• Title/Summary/Keyword: 연구 분석

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Anti-inflammatory effect and useful contents of saccharification extract powder using hot water extract from log cultivation Lentinula edodes by different UV irradiation (UV 조사시간에 따른 원목표고당화물의 유용성분 및 항염증 효과)

  • Yun, Kyeong-Won;Im, Seung-Bin;Jin, Seong-Woo;Kim, Kyung-Je;Koh, Young-Woo;Ha, Neul-I;Jeong, Hee-Gyeong;Jeong, Sang-Wook;Kim, Seung-Ju;Kim, Bok-Seon;Kim, Ki-Man;Choi, Yu-Jin;Song, Da-Hye;Seo, Kyoung-Sun
    • Journal of Mushroom
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    • v.18 no.4
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    • pp.357-364
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    • 2020
  • The grade and price of Lentinula edodes largely differs in preference depending on the product area and seasonal factors. The product amount of autumn L. edodes was higher than that of spring L. edodes, but high quality, which is divided into "Hwago" is low in preference. Mostly, the autumn L. edodes is obtained as powder; hence, it is necessary to develop a processing method to utilize its flavor and aroma at an affordable price. Additionally, we estimated the content of 𝛽-glucan, ergosterol, vitamin D2, reducing sugars, and free amino acids and evaluated the anti-inflammatory activity of saccharification powder of log-cultivated L. edodes. In the saccharification powders obtained via 7 min of UV irradiation of log-cultivated L. edodes, 𝛽-glucan and vitamin D2 contents were found to be the highest, whereas ergosterol content was found to be the lowest. The content of reducing sugars ranged from 62.4 mg/L to 68.2 mg/L. The free amino acids were higher in these saccharification powders than in the control. Subsequently, RAW 264.7 cells were treated with different concentrations (10, 50, 100, 200, 300, and 500 ㎍/mL) of the saccharification powders of log-cultivated L. edodes obtained via different UV irradiation time applications. The cells showed good viability; the anti-inflammatory effect was found to be the highest at 7 min UV irradiation. Therefore, 7 min of UV irradiation was determined to be the optimum condition for manufacturing saccharification powders of log-cultivated L. edodes. Hence, saccharification powders of log-cultivated L. edodes may be used as a raw material for natural sweeteners, food additives, and in the food industry.

Comparative study of Seed Productivity of Spring Sown Italian Ryegrass(Lolium multiflorum Lam.) Depending on Seeding Distance in Gangwon Highland (강원 고지대에서 봄 파종 이탈리안 라이그라스(Lolium multiflorum Lam.)의 파종 간격에 따른 종자 생산성 비교 연구)

  • Jeong, Eun Chan;Li, Yan Fen;Kim, Hak Jin;Kim, Meing Joong;Ji, Hee Chung;Kim, Jong Geun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.41 no.1
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    • pp.16-22
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    • 2021
  • This experiment was conducted to study on the growth characteristics and seed productivity of Italian ryegrass (Lolium multiflorum Lam., IRG) planted in the Spring in Gangwon Highland according to the seeding distance (20, 30 and 40 cm). The field was located in highland around 600 m above sea level. The experimental design was randomized block design with three replication and the tested IRG variety was 'Greencall' developed by National Institute of Animal Science (NIAS). IRG was sown on March 26, 2020, and harvested on July 2. The plant height was the shortest at 80.5 cm in the 40 cm seeding distance plot (P<0.05), and there was no significant difference between the 20 and 30 cm seeding distance. The number of spike per square meter (㎡) was significantly higher in the 20 cm seeding distance plot than that of 40 cm (937 vs. 571). The dry matter (DM) content of seed and straw after harvesting was 49.70 and 33.36 % on average, and there was no significant difference between treatments (P>005). However, there was a significant difference in the fresh and DM yield of seeds and straw (P<0.05). DM yield of seeds was significantly higher in 20 cm distance than that of 40 cm, and the yield of straw was the same trend. On the other hand, there was no significant difference in DM yield between 20 cm and 30 cm and also in the feed value of straw after seed harvesting among seeding distance. The average CP, ADF, NDF, and TDN contents were 6.91, 36.76, 61.75 and 59.86%, respectively, and the RFV value was 91. Considering the above results, the production of Italian ryegrass seeds sown in the spring in the highlands of the Gangwon is lower than that of autumn sowing, but it is judged that it needs to be reviewed in case it is unavoidable. In the future, there should be an economic analysis and the development of technology that can increase production.

Validation of Surface Reflectance Product of KOMPSAT-3A Image Data: Application of RadCalNet Baotou (BTCN) Data (다목적실용위성 3A 영상 자료의 지표 반사도 성과 검증: RadCalNet Baotou(BTCN) 자료 적용 사례)

  • Kim, Kwangseob;Lee, Kiwon
    • Korean Journal of Remote Sensing
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    • v.36 no.6_2
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    • pp.1509-1521
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    • 2020
  • Experiments for validation of surface reflectance produced by Korea Multi-Purpose Satellite (KOMPSAT-3A) were conducted using Chinese Baotou (BTCN) data among four sites of the Radical Calibration Network (RadCalNet), a portal that provides spectrophotometric reflectance measurements. The atmosphere reflectance and surface reflectance products were generated using an extension program of an open-source Orfeo ToolBox (OTB), which was redesigned and implemented to extract those reflectance products in batches. Three image data sets of 2016, 2017, and 2018 were taken into account of the two sensor model variability, ver. 1.4 released in 2017 and ver. 1.5 in 2019, such as gain and offset applied to the absolute atmospheric correction. The results of applying these sensor model variables showed that the reflectance products by ver. 1.4 were relatively well-matched with RadCalNet BTCN data, compared to ones by ver. 1.5. On the other hand, the reflectance products obtained from the Landsat-8 by the USGS LaSRC algorithm and Sentinel-2B images using the SNAP Sen2Cor program were used to quantitatively verify the differences in those of KOMPSAT-3A. Based on the RadCalNet BTCN data, the differences between the surface reflectance of KOMPSAT-3A image were shown to be highly consistent with B band as -0.031 to 0.034, G band as -0.001 to 0.055, R band as -0.072 to 0.037, and NIR band as -0.060 to 0.022. The surface reflectance of KOMPSAT-3A also indicated the accuracy level for further applications, compared to those of Landsat-8 and Sentinel-2B images. The results of this study are meaningful in confirming the applicability of Analysis Ready Data (ARD) to the surface reflectance on high-resolution satellites.

Evaluation the Feed Value of Whole Crop Rice Silage and Comparison of Rumen Fermentation according to Its Ratio (신규 조사료원 사료용 벼 사일리지의 사료가치 평가 및 급여 비율에 따른 반추위 발효성상 비교)

  • Park, Seol Hwa;Baek, Youl Chang;Lee, Seul;Kim, Byeong Hyeon;Ryu, Chae Hwa
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.4
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    • pp.236-243
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    • 2020
  • This study was to evaluate the feed value of whole crop rice silage (WCRS) and to investigate a suitable ratio of the WCRS and concentrate by an analysis of rumen fermentation. A total of 6 treatments were used according to WCRS: concentrate ratio on in vitro rumen fermentation: T1 (100:0), T2 (60:40), T3 (40:60), T4 (20:80), T5 (10:90), and T6 (0:100). The ruminal pH, total gas emission, ammonia nitrogen, and volatile fatty acid (VFA) were determined as fermentation parameters. Total nutrients digestibility trial was conducted by 4 treatments according to WCRS: concentrate ratio at 40:60 (W40), 20:80 (W20), and 10:90 (W10), respectively. Feed value was analyzed according to AOAC (2019) and nutrient digestibility was calculated based on NRC (2001). The levels of crude protein (CP), crude fat, and neutral detergent fiber of the WCRS were 12.29%, 1.67%, and 59.79%, respectively. It was found to be 51.49% as a result of predicting the total digestible nutrient of WCRS using the NRC (2001) model. In vitro rumen fermentation, T4, T5, and T6 treatments showed a greater gas emission and total VFA concentration compared with other treatments (p<0.05). Acetate and acetate to propionate ratio of T4, T5, and T6 were significantly higher than other treatments (p<0.05). There was a significant difference in the level of propionate and butyrate according to the WCRS: concentrate ratio (p<0.05). The digestibility of dry matter and CP was significantly lower in W40 than in other treatments (p<0.05); however, there was no difference in W20 and W10. In conclusion, the 20:80 (WCRS: concentrate) is beneficial for stabilizing the rumen that does not inhibit rumen fermentation and nutrient digestion. This ratio might have a positive effect on the economics of farms as a valuable feed.

Production and Fuel Properties of Wood Chips from Logging Residues by Timber Harvesting Methods (목재수확 방법에 따른 벌채부산물 목재칩의 생산 및 연료 특성)

  • Choi, Yun-Sung;Jeong, In-Seon;Cho, Min-Jae;Mun, Ho-Seong;Oh, Jae-Heun
    • Journal of Korean Society of Forest Science
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    • v.110 no.2
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    • pp.217-232
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    • 2021
  • This study calculated the productivity and cost of extraction and processing of logging residues by cut-to-length (CTL) and whole-tree (WT) harvesting methods. In addition, the comparative analysis of the characteristics of wood chip fuel to examine whether it was suitable for the fuel conditions of the energy facility. In the harvesting and processing system to produce the wood chips of logging residues the system productivity and cost of the CTL harvesting system were 1.6 Gwt/SMH and 89,865 won/Gwt, respectively. The productivity and cost of the WT harvesting system were 2.9 Gwt/SMH and 72,974 won/Gwt, respectively. The WT harvesting productivity increased 1.3times while harvesting cost decreased by 18.7% compared to the CTL harvesting system. The logging residues of wood chips were not suitable for CTL wood chips based on International Organization for Standardization (ISO 17225-4:2021) and South Korea standard (NIFoS, 2020), but the quality (A2, Second class) was improved through screening operation. The WT-unscreened wood chips conformed to NIFoS standard (second class) and did not conform to ISO but were improved through screening operation (Second class). In addition to the energy facility in plant A, all wood chips except CTL-unscreened wood chips were available through drying processing. The WT-unscreened wood chips were the lowest at 99,408 won/Gwt. Plants B, C, and D had higher moisture content than plant A, so WT-unscreened wood chips without drying processing were the lowest at 57,204 won/Gwt. Therefore, the production of logging residues should improve with operation methods that improve the quality of wood chips required for applying the variable biomass and energy facility.

Antioxidant Activity of Asteraceae Plant Seed Extracts (14종 국화과(Asteraceae) 식물 종자 추출물의 항산화 활성)

  • Kim, JunHyeok;Lee, Da Hyun;Lee, Mi Hyun;Jung, Young Ho;Park, Cho Hee;Lee, Hee Ho;Na, Chae Sun
    • Journal of Life Science
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    • v.31 no.6
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    • pp.543-549
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    • 2021
  • Approximately 10% of all angiosperms belong to the Asteraceae family. Plant species belonging to this family have traditionally been used as medicinal plants in the Korean Peninsula. We investigated the antioxidant activity of seed extracts from 14 species belonging to the Asteraceae family. Seeds with ≥ 90% percentage of filled seed and ≥ 50% final germination were used. The total phenolic content was the highest in Dendranthema zawadskii var. tenuisectum (13.5 mg of gallic acid equivalents (GAEs)/g seeds), followed by Dendranthema zawadskii var. latilobum (11.8 mg of GAEs/g seeds), and Callistephus chinensis (11.0 mg of GAEs/g seeds). The total flavonoid content was highest in C. chinensis (9.8 mg of quercetin equivalents (QEs)/g seeds), followed by D. zawadskii var. tenuisectum (7.2 mg of QEs/g seeds) and Taraxacum officinale (6.3 mg of QEs/g seeds). Our results showed that 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity was highest in D. zawadskii var. tenuisectum (57.4 ㎍/ml), followed by T. officinale (59.1 ㎍/ml) and D. zawadskii var. latilobum (65.0 ㎍/ml), with a half maximal inhibitory concentration (IC50) of DPPH scavenging activity. Furthermore, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity was highest in C. chinensis (26.2 ㎍/ml), followed by D. zawadskii var. tenuisectum (38.4 ㎍/ml), T. officinale (40.2 ㎍/ml), with a half maximal inhibitory concentration (IC50) of ABTS scavenging activity. Based on a cluster analysis according to the antioxidant activity, the 14 species were classified into five groups, with group 4 having the highest antioxidant activity and group 0 having the lowest antioxidant activity. D. zawadskii var. latilobum, D. zawadskii var. tenuisectum, T. officinale, and C. chinensis belonging to groups 3 and 4, exhibited high phenolic content and antioxidant activity and can be considered potent plant-derived natural antioxidants.

Effects of Elevated Temperature after the Booting Stage on Physiological Characteristics and Grain Development in Wheat (밀에서 출수 후 잎의 생리적 특성 및 종실 생장에 대한 수잉기 이후 고온의 효과)

  • Song, Ki Eun;Choi, Jae Eun;Jung, Jae Gyeong;Ko, Jong Han;Lee, Kyung Do;Shim, Sang-In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.4
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    • pp.307-317
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    • 2021
  • In recent years, global warming has led to frequent climate change-related problems, and elevated temperatures, among adverse climatic factors, represent a critical problem negatively affecting crop growth and yield. In this context, the present study examined the physiological traits of wheat plants grown under high temperatures. Specifically, the effects of elevated temperatures on seed development after heading were evaluated, and the vegetation indices of different organs were assessed using hyperspectral analysis. Among physiological traits, leaf greenness and OJIP parameters were higher in the high-temperature treatment than in the control treatment. Similarly, the leaf photosynthetic rate during seed development was higher in the high-temperature treatment than in the control treatment. Moreover, temperature by organ was higher in the high-temperature treatment than in the control treatment; consequently, the leaf transpiration rate and stomatal conductance were higher in the control treatment than in the high-temperature treatment. On all measuring dates, the weight of spikes and seeds corresponding to the sink organs was greater in the high-temperature treatment than in the control treatment. Additionally, the seed growth rate was higher in the high-temperature treatment than in the control treatment 14 days after heading, which may be attributed to the higher redistribution of photosynthates at the early stage of seed development in the former. In hyperspectral analysis, the vegetation indices related to leaf chlorophyll content and nitrogen state were higher in the high-temperature treatment than in the control treatment after heading. Our results suggest that elevated temperatures after the booting stage positively affect wheat growth and yield.

Changes in Growth and Yield of Different Rice Varieties under Different Planting Densities in Low-Density Transplanting Cultivation (벼 드문모심기 재식밀도에 따른 품종별 생육 및 수량 변이)

  • Yang, SeoYeong;Hwang, WoonHa;Jeong, JaeHyeok;Lee, HyeonSeok;Lee, ChungGeun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.4
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    • pp.279-288
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    • 2021
  • Low-density transplanting is a cultivation technology that reduces labor and production costs. In this study, the growth and yield of several varieties with different tillering characteristics were analyzed in order to establish an appropriate planting density for low-density transplanting. Varieties with Low-Tillering (LT), Medium-Tillering (MT), and High-Tillering (HT) were planted at a density of 37-80 hills/3.3 m2. As the planting density decreased, the number of tillers per hill increased, but the number of tillers per square meter of hill decreased, especially for the LT variety. Decreasing density extended the tillering stage, which was longest in the LT variety. As the planting density decreased, SPAD(Soil plant analysis development, chlorophyll meter) values just before heading increased while canopy light interception decreased. Such changes were much greater in the LT variety than in the MT and HT varieties. The heading date tended to be delayed by 0-2 days as the planting density decreased, and there was no difference in the length of the period from first heading to full heading. As the number of spikelets per panicle increased, the number of spikelets per square meter did not differ according to the planting density. Decreasing planting density did not affect the grain weight; nevertheless, the yield ultimately decreased because of the decreasing ripening rate. The optimal planting density for stable low-density transplanting cultivation was determined to be over 50 hills/3.3 m2. In addition, these results suggest that LT varieties should be avoided, since these showed large decreases in growth and yield with decreasing planting density.

Investigation of microbial contamination on manufacturing processes for small-scale Korean traditional cookies manufacturers (소규모 한과제조업체의 제조공정에 대한 미생물 오염 조사)

  • Kim Sol-A;Lee, Jeong-Eun;Park, Hyun-Jin;Park, Mi-Seon;Choi, Song Yi;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.36 no.6
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    • pp.493-503
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    • 2021
  • The study was designed to analyze raw and auxiliary materials of Korean traditional cookies such as Yugwa and Gangjeong, equipment and tools, personal hygiene of workers and microbial contamination of materials by each manufacturing process. In addition, it looked at washing method for reducing microorganisms at the site and reduction effect of microorganisms by frequency in the manufacturing processes of Yugwa. In the process of producing Korean traditional cookies, the level of total aerobic bacteria (TAB) in popped rice was 1.2 Log CFU/g and the level of TAB in finished products increased to 3.7 Log CFU/g. In the process of producing Yugwa, the level of TAB increased to a maximum of 6.5 Log CFU/g in the soaking process but decreased to 1.3 Log CFU/g in the frying process. However, the level of TAB increased again to 1.3 Log CFU/g in finished products that proves its recontamination. It is estimated that he manufacturing process causes cross-contamination that comes from the work tools, equipment or workers. In particular, the spatula, one of the work tools, was found to have 4.4 Log CFU/g of aerobic bacteria and 4.2 Log CFU/g of colon bacillus that show they are highly contaminated. In the soaking process of Yugwa that lasts seven days, the level of TAB was a maximum of 10 Log CFU/g and the level of total colon bacillus was 6.8 Log CFU/g. When compared with washing methods, using hands and tools or running water, it is confirmed that the level of both TAB and total colon bacillus decreased to 5.0 Log CFU/g and 2.8 Log CFU/g respectively when hands were washed with running water 10 times. The above result shows that it's required for workers to wash their hands as well as wash and disinfect work tools and equipment in the process of producing Korean traditional cookies at small-scale companies. In addition, to reduce the level of microbial contamination in finished products, workers are required to apply their reduction method at the site.

Anti-obesity effect of radish leaf extracts on high fat diet-induced obesity in mice (고지방식이를 통해 비만이 유발된 마우스에서 무청 추출물의 항비만 효과)

  • Lee, Yun-Seong;Seo, Young Ho;Kim, Ji Yong
    • Korean Journal of Food Science and Technology
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    • v.54 no.3
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    • pp.297-305
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    • 2022
  • The goal of this study was to evaluate the anti-obesity effect of radish leaf extracts (MU-C) and radish leaf extracts with 3% citric acid (MU-CA) in a high-fat diet (HFD)-induced C57BL/6 mice. The effects of radish leaf extracts on adipogenesis were also investigated using 3T3-L1 adipocytes. As determined by Oil red O staining, MU-C inhibited adipogenesis in 3T3-L1 adipocytes. Four-week-old male C57BL/6 mice were fed an HFD for 6 weeks and then treated with radish leaf extracts (500 mg/kg, p.o.) for 6 weeks. Then, the serum levels of Aspartate aminotransferase, Alanine aminotransferase, Total cholesterol, Triglyceride and low-density lipoprotein cholesterol in the mice were measured using an automatic chemical analyzer and enzyme-linked immunosorbent assay. Administration of MU-C significantly reduced the fat weight when compared with HFD controls. As confirmed by histopathologic analysis, adipose tissue size markedly decreased in mice treated with MU-C. Therefore, this study could provide a basis for investigating the clinical use of MU-C as an agent for preventing obesity.