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http://dx.doi.org/10.5010/JPB.2003.30.1.007

Karyotype Analysis and Physical Mapping of rDNAs in Diploid Nicotiana plumbaginifolia  

Cho, Hye-Gyoung (Department of Biology, Chungnam National University)
Koo, Dal-Hoe (Department of Biology, Chungnam National University)
Kim, Soo-Young (Department of Biology, Chungnam National University)
Bang, Jae-Wook (Department of Biology, Chungnam National University)
Publication Information
Journal of Plant Biotechnology / v.30, no.1, 2003 , pp. 7-11 More about this Journal
Abstract
Karyotype analysis and chromosomal localization of 5S and 45S rDNAs using FISH (fluorescence in situ hybridization) technique were carried out in Nicotiana plumbaginifolia. Somatic chromosome number of N. plumbaginifolia was 2n=20 and kyarotype was composed of three pairs of metacentric chromosomes (1,2 and 7) and seven pairs of submetacentric chromosomes (3, 4, 5, 6, 8, 9 and 10). Length of chromosomes was ranged from 2.29 to 4.50 ${\mu}{\textrm}{m}$. Chromosome 1 and 2 were identified as satellite chromosomes. In FISH experiment, one pair of 5S rDNA signals was detected on the centromeric region of chromosome 2 and one pair of 45S rDNA signals was detected on the terminal region of chromosome 1.
Keywords
5S rDNA; 45S rDNA; FISH; karyotype; satellite chromosome;
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1 Fransz PF, Amstrong S, de Jong JH, Parnell LD, van Drunen C, Zabel P, Bissing T, Jones GH (2000) Integrated cytogentic map of chromosomearm 4S of A. thaliana: structural organization of heterochromatic knoband centromere region. Cell 100: 367-376   DOI   ScienceOn
2 Fukui K, Ohmido N, Khush GS (1994) Variability in rDNA loci in the genus Oryza detected through fluorescence in situ hybridization. Theor Appl Genet 87: 893-899   DOI   ScienceOn
3 Heslop-Harrison JS, Schwarzacher T (1993) Molecular cytogenetics-biology and applications in plant breeding. Chromosomes Today 11: 191-198
4 Jamet E, Durr A, Fleck J (1987) Absence of some truncated genes in the amphidiploid Nicotiana tabacum. Gene 59: 213-21   DOI   ScienceOn
5 Jiang J, Gill BS (1994) New 18. 26S ribosomal RNA gene loci: chromosomal landmarks for the evolution of polyploid wheats. Chromosoma 103: 179-185   DOI
6 EI-Gadi A, Elkington TT (1975) Comparision of the Giemsa C-band karyotype and the relationshops of Allium cepa, A. fistulosum and A. glantum. Chromosoma 51: 19-23   DOI
7 Flavell RB, O'Dell M, Thompson WF, Vincentz M, Sardana R, Barker RF (1986) The differential expression of ribosomal RNA genes. Philops Trans R Soc London, Ser B 314: 379-385
8 Kovtun YV, Komarnitsky IK, Gleba YY (1993) A new middle repetitive sequence of Nicotiana plumbaginifolia genome. Plant Mol BioI 23: 435-438   DOI   ScienceOn
9 Leitch lJ, Heslop-Harrison JS (1993) Physical mapping of four sites of rDNA sequences and one site of the $\alpha$-amylase-2 gene in barley (Hordeum vulgare). Genome 36: 517-523   DOI   ScienceOn
10 Leitch IJ, Leitch AR, Heslop-Harrison JS (1994) Physical mapping of plantDNA sequences by simultaneous in situ hybridization of two differently labelled fluorescent probes. Genome 34: 329-333
11 Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hireditas 52: 201-220   DOI
12 Maluszynska J, Heslop-Harrison JS (1993a) Molecular cytogenetics of the genus Arabidopsis: In situ hybridization of rDNA sites, chromosome numbers and diversity in centrometric heterochro-momatin. AnnBota 71: 479-484   DOI   ScienceOn
13 Maluszynska J, Heslop-Harrison JS (1993b) Physical mapping of rDNA loci in Brassica species. Genome 36: 774-781   DOI   ScienceOn
14 Chen CM, Chen CC, Wang CT, Ho CH, Keo YY (1997) Two tandemly repeated telomere-associated sequences in Nicotiana plumbaginifolia. Chromosome Res 5: 561-568   DOI
15 Schneeberger RG, Creissen GP, Cullis CA(1989) Chromosomal and molecular analysis of 5S gene organization in flax, Linum usitatissimum. Gene 83: 75-84   DOI   ScienceOn
16 Yamamoto M, Mukai Y (1989) Application of fluorescence in situ hybridization to molecular cytogenetics of wheat. Wheat lnf Serv 69: 30-33
17 Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot 64: 315-324
18 Schwarzacher T, Leitch AR, Heslop-Harrison JS (1990) In situ hybridization and the architecture of the nucleus. Trans R Microscop Soc 1:669-674
19 Villa A (1984) The chromosome idiogram of Nicotiana plumbagini-folia. Genetica (Thehague) 64: 145-148   DOI
20 Kenton A, Khashoggi A, Parokonny A, Bennett MD ichtenstein C (1995)Chromosomal location endogenous geminivires-related DNA sequences in Nicotiana tabacum L. Chromosome Res 3: 346-350   DOI
21 Kim SY, Lim YP, Bang JW (1998) Cytogenetic analysis of Brassica campestris var. pekinensis using C-Banding and FISH. Korean J Genetics 20: 285-294
22 Koo DH, Hur YK, Jin DH, Bang JW (2002) Karyotype analysis of a Korean cucumber cultivar (Cucumis sativusL. cv. Winter Long) using C-banding and Bicolor Fluorescence in situ Hybridization. Mol Cells 13:413-418
23 Murata M, Heslop-Harrison JS, Motoyoshi F (1997) Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones. Plant J 12:31-37   DOI   ScienceOn
24 Moore G, CheungW, FooteT, GalesM, Koebner R, Leitch AR, Leitch lJ, Money T, Stacombe P, Vano M, Flavell R (1993) Key features of cereal genome organization as revealed by the use of cytosine methylation-sensitive restriction endonuleases. Genomics 15: 472-482   DOI   ScienceOn
25 Mukai Y, Endo T, Gill BS (1990) Physical mapping of the 5S rRNA multigene family in common wheat. J Hered 81: 290-295