• Title/Summary/Keyword: 오니

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Differential Expression Patterns of Gangliosides in the Liver and Heart of NIH-miniature Pigs (NIH-미니돼지의 간과 심장에서 갱글리오시드의 서로 다른 발현 패턴)

  • Ryu, Jae-Sung;Chang, Kyu-Tae;Kim, Ji-Su;Kwak, Dong-Hoon;Lee, Young-Choon;Oh, Keon-Bong;Choo, Young-Kug
    • Journal of Life Science
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    • v.20 no.4
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    • pp.467-473
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    • 2010
  • Gangliosides are a major component of the plasma membrane of mammalian cells, which are directly involved in a variety of immunological events, including cell-to cell or cell-to-protein interactions. In this study, we investigated whether gangliosides, sialic acid-containing glycosphingolipids, are related to rejection during the xenotransplantation of NIH-miniature pig livers and hearts to humans. Both high performance thin-layer chromatography and immunohistochemistry analyses revealed that the expression of gangliosides in the liver tissue of NIH-miniature pigs was higher than that in the heart. Gangliosides GD3, GD1a, GD1b, GT1b and GQ1b were observed in both the liver and heart, whereas GQ1b was detected only in the liver, indicating that the ganglioside expression profiles are tissue specific. Moreover, other ganglio-series gangliosides, including GM3, were not detected in the livers and hearts of NIH-miniature pigs. Taken together, these results suggest that gangliosides may play important roles in immune responses in clinical xenotransplants of pig livers and hearts.

Effect of Municipal Sewage Sludge Application on the Growth of Radish(Raphanus sativus L.) (무 생육(生育)에 대(對)한 도시하수오니(都市下水汚泥)의 시용효과(施用效果))

  • Kim, Bok-Jin;Hong, Seung-Beom;Kim, Tae-Ju
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.4
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    • pp.419-423
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    • 1996
  • Municipal sewage sludge produced at the Daegu Dalseo River Municipal Sewage Treatment Station was applied to radish(cv. Altari) to develop as an organic fertilizer. The sludge contained 53.59% of organic matter, 2.89% of nitrogen, and 3.5% of phosphate, so it seems to be enough to use as an organic fertilizer. Although the Cd and Pb contents of the sludge were lower than the official standard levels for the by-product fertilizer, it seems to be needed more researches due to the accumulation of the heavy metals both in soil and plants. Application of the sludge increased soil pH, organic matter, total nitrogen, available phosphate, exchangeable Ca and Mg contents, and CEC. Also application of the sludge tended to increase Cd and Pb contents slightly in soil. Application of the sludge increased N, $P_2O_5$, Cd, and Pb contents in plant and among the heavy metals Cd accumulated more than Pb. Yield of radish increased up to 1.000kg/10a of sludge in addition to the standard chemical fertilization or 3,000kg/10a of the sludge only.

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Mechanisms of the Formation of Geographic Misconceptions: A Case Study of High School Students' Misconceptions in the Subject of Korean Geography (지리 오개념 형성 메커니즘: 고등학생들의 한국지리 오개념을 사례로)

  • Kim, Minsung
    • Journal of the Korean Geographical Society
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    • v.49 no.4
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    • pp.601-614
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    • 2014
  • The purpose of this study is to investigate high school students' geographic misconceptions and their mechanisms of formation. Three main theories explaining why students develop misconceptions exist: 1) framework theory, 2) p-prim(phenomenological primitive) theory, and 3) categorization theory. This study chose three target geographic concepts, or, 1st and 2nd mountain ranges, secondary central business district and satellite city, and the Nopsae wind and the F$\ddot{o}$hn phenomenon. Then, this research explored students' typical misconceptions regarding these concepts and attempted to examine which theory explains the misconception forming processes most well. As a result, the following misconceptions were found. First, students understood that the numbers 1 and 2 denote the order of the formation of mountain ranges. Second, despite differences in their main functions, students tended to subsume the secondary central business district and satellite city under one functional category. Third, students believed that the Nopsae wind and the F$\ddot{o}$hn phenomenon are identical in hierarchy. This study explained students' creation of these misconceptions by applying the categorization theory in which students located a concept in an inappropriate location of an ontology tree.

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Microalgal Oil Recovery by Solvent Extraction from Nannochloropsis oceanica (Nannochloropsis oceanica로부터 용매추출법을 이용한 미세조류 오일 회수)

  • Park, Ji-Yeon;Lee, Gye-An;Kim, Keun-Yong;Kim, Ki-Yong;Choi, Sun-A;Jeong, Min-Ji;Oh, You-Kwan
    • Korean Chemical Engineering Research
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    • v.52 no.1
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    • pp.88-91
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    • 2014
  • In this study, oil as a source of biodiesel from Nannochloropsis oceanica was extracted using organic solvent. The oil extraction yield and efficiency from dry and wet microalgae were investigated. The initial fatty acids content of the N. oceanica was 317.8 mg/g cell showing a high oil content over 30%. The yield from dry microalgae was higher than that from wet microalgae due to the inhibition of water. The yield by chloroform-methanol was the highest and the yield by hexane was the lowest. However, the total fatty acids contents with the chloroform-methanol were 678.7 and 778.2 mg/g oil under dry and wet conditions, respectively. The high oil extraction yield by chloroform-methanol reflected the fact that the extracted oil contained a high level of impurity. The hexane-methanol extraction from dry N. oceanica showed high oil extraction efficiency, 82.6%. The chloroform-methanol extraction under wet condition also showed high efficiency, 88.0%. While the hexane-methanol extraction from dry microalgae is desirable under low drying cost, the chloroform-methanol extraction from wet microalgae is desirable under high drying cost.

Whitening Effect of Abelmoschus esculentus on Melanoma Cells (B16F10) (B16F10 세포에서의 오크라 추출물의 미백 활성 검증)

  • Yoo, Dan-Hee;Lee, In-Chul
    • Microbiology and Biotechnology Letters
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    • v.49 no.4
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    • pp.485-492
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    • 2021
  • In this study, the whitening effect of Abelmoschus esculentus extract was investigated to confirm its applicability in cosmetics. To determine the whitening effect, the tyrosinase-inhibitory activity of Abelmoschus esculentus hot water extract (AEWE) and 70% ethanol extract (AEEE) was measured. At the final concentration of 1000 ㎍/ml, AEWE showed an inhibitory activity of 22.2% and AEEE of 32.8%. To determine the whitening effect at the cellular level, the viability of melanoma cells treated with AEWE and AEEE was evaluated using the MTT assay. At concentrations of 100 ㎍/ml or less, both AEWE- and AEEE-treated groups showed cell survival rates of >95%. Furthermore, in both AEWE- and AEEE-treated melanoma cells, the melanin content decreased in a concentration-dependent manner. The inhibitory effects of AEWE and AEEE used at 5, 10, 50, and 100 ㎍/ml on protein expression were measured by western blot, with β-actin as the positive control. At a concentration of 100 ㎍/ml, AEWE showed an inhibitory effect of 88.1%, 24.8%, 62.2%, and 42.9% on microphthalmia-associated transcription factors (MITF), tyrosinase, tyrosinase-related proteins (TRP)-1, and TRP-2 factors, respectively. At the same concentration, AEEE showed inhibitory effect of 65.3%, 58.3%, 66.2%, and 65.3% against MITF, tyrosinase, TRP-1, and TRP-2 factors, respectively. In conclusion, the whitening effects of AEWE and AEEE were verified, and their applicability as a natural ingredient in cosmetics was confirmed.

Design and Implementation of JPetStore Order System Based Mobile WebApp Office (모바일 웹앱 오피스 기반의 JPetStore 주문시스템의 설계 및 구현)

  • Lee, Myeong-Ho;Han, Jung-Su
    • Journal of Digital Convergence
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    • v.10 no.3
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    • pp.149-154
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    • 2012
  • This paper aims to design and realize android smartphone mobile webapp office JPetStore order system based Seam framework. Currently in production until the lightweight container architecture, known commonly architecture is Seam framework. However, there is no design and implementation research of mobile webapp office with the Seam framework environment. In this study, the Seam environment based on the android mobile webapp office JPetStore order system implementation after analyzing the mobile webapp office development productivity with the objective is to provide guidance.

Development of a CAI Program on Atmospheric Pressure and the Effects of the CAI on Middle School Students' Science Achievement and Conceptual Change (대기압 개념학습을 위한 컴퓨터 보조수업 프로그램 개발 및 적용)

  • Kook, Dong-Sik;Choi, Dong-Ha
    • Journal of the Korean earth science society
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    • v.22 no.2
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    • pp.89-95
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    • 2001
  • The purposes of the study are (1) to develop a Computer-Assisted Instruction (CAI) program to deal with middle school students' misconceptions on the concept of atmospheric pressure, and (2) to investigate the effects of the CAI on students' achievement and conceptual change. The findings of the study are as follows: (1) Misconceptions about atmospheric pressure found are similar to the ones identified in earlier studies, (2) A CAI program on atmospheric pressure was developed based on the students' misconceptions identified, and (3) The instruction using CAI program has very positive effects on students' science achievement and conceptual change.

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Effects of Ethanol Extract from Leaves of Eleutherococcus senticosu on Hyperlipidemia in Rats (가시오갈피 잎 추출물이 흰쥐의 지질대사에 미치는 영향)

  • Park, Yu-Hwa;Kim, Hee-Yeon;Lim, Sang-Hyun;Kim, Kyung-Hee;Lee, Jeong-Hoon;Kim, Young-Guk;Ahn, Young-Sup
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.3
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    • pp.333-338
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    • 2012
  • The effects of ethanol extract from leaves of Eleutherococcus senticosu were evaluated on hyperlipidemic rats. Male SD rats were divided into a normal group, control (AIN-76 diet) group, Garcinia cambogia extract group, and AIN-76 diet group supplemented with ethanol extract from Eleutherococcus senticosu (EEES). The body weight of the AIN-76 group increased, whereas those of the Garcinia and EEES groups decreased. The serum total cholesterol of the AIN-76 group increased by 28.36% compared to the normal group, but decreased by only 27.15% in the Garcinia group and 25.47% in the EEES group. The serum triglyceride level of the AIN-76 group increased by 35.04% compared to the normal group, but decreased by only 26.76% in the Garcinia group and 37.54% in the EEES group. The serum HDL-cholesterol levels of the Garcinia and EEES groups increased compared to that of the AIN-76 group. The liver and epididymal adipose tissue weights of the EEES group decreased compared to those of the AIN-76 group. In measuring the concentration of triglycerides and total cholesterol level in the liver extracts, the AIN-76 group showed significant increases compared to the normal group, whereas the Garcinia and EEES groups showed a significant decrease compared to the AIN-76 group. These results indicate that the EEES group may improve lipid metabolism and reduce fat accumulation and body weight.