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http://dx.doi.org/10.5187/JAST.2003.45.1.001

Identification of Chromosomal Band Markers of the Korean Native Chicken  

Baik, K. H. (Regional Animal Industry Research Center, Jinju National University)
Lee, C. Y. (Regional Animal Industry Research Center, Jinju National University)
Sang, B. D. (National Livestock Research Institute, R. D. A.)
Choi, C. H. (National Livestock Research Institute, R. D. A.)
Kim, H. K. (National Livestock Research Institute, R. D. A.)
Sohn, S. H. (Regional Animal Industry Research Center, Jinju National University)
Publication Information
Journal of Animal Science and Technology / v.45, no.1, 2003 , pp. 1-12 More about this Journal
Abstract
The present study was carried out to establish the standard karyotype of the Korean Native Chicken and to find their chromosomal band markers using high-resolution banding technique. Chromosome analysis was performed on early chick embryos following in vitro culture of fertilized eggs of the yellow-brown and the red-brown lines of the Korean Native Chicken which had been established at National Livestock Research Institute. The high-resolution banding of the chromosome was achieved by treating the embryos with ethidium bromide and colchicine during culture. On GTG-banding, the Korean Native Chicken exhibited a typical chick banding pattern in all the macrochromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Chicken were virtually identical to those of White Leghorn and International System for Standardized Avian Karyotypes(ISSAK). However, the lengths and G-band numbers of the Korean Native Chicken macrochromosomes were greater than those of White Leghorn and ISSAK. Especially in chromosomes 1 and Z, the Korean Native Chicken exhibited more separated bands in compared with ISSAK. In C-banding patterns, although a lot of observed cells had C-band polymorphic patterns, almost the Korean Native Chicken macrochromosomes had heterochromatic C-band on centromeres and/or near terminal part. However, the heterochromatic C-band was constantly observed at the end of q-arm of Z chromosomes and on the whole W chromosome. In addition, the Korean Native Chicken exhibited distinctive heteromorphic patterns of C-bands on the centromere of chromosome 3 and at the end of q-arm of Z chromosome between homologous chromosomes.
Keywords
Korean native chicken; G-banding; C-banding; High-resolution banding; Chromosomes;
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