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Karyotype Analysis and Physical Mapping of rDNAs Using McFISH in Jeffersonia dubia Benth  

Kim, Soo-Young (School of Bioscience & Biotechnology, Chungnam Natl. Univ.)
Choi, Hae-Woon (School of Bioscience & Biotechnology, Chungnam Natl. Univ.)
Koo, Dal-Hoe (School of Bioscience & Biotechnology, Chungnam Natl. Univ.)
Kim, Chan-Soo (Warm-Temperate Forest Research Center, Korea Forest Research Institute)
Bang, Jae-Wook (School of Bioscience & Biotechnology, Chungnam Natl. Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.13, no.1, 2005 , pp. 48-51 More about this Journal
Abstract
Karyotype analysis and chromosomal locailization of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) were carried out in Jeffersonia dubia Benth., which is one of medicinal plants belonging to Berberidaceae. The somatic metaphase chromosome number was 2n=2x=12 and the size of chromosomes ranged $1.95{\sim}3.50\;{\mu}m$. The chromosome complement consisted of two pairs of metacentrics (chromosomes 1 and 3), two pairs of submetacentrics (chromosomes 2 and 4) and two pairs of subtelocentrics (chromosomes 5 and 6). In McFISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 2 and three pairs of 5S rDNA sites were detected on the short arm of chromosomes 4, 5 and 6, respectively.
Keywords
Jeffersonia dubia; karyotype; rDNAs; McFISH;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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