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Karyotyping Analysis and Bicolor FISH of Pimpinella hallaisanensis, an Endemic to Jeju Island  

Kim, Soo-Young (Korea Research Institute of Bioscience and Biotechnology)
Kim, Chan-Soo (Warm-Temperate Forest Research Center, Korea Forest Research Institute)
Tho, Jae-Hwa (Warm-Temperate Forest Research Center, Korea Forest Research Institute)
Lee, Joongku (Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Plant Taxonomy / v.38, no.2, 2008 , pp. 151-162 More about this Journal
Abstract
Chromosome analysis using karyotyping and bicolor FISH were carried out in Pimpinella hallaisanensis which is one of the endemic plants in Jeju island of Korea. The somatic methaphase chromosomes number of this plant was 2n=2x=22 and the size of this chromosomes ranged from 3.58 to $5.82{\mu}m$. The chromosome complements consisted of two pairs of metacentrics (chromosomes 1 and 2), four pairs of submetacentrics (chromosomes 3, 4, 6 and 8) and five pairs of subtelocentrics (chromosomes 5, 7, 9, 10 and 11). Using bicolor FISH, three pairs of 5S and four pairs of 45S rDNA loci were observed. Two pairs of 5S rDNA signals were detected on the end of the long arm of chromosome 4 and one pair of them were observed between long arm end and centromere. Another 45S rDNA signals were detected on the end of short arm of chromosome 4, 6, 10 and 11, respectively. Hence, the chromosome number reexamined using both conventional staining and FISH methods was different from previous report.
Keywords
bicolor FISH; chromosome number; karyotyping; Pimpinella hallaisanensis; rDNAs;
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Times Cited By KSCI : 6  (Citation Analysis)
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