Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Karyotyping Analysis and Bicolor FISH of Pimpinella hallaisanensis, an Endemic to Jeju Island

제주특산 한라참나물(Pimpinella hallaisanensis)의 핵형분석과 Bicolor FISH

  • Kim, Soo-Young (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chan-Soo (Warm-Temperate Forest Research Center, Korea Forest Research Institute) ;
  • Tho, Jae-Hwa (Warm-Temperate Forest Research Center, Korea Forest Research Institute) ;
  • Lee, Joongku (Korea Research Institute of Bioscience and Biotechnology)
  • 김수영 (한국생명공학연구원) ;
  • 김찬수 (국립산림과학원 난대산림연구소) ;
  • 도재화 (국립산림과학원 난대산림연구소) ;
  • 이중구 (한국생명공학연구원)
  • Received : 2008.02.01
  • Accepted : 2008.03.24
  • Published : 2008.06.30
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Abstract

Chromosome analysis using karyotyping and bicolor FISH were carried out in Pimpinella hallaisanensis which is one of the endemic plants in Jeju island of Korea. The somatic methaphase chromosomes number of this plant was 2n=2x=22 and the size of this chromosomes ranged from 3.58 to $5.82{\mu}m$. The chromosome complements consisted of two pairs of metacentrics (chromosomes 1 and 2), four pairs of submetacentrics (chromosomes 3, 4, 6 and 8) and five pairs of subtelocentrics (chromosomes 5, 7, 9, 10 and 11). Using bicolor FISH, three pairs of 5S and four pairs of 45S rDNA loci were observed. Two pairs of 5S rDNA signals were detected on the end of the long arm of chromosome 4 and one pair of them were observed between long arm end and centromere. Another 45S rDNA signals were detected on the end of short arm of chromosome 4, 6, 10 and 11, respectively. Hence, the chromosome number reexamined using both conventional staining and FISH methods was different from previous report.

제주도 특산식물인 한라참나물의 염색체수를 재조사하기 위해 핵형분석과 bicolor FISH를 수행하였다. 한라참나물의 체세포 염색체수는 2n=2x=22로 관찰되었고, 염색체의 길이는 $3.58-5.82{\mu}m$였다. 염색체의 구성은 2쌍의 중부 염색체(염색체 1과 2번), 4쌍의 차중부 염색체 (염색체 3, 4, 6, 8번), 그리고 5쌍의 차단부 염색체(염색체 5, 7, 9, 10, 11번)로 확인되었다. Bicolor FISH를 통해 3쌍의 5S와 4쌍의 45S rDNA 위치를 확인하였으며, 5S rDNA의 경우 염색체 4번의 장완 말단 부위에서 2쌍과 염색체 6번의 장완 중심과 동원체 사이에서 1쌍의 signals가 관찰되었다. 45S rDNA signals는 염색체 4, 6, 10 그리고 11번의 단완 말단에서 각각 확인되었다. 본 종의 염색체수가 핵형분석 및 FISH를 통해 이전의 연구결과와 다르게 분석됨으로써 한라참나물의 염색체수의 재고가 필요하다고 사료되었다.

Keywords

References

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