lentinus polychrous extract. J Ethnopharm. 2013;147:631-637.   DOI
31 Lee IG. Differential diagnosis and treatment of generalized edema. J Korean Acad Fam Med. 2003;24:6-10.
32 Storch I, Ferber E. Detergent-amplified chemiluminescence of lucigenin for determination of superoxide amino production by NADPH oxidase and xanthine oxidase. Anal Biochem. 1988; 169:262-267.   DOI
33 Costantino L, Albasini A, Rastelli G, Benvenuti S. Activity of polyphenolic crude extracts as scavengers of superoxide radicals and inhibitors of xanthine oxidase. Planta Med. 1992;58: 342-344.   DOI
34 Alam N, Yoon KN, Lee JS, Cho HJ, Lee TS. Consequence of the antioxidant activities and tyrosinase inhibitory effects of various extracts from the fruiting bodies of Pleurotus ferulae. Saudi J Biol Sci. 2012;19:111-118.   DOI
35 Cho YS, Kim ND, Kim SA. Effects of concurrent administration of aspirin and prednisolone on the anti-inflammatory and antipyretic activities in rats. J Pharm Soc Korea. 1978;22:128-137.
36 Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W, Lee L, Isakson P. Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci USA. 1994;91:12013-12017.   DOI
37 Beal MF. Mechanisms of excitotoxicity in neurologic diseases. PASEB J. 1992;6: 3338-3344.
38 Lim JH, Kim SH, Park NH, Moon CG, Kang, SS, Kim SH, Shin DH, Kim JC. Acute and chronic antiinflammatory effects of Phellinus linteus water extract in rats. J Biomed Res. 2010; 11:27-35.
39 Coyle JT, Price DL, DeLong MR. Alzheimer's disease: a disorder of cholinergeric innervation. Science. 1983;219:1184-1190.   DOI
40 Talesa VN. Acetylcholinesterase in Alzheimer's disease. Mech Aging Dev. 2001;122:1961-1969.   DOI
41 Block F, Pergande G, Schwarz M. Flupirtine reduces functional deficits and neuronal damage after global ischemia in rats. Brain Res. 1997;754:279-284.   DOI
42 Francis PT, Palmer AM, Snape M, Wilcock GK. The cholinergic hypothesis of alzheimer's disease: a review of progress. J Neurol Neurosurg Psychiatry. 1999;66:137-147.   DOI
43 Beal F. Aging, energy and oxidative stress in neurodegenerative diseases. Ann Neurol. 1995;38:357-366.   DOI
44 Colovic MB, Krstic DZ, Lazarevic-Pasti TD, Bondzic AM, Vasic VM. Acetylcholinesterase inhibitors: pharmacology and toxicology. Curr Neuropharmacol. 2013;11:315-335.   DOI
45 Xie QW, Whisnant R, Nathan C. Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon $\gamma$ and bacterial lipopolysaccharide. J Exper Medi. 1993;177:1779-1784.   DOI
46 Medzhitov R. Origin and physiological roles of inflammation. Nature. 2008:454:428-435.   DOI
47 MacMicking J, Xie QW, Nathan C. Nitric oxide and macrophage function. Annu Rev Immunol. 1997;15:323-350.   DOI
48 Hippeli S, Elstner EF. Inhibition of biochemical model reactions for inflammatory processes by plant extracts: a review on recent developments. Free Radi Res. 1999;31(Suppl):S81-S87.   DOI
49 Boumpas DT, Chrousos GP, Wilder RL, Cupps TR, Balow JE. Glucocorticoid therapy of immune-mediated diseases:basic and clinical correlates. Ann Intern Med. 1993;119:1198-1208.   DOI