• Korean Journal of Plant Taxonomy
• /
• v.38 no.2
• /
• pp.151-162
• /
• 2008

Chromosome analysis using karyotyping and bicolor FISH were carried out in Pimpinella hallaisanensis which is one of the endemic plants in Jeju island of Korea. The somatic methaphase chromosomes number of this plant was 2n=2x=22 and the size of this chromosomes ranged from 3.58 to $5.82{\mu}m$. The chromosome complements consisted of two pairs of metacentrics (chromosomes 1 and 2), four pairs of submetacentrics (chromosomes 3, 4, 6 and 8) and five pairs of subtelocentrics (chromosomes 5, 7, 9, 10 and 11). Using bicolor FISH, three pairs of 5S and four pairs of 45S rDNA loci were observed. Two pairs of 5S rDNA signals were detected on the end of the long arm of chromosome 4 and one pair of them were observed between long arm end and centromere. Another 45S rDNA signals were detected on the end of short arm of chromosome 4, 6, 10 and 11, respectively. Hence, the chromosome number reexamined using both conventional staining and FISH methods was different from previous report.

• Journal of Animal Science and Technology
• /
• v.45 no.1
• /
• pp.1-12
• /
• 2003

The present study was carried out to establish the standard karyotype of the Korean Native Chicken and to find their chromosomal band markers using high-resolution banding technique. Chromosome analysis was performed on early chick embryos following in vitro culture of fertilized eggs of the yellow-brown and the red-brown lines of the Korean Native Chicken which had been established at National Livestock Research Institute. The high-resolution banding of the chromosome was achieved by treating the embryos with ethidium bromide and colchicine during culture. On GTG-banding, the Korean Native Chicken exhibited a typical chick banding pattern in all the macrochromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Chicken were virtually identical to those of White Leghorn and International System for Standardized Avian Karyotypes(ISSAK). However, the lengths and G-band numbers of the Korean Native Chicken macrochromosomes were greater than those of White Leghorn and ISSAK. Especially in chromosomes 1 and Z, the Korean Native Chicken exhibited more separated bands in compared with ISSAK. In C-banding patterns, although a lot of observed cells had C-band polymorphic patterns, almost the Korean Native Chicken macrochromosomes had heterochromatic C-band on centromeres and/or near terminal part. However, the heterochromatic C-band was constantly observed at the end of q-arm of Z chromosomes and on the whole W chromosome. In addition, the Korean Native Chicken exhibited distinctive heteromorphic patterns of C-bands on the centromere of chromosome 3 and at the end of q-arm of Z chromosome between homologous chromosomes.

• Korean Journal of Medicinal Crop Science
• /
• v.13 no.1
• /
• pp.48-51
• /
• 2005

Karyotype analysis and chromosomal locailization of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) were carried out in Jeffersonia dubia Benth., which is one of medicinal plants belonging to Berberidaceae. The somatic metaphase chromosome number was 2n=2x=12 and the size of chromosomes ranged $1.95{\sim}3.50\;{\mu}m$. The chromosome complement consisted of two pairs of metacentrics (chromosomes 1 and 3), two pairs of submetacentrics (chromosomes 2 and 4) and two pairs of subtelocentrics (chromosomes 5 and 6). In McFISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 2 and three pairs of 5S rDNA sites were detected on the short arm of chromosomes 4, 5 and 6, respectively.

• Journal of Plant Biotechnology
• /
• v.30 no.1
• /
• pp.7-11
• /
• 2003

Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using FISH (fluorescence in situ hybridization) technique were carried out in Nicotiana plumbaginifolia. Somatic chromosome number of N. plumbaginifolia was 2n=20 and kyarotype was composed of three pairs of metacentric chromosomes (1,2 and 7) and seven pairs of submetacentric chromosomes (3, 4, 5, 6, 8, 9 and 10). Length of chromosomes was ranged from 2.29 to 4.50 ${\mu}{\textrm}{m}$. Chromosome 1 and 2 were identified as satellite chromosomes. In FISH experiment, one pair of 5S rDNA signals was detected on the centromeric region of chromosome 2 and one pair of 45S rDNA signals was detected on the terminal region of chromosome 1.

• The Korean Journal of Malacology
• /
• v.14 no.1
• /
• pp.27-32
• /
• 1998

한국산 가시군부속 (Acanthochitona)의 좀털군부(A. achates),참털군부 (A. circellata), 털군부(A.defilippii)및 애기털군부(A. rubrolineata)등 4종의 정소를 재료로 warm drying mithod를 통해 염색체를 관찰하였다. 염색체의 수는 4종 모두 2n=16, n=8로 확인되었다. 핵형분석결과 형태적으로 좀털군부는 다섯 쌍의 중부염색체, 한쌍의 차중부염색체, 두 쌍의 차단부염색체로;참털군부는 한쌍의 중부염색체, 네 쌍의 차중부염색체, 두 쌍의 차단부염색체, 한 쌍의 말단부염색체로; 털군부는 세 쌍의 중부염색체, 세 쌍의 중부염색체, 한 쌍의 차중부염색체, 한 쌍의 차중부 또는 차단부염색체로 구성되어 있었다. 외부 형태적으로 매우 유사한 애기털군부와 좀털군부의 경우, 특히 4번 염색체에서 현저한 형태적 차이를 보였다. 8번 염색체의 경우, 애기털군부와 좀털군부는 중부염색체인 반면 참털군부와 털군부는 말단부염색체로 나타났다. 동북아시아와 유럽에 분포하는 가시군부류 사이에 나타나는 염색체의 속내 종간 숫적변이를 보면 동북아시아산(2n=16)이 유럽산(2n=16)이 유럽산(2n=24, 18)보다 분화된 것으로 보인다.

• Korean Journal of Ichthyology
• /
• v.1 no.1_2
• /
• pp.64-72
• /
• 1989

Karyological characteristics were investigated in 3 species of the genus Liobagrus from Korea. The diploid chromosome number in L. andersoni was found to be 28, with 9 pairs of metacentrics and 5 pairs of submetacentric chromosomes, and arm number (AN) was 56. L. mediadiposalis was found with 2n of 42, consisting of 13 pairs of metacentrics and 8 pairs of submetacentric chromosomes (AN=84). In the case of L. obesus 2n was 20, with 20 metacentric chromosomes (AN=40), which was the lowest among the species of the order Siluriformes. Sexual dimorphism or intraspecific polymorphism of the chromosomes was not observed in any species examined.

• Korean Journal of Plant Taxonomy
• /
• v.39 no.3
• /
• pp.193-198
• /
• 2009

Chromosome analysis using karyotyping and bicolor FISH were carried out for two Maackia species (M. fauriei and M. amurensis) found in Korea. The somatic metaphase chromosome number was 2n = 2x = 18 in both, and the size of these chromosomes ranged from 3.58 to $5.82{\mu}m$. The chromosome complements consisted of two pairs of metacentric (chromosomes 1 and 7), four pairs of submetacentrics (chromosomes 4, 6, 8 and 9) and three pairs of subtelocentrics (chromosomes 2, 3 and 5) in M. fauriei but, chromosomes 4 (subtelocentric) and 7 (submetacentric) of M. amurensis have different morphology. Using bicolor FISH, a pair of 45S rDNA loci were observed for both M. fauriei and M. amurensis, but the number and site of the 5S rDNA signal were different in the two species. M. fauriei has two pairs of 5S signals on chromosomes 7 and 8 but, M. amurensis has four paris on chromosomes 3, 4, 7 and 7. Hence, the 5S rDNA is a useful FISH for Maackia species.

• The Korean Journal of Nuclear Medicine
• /
• v.34 no.1
• /
• pp.74-81
• /
• 2000

Purpose: The purpose of this study was to develop in vivo dosimetries using both chromosomal aberrations and micronuclei in mice to assess biological effects of radiations. Materials and Methods: Five each mice were irradiated with 0, 1, 2, 3, 4, 5, 10 Gy of Cs-137 gamma-rays. We scored numbers of chromosomal aberrations in metaphase spreads and numbers of micronuclei in bone marrow smears under light microscope, and obtained the dose-response relationships. We also examined the relationship between the two dose-response curves. Results: The frequency of both chromosomal aberrations and micronuclei increased with dose, in a linear-quadratic manner The delta, beta, and alpha coefficients were 0.0176, 0.0324, and 0.0567 for metaphase analysis (r=1.0, p<0.001) and 0.0019, 0.0073, and 0.0506 for micronuclei assay (r=1.0, p<0.001). The frequency of chromosomal aberrations and micronuclei in different radiation doses was significantly correlated (r=0.99, p<0.01). Conclusion: In vivo dosimetry using either metaphase analysis or micronucleus assay was feasible in mice. These methods could be useful to evaluate biological effects of radiation.

• Animal Systematics, Evolution and Diversity
• /
• v.2 no.1
• /
• pp.1-10
• /
• 1986

문경산 흰 넓적다리 붉은 쥐, Apodemus peninsulae peninsulae, 의 염색체 핵형 분석과 단변량 및 다변량분석방법들을 사용한 등줄쥐, Apodemus agrarius coreae, 와의 형태적 형질의 비교를 하였다. 흰 넓적다리 붉은 쥐의 염색체수는 2n=50-54R지 수적 변이를 보였다. 차단부 염색체의 수는 48개로 개체간에 일정하였으나 , 중부 염색체의 수는 2-6개의 변이가 나타났다. 형태적 형질의 분석결과는 등줄쥐보다 흰 넓적다리 붉은 쥐가 크다고 판정되었으며 가장 구별이 잘 되는 경구개의 길이였다.

• The Korean Journal of Zoology
• /
• v.32 no.4
• /
• pp.358-364
• /
• 1989

한국산 박새속 조류 Porus major(박새), Porus atter(진박새), Porus poiustris(쇠박새), Parus varius(곤줄박이)의 핵형을 일반염색 방법으로 분석한 결과 4종의 염색체 수는 모두 2n=78∼80으로 나타났고, 성 염색체를 포함한 7쌍이 macrochromosome, 그 외 32∼33쌍이 microchromosome이었다. 종간 차이를 나타내는 염색체는 5번째 염색체와성염색체인 Z·W-염색체였다. 이러한 핵형의 차이는 5번째 염색체에서는 pericentric inversion, 성 염색체에서는 전좌에 의한 것으로 생각된다. The chromosomal analysis of Pows major, Paws after, Paws palustris and Paws vorius of the genus Paws in Korea were performed by conventional Giemsa staining method. The diploid number of four species were 2n=78-80, and there were 7 pairs of macrochromosomes and 32 or 33 pairs of microchromosomes. The 5th and Z·W-chromosomeswere distinctly different between interspecies. Probably these karyological differences were speculated by pericentric inversion in 5th chromosome and translocation in Z·W-chromosomes.